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Protein

E3 ubiquitin-protein ligase UHRF2

Gene

Uhrf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 SUMO-, but not ubiquitin-, protein ligase for ZNF131 (By similarity). E3 ubiquitin-protein ligase that is an intermolecular hub protein in the cell cycle network. Ubiquitinates cyclins, CCND1 and CCNE1, in an apparently phosphorylation-independent manner and induces G1 arrest. Also ubiquitinates PCNP leading to its degradation by the proteasome. Through cooperative DNA and histone binding, may contribute to a tighter epigenetic control of gene expression in differentiated cells.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri340 – 396PHD-typePROSITE-ProRule annotationAdd BLAST57
Zinc fingeri734 – 773RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • histone binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • SUMO transferase activity Source: MGI
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: HGNC

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Transferase
Biological processCell cycle, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3899300 SUMOylation of transcription cofactors
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase UHRF2 (EC:2.3.2.27)
Alternative name(s):
NIRF
Np95-like ring finger protein
Nuclear protein 97
Nuclear zinc finger protein Np97
RING-type E3 ubiquitin transferase UHRF2
Ubiquitin-like PHD and RING finger domain-containing protein 2
Ubiquitin-like-containing PHD and RING finger domains protein 2
Gene namesi
Name:Uhrf2
Synonyms:Nirf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1923718 Uhrf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi214Y → A: Abolishes in vitro binding to H3K9me3. Prevents enrichment at pericentric heterochromatin. 1 Publication1
Mutagenesisi217Y → A: Abolishes in vitro binding to 'Lys-10' methylated H3, H3K9me3. Prevents enrichment at pericentric heterochromatin. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561481 – 803E3 ubiquitin-protein ligase UHRF2Add BLAST803

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei668PhosphoserineCombined sources1
Disulfide bondi705InterchainBy similarity

Post-translational modificationi

May be autoubiquitinated; which may lead to proteasomal degradation.By similarity
Phosphorylated. Phosphorylation may be mediated by CDK2 (By similarity).By similarity
Autosumoylated.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ7TMI3
PaxDbiQ7TMI3
PeptideAtlasiQ7TMI3
PRIDEiQ7TMI3

PTM databases

iPTMnetiQ7TMI3
PhosphoSitePlusiQ7TMI3

Expressioni

Inductioni

Up-regulated during cell differentiation.1 Publication

Gene expression databases

BgeeiENSMUSG00000024817 Expressed in 253 organ(s), highest expression level in superior cervical ganglion
ExpressionAtlasiQ7TMI3 baseline and differential
GenevisibleiQ7TMI3 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked (By similarity). Binds methylated CpG containing oligonucleotides. Interacts with PCNP, HDAC1 and CDK2 (inactive form). Component of a complex at least composed of UHRF2, CDK2 and CCNE1. Interacts directly with CCNE1; the interaction ubiquitinates CCNE1 and appears independent of CCNE1 phosphorylation. Interacts with CCND1; the interaction ubiquitinates CCND1 and appears independent of CCND1 phosphorylation. Interacts with p53/TP53 and RB1 (By similarity). Interacts with H3; the interaction has a preference for the 'Lys-9' trimethylated form of H3 (H3K9me3). Interacts with UBE2I (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025739

Structurei

3D structure databases

ProteinModelPortaliQ7TMI3
SMRiQ7TMI3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 78Ubiquitin-likePROSITE-ProRule annotationAdd BLAST78
Domaini449 – 613YDGPROSITE-ProRule annotationAdd BLAST165

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 312Required for interaction with histone H3Add BLAST195
Regioni195 – 289Interaction with PCNPBy similarityAdd BLAST95
Regioni415 – 645Methyl-CpG binding and interaction with HDAC1By similarityAdd BLAST231

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri340 – 396PHD-typePROSITE-ProRule annotationAdd BLAST57
Zinc fingeri734 – 773RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IFAP Eukaryota
COG3440 LUCA
GeneTreeiENSGT00390000008296
HOGENOMiHOG000124662
HOVERGENiHBG059298
InParanoidiQ7TMI3
KOiK15713
OMAiGRRISKY
OrthoDBiEOG091G06BT
PhylomeDBiQ7TMI3
TreeFamiTF106434

