UniProtKB - Q7TMK6 (HOOK2_MOUSE)
Protein Hook homolog 2
Hook2
Functioni
GO - Molecular functioni
- dynein light intermediate chain binding Source: GO_Central
- identical protein binding Source: MGI
- microtubule binding Source: GO_Central
GO - Biological processi
- cytoplasmic microtubule organization Source: GO_Central
- cytoskeleton-dependent intracellular transport Source: GO_Central
- early endosome to late endosome transport Source: UniProtKB
- endosome organization Source: UniProtKB
- endosome to lysosome transport Source: UniProtKB
- lysosome organization Source: UniProtKB
- protein transport Source: UniProtKB-KW
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Protein Hook homolog 2Short name: mHK2 |
Gene namesi | Name:Hook2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2181664, Hook2 |
Subcellular locationi
Cytoskeleton
- centrosome 2 Publications
- cytoskeleton 2 Publications
Other locations
- Cytoplasm 1 Publication
Note: Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules (By similarity). Localizes to the manchette in elongating spermatids (PubMed:28003339).By similarity1 Publication
Cytoskeleton
- centrosome Source: MGI
- microtubule Source: UniProtKB-KW
Cytosol
- cytosol Source: MGI
Other locations
- cytoplasm Source: GO_Central
- FHF complex Source: UniProtKB
- HOPS complex Source: Ensembl
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219195 | 1 – 716 | Protein Hook homolog 2Add BLAST | 716 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 163 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q7TMK6 |
MaxQBi | Q7TMK6 |
PaxDbi | Q7TMK6 |
PRIDEi | Q7TMK6 |
PTM databases
iPTMneti | Q7TMK6 |
PhosphoSitePlusi | Q7TMK6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000052566, Expressed in midgut and 217 other tissues |
ExpressionAtlasi | Q7TMK6, baseline and differential |
Genevisiblei | Q7TMK6, MM |
Interactioni
Subunit structurei
Self-associates (By similarity).
Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3 (By similarity). May interact directly with AKTIP/FTS, HOOK1 and HOOK3 (By similarity). Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect (By similarity).
Interacts with CNTRL (By similarity).
Interacts with microtubules (By similarity).
Interacts with ZC3H14 (By similarity).
Interacts with LRGUK (via guanylate kinase-like domain) (PubMed:25781171, PubMed:28003339).
Interacts with CCDC181 (PubMed:28283191).
By similarity3 PublicationsGO - Molecular functioni
- dynein light intermediate chain binding Source: GO_Central
- identical protein binding Source: MGI
- microtubule binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 228463, 3 interactors |
IntActi | Q7TMK6, 3 interactors |
STRINGi | 10090.ENSMUSP00000067752 |
Miscellaneous databases
RNActi | Q7TMK6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 122 | Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST | 117 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 546 | Sufficient for interaction with microtubulesBy similarityAdd BLAST | 546 | |
Regioni | 1 – 161 | Required for localization to the centrosome and induction of aggresome formationBy similarityAdd BLAST | 161 | |
Regioni | 533 – 716 | Required for localization to the centrosome and induction of aggresome formationBy similarityAdd BLAST | 184 | |
Regioni | 582 – 716 | Sufficient for interaction with CNTRLBy similarityAdd BLAST | 135 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 188 – 427 | Sequence analysisAdd BLAST | 240 | |
Coiled coili | 455 – 605 | Sequence analysisAdd BLAST | 151 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QTJ1, Eukaryota |
GeneTreei | ENSGT00940000160152 |
HOGENOMi | CLU_011214_1_0_1 |
InParanoidi | Q7TMK6 |
OMAi | MLCAQEE |
OrthoDBi | 398210at2759 |
TreeFami | TF320231 |
Family and domain databases
Gene3Di | 1.10.418.10, 1 hit |
InterProi | View protein in InterPro IPR001715, CH-domain IPR036872, CH_dom_sf IPR008636, Hook_C IPR043936, HOOK_N |
Pfami | View protein in Pfam PF05622, HOOK, 1 hit PF19047, HOOK_N, 1 hit |
PROSITEi | View protein in PROSITE PS50021, CH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSVDKAELCG SLLTWLQTFQ VSPPCASPQD LSSGLAIAHV LNQIDPSWFN
60 70 80 90 100
NEWLQGISED SSPSWRLKVR KLEKILQSLV EYSKNVLGHP VSDQHLPDVS
110 120 130 140 150
LIGEFSNPAE LGKLLQLVLG CAISCEKKQE YIQRIMTLEE SVQHVVMEAI
160 170 180 190 200
QELMTKDTPD SLSPENYGNF DTQSRRYYFL SEEVEEGDHL QQHYLDLERQ
