UniProtKB - Q7TPR4 (ACTN1_MOUSE)
Protein
Alpha-actinin-1
Gene
Actn1
Organism
Mus musculus (Mouse)
Status
Functioni
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 759 – 770 | 1PROSITE-ProRule annotationAdd BLAST | 12 | |
Calcium bindingi | 800 – 811 | 2PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- actin filament binding Source: UniProtKB
- calcium ion binding Source: InterPro
- cytoskeletal regulatory protein binding Source: MGI
- double-stranded RNA binding Source: MGI
- integrin binding Source: UniProtKB
- ion channel binding Source: MGI
- nuclear receptor transcription coactivator activity Source: MGI
- protein domain specific binding Source: MGI
- protein homodimerization activity Source: UniProtKB
- structural constituent of postsynapse Source: MGI
- vinculin binding Source: UniProtKB
GO - Biological processi
- actin filament bundle assembly Source: UniProtKB
- actin filament network formation Source: MGI
- actin filament organization Source: MGI
- cortical cytoskeleton organization Source: UniProtKB
- focal adhesion assembly Source: MGI
- negative regulation of cellular component movement Source: MGI
- platelet formation Source: MGI
- platelet morphogenesis Source: MGI
Keywordsi
Molecular function | Actin-binding |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-446388, Regulation of cytoskeletal remodeling and cell spreading by IPP complex components |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-actinin-1Alternative name(s): Alpha-actinin cytoskeletal isoform F-actin cross-linking protein Non-muscle alpha-actinin-1 |
Gene namesi | Name:Actn1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2137706, Actn1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Cytoskeleton
- cytoskeleton 1 Publication
Other locations
- Z line 1 Publication
- Cell junction By similarity
- ruffle 1 Publication
Note: Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle (PubMed:11114196). Colocalizes with PSD in membrane ruffles and central reticular structures (PubMed:17298598).2 Publications
Cytoskeleton
- actin filament Source: Ensembl
- cortical cytoskeleton Source: UniProtKB
- cytoskeleton Source: MGI
- stress fiber Source: MGI
Nucleus
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- brush border Source: UniProtKB
- cell junction Source: MGI
- cell projection Source: MGI
- cell-cell junction Source: MGI
- cytoplasm Source: MGI
- dendritic spine Source: MGI
- dense core granule membrane Source: UniProtKB
- fascia adherens Source: MGI
- focal adhesion Source: MGI
- glutamatergic synapse Source: MGI
- ruffle Source: UniProtKB
- sarcomere Source: MGI
- secretory granule Source: UniProtKB
- Z disc Source: MGI
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 32 | Q → K: Shows less organization of the circumferential actin-filament network compared to controls. 1 Publication | 1 | |
Mutagenesisi | 105 | V → I: Shows less organization of the circumferential actin-filament network compared to controls. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000073432 | 1 – 892 | Alpha-actinin-1Add BLAST | 892 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 6 | PhosphoserineBy similarity | 1 | |
Modified residuei | 12 | Phosphotyrosine; by FAK1By similarity | 1 | |
Modified residuei | 95 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 195 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 471 | PhosphoserineBy similarity | 1 | |
Modified residuei | 676 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 677 | PhosphoserineBy similarity | 1 | |
Modified residuei | 890 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3764 |
EPDi | Q7TPR4 |
jPOSTi | Q7TPR4 |
MaxQBi | Q7TPR4 |
PaxDbi | Q7TPR4 |
PeptideAtlasi | Q7TPR4 |
PRIDEi | Q7TPR4 |
PTM databases
CarbonylDBi | Q7TPR4 |
iPTMneti | Q7TPR4 |
PhosphoSitePlusi | Q7TPR4 |
SwissPalmi | Q7TPR4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000015143, Expressed in aorta and 308 other tissues |
ExpressionAtlasi | Q7TPR4, baseline and differential |
Genevisiblei | Q7TPR4, MM |
Interactioni
Subunit structurei
Homodimer; antiparallel.
Interacts with MYOZ2, TTID and LPP.
Interacts with DDN (By similarity).
Interacts with PSD (PubMed:17298598).
Interacts with MICALL2 (PubMed:23100251).
Interacts with DNM2 and CTTN.
Interacts with PDLIM1.
Interacts with PDLIM2.