Family and domain databases

Gene3Di2.30.280.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR015947 PUA-like_sf
IPR036987 SRA-YDG_sf
IPR003105 SRA_YDG
IPR021991 TTD_dom
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00628 PHD, 1 hit
PF02182 SAD_SRA, 1 hit
PF12148 TTD, 1 hit
PF00240 ubiquitin, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 1 hit
SM00184 RING, 2 hits
SM00466 SRA, 1 hit
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF57903 SSF57903, 1 hit
SSF88697 SSF88697, 1 hit
PROSITEiView protein in PROSITE
PS50053 UBIQUITIN_2, 1 hit
PS51015 YDG, 1 hit
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7TMI3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MWIQVRTIDG SQTRTIEDVS RKATIEELRE RVWALFDVRP ECQRLFYRGK
60 70 80 90 100
QLENGYTLFD YDVGLNDIIQ LLVRPDSSLP STSKQNDAQV KPSSHNPPKV
110 120 130 140 150
KKTARGGSSS QPSTSARTCL IDPGFGLYKV NELVDARDVG LGAWFEAHIH
160 170 180 190 200
SVTRASDGHS RGKTPLKNGS SYKRTNGNVN HNSKENTNKL DNVPSTSNSD
210 220 230 240 250
SVAADEDVIY HIEYDEYPES GILEMNVKDL RPRARTILKW NELNVGDVVM
260 270 280 290 300
VNYNVENPGK RGFWYDAEIT TLKTISRTKK EVRVKVFLGG SEGTLNDCRV
310 320 330 340 350
MSVDEIFKIE KPGAHPISFA DGKFLRKNDP ECDLCGGDPD KTCHMCSCHK
360 370 380 390 400
CGEKRDPNMQ LLCDECNMAY HIYCLSPPLD KVPEEEYWYC PSCKTDSSEV
410 420 430 440 450
VKAGERLKLS KKKAKMPSAS TESRRDWGRG MACVGRTKEC TIVPSNHYGP
460 470 480 490 500
IPGIPVGSTW RFRVQVSEAG VHRPHVGGIH GRSNDGAYSL VLAGGFEDEV
510 520 530 540 550
DRGDEFTYTG SGGKNLAGNK RIGAPSADQT LTNMNRALAL NCDAPLDDKI
560 570 580 590 600
GAESRNWRAG KPVRVIRSFK GRKISKYAPE EGNRYDGIYK VVKYWPEISS
610 620 630 640 650
SHGFLVWRYL LRRDDVEPAP WTSEGIERSR RLCLRLQYPA GYPSEKEGKK
660 670 680 690 700
TKGQSKKQGS EATKRPASDD ECPGDSKVLK ASDSTDAVEA FQLTPQQQRL
710 720 730 740 750
IREDCQNQKL WDEVLASLVE GPNFLKKLEQ SFMCVCCQEL VYQPVTTECF
760 770 780 790 800
HNVCKDCLQR SFKAQVFSCP ACRHDLGQNY VMVLNETLQT LLDLFFPGYS

KGR
Length:803
Mass (Da):90,106
Last modified:October 1, 2003 - v1
Checksum:iD799B0205E0E036E
GO
Isoform 2 (identifier: Q7TMI3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     501-516: DRGDEFTYTGSGGKNL → VSNDVTLFFRTNLDHF
     517-803: Missing.

Show »
Length:516
Mass (Da):57,761
Checksum:i3D6862E9D1E1DEDD
GO
Isoform 3 (identifier: Q7TMI3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-803: Missing.

