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Protein

Protein Hook homolog 2

Gene

Hook2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Contributes to the establishment and maintenance of centrosome function. May function in the positioning or formation of aggresomes, which are pericentriolar accumulations of misfolded proteins, proteasomes and chaperones.By similarity

GO - Molecular functioni

  • dynein light intermediate chain binding Source: GO_Central
  • identical protein binding Source: MGI
  • microtubule binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Hook homolog 2
Short name:
mHK2
Gene namesi
Name:Hook2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2181664 Hook2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191951 – 716Protein Hook homolog 2Add BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei163PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7TMK6
MaxQBiQ7TMK6
PaxDbiQ7TMK6
PRIDEiQ7TMK6

PTM databases

iPTMnetiQ7TMK6
PhosphoSitePlusiQ7TMK6

Expressioni

Tissue specificityi

Expressed in brain, cerebellum, kidney, liver and heart, with highest levels in heart and kidney (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000052566 Expressed in 199 organ(s), highest expression level in midgut
CleanExiMM_HOOK2
ExpressionAtlasiQ7TMK6 baseline and differential
GenevisibleiQ7TMK6 MM

Interactioni

Subunit structurei

Self-associates (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3 (By similarity). May interact directly with AKTIP/FTS, HOOK1 and HOOK3 (By similarity). Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect (By similarity). Interacts with CNTRL (By similarity). Interacts with microtubules (By similarity). Interacts with ZC3H14 (By similarity). Interacts with LRGUK (via guanylate kinase-like domain) (PubMed:25781171, PubMed:28003339). Interacts with CCDC181 (PubMed:28283191).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228463, 1 interactor
IntActiQ7TMK6, 3 interactors
STRINGi10090.ENSMUSP00000067752

Structurei

3D structure databases

ProteinModelPortaliQ7TMK6
SMRiQ7TMK6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 122Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 546Sufficient for interaction with microtubulesBy similarityAdd BLAST546
Regioni1 – 161Required for localization to the centrosome and induction of aggresome formationBy similarityAdd BLAST161
Regioni533 – 716Required for localization to the centrosome and induction of aggresome formationBy similarityAdd BLAST184
Regioni582 – 716Sufficient for interaction with CNTRLBy similarityAdd BLAST135

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili188 – 427Sequence analysisAdd BLAST240
Coiled coili455 – 605Sequence analysisAdd BLAST151

Sequence similaritiesi

Belongs to the hook family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG1M Eukaryota
ENOG410XQ3G LUCA
GeneTreeiENSGT00690000101702
HOGENOMiHOG000294112
HOVERGENiHBG051920
InParanoidiQ7TMK6
KOiK16611
OMAiERNTVYM
OrthoDBiEOG091G06O9
TreeFamiTF320231

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR008636 Hook-like_fam
PfamiView protein in Pfam
PF05622 HOOK, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q7TMK6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVDKAELCG SLLTWLQTFQ VSPPCASPQD LSSGLAIAHV LNQIDPSWFN
60 70 80 90 100
NEWLQGISED SSPSWRLKVR KLEKILQSLV EYSKNVLGHP VSDQHLPDVS
110 120 130 140 150
LIGEFSNPAE LGKLLQLVLG CAISCEKKQE YIQRIMTLEE SVQHVVMEAI
160 170 180 190 200
QELMTKDTPD SLSPENYGNF DTQSRRYYFL SEEVEEGDHL QQHYLDLERQ
210 220 230 240 250
LLLLSEEKQN LAQENAALRE RVGRSEVESA PGLTAKKLLL LQSQLEQLQE
260 270 280 290 300
ENFRLESSRE DDRLRCLELE REVAELQQRN QALTSLSQEA QALKDEMDEL
310 320 330 340 350
RQSSERARQL EATLNSCRRR LGELQELRRQ VRQLEERNAG HAERTRQLEE
360 370 380 390 400
ELRRAGSLRA QLEAQRRQVQ ELQGQWQEEA MKAEKWLFEC RNLEEKCDLV
410 420 430 440 450
TKEKERLLTE RDSLREANEE LRCAQLQPRG LAQADLSLDP TPSGLENLAA
460 470 480 490 500
EILPAELRET LVRLQLENKR LCQQEAADRE RQEELQRHLE EANRARHGLE
510 520 530 540 550
AQQRLNQQQL SELRAQVEEL QKALQEQGGK TEDPTLLKRK LEDHLQKLHE
560 570 580 590 600
ADLELQRKRE YIEELEPPTD SSTARRIEEL QDSLQKKDAD LRAMEERYRR
610 620 630 640 650
YVDKARTVIQ TLEPKQRPPT VVSPEFHTLR SQLWERNLRI RQMEMDYEKS
660 670 680 690 700
RRRQEQEEKL LISAWYSMGM ALEHRAGEEH APAHAQSFLA QQRLATNARR
710
GPLGRQALSL RPTDKH
Length:716
Mass (Da):83,366
Last modified:July 27, 2011 - v3
Checksum:iF8056FDE58E83DC5
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1B0GSU8A0A1B0GSU8_MOUSE
Protein Hook homolog 2
Hook2
611Annotation score:
A0A1B0GT78A0A1B0GT78_MOUSE
Protein Hook homolog 2
Hook2
692Annotation score:

