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Protein

Integrator complex subunit 3

Gene

Ints3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Integrator (INT) complex. The Integrator complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.By similarity
Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single-stranded DNA at DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. The SOSS complex is required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways. In the SOSS complex, it is required for the assembly of the complex and for stabilization of the complex at DNA damage sites.By similarity

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-MMU-6807505 RNA polymerase II transcribes snRNA genes

Names & Taxonomyi

Protein namesi
Recommended name:
Integrator complex subunit 3
Short name:
Int3
Alternative name(s):
SOSS complex subunit A
Sensor of single-strand DNA complex subunit A
Short name:
SOSS-A
Short name:
Sensor of ssDNA subunit A
Gene namesi
Name:Ints3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2140050 Ints3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002595351 – 1041Integrator complex subunit 3Add BLAST1041

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei500PhosphoserineCombined sources1
Modified residuei535PhosphoserineCombined sources1
Modified residuei993PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ7TPD0
PaxDbiQ7TPD0
PeptideAtlasiQ7TPD0
PRIDEiQ7TPD0

PTM databases

iPTMnetiQ7TPD0
PhosphoSitePlusiQ7TPD0

Expressioni

Gene expression databases

BgeeiENSMUSG00000027933 Expressed in 290 organ(s), highest expression level in paneth cell
CleanExiMM_INTS3
ExpressionAtlasiQ7TPD0 baseline and differential
GenevisibleiQ7TPD0 MM

Interactioni

Subunit structurei

Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, INTS11/CPSF3L and INTS12. Component of the SOSS complex, composed of SOSS-B (SOSS-B1/NABP2 or SOSS-B2/NABP1), SOSS-A/INTS3 and SOSS-C/INIP. SOSS complexes containing SOSS-B1/NABP2 are more abundant than complexes containing SOSS-B2/NABP1. Interacts with SOSS-B1/NABP2, SOSS-B2/NABP1 and SOSS-C/INIP; the interaction is direct. Interacts with NBN/NBS1.By similarity

Protein-protein interaction databases

BioGridi230859, 3 interactors
ComplexPortaliCPX-613 SOSS1 complex
CPX-615 SOSS2 complex
DIPiDIP-59970N
IntActiQ7TPD0, 3 interactors
MINTiQ7TPD0
STRINGi10090.ENSMUSP00000029542

Structurei

3D structure databases

ProteinModelPortaliQ7TPD0
SMRiQ7TPD0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 33Ala/Gly-richAdd BLAST25

Sequence similaritiesi

Belongs to the Integrator subunit 3 family.Curated

Phylogenomic databases

eggNOGiKOG4262 Eukaryota
ENOG410XRCW LUCA
GeneTreeiENSGT00390000014184
HOGENOMiHOG000113080
HOVERGENiHBG081800
InParanoidiQ7TPD0
KOiK13140
OMAiRFLQCRL
OrthoDBiEOG091G02QS
PhylomeDBiQ7TPD0
TreeFamiTF323623

Family and domain databases

InterProiView protein in InterPro
IPR019333 Int_cplx_su3
PANTHERiPTHR13587 PTHR13587, 1 hit
PfamiView protein in Pfam
PF10189 Ints3, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7TPD0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELQKGKGTV AAAASGAAGG GGGGAGAGAP GGGRLLLSTS LDAKDELEER
60 70 80 90 100
LERCMSIVTS MTAGVSEREA NDALNAYVCK GPPQHEEICL GLFTLVLTEP
110 120 130 140 150
AQAQKCYRDL ALVSRDGMNI VLNKINQLLM EKYLKLQDTC RTQLVWLVRE
160 170 180 190 200
LVKSGVLGAD GVCMTFMKQI AGGDVTAKNI WLAESVLDIL TEQREWVLKS
210 220 230 240 250
SILIAMAVYT YLRLIVDHHG TAQLQTLRQK EVDFCISLLR ERFMECLMIG
260 270 280 290 300
RDLVRLLQNV ARIPEFELLW KDIIHNPQAL SPQFTGILQL LQSRTSRKFL
310 320 330 340 350
ACRLTPDMET KLLFMTSRVR FGQQKRYQDW FQRQYLSTPD SQSLRCDLIR
360 370 380 390 400
YICGVVHPSN EVLSSDILPR WAIIGWLLTT CTSNVAASNA KLALFYDWLF
410 420 430 440 450
FSPEKDSIMN IEPAILVMHH SMKPHPAITA TLLDFMCRII PNFYPPLEGH
460 470 480 490 500
VRQGVFSSLN HIVEKRVLAH LAPLFDNPKL DKELRSMLRE KFPEFCSSPS
510 520 530 540 550
PPVEVKIEEP VSMEMDNHLS DKDESCYDNA EAAFSDDEED LNSKGKKREF
560 570 580 590 600
RFHPIKETVV EEPVDVTPYL DQLDESLRDK VLQLQKGSDT EAQCEVMQEI
610 620 630 640 650
VDQVLEEDFD SEQLSVLASC LQELFKAHFR GEVLPEEVTE ESLEESVGKP
660 670 680 690 700
LYLIFRNLCQ MQEDNSSFSL LLDLLSELYQ KQPKIGYHLL YYLRASKAAA
710 720 730 740 750
GKMNLYESFA QATQLGDLHT CLMMDMKACQ EDDVRLLCHL TPSIYTEFPD
760 770 780 790 800
ETLRSGELLN MIVAVIDSAQ LQELVCHVMM GNLVMFRKDS VLNILIQSLD
810 820 830 840 850
WETFEQYCAW QLFLAHNIPL ETIIPILQHL KYKEHPEALS CLLLQLRREK
860 870 880 890 900
PSEEMVKMVL SRPCHPDDQF TTSILRHWCM KHDELLAEHI KALLIKNNSL
910 920 930 940 950
PRKRQSLRSS SSKLAQLTLE QILEHLDNLR LNLANTKQNF FSQTPILQAL
960 970 980 990 1000
QHVQASCDEA HKMKFSDLFS LAEEYEDSST KPPKSRRKAA LSSPRSRKNA
1010 1020 1030 1040
TQPPNAEEES GSSSASEEED TKPKPTKRKR KGSSAVGSDS D
Length:1,041
Mass (Da):117,938
Last modified:September 22, 2009 - v2
Checksum:i852920EFDAA5688E
GO
Isoform 2 (identifier: Q7TPD0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     321-330: FGQQKRYQDW → SSVHRRRSYR
     331-1041: Missing.

