UniProtKB - Q7TNC9 (I5P1_MOUSE)
Protein
Inositol polyphosphate-5-phosphatase A
Gene
Inpp5a
Organism
Mus musculus (Mouse)
Status
Functioni
Phosphatase that specifically hydrolyzes the 5-phosphate of inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate, and inositol 1,3,4,5-tetrasphosphate to inositol 1,3,4-trisphosphate (PubMed:26051944). Plays a crucial role in the survival of cerebellar Purkinje cells (PubMed:26051944).1 Publication
Catalytic activityi
- 1D-myo-inositol 1,4,5-trisphosphate + H2O = 1D-myo-inositol 1,4-bisphosphate + phosphate1 PublicationEC:3.1.3.561 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,3,4-trisphosphate + phosphate1 PublicationEC:3.1.3.561 PublicationThis reaction proceeds in the forward1 Publication direction.
GO - Molecular functioni
- inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity Source: UniProtKB-EC
- inositol-1,4,5-trisphosphate 5-phosphatase activity Source: UniProtKB-EC
- inositol-polyphosphate 5-phosphatase activity Source: UniProtKB
- PH domain binding Source: MGI
GO - Biological processi
- inositol phosphate dephosphorylation Source: MGI
- inositol phosphate-mediated signaling Source: GO_Central
- negative regulation of neuron death Source: UniProtKB
- phosphatidylinositol dephosphorylation Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
Reactomei | R-MMU-1855183, Synthesis of IP2, IP, and Ins in the cytosol |
Names & Taxonomyi
Protein namesi | Recommended name: Inositol polyphosphate-5-phosphatase A (EC:3.1.3.561 Publication)Alternative name(s): Type I inositol 1,4,5-trisphosphate 5-phosphatase Short name: 5PTase |
Gene namesi | Name:Inpp5a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2686961, Inpp5a |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity
Other locations
- dendrite 1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cell membrane, Cell projection, MembranePathology & Biotechi
Disruption phenotypei
Genetic deletion causes a perinatal lethal phenotype in most mutant mice (PubMed:26051944). A small percentage of mutants thrive and have a phenotype characterized by an ataxic gait and progressive Purkinje cell degeneration (PubMed:26051944). Purkinje cell death is spatially patterned with surviving Purkinje cells appearing normal and maintaining molecular layer morphology (PubMed:26051944). Phosphatase activity toward phosphoinositol substrates is reduced in the mutant relative to wild-type littermates (PubMed:26051944).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000451144 | 1 – 409 | Inositol polyphosphate-5-phosphatase AAdd BLAST | 409 | |
PropeptideiPRO_0000451145 | 410 – 412 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 409 | S-farnesyl cysteineBy similarity | 1 |
Post-translational modificationi
Isoprenylation at Cys-409 is required for localization at the membrane.By similarity
Keywords - PTMi
Lipoprotein, PrenylationProteomic databases
jPOSTi | Q7TNC9 |
MaxQBi | Q7TNC9 |
PaxDbi | E9QAS7 |
PeptideAtlasi | Q8BNK3 |
PRIDEi | Q7TNC9 |
PTM databases
SwissPalmi | Q7TNC9 |
Expressioni
Tissue specificityi
Expressed at high levels in cerebellar Purkinje cells (at protein level) (PubMed:26051944). Expressed in Sertoli cells of the testis (PubMed:31112734).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000025477, Expressed in cardiac ventricle and 314 other tissues |
Interactioni
Subunit structurei
Interacts with TASOR.