Note: No experimental confirmation available.
Show »
Length:216
Mass (Da):24,138
Checksum:iC2C868076470EBCC
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3T995Q3T995_MOUSE
E3 ubiquitin-protein ligase UHRF2
Uhrf2
202Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti274T → S in AAM33798 (Ref. 1) Curated1
Sequence conflicti278T → S in AAM33798 (Ref. 1) Curated1
Sequence conflicti793D → E in AAM33798 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013876217 – 803Missing in isoform 3. 1 PublicationAdd BLAST587
Alternative sequenceiVSP_013877501 – 516DRGDE…GGKNL → VSNDVTLFFRTNLDHF in isoform 2. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_013878517 – 803Missing in isoform 2. 1 PublicationAdd BLAST287

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB116653 mRNA Translation: BAC81739.1
AF274047 mRNA Translation: AAM33798.1
AK031036 mRNA Translation: BAC27224.1
AK041564 mRNA Translation: BAC30987.1
AK042321 mRNA Translation: BAC31223.1
AK051743 mRNA Translation: BAC34750.1
AK080925 mRNA Translation: BAC38081.1
BC060241 mRNA Translation: AAH60241.1
CCDSiCCDS37955.1 [Q7TMI3-1]
RefSeqiNP_659122.2, NM_144873.2 [Q7TMI3-1]
UniGeneiMm.313364

Genome annotation databases

EnsembliENSMUST00000025739; ENSMUSP00000025739; ENSMUSG00000024817 [Q7TMI3-1]
GeneIDi109113
KEGGimmu:109113
UCSCiuc008hef.1 mouse [Q7TMI3-3]
uc008heh.1 mouse [Q7TMI3-2]
uc008hei.1 mouse [Q7TMI3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB116653 mRNA Translation: BAC81739.1
AF274047 mRNA Translation: AAM33798.1
AK031036 mRNA Translation: BAC27224.1
AK041564 mRNA Translation: BAC30987.1
AK042321 mRNA Translation: BAC31223.1
AK051743 mRNA Translation: BAC34750.1
AK080925 mRNA Translation: BAC38081.1
BC060241 mRNA Translation: AAH60241.1
CCDSiCCDS37955.1 [Q7TMI3-1]
RefSeqiNP_659122.2, NM_144873.2 [Q7TMI3-1]
UniGeneiMm.313364

3D structure databases

ProteinModelPortaliQ7TMI3
SMRiQ7TMI3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025739

PTM databases

iPTMnetiQ7TMI3
PhosphoSitePlusiQ7TMI3

Proteomic databases

EPDiQ7TMI3
PaxDbiQ7TMI3
PeptideAtlasiQ7TMI3
PRIDEiQ7TMI3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025739; ENSMUSP00000025739; ENSMUSG00000024817 [Q7TMI3-1]
GeneIDi109113
KEGGimmu:109113
UCSCiuc008hef.1 mouse [Q7TMI3-3]
uc008heh.1 mouse [Q7TMI3-2]
uc008hei.1 mouse [Q7TMI3-1]

Organism-specific databases

CTDi115426
MGIiMGI:1923718 Uhrf2

Phylogenomic databases

eggNOGiENOG410IFAP Eukaryota
COG3440 LUCA
GeneTreeiENSGT00390000008296
HOGENOMiHOG000124662
HOVERGENiHBG059298
InParanoidiQ7TMI3
KOiK15713
OMAiGRRISKY
OrthoDBiEOG091G06BT
PhylomeDBiQ7TMI3
TreeFamiTF106434

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-3899300 SUMOylation of transcription cofactors

Miscellaneous databases

ChiTaRSiUhrf2 mouse
PROiPR:Q7TMI3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024817 Expressed in 253 organ(s), highest expression level in superior cervical ganglion
ExpressionAtlasiQ7TMI3 baseline and differential
GenevisibleiQ7TMI3 MM

Family and domain databases

Gene3Di2.30.280.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR015947 PUA-like_sf
IPR036987 SRA-YDG_sf
IPR003105 SRA_YDG
IPR021991 TTD_dom
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00628 PHD, 1 hit
PF02182 SAD_SRA, 1 hit
PF12148 TTD, 1 hit
PF00240 ubiquitin, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 1 hit
SM00184 RING, 2 hits
SM00466 SRA, 1 hit
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF57903 SSF57903, 1 hit
SSF88697 SSF88697, 1 hit
PROSITEiView protein in PROSITE
PS50053 UBIQUITIN_2, 1 hit
PS51015 YDG, 1 hit
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUHRF2_MOUSE
AccessioniPrimary (citable) accession number: Q7TMI3
Secondary accession number(s): Q8BG56
, Q8BJP6, Q8BY30, Q8K1I5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: October 1, 2003
Last modified: November 7, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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