UniProtKB - Q7TN79 (AKA7G_MOUSE)
Protein
A-kinase anchor protein 7 isoform gamma
Gene
Akap7
Organism
Mus musculus (Mouse)
Status
Functioni
Probably targets cAMP-dependent protein kinase (PKA) to the cellular membrane or cytoskeletal structures. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium (By similarity).By similarityCurated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 95 | AMPBy similarity | 1 | |
Binding sitei | 95 | CMPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 185 – 187 | AMPBy similarity | 3 | |
Nucleotide bindingi | 185 – 187 | CMPBy similarity | 3 |
GO - Molecular functioni
- AMP binding Source: MGI
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein kinase A binding Source: MGI
- protein kinase A regulatory subunit binding Source: UniProtKB
- protein kinase binding Source: MGI
GO - Biological processi
- cellular response to cAMP Source: MGI
- modulation of chemical synaptic transmission Source: SynGO
- positive regulation of delayed rectifier potassium channel activity Source: MGI
- positive regulation of potassium ion transmembrane transport Source: MGI
- protein localization Source: MGI
- regulation of membrane repolarization Source: MGI
- regulation of protein kinase A signaling Source: MGI
- transmembrane receptor protein serine/threonine kinase signaling pathway Source: MGI
Keywordsi
Ligand | Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: A-kinase anchor protein 7 isoform gammaBy similarityImportedShort name: AKAP-7 isoform gammaBy similarity Alternative name(s): A-kinase anchor protein 18By similarity Short name: AKAP-18By similarity Protein kinase A-anchoring protein 7 isoform gammaBy similarity Short name: PRKA7 isoform gammaBy similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1859150, Akap7 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- sarcoplasmic reticulum Source: MGI
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: MGI
- lateral plasma membrane Source: MGI
- T-tubule Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- exocytic vesicle Source: MGI
- hippocampal mossy fiber to CA3 synapse Source: SynGO
- membrane Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 41 | K → E: Abolishes nuclear localization; when associated with E-43. 1 Publication | 1 | |
Mutagenesisi | 43 | K → E: Abolishes nuclear localization; when associated with E-41. 1 Publication | 1 | |
Mutagenesisi | 265 | L → P: Abolishes PKA-RI-alpha binding; when associated with P-269. 1 Publication | 1 | |
Mutagenesisi | 269 | L → P: Abolishes PKA-RI-alpha binding; when associated with P-265. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000419634 | 1 – 314 | A-kinase anchor protein 7 isoform gammaAdd BLAST | 314 |
Proteomic databases
MaxQBi | Q7TN79 |
PaxDbi | Q7TN79 |
PeptideAtlasi | Q7TN79 |
PRIDEi | Q7TN79 |
PTM databases
iPTMneti | Q7TN79 |
Expressioni
Tissue specificityi
Expressed in oocytes.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000039166, Expressed in cerebral cortex and 249 other tissues |
ExpressionAtlasi | Q7TN79, baseline and differential |
Genevisiblei | Q7TN79, MM |
Interactioni
Subunit structurei
Binds cAMP-dependent protein kinase (PKA).
Interacts with PRKCA; only the cytoplasmic form is capable of interacting with PRKCA (By similarity).
By similarity1 PublicationGO - Molecular functioni
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein kinase A binding Source: MGI
- protein kinase A regulatory subunit binding Source: UniProtKB
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 240608, 1 interactor |
STRINGi | 10090.ENSMUSP00000043624 |
Miscellaneous databases
RNActi | Q7TN79, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 260 – 314 | PKA-RII-alpha subunit binding domainBy similarityAdd BLAST | 55 | |
Regioni | 261 – 285 | RI-alpha-binding1 PublicationAdd BLAST | 25 | |
Regioni | 262 – 275 | RII-bindingBy similarityAdd BLAST | 14 |
Phylogenomic databases
eggNOGi | KOG2814, Eukaryota |
GeneTreei | ENSGT00390000012756 |
HOGENOMi | CLU_052186_0_0_1 |
InParanoidi | Q7TN79 |
OMAi | MAEMPFA |
OrthoDBi | 606751at2759 |
TreeFami | TF105406 |
Family and domain databases