Interacts with PDLIM4 (via PDZ domain) (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- actin filament binding Source: UniProtKB
- cytoskeletal regulatory protein binding Source: MGI
- integrin binding Source: UniProtKB
- ion channel binding Source: MGI
- protein domain specific binding Source: MGI
- protein homodimerization activity Source: UniProtKB
- vinculin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 224977, 35 interactors |
CORUMi | Q7TPR4 |
IntActi | Q7TPR4, 18 interactors |
MINTi | Q7TPR4 |
STRINGi | 10090.ENSMUSP00000021554 |
Miscellaneous databases
RNActi | Q7TPR4, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q7TPR4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 31 – 135 | Calponin-homology (CH) 1PROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 144 – 250 | Calponin-homology (CH) 2PROSITE-ProRule annotationAdd BLAST | 107 | |
Repeati | 274 – 384 | Spectrin 1Add BLAST | 111 | |
Repeati | 394 – 499 | Spectrin 2Add BLAST | 106 | |
Repeati | 509 – 620 | Spectrin 3Add BLAST | 112 | |
Repeati | 630 – 733 | Spectrin 4Add BLAST | 104 | |
Domaini | 746 – 781 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 787 – 822 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 247 | Actin-bindingAdd BLAST | 247 | |
Regioni | 274 – 733 | Interaction with DDNBy similarityAdd BLAST | 460 |
Sequence similaritiesi
Belongs to the alpha-actinin family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0035, Eukaryota |
GeneTreei | ENSGT00940000155548 |
InParanoidi | Q7TPR4 |
OMAi | QRFENVN |
OrthoDBi | 543832at2759 |
PhylomeDBi | Q7TPR4 |
TreeFami | TF352676 |
Family and domain databases
CDDi | cd00014, CH, 2 hits cd00051, EFh, 1 hit |
Gene3Di | 1.10.418.10, 2 hits |
InterProi | View protein in InterPro IPR001589, Actinin_actin-bd_CS IPR001715, CH-domain IPR036872, CH_dom_sf IPR011992, EF-hand-dom_pair IPR014837, EF-hand_Ca_insen IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR018159, Spectrin/alpha-actinin IPR002017, Spectrin_repeat |
Pfami | View protein in Pfam PF00307, CH, 2 hits PF08726, EFhand_Ca_insen, 1 hit PF00435, Spectrin, 4 hits |
SMARTi | View protein in SMART SM00033, CH, 2 hits SM00054, EFh, 2 hits SM00150, SPEC, 2 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF47576, SSF47576, 1 hit |
PROSITEi | View protein in PROSITE PS00019, ACTININ_1, 1 hit PS00020, ACTININ_2, 1 hit PS50021, CH, 2 hits PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q7TPR4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT
60 70 80 90 100
QIENIEEDFR DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI
110 120 130 140 150
ASKGVKLVSI GAEEIVDGNV KMTLGMIWTI ILRFAIQDIS VEETSAKEGL
160 170 180 190 200
LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC ALIHRHRPEL IDYGKLRKDD
210 220 230 240 250
PLTNLNTAFD VAERFLDIPK MLDAEDIVGT ARPDEKAIMT YVSSFYHAFS
260 270 280 290 300
GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR
310 320 330 340 350
VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR
360 370 380 390 400
PAFMPSEGRM VSDINNAWGC LEQAEKGYEE WLLNEIRRLE RLDHLAEKFR
410 420 430 440 450
QKASIHEAWT DGKEAMLRQK DYETATLSEI KALLKKHEAF ESDLAAHQDR
460 470 480 490 500
VEQIAAIAQE LNELDYYDSP SVNARCQKIC DQWDNLGALT QKRREALERT
510 520 530 540 550
EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT IEEIQGLTTA
560 570 580 590 600
HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI
610 620 630 640 650
NGKWDHVRQL VPRRDQALTE EHARQQHNER LRKQFGAQAN VIGPWIQTKM
660 670 680 690 700