Sequence cautioni

The sequence AAH02226 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51N → D in AAH19486 (PubMed:15489334).Curated1
Sequence conflicti158T → A in AAH19486 (PubMed:15489334).Curated1
Sequence conflicti312A → T in AAH19486 (PubMed:15489334).Curated1
Sequence conflicti312A → T in AAH26609 (PubMed:15489334).Curated1
Sequence conflicti574Missing in AAH55881 (PubMed:15489334).Curated1
Sequence conflicti694L → M in BAC31143 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042048 mRNA Translation: BAC31143.1
AK145768 mRNA Translation: BAE26639.1
AK169073 mRNA Translation: BAE40859.1
BC002226 mRNA Translation: AAH02226.1 Different initiation.
BC019486 mRNA Translation: AAH19486.1
BC026609 mRNA Translation: AAH26609.1
BC055881 mRNA Translation: AAH55881.1
BC080744 mRNA Translation: AAH80744.1
CCDSiCCDS22489.1
RefSeqiNP_001161463.1, NM_001167991.1
NP_573518.2, NM_133255.2
UniGeneiMm.247775

Genome annotation databases

EnsembliENSMUST00000064495; ENSMUSP00000067752; ENSMUSG00000052566
GeneIDi170833
KEGGimmu:170833
UCSCiuc009moq.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042048 mRNA Translation: BAC31143.1
AK145768 mRNA Translation: BAE26639.1
AK169073 mRNA Translation: BAE40859.1
BC002226 mRNA Translation: AAH02226.1 Different initiation.
BC019486 mRNA Translation: AAH19486.1
BC026609 mRNA Translation: AAH26609.1
BC055881 mRNA Translation: AAH55881.1
BC080744 mRNA Translation: AAH80744.1
CCDSiCCDS22489.1
RefSeqiNP_001161463.1, NM_001167991.1
NP_573518.2, NM_133255.2
UniGeneiMm.247775

3D structure databases

ProteinModelPortaliQ7TMK6
SMRiQ7TMK6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228463, 1 interactor
IntActiQ7TMK6, 3 interactors
STRINGi10090.ENSMUSP00000067752

PTM databases

iPTMnetiQ7TMK6
PhosphoSitePlusiQ7TMK6

Proteomic databases

EPDiQ7TMK6
MaxQBiQ7TMK6
PaxDbiQ7TMK6
PRIDEiQ7TMK6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064495; ENSMUSP00000067752; ENSMUSG00000052566
GeneIDi170833
KEGGimmu:170833
UCSCiuc009moq.2 mouse

Organism-specific databases

CTDi29911
MGIiMGI:2181664 Hook2

Phylogenomic databases

eggNOGiENOG410IG1M Eukaryota
ENOG410XQ3G LUCA
GeneTreeiENSGT00690000101702
HOGENOMiHOG000294112
HOVERGENiHBG051920
InParanoidiQ7TMK6
KOiK16611
OMAiERNTVYM
OrthoDBiEOG091G06O9
TreeFamiTF320231

Miscellaneous databases

PROiPR:Q7TMK6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052566 Expressed in 199 organ(s), highest expression level in midgut
CleanExiMM_HOOK2
ExpressionAtlasiQ7TMK6 baseline and differential
GenevisibleiQ7TMK6 MM

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR008636 Hook-like_fam
PfamiView protein in Pfam
PF05622 HOOK, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHOOK2_MOUSE
AccessioniPrimary (citable) accession number: Q7TMK6
Secondary accession number(s): Q66JV2
, Q8BY47, Q8R347, Q8VCN4, Q99LU2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 120 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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