UniProtKB - Q7TN29 (SMAP2_MOUSE)
Protein
Stromal membrane-associated protein 2
Gene
Smap2
Organism
Mus musculus (Mouse)
Status
Functioni
GTPase activating protein that acts on ARF1. Can also activate ARF6 (in vitro). May play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi network.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 28 – 51 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- GTPase activator activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | GTPase activation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Stromal membrane-associated protein 2Alternative name(s): Stromal membrane-associated protein 1-like |
Gene namesi | Name:Smap2 Synonyms:Smap1l |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917030, Smap2 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 56 | R → Q: Loss of GTPase activation. 1 Publication | 1 | |
Mutagenesisi | 187 – 191 | LLGLD → AAAAA: Loss of interaction with clathrin heavy chains; when associated with 212-AAA-214. 1 Publication | 5 | |
Mutagenesisi | 212 – 214 | DLL → AAA: Loss of interaction with clathrin heavy chains; when associated with 187-AAAAA-191. 1 Publication | 3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000235842 | 1 – 428 | Stromal membrane-associated protein 2Add BLAST | 428 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 127 | PhosphoserineBy similarity | 1 | |
Modified residuei | 219 | PhosphoserineCombined sources | 1 | |
Modified residuei | 224 | PhosphoserineBy similarity | 1 | |
Modified residuei | 230 | PhosphoserineBy similarity | 1 | |
Modified residuei | 239 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q7TN29 |
jPOSTi | Q7TN29 |
PaxDbi | Q7TN29 |
PeptideAtlasi | Q7TN29 |
PRIDEi | Q7TN29 |
PTM databases
iPTMneti | Q7TN29 |
PhosphoSitePlusi | Q7TN29 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000032870, Expressed in saccule of membranous labyrinth and 294 other tissues |
Genevisiblei | Q7TN29, MM |
Interactioni
Subunit structurei
Interacts with ARF1.
Interacts with PICALM and clathrin heavy chains.
1 PublicationBinary interactionsi
Q7TN29
With | #Exp. | IntAct |
---|---|---|
Smap1 [Q91VZ6] | 4 | EBI-11358641,EBI-8317690 |
Protein-protein interaction databases
BioGRIDi | 213674, 2 interactors |
IntActi | Q7TN29, 1 interactor |
MINTi | Q7TN29 |
STRINGi | 10090.ENSMUSP00000035800 |
Miscellaneous databases
RNActi | Q7TN29, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 139 | Arf-GAPPROSITE-ProRule annotationAdd BLAST | 127 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 163 – 231 | Interaction with clathrin heavy chainsAdd BLAST | 69 | |
Regioni | 339 – 428 | Interaction with PICALM1 PublicationAdd BLAST | 90 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 278 – 410 | Met-richAdd BLAST | 133 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 28 – 51 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0703, Eukaryota |
GeneTreei | ENSGT00940000158387 |
HOGENOMi | CLU_023062_5_0_1 |
InParanoidi | Q7TN29 |
OMAi | QWNIAQM |
OrthoDBi | 1097163at2759 |
PhylomeDBi | Q7TN29 |
TreeFami | TF313876 |
Family and domain databases
Gene3Di | 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf |
Pfami | View protein in Pfam PF01412, ArfGap, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00105, ArfGap, 1 hit |
SUPFAMi | SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115, ARFGAP, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q7TN29-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTGKSVKDVD RYQAVLANLL LEEDNKFCAD CQSKGPRWAS WNIGVFICIR