Note: No experimental confirmation available.
Show »
Length:330
Mass (Da):36,655
Checksum:iFC2FCF811FEDCF53
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JFJ6A0A0G2JFJ6_MOUSE
Integrator complex subunit 3
Ints3
1,035Annotation score:

Sequence cautioni

The sequence BAC31492 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti553H → P in BAC31492 (PubMed:16141072).Curated1
Sequence conflicti948Q → H in AAH55344 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038133321 – 330FGQQKRYQDW → SSVHRRRSYR in isoform 2. 1 Publication10
Alternative sequenceiVSP_038134331 – 1041Missing in isoform 2. 1 PublicationAdd BLAST711

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK040147 mRNA Translation: BAC30523.1
AK043206 mRNA Translation: BAC31492.1 Different initiation.
BC003209 mRNA Translation: AAH03209.2
BC055344 mRNA Translation: AAH55344.1
CCDSiCCDS17528.1 [Q7TPD0-1]
RefSeqiNP_663515.2, NM_145540.3 [Q7TPD0-1]
NP_849207.2, NM_178876.3 [Q7TPD0-1]
UniGeneiMm.268564

Genome annotation databases

EnsembliENSMUST00000029542; ENSMUSP00000029542; ENSMUSG00000027933 [Q7TPD0-1]
ENSMUST00000071488; ENSMUSP00000071422; ENSMUSG00000027933 [Q7TPD0-1]
GeneIDi229543
KEGGimmu:229543
UCSCiuc008qcd.2 mouse [Q7TPD0-1]
uc008qcf.1 mouse [Q7TPD0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK040147 mRNA Translation: BAC30523.1
AK043206 mRNA Translation: BAC31492.1 Different initiation.
BC003209 mRNA Translation: AAH03209.2
BC055344 mRNA Translation: AAH55344.1
CCDSiCCDS17528.1 [Q7TPD0-1]
RefSeqiNP_663515.2, NM_145540.3 [Q7TPD0-1]
NP_849207.2, NM_178876.3 [Q7TPD0-1]
UniGeneiMm.268564

3D structure databases

ProteinModelPortaliQ7TPD0
SMRiQ7TPD0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230859, 3 interactors
ComplexPortaliCPX-613 SOSS1 complex
CPX-615 SOSS2 complex
DIPiDIP-59970N
IntActiQ7TPD0, 3 interactors
MINTiQ7TPD0
STRINGi10090.ENSMUSP00000029542

PTM databases

iPTMnetiQ7TPD0
PhosphoSitePlusiQ7TPD0

Proteomic databases

EPDiQ7TPD0
PaxDbiQ7TPD0
PeptideAtlasiQ7TPD0
PRIDEiQ7TPD0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029542; ENSMUSP00000029542; ENSMUSG00000027933 [Q7TPD0-1]
ENSMUST00000071488; ENSMUSP00000071422; ENSMUSG00000027933 [Q7TPD0-1]
GeneIDi229543
KEGGimmu:229543
UCSCiuc008qcd.2 mouse [Q7TPD0-1]
uc008qcf.1 mouse [Q7TPD0-2]

Organism-specific databases

CTDi65123
MGIiMGI:2140050 Ints3

Phylogenomic databases

eggNOGiKOG4262 Eukaryota
ENOG410XRCW LUCA
GeneTreeiENSGT00390000014184
HOGENOMiHOG000113080
HOVERGENiHBG081800
InParanoidiQ7TPD0
KOiK13140
OMAiRFLQCRL
OrthoDBiEOG091G02QS
PhylomeDBiQ7TPD0
TreeFamiTF323623

Enzyme and pathway databases

ReactomeiR-MMU-6807505 RNA polymerase II transcribes snRNA genes

Miscellaneous databases

PROiPR:Q7TPD0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027933 Expressed in 290 organ(s), highest expression level in paneth cell
CleanExiMM_INTS3
ExpressionAtlasiQ7TPD0 baseline and differential
GenevisibleiQ7TPD0 MM

Family and domain databases

InterProiView protein in InterPro
IPR019333 Int_cplx_su3
PANTHERiPTHR13587 PTHR13587, 1 hit
PfamiView protein in Pfam
PF10189 Ints3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiINT3_MOUSE
AccessioniPrimary (citable) accession number: Q7TPD0
Secondary accession number(s): A0A4X0, A0A4X3, Q99LK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: September 22, 2009
Last modified: October 10, 2018
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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