210 220 230 240 250
LLLLSEEKQN LAQENAALRE RVGRSEVESA PGLTAKKLLL LQSQLEQLQE
260 270 280 290 300
ENFRLESSRE DDRLRCLELE REVAELQQRN QALTSLSQEA QALKDEMDEL
310 320 330 340 350
RQSSERARQL EATLNSCRRR LGELQELRRQ VRQLEERNAG HAERTRQLEE
360 370 380 390 400
ELRRAGSLRA QLEAQRRQVQ ELQGQWQEEA MKAEKWLFEC RNLEEKCDLV
410 420 430 440 450
TKEKERLLTE RDSLREANEE LRCAQLQPRG LAQADLSLDP TPSGLENLAA
460 470 480 490 500
EILPAELRET LVRLQLENKR LCQQEAADRE RQEELQRHLE EANRARHGLE
510 520 530 540 550
AQQRLNQQQL SELRAQVEEL QKALQEQGGK TEDPTLLKRK LEDHLQKLHE
560 570 580 590 600
ADLELQRKRE YIEELEPPTD SSTARRIEEL QDSLQKKDAD LRAMEERYRR
610 620 630 640 650
YVDKARTVIQ TLEPKQRPPT VVSPEFHTLR SQLWERNLRI RQMEMDYEKS
660 670 680 690 700
RRRQEQEEKL LISAWYSMGM ALEHRAGEEH APAHAQSFLA QQRLATNARR
710
GPLGRQALSL RPTDKH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1B0GT78 | A0A1B0GT78_MOUSE | Protein Hook homolog 2 | Hook2 | 692 | Annotation score: | ||
A0A1B0GSU8 | A0A1B0GSU8_MOUSE | Protein Hook homolog 2 | Hook2 | 611 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 | N → D in AAH19486 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 158 | T → A in AAH19486 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 312 | A → T in AAH19486 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 312 | A → T in AAH26609 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 574 | Missing in AAH55881 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 694 | L → M in BAC31143 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK042048 mRNA Translation: BAC31143.1 AK145768 mRNA Translation: BAE26639.1 AK169073 mRNA Translation: BAE40859.1 BC002226 mRNA Translation: AAH02226.1 Different initiation. BC019486 mRNA Translation: AAH19486.1 BC026609 mRNA Translation: AAH26609.1 BC055881 mRNA Translation: AAH55881.1 BC080744 mRNA Translation: AAH80744.1 |
CCDSi | CCDS22489.1 |
RefSeqi | NP_001161463.1, NM_001167991.1 NP_573518.2, NM_133255.2 |
Genome annotation databases
Ensembli | ENSMUST00000064495; ENSMUSP00000067752; ENSMUSG00000052566 |
GeneIDi | 170833 |
KEGGi | mmu:170833 |
UCSCi | uc009moq.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK042048 mRNA Translation: BAC31143.1 AK145768 mRNA Translation: BAE26639.1 AK169073 mRNA Translation: BAE40859.1 BC002226 mRNA Translation: AAH02226.1 Different initiation. BC019486 mRNA Translation: AAH19486.1 BC026609 mRNA Translation: AAH26609.1 BC055881 mRNA Translation: AAH55881.1 BC080744 mRNA Translation: AAH80744.1 |
CCDSi | CCDS22489.1 |
RefSeqi | NP_001161463.1, NM_001167991.1 NP_573518.2, NM_133255.2 |
3D structure databases
SMRi | Q7TMK6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228463, 3 interactors |
IntActi | Q7TMK6, 3 interactors |
STRINGi | 10090.ENSMUSP00000067752 |
PTM databases
iPTMneti | Q7TMK6 |
PhosphoSitePlusi | Q7TMK6 |
Proteomic databases
EPDi | Q7TMK6 |
MaxQBi | Q7TMK6 |
PaxDbi | Q7TMK6 |
PRIDEi | Q7TMK6 |
Protocols and materials databases
Antibodypediai | 26165, 105 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000064495; ENSMUSP00000067752; ENSMUSG00000052566 |
GeneIDi | 170833 |
KEGGi | mmu:170833 |
UCSCi | uc009moq.2, mouse |
Organism-specific databases
CTDi | 29911 |
MGIi | MGI:2181664, Hook2 |
Phylogenomic databases
eggNOGi | ENOG502QTJ1, Eukaryota |
GeneTreei | ENSGT00940000160152 |
HOGENOMi | CLU_011214_1_0_1 |
InParanoidi | Q7TMK6 |
OMAi | MLCAQEE |
OrthoDBi | 398210at2759 |
TreeFami | TF320231 |
Miscellaneous databases
BioGRID-ORCSi | 170833, 2 hits in 17 CRISPR screens |
ChiTaRSi | Hook2, mouse |
PROi | PR:Q7TMK6 |
RNActi | Q7TMK6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000052566, Expressed in midgut and 217 other tissues |
ExpressionAtlasi | Q7TMK6, baseline and differential |
Genevisiblei | Q7TMK6, MM |
Family and domain databases
Gene3Di | 1.10.418.10, 1 hit |
InterProi | View protein in InterPro IPR001715, CH-domain IPR036872, CH_dom_sf IPR008636, Hook_C IPR043936, HOOK_N |
Pfami | View protein in Pfam PF05622, HOOK, 1 hit PF19047, HOOK_N, 1 hit |
PROSITEi | View protein in PROSITE PS50021, CH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HOOK2_MOUSE | |
Accessioni | Q7TMK6Primary (citable) accession number: Q7TMK6 Secondary accession number(s): Q66JV2 Q99LU2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 2, 2004 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 133 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families