EEIGRISIEM HGTLEDQLSH LRQYEKSIVN YKPKIDQLEC DHQLIQEALI
710 720 730 740 750
FDNKHTNYNM EHIRVGWEQL LTTIARTINE VENQILTRDA KGISQEQMNE
760 770 780 790 800
FRASFNHFDR DHSGTLGPEE FKACLISLGY DIGNDPQGEA EFARIMSIVD
810 820 830 840 850
PNRLGVVTFQ AFIDFMSRET ADTDTADQVM ASFKILAGDK NYITEDELRR
860 870 880 890
ELPPDQAEYC IARMAPYAGP DSVPGALDYM SFSTALYGES DL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA1BN54 | A1BN54_MOUSE | Alpha actinin 1a | Actn1 Actn1a | 887 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC054830 mRNA Translation: AAH54830.1 |
CCDSi | CCDS26011.1 |
RefSeqi | NP_598917.1, NM_134156.2 |
Genome annotation databases
Ensembli | ENSMUST00000021554; ENSMUSP00000021554; ENSMUSG00000015143 |
GeneIDi | 109711 |
KEGGi | mmu:109711 |
UCSCi | uc007oap.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC054830 mRNA Translation: AAH54830.1 |
CCDSi | CCDS26011.1 |
RefSeqi | NP_598917.1, NM_134156.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6C0A | NMR | - | A | 822-892 | [»] | |
SMRi | Q7TPR4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 224977, 35 interactors |
CORUMi | Q7TPR4 |
IntActi | Q7TPR4, 18 interactors |
MINTi | Q7TPR4 |
STRINGi | 10090.ENSMUSP00000021554 |
PTM databases
CarbonylDBi | Q7TPR4 |
iPTMneti | Q7TPR4 |
PhosphoSitePlusi | Q7TPR4 |
SwissPalmi | Q7TPR4 |
Proteomic databases
CPTACi | non-CPTAC-3764 |
EPDi | Q7TPR4 |
jPOSTi | Q7TPR4 |
MaxQBi | Q7TPR4 |
PaxDbi | Q7TPR4 |
PeptideAtlasi | Q7TPR4 |
PRIDEi | Q7TPR4 |
Protocols and materials databases
Antibodypediai | 51, 462 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000021554; ENSMUSP00000021554; ENSMUSG00000015143 |
GeneIDi | 109711 |
KEGGi | mmu:109711 |
UCSCi | uc007oap.2, mouse |
Organism-specific databases
CTDi | 87 |
MGIi | MGI:2137706, Actn1 |
Phylogenomic databases
eggNOGi | KOG0035, Eukaryota |
GeneTreei | ENSGT00940000155548 |
InParanoidi | Q7TPR4 |
OMAi | QRFENVN |
OrthoDBi | 543832at2759 |
PhylomeDBi | Q7TPR4 |
TreeFami | TF352676 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-446388, Regulation of cytoskeletal remodeling and cell spreading by IPP complex components |
Miscellaneous databases
BioGRID-ORCSi | 109711, 0 hits in 18 CRISPR screens |
ChiTaRSi | Actn1, mouse |
PROi | PR:Q7TPR4 |
RNActi | Q7TPR4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015143, Expressed in aorta and 308 other tissues |
ExpressionAtlasi | Q7TPR4, baseline and differential |
Genevisiblei | Q7TPR4, MM |
Family and domain databases
CDDi | cd00014, CH, 2 hits cd00051, EFh, 1 hit |
Gene3Di | 1.10.418.10, 2 hits |
InterProi | View protein in InterPro IPR001589, Actinin_actin-bd_CS IPR001715, CH-domain IPR036872, CH_dom_sf IPR011992, EF-hand-dom_pair IPR014837, EF-hand_Ca_insen IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR018159, Spectrin/alpha-actinin IPR002017, Spectrin_repeat |
Pfami | View protein in Pfam PF00307, CH, 2 hits PF08726, EFhand_Ca_insen, 1 hit PF00435, Spectrin, 4 hits |
SMARTi | View protein in SMART SM00033, CH, 2 hits SM00054, EFh, 2 hits SM00150, SPEC, 2 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF47576, SSF47576, 1 hit |
PROSITEi | View protein in PROSITE PS00019, ACTININ_1, 1 hit PS00020, ACTININ_2, 1 hit PS50021, CH, 2 hits PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACTN1_MOUSE | |
Accessioni | Q7TPR4Primary (citable) accession number: Q7TPR4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | October 1, 2003 | |
Last modified: | February 10, 2021 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families