1 PublicationGO - Molecular functioni
- PH domain binding Source: MGI
Protein-protein interaction databases
IntActi | Q7TNC9, 1 interactor |
MINTi | Q8BNK3 |
Miscellaneous databases
RNActi | E9QAS7, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type I family.Curated
Phylogenomic databases
eggNOGi | KOG1976, Eukaryota |
HOGENOMi | CLU_057709_1_0_1 |
InParanoidi | E9QAS7 |
OMAi | CTGDHKP |
OrthoDBi | 642737at2759 |
TreeFami | TF314246 |
Family and domain databases
Gene3Di | 3.60.10.10, 1 hit |
InterProi | View protein in InterPro IPR036691, Endo/exonu/phosph_ase_sf IPR005135, Endo/exonuclease/phosphatase IPR039737, INPP5A IPR000300, IPPc |
PANTHERi | PTHR12997, PTHR12997, 1 hit |
Pfami | View protein in Pfam PF03372, Exo_endo_phos, 1 hit |
SMARTi | View protein in SMART SM00128, IPPc, 1 hit |
SUPFAMi | SSF56219, SSF56219, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q7TNC9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGKAAAPGT AVLLVTANVG SLFDDPENLQ KNWLREFYQV LHTHKPHFMA
60 70 80 90 100
LHCQEFGGKN YEASMSHVDK FVKELLSSDA MKEYNRARVY LDENYKSQEH
110 120 130 140 150
FTALGSFYFL HESLKNIYQF DFKAKKYKKV TGKEIYSDTL ESTPMLEKEK
160 170 180 190 200
FPQDYFPECK WSRKGFIRTR WCIADCAFDL VNIHLFHDAS NLVAWETSPS
210 220 230 240 250
VYSGVRHKAL GYVLDRIIDQ RFEKVSYFVF GDFNFRLDSK SVVETLCTKA
260 270 280 290 300
TMQTVRAADT NEVVKLIFRE SDNDRKVVLQ LEKKLFDYFN QDVFRDNNGT
310 320 330 340 350
ALLEFDKELS VFKDRLYELD ISFPPSYPYS EDSSQGEQYM NTRCPAWCDR
360 370 380 390 400
ILMSLSAKEL VLKSESEEKV ATYDHIGPNV CMGDHKPVFL AFRIAPGAGK
410
PHAHVHKCCV VQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ3TZT4 | Q3TZT4_MOUSE | Inositol-polyphosphate 5-phosphatas... | Inpp5a | 420 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 11 | A → V in BAC38919 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 27 | E → D in BAC38919 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 36 | E → G in BAC38919 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060756 | 401 – 412 | PHAHV…CCVVQ → RCQRRERILERPPCSSVSNS SS in isoform 2. CuratedAdd BLAST | 12 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK083449 mRNA Translation: BAC38919.1 AK171360 mRNA Translation: BAE42408.1 AK171880 mRNA Translation: BAE42716.1 AC112670 Genomic DNA No translation available. AC118735 Genomic DNA No translation available. BC056341 mRNA Translation: AAH56341.1 |
RefSeqi | NP_001120835.1, NM_001127363.1 [Q7TNC9-1] NP_898967.2, NM_183144.3 [Q7TNC9-2] |
Genome annotation databases
GeneIDi | 212111 |
KEGGi | mmu:212111 |
UCSCi | uc009kfr.2, mouse [Q7TNC9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK083449 mRNA Translation: BAC38919.1 AK171360 mRNA Translation: BAE42408.1 AK171880 mRNA Translation: BAE42716.1 AC112670 Genomic DNA No translation available. AC118735 Genomic DNA No translation available. BC056341 mRNA Translation: AAH56341.1 |
RefSeqi | NP_001120835.1, NM_001127363.1 [Q7TNC9-1] NP_898967.2, NM_183144.3 [Q7TNC9-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q7TNC9, 1 interactor |
MINTi | Q8BNK3 |
PTM databases
SwissPalmi | Q7TNC9 |
Proteomic databases
jPOSTi | Q7TNC9 |
MaxQBi | Q7TNC9 |
PaxDbi | E9QAS7 |
PeptideAtlasi | Q8BNK3 |
PRIDEi | Q7TNC9 |
Protocols and materials databases
Antibodypediai | 1413, 154 antibodies |
Genome annotation databases
GeneIDi | 212111 |
KEGGi | mmu:212111 |
UCSCi | uc009kfr.2, mouse [Q7TNC9-1] |
Organism-specific databases
CTDi | 3632 |
MGIi | MGI:2686961, Inpp5a |
Phylogenomic databases
eggNOGi | KOG1976, Eukaryota |
HOGENOMi | CLU_057709_1_0_1 |
InParanoidi | E9QAS7 |
OMAi | CTGDHKP |
OrthoDBi | 642737at2759 |
TreeFami | TF314246 |
Enzyme and pathway databases
Reactomei | R-MMU-1855183, Synthesis of IP2, IP, and Ins in the cytosol |
Miscellaneous databases
BioGRID-ORCSi | 212111, 1 hit in 17 CRISPR screens |
ChiTaRSi | Inpp5a, mouse |
RNActi | E9QAS7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025477, Expressed in cardiac ventricle and 314 other tissues |
Family and domain databases
Gene3Di | 3.60.10.10, 1 hit |
InterProi | View protein in InterPro IPR036691, Endo/exonu/phosph_ase_sf IPR005135, Endo/exonuclease/phosphatase IPR039737, INPP5A IPR000300, IPPc |
PANTHERi | PTHR12997, PTHR12997, 1 hit |
Pfami | View protein in Pfam PF03372, Exo_endo_phos, 1 hit |
SMARTi | View protein in SMART SM00128, IPPc, 1 hit |
SUPFAMi | SSF56219, SSF56219, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | I5P1_MOUSE | |
Accessioni | Q7TNC9Primary (citable) accession number: Q7TNC9 Secondary accession number(s): E9QAS7, Q8BNK3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 7, 2020 |
Last sequence update: | October 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families