60 70 80 90 100
CAGIHRNLGV HISRVKSVNL DQWTQEQIQC MQEMGNGKAN RLYEAYLPET
110 120 130 140 150
FRRPQIDPAV EGFIRDKYEK KKYMDRSLDI NVLRKEKDDK WKRGNEPAPE
160 170 180 190 200
KKMEPVVFEK VKMPQKKEDA QLPRKSSPKS AAPVMDLLGL DAPVACSIAN
210 220 230 240 250
SKTSNALEKD LDLLASVPSP SSVSRKAVGS MPTAGSAGSV PENLNLFPEP
260 270 280 290 300
GSKSEETGKK QLSKDSILSL YGSQTPQMPA QAMFMAPAQM AYPTAYPSFP
310 320 330 340 350
GVTPPNSIMG GMVPPPVGMV AQPGASGMLT PMAMPAGYMG GMQASMMGVP
360 370 380 390 400
NGMMTTQQAG YMASMAAMPQ TVYGVQPAQQ LQWNLTQMTQ QMAGMNFYGA
410 420
NGMMNYGQSM GGGNGQAANQ TLSPQMWK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 89 | A → V in AAH52413 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 179 | K → E in AAH52413 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018505 | 227 – 245 | AVGSM…PENLN → SQGANQKKQARNSSPRTPS in isoform 2. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_018506 | 246 – 428 | Missing in isoform 2. 1 PublicationAdd BLAST | 183 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK152757 mRNA Translation: BAE31472.1 BC052413 mRNA Translation: AAH52413.1 |
CCDSi | CCDS18598.1 [Q7TN29-1] |
RefSeqi | NP_598477.2, NM_133716.3 [Q7TN29-1] |
Genome annotation databases
Ensembli | ENSMUST00000043200; ENSMUSP00000035800; ENSMUSG00000032870 [Q7TN29-1] |
GeneIDi | 69780 |
KEGGi | mmu:69780 |
UCSCi | uc008unw.2, mouse [Q7TN29-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK152757 mRNA Translation: BAE31472.1 BC052413 mRNA Translation: AAH52413.1 |
CCDSi | CCDS18598.1 [Q7TN29-1] |
RefSeqi | NP_598477.2, NM_133716.3 [Q7TN29-1] |
3D structure databases
SMRi | Q7TN29 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 213674, 2 interactors |
IntActi | Q7TN29, 1 interactor |
MINTi | Q7TN29 |
STRINGi | 10090.ENSMUSP00000035800 |
PTM databases
iPTMneti | Q7TN29 |
PhosphoSitePlusi | Q7TN29 |
Proteomic databases
EPDi | Q7TN29 |
jPOSTi | Q7TN29 |
PaxDbi | Q7TN29 |
PeptideAtlasi | Q7TN29 |
PRIDEi | Q7TN29 |
Protocols and materials databases
Antibodypediai | 17974, 126 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000043200; ENSMUSP00000035800; ENSMUSG00000032870 [Q7TN29-1] |
GeneIDi | 69780 |
KEGGi | mmu:69780 |
UCSCi | uc008unw.2, mouse [Q7TN29-1] |
Organism-specific databases
CTDi | 64744 |
MGIi | MGI:1917030, Smap2 |
Phylogenomic databases
eggNOGi | KOG0703, Eukaryota |
GeneTreei | ENSGT00940000158387 |
HOGENOMi | CLU_023062_5_0_1 |
InParanoidi | Q7TN29 |
OMAi | QWNIAQM |
OrthoDBi | 1097163at2759 |
PhylomeDBi | Q7TN29 |
TreeFami | TF313876 |
Miscellaneous databases
BioGRID-ORCSi | 69780, 0 hits in 17 CRISPR screens |
ChiTaRSi | Smap2, mouse |
PROi | PR:Q7TN29 |
RNActi | Q7TN29, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032870, Expressed in saccule of membranous labyrinth and 294 other tissues |
Genevisiblei | Q7TN29, MM |
Family and domain databases
Gene3Di | 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf |
Pfami | View protein in Pfam PF01412, ArfGap, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00105, ArfGap, 1 hit |
SUPFAMi | SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115, ARFGAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SMAP2_MOUSE | |
Accessioni | Q7TN29Primary (citable) accession number: Q7TN29 Secondary accession number(s): Q3U798 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | October 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot