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Protein

Rho guanine nucleotide exchange factor 4

Gene

Arhgef4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion (By similarity). Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 4
Alternative name(s):
APC-stimulated guanine nucleotide exchange factor
Short name:
Asef
Gene namesi
Name:Arhgef4
Synonyms:Kiaa1112
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2442507 Arhgef4

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000809151 – 484Rho guanine nucleotide exchange factor 4Add BLAST484

Proteomic databases

MaxQBiQ7TNR9
PaxDbiQ7TNR9
PRIDEiQ7TNR9

PTM databases

PhosphoSitePlusiQ7TNR9

Expressioni

Tissue specificityi

Expressed in colon epithelial cells. Highly expressed in CNS, including hippocampus, olfactory bulb and cerebellum. Expression is aberrantly enhanced in most colorectal tumors.2 Publications

Gene expression databases

BgeeiENSMUSG00000037509 Expressed in 176 organ(s), highest expression level in lumbar subsegment of spinal cord
CleanExiMM_ARHGEF4
ExpressionAtlasiQ7TNR9 baseline and differential
GenevisibleiQ7TNR9 MM

Interactioni

Subunit structurei

Interacts with RHOA and RAC1, and APC. Found in a complex consisting of ARHGEF4, APC and CTNNB1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035980

Structurei

3D structure databases

ProteinModelPortaliQ7TNR9
SMRiQ7TNR9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 46SH3PROSITE-ProRule annotationAdd BLAST46
Domaini78 – 262DHPROSITE-ProRule annotationAdd BLAST185
Domaini293 – 400PHPROSITE-ProRule annotationAdd BLAST108

Domaini

In an autoinhibited form the SH3 domain binds intramolecularly to the DH domain, thus blocking the Rac-binding site.By similarity

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiENOG410KDSK Eukaryota
ENOG410XQDV LUCA
GeneTreeiENSGT00930000150835
HOGENOMiHOG000237363
HOVERGENiHBG050568
InParanoidiQ7TNR9
KOiK05769
PhylomeDBiQ7TNR9
TreeFamiTF316832

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00621 RhoGEF, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Q7TNR9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDDQELGFKA GDVIEVMDAT NREWWWGRVA DGEGWFPASF VRLRVNQDEP
60 70 80 90 100
ADDYEAPRAG AREADDSGPE AQSCKDQMRT NVINEILSTE RDYIKHLRDI
110 120 130 140 150
CEGYVRQCRK REDMFSEEQL RTIFGNIEDI YRCQKAFVKA LEQKFNTERP
160 170 180 190 200
HLSELGACFL EHQADFQIYS EYCNNHPNAC VELSRLTKLS KYVYFFEACR
210 220 230 240 250
LLQRMIDISL DGFLLTPVQK ICKYPLQLAE LLKYTHPQHR DFKNVEAALH
260 270 280 290 300
AMKNVAQLIN ERKRRLENID KIAQWQSSIE DWEGEDLLVR SSELIHSGEL
310 320 330 340 350
TRVTQPQARS QQRMFFLFDR QLIYCKKDLL RRDVLYYKGR LDMDDLEVVD
360 370 380 390 400
VEDGKDRDLH VSVKNAFRLY CGTTGDSHLL CARKPEQKQR WLKAFARERE
410 420 430 440 450
QVRLDQETGF SITELQRKQA MLNASKQQAT GKPKAVGRPG YLTRHKHPSL
460 470 480
PASRPQQQVL VLAEPRRKPS NFWHSISRLA PFRK
Length:484
Mass (Da):56,679
Last modified:September 27, 2004 - v2
Checksum:iD4DCDE8623F110FE
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PYK8E9PYK8_MOUSE
Rho guanine nucleotide exchange fac...
Arhgef4
1,855Annotation score:
E0CX56E0CX56_MOUSE
Rho guanine nucleotide exchange fac...
Arhgef4
628Annotation score:
E0CYG8E0CYG8_MOUSE
Rho guanine nucleotide exchange fac...
Arhgef4
216Annotation score:
E0CY60E0CY60_MOUSE
Rho guanine nucleotide exchange fac...
Arhgef4
273Annotation score:
A0A1B0GST8A0A1B0GST8_MOUSE
Rho guanine nucleotide exchange fac...
Arhgef4
56Annotation score:

Sequence cautioni

The sequence BC055804 differs from that shown. Reason: Frameshift at position 201.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC107738 Genomic DNA No translation available.
BC055804 mRNA No translation available.
AK122448 mRNA Translation: BAC65730.2
CCDSiCCDS14872.1
RefSeqiNP_898840.2, NM_183019.2
UniGeneiMm.41604

Genome annotation databases

EnsembliENSMUST00000047664; ENSMUSP00000035980; ENSMUSG00000037509
GeneIDi226970
KEGGimmu:226970
UCSCiuc007api.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC107738 Genomic DNA No translation available.
BC055804 mRNA No translation available.
AK122448 mRNA Translation: BAC65730.2
CCDSiCCDS14872.1
RefSeqiNP_898840.2, NM_183019.2
UniGeneiMm.41604

3D structure databases

ProteinModelPortaliQ7TNR9
SMRiQ7TNR9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035980

PTM databases

PhosphoSitePlusiQ7TNR9

Proteomic databases

MaxQBiQ7TNR9
PaxDbiQ7TNR9
PRIDEiQ7TNR9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047664; ENSMUSP00000035980; ENSMUSG00000037509
GeneIDi226970
KEGGimmu:226970
UCSCiuc007api.1 mouse

Organism-specific databases

CTDi50649
MGIiMGI:2442507 Arhgef4
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410KDSK Eukaryota
ENOG410XQDV LUCA
GeneTreeiENSGT00930000150835
HOGENOMiHOG000237363
HOVERGENiHBG050568
InParanoidiQ7TNR9
KOiK05769
PhylomeDBiQ7TNR9
TreeFamiTF316832

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events

Miscellaneous databases

ChiTaRSiArhgef4 mouse
PROiPR:Q7TNR9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037509 Expressed in 176 organ(s), highest expression level in lumbar subsegment of spinal cord
CleanExiMM_ARHGEF4
ExpressionAtlasiQ7TNR9 baseline and differential
GenevisibleiQ7TNR9 MM

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00621 RhoGEF, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARHG4_MOUSE
AccessioniPrimary (citable) accession number: Q7TNR9
Secondary accession number(s): Q80TJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 7, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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