InterProi | View protein in InterPro IPR019511, AKAP7_RI-RII-bd_dom IPR009097, Cyclic_Pdiesterase IPR019510, Kinase-A_anchor_nucl_local_sig |
Pfami | View protein in Pfam PF10469, AKAP7_NLS, 1 hit PF10470, AKAP7_RIRII_bdg, 1 hit |
SUPFAMi | SSF55144, SSF55144, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform Gamma1 Publication (identifier: Q7TN79-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPFAAVDIQD DCGSPDVPQA NPKRSKEEEE DRGDKNDHVK KRKKAKKDYQ
60 70 80 90 100
PNYFLSIPIT NKKITTGIKV LQNSILQQDK RLTKAMVGDG SFHITLLVMQ
110 120 130 140 150
LLNEDEVNIG TDALLELKPF VEEILEGKHL ALPFQGIGTF QGQVGFVKLA
160 170 180 190 200
DGDHVSALLE IAETAKRTFR EKGILAGESR TFKPHLTFMK LSKAPMLRKK
210 220 230 240 250
GVRKIEPGLY EQFIDHRFGE ELLYQIDLCS MLKKKQSNGY YHCESSIVIG
260 270 280 290 300
EKDRREPEDA ELVRLSKRLV ENAVLKAVQQ YLEETQNKKQ PGEGNSTKAE
310
EGDRNGDGSD NNRK
Isoform AlphaCurated (identifier: O55074-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry O55074.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BJL8 | H3BJL8_MOUSE | A-kinase anchor protein 7 isoform a... | Akap7 | 141 | Annotation score: | ||
Q4KL21 | Q4KL21_MOUSE | A-kinase anchor protein 7 isoform a... | Akap7 | 122 | Annotation score: | ||
H3BJV2 | H3BJV2_MOUSE | A-kinase anchor protein 7 isoform a... | Akap7 | 126 | Annotation score: | ||
A0A1W2P7B3 | A0A1W2P7B3_MOUSE | A-kinase anchor protein 7 isoform a... | Akap7 | 95 | Annotation score: | ||
H3BKK9 | H3BKK9_MOUSE | A-kinase anchor protein 7 isoform a... | Akap7 | 19 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 70 | V → S in AAP55205 (PubMed:12804576).Curated | 1 | |
Sequence conflicti | 278 | V → A in AAP55205 (PubMed:12804576).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY301068 mRNA Translation: AAP55205.1 AC153549 Genomic DNA No translation available. AC153550 Genomic DNA No translation available. |
CCDSi | CCDS23753.1 [Q7TN79-1] |
RefSeqi | NP_061217.3, NM_018747.4 [Q7TN79-1] |
Genome annotation databases
Ensembli | ENSMUST00000041984; ENSMUSP00000043624; ENSMUSG00000039166 [Q7TN79-1] |
GeneIDi | 432442 |
UCSCi | uc007eri.1, mouse [Q7TN79-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY301068 mRNA Translation: AAP55205.1 AC153549 Genomic DNA No translation available. AC153550 Genomic DNA No translation available. |
CCDSi | CCDS23753.1 [Q7TN79-1] |
RefSeqi | NP_061217.3, NM_018747.4 [Q7TN79-1] |
3D structure databases
SMRi | Q7TN79 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 240608, 1 interactor |
STRINGi | 10090.ENSMUSP00000043624 |
PTM databases
iPTMneti | Q7TN79 |
Proteomic databases
MaxQBi | Q7TN79 |
PaxDbi | Q7TN79 |
PeptideAtlasi | Q7TN79 |
PRIDEi | Q7TN79 |
Protocols and materials databases
Antibodypediai | 19674, 280 antibodies |
DNASUi | 432442 |
Genome annotation databases
Ensembli | ENSMUST00000041984; ENSMUSP00000043624; ENSMUSG00000039166 [Q7TN79-1] |
GeneIDi | 432442 |
UCSCi | uc007eri.1, mouse [Q7TN79-1] |
Organism-specific databases
CTDi | 9465 |
MGIi | MGI:1859150, Akap7 |
Phylogenomic databases
eggNOGi | KOG2814, Eukaryota |
GeneTreei | ENSGT00390000012756 |
HOGENOMi | CLU_052186_0_0_1 |
InParanoidi | Q7TN79 |
OMAi | MAEMPFA |
OrthoDBi | 606751at2759 |
TreeFami | TF105406 |
Miscellaneous databases
BioGRID-ORCSi | 432442, 1 hit in 17 CRISPR screens |
ChiTaRSi | Akap7, mouse |
RNActi | Q7TN79, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039166, Expressed in cerebral cortex and 249 other tissues |
ExpressionAtlasi | Q7TN79, baseline and differential |
Genevisiblei | Q7TN79, MM |
Family and domain databases
InterProi | View protein in InterPro IPR019511, AKAP7_RI-RII-bd_dom IPR009097, Cyclic_Pdiesterase IPR019510, Kinase-A_anchor_nucl_local_sig |
Pfami | View protein in Pfam PF10469, AKAP7_NLS, 1 hit PF10470, AKAP7_RIRII_bdg, 1 hit |
SUPFAMi | SSF55144, SSF55144, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AKA7G_MOUSE | |
Accessioni | Q7TN79Primary (citable) accession number: Q7TN79 Secondary accession number(s): F8VQ58 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2012 |
Last sequence update: | October 3, 2012 | |
Last modified: | December 2, 2020 | |
This is version 112 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot