UniProtKB - Q7TMF5 (SPA12_MOUSE)
Protein
Serpin A12
Gene
Serpina12
Organism
Mus musculus (Mouse)
Status
Functioni
Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.2 Publications
Activity regulationi
Inhibition of KLK7 is enhanced by heparin.By similarity
GO - Molecular functioni
- serine-type endopeptidase inhibitor activity Source: GO_Central
GO - Biological processi
- negative regulation of endopeptidase activity Source: GO_Central
- negative regulation of gluconeogenesis Source: MGI
- negative regulation of lipid biosynthetic process Source: MGI
- positive regulation of insulin receptor signaling pathway Source: MGI
- positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
- regulation of cholesterol metabolic process Source: MGI
- regulation of triglyceride metabolic process Source: MGI
Keywordsi
Molecular function | Protease inhibitor, Serine protease inhibitor |
Protein family/group databases
MEROPSi | I04.091 |
Names & Taxonomyi
Protein namesi | Recommended name: Serpin A12Alternative name(s): Visceral adipose tissue-derived serine protease inhibitor Short name: Vaspin Visceral adipose-specific serpin |
Gene namesi | Name:Serpina12 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915304, Serpina12 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular space Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | 1 PublicationAdd BLAST | 20 | |
ChainiPRO_0000041977 | 21 – 413 | Serpin A12Add BLAST | 393 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 92 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 267 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Glycosylation slightly decreases affinity for heparin, but otherwise has no significant effect on KLK7 inhibitory activity or thermal stability of the protein.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 378 – 379 | CleavageBy similarity | 2 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q7TMF5 |
PRIDEi | Q7TMF5 |
PTM databases
GlyGeni | Q7TMF5, 2 sites |
PhosphoSitePlusi | Q7TMF5 |
Expressioni
Tissue specificityi
Expressed in visceral adipose tissues.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000041567, Expressed in tail skin and 82 other tissues |
Genevisiblei | Q7TMF5, MM |
Interactioni
Subunit structurei
Forms a stable complex with KLK7.
By similarityProtein-protein interaction databases
BioGRIDi | 212632, 7 interactors |
STRINGi | 10090.ENSMUSP00000045572 |
Miscellaneous databases
RNActi | Q7TMF5, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 364 – 382 | Reactive center loopBy similarityAdd BLAST | 19 |
Domaini
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.By similarity
Sequence similaritiesi
Belongs to the serpin family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2392, Eukaryota |
GeneTreei | ENSGT00940000161977 |
HOGENOMi | CLU_023330_2_1_1 |
InParanoidi | Q7TMF5 |
OMAi | VPMMFRG |
OrthoDBi | 1124079at2759 |
PhylomeDBi | Q7TMF5 |
TreeFami | TF343201 |
Family and domain databases
Gene3Di | 2.30.39.10, 1 hit 3.30.497.10, 1 hit |
InterProi | View protein in InterPro IPR023795, Serpin_CS IPR023796, Serpin_dom IPR000215, Serpin_fam IPR036186, Serpin_sf IPR042178, Serpin_sf_1 IPR042185, Serpin_sf_2 |
PANTHERi | PTHR11461, PTHR11461, 1 hit |
Pfami | View protein in Pfam PF00079, Serpin, 1 hit |
SMARTi | View protein in SMART SM00093, SERPIN, 1 hit |
SUPFAMi | SSF56574, SSF56574, 1 hit |
PROSITEi | View protein in PROSITE PS00284, SERPIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q7TMF5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTRMLDLGLF LAGLLTVKGL LQDRDAPDMY DSPVRVQEWR GKKDARQLAR
60 70 80 90 100
HNMEFGFKLL QRLASNSPQG NIFLSPLSIS TAFSMLSLGA QNSTLEEIRE
110 120 130 140 150
GFNFKEMSNW DVHAAFHYLL HKLNQETEDT KMNLGNALFM DQKLRPQQRF
160 170 180 190 200
LNLAKNVYDA DMVLTNFQDL ENTQKDINRY ISQKTHSRIK NMVKSIDPGT
210 220 230 240 250
VMILTNYIYF RGRWQYEFDP KQTKEEEFFI EKGKTVKVPM MFQRGLYDMA
260 270 280 290 300
YDSQLSCTIL EIPYRGNITA TFVLPDNGKL KLLEQGLQAD IFAKWKSLLS
310 320 330 340 350
KRVVDVWVPK LRISSTYNMK KVLSRLGISK IFEENGDLTR ISSHRSLKVG
360 370 380 390 400
EAVHKAELKM DEKGMEGAAG SGAQTLPMET PRHMKLDRPF LMMIYENFMP
410
SMVFLARIYD PSG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 69 | Q → R in AAP88383 (PubMed:16030142).Curated | 1 | |
Sequence conflicti | 110 | W → R in AAP88383 (PubMed:16030142).Curated | 1 | |
Sequence conflicti | 183 | Q → R in AAP88383 (PubMed:16030142).Curated | 1 | |
Sequence conflicti | 403 | V → I in AAP88383 (PubMed:16030142).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY326419 mRNA Translation: AAP88383.1 AK014346 mRNA Translation: BAB29287.1 AK014589 mRNA Translation: BAB29447.1 |
CCDSi | CCDS26145.1 |
RefSeqi | NP_080811.1, NM_026535.2 |
Genome annotation databases
Ensembli | ENSMUST00000043915; ENSMUSP00000045572; ENSMUSG00000041567 |
GeneIDi | 68054 |
KEGGi | mmu:68054 |
UCSCi | uc007owp.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY326419 mRNA Translation: AAP88383.1 AK014346 mRNA Translation: BAB29287.1 AK014589 mRNA Translation: BAB29447.1 |
CCDSi | CCDS26145.1 |
RefSeqi | NP_080811.1, NM_026535.2 |
3D structure databases
SMRi | Q7TMF5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 212632, 7 interactors |
STRINGi | 10090.ENSMUSP00000045572 |
Protein family/group databases
MEROPSi | I04.091 |
PTM databases
GlyGeni | Q7TMF5, 2 sites |
PhosphoSitePlusi | Q7TMF5 |
Proteomic databases
PaxDbi | Q7TMF5 |
PRIDEi | Q7TMF5 |
Protocols and materials databases
Antibodypediai | 27079, 360 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000043915; ENSMUSP00000045572; ENSMUSG00000041567 |
GeneIDi | 68054 |
KEGGi | mmu:68054 |
UCSCi | uc007owp.1, mouse |
Organism-specific databases
CTDi | 145264 |
MGIi | MGI:1915304, Serpina12 |
Phylogenomic databases
eggNOGi | KOG2392, Eukaryota |
GeneTreei | ENSGT00940000161977 |
HOGENOMi | CLU_023330_2_1_1 |
InParanoidi | Q7TMF5 |
OMAi | VPMMFRG |
OrthoDBi | 1124079at2759 |
PhylomeDBi | Q7TMF5 |
TreeFami | TF343201 |
Miscellaneous databases
BioGRID-ORCSi | 68054, 0 hits in 17 CRISPR screens |
ChiTaRSi | Serpina12, mouse |
PROi | PR:Q7TMF5 |
RNActi | Q7TMF5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041567, Expressed in tail skin and 82 other tissues |
Genevisiblei | Q7TMF5, MM |
Family and domain databases
Gene3Di | 2.30.39.10, 1 hit 3.30.497.10, 1 hit |
InterProi | View protein in InterPro IPR023795, Serpin_CS IPR023796, Serpin_dom IPR000215, Serpin_fam IPR036186, Serpin_sf IPR042178, Serpin_sf_1 IPR042185, Serpin_sf_2 |
PANTHERi | PTHR11461, PTHR11461, 1 hit |
Pfami | View protein in Pfam PF00079, Serpin, 1 hit |
SMARTi | View protein in SMART SM00093, SERPIN, 1 hit |
SUPFAMi | SSF56574, SSF56574, 1 hit |
PROSITEi | View protein in PROSITE PS00284, SERPIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPA12_MOUSE | |
Accessioni | Q7TMF5Primary (citable) accession number: Q7TMF5 Secondary accession number(s): Q9CQ32 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2005 |
Last sequence update: | September 27, 2005 | |
Last modified: | December 2, 2020 | |
This is version 114 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families