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Protein

Protein phosphatase 1 regulatory subunit 3C

Gene

Ppp1r3c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a glycogen-targeting subunit for PP1 and regulates its activity. Activates glycogen synthase, reduces glycogen phosphorylase activity and limits glycogen breakdown. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in a variety of cell types.10 Publications

GO - Molecular functioni

GO - Biological processi

  • glycogen biosynthetic process Source: MGI
  • glycogen metabolic process Source: MGI
  • protein targeting Source: MGI

Keywordsi

Biological processCarbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

ReactomeiR-MMU-3322077 Glycogen synthesis

Protein family/group databases

CAZyiCBM21 Carbohydrate-Binding Module Family 21

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 3C
Alternative name(s):
Protein phosphatase 1 regulatory subunit 5
Short name:
PP1 subunit R5
Protein targeting to glycogen
Short name:
PTG
Gene namesi
Name:Ppp1r3cImported
Synonyms:Ppp1r5By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1858229 Ppp1r3c

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Mice display embryonic lethality when homozygous. Heterozygotes display decreased protein levels, decreased glycogen accumulation and glycogen synthase activity, reduced insulin-stimulated glycogen synthesis and progressive age-dependent glucose intolerance. When Ppp1r3c is silenced in adults, they display decreased PP1 activity and glycogen accumulation, increased phosphorylation of glycogen phosphorylase, increased GLUT1 levels, increased glucose uptake and increased glycogen degradation.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi85V → A: Abolishes binding to PP1 but has no effect on binding to glycogen synthase, glycogen phosphorylase or glycogen; when associated with A-87. 1 Publication1
Mutagenesisi87F → A: Abolishes binding to PP1 but has no effect on binding to glycogen synthase, glycogen phosphorylase or glycogen; when associated with A-85. 1 Publication1
Mutagenesisi247D → A: Abolishes binding to glycogen synthase and glycogen phosphorylase but has no effect on binding to PP1 or glycogen; when associated with A-250. 1 Publication1
Mutagenesisi250E → A: Abolishes binding to glycogen synthase and glycogen phosphorylase but has no effect on binding to PP1 or glycogen; when associated with A-247. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002859281 – 317Protein phosphatase 1 regulatory subunit 3CAdd BLAST317

Post-translational modificationi

Ubiquitinated by NHLRC1/malin in a EPM2A/laforin-dependent manner.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ7TMB3
PaxDbiQ7TMB3
PRIDEiQ7TMB3

PTM databases

iPTMnetiQ7TMB3
PhosphoSitePlusiQ7TMB3

Expressioni

Gene expression databases

BgeeiENSMUSG00000067279 Expressed in 270 organ(s), highest expression level in muscle of leg
GenevisibleiQ7TMB3 MM

Interactioni

Subunit structurei

Interacts with PPP1CC catalytic subunit of PP1 and associates with glycogen. Forms complexes with glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity. Also interacts with EPM2A/laforin.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084578

Structurei

3D structure databases

ProteinModelPortaliQ7TMB3
SMRiQ7TMB3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini149 – 257CBM21PROSITE-ProRule annotationAdd BLAST109

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 263Interaction with EPM2ABy similarityAdd BLAST123

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi84 – 87PP1-binding motif4

Domaini

The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to glycogen phosphorylase, glycogen synthase and phosphorylase kinase.1 Publication

Phylogenomic databases

eggNOGiKOG3986 Eukaryota
ENOG4111FT1 LUCA
GeneTreeiENSGT00530000062978
HOGENOMiHOG000026799
HOVERGENiHBG052744
InParanoidiQ7TMB3
KOiK07189
OMAiTVKVKNM
OrthoDBiEOG091G0GB5
PhylomeDBiQ7TMB3
TreeFamiTF105537

Family and domain databases

Gene3Di2.60.40.2440, 1 hit
InterProiView protein in InterPro
IPR005036 CBM21_dom
IPR038175 CBM21_dom_sf
IPR017434 Pase-1_reg-su_3B/C/D_met
IPR030683 PP1_3C
PANTHERiPTHR12307:SF15 PTHR12307:SF15, 1 hit
PfamiView protein in Pfam
PF03370 CBM_21, 1 hit
PIRSFiPIRSF038207 PP1_GT_animal, 1 hit
PIRSF500813 PP1_PTG, 1 hit
PROSITEiView protein in PROSITE
PS51159 CBM21, 1 hit

Sequencei

Sequence statusi: Complete.

Q7TMB3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSCTRMIHVL DPRPLTSSVM PVDMAMRICL AHSPPLKSFL GPYNGFQRRN
60 70 80 90 100
FVNKLKPLKP CLSVKQEAKS QSEWKSPHNQ AKKRVVFADS KGLSLTAIHV
110 120 130 140 150
FSDLPEEPAW DLQFDLLDLN DISSSLKLHE EKNLVFDFPQ PSTDYLSFRD
160 170 180 190 200
RFQKNFVCLE NCSLQDRTVT GTVKVKNVSF EKKVQVRITF DTWKTYTDVD
210 220 230 240 250
CVYMKNVYSS SDSDTFSFAI DLPRVIPTEE KIEFCISYHA NGRIFWDNNE
260 270 280 290 300
GQNYRIVHVQ WKPDGVQTQV APKDCAFQQG PPKTEIEPTV FGSPRLASGL
310
FPEWQSWGRV ENLTSYR
Length:317
Mass (Da):36,460
Last modified:October 1, 2003 - v1
Checksum:i8DC8941B733649D6
GO

Sequence cautioni

The sequence AAB49689 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165Q → E in AAB49689 (PubMed:9045612).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC052769 mRNA Translation: AAH52769.1
BC054739 mRNA Translation: AAH54739.1
U89924 mRNA Translation: AAB49689.1 Different initiation.
AK078506 mRNA Translation: BAC37313.1
CCDSiCCDS37968.1
RefSeqiNP_058550.1, NM_016854.2
UniGeneiMm.24724

Genome annotation databases

EnsembliENSMUST00000087321; ENSMUSP00000084578; ENSMUSG00000067279
GeneIDi53412
KEGGimmu:53412
UCSCiuc008hhs.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC052769 mRNA Translation: AAH52769.1
BC054739 mRNA Translation: AAH54739.1
U89924 mRNA Translation: AAB49689.1 Different initiation.
AK078506 mRNA Translation: BAC37313.1
CCDSiCCDS37968.1
RefSeqiNP_058550.1, NM_016854.2
UniGeneiMm.24724

3D structure databases

ProteinModelPortaliQ7TMB3
SMRiQ7TMB3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084578

Protein family/group databases

CAZyiCBM21 Carbohydrate-Binding Module Family 21

PTM databases

iPTMnetiQ7TMB3
PhosphoSitePlusiQ7TMB3

Proteomic databases

MaxQBiQ7TMB3
PaxDbiQ7TMB3
PRIDEiQ7TMB3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087321; ENSMUSP00000084578; ENSMUSG00000067279
GeneIDi53412
KEGGimmu:53412
UCSCiuc008hhs.1 mouse

Organism-specific databases

CTDi5507
MGIiMGI:1858229 Ppp1r3c

Phylogenomic databases

eggNOGiKOG3986 Eukaryota
ENOG4111FT1 LUCA
GeneTreeiENSGT00530000062978
HOGENOMiHOG000026799
HOVERGENiHBG052744
InParanoidiQ7TMB3
KOiK07189
OMAiTVKVKNM
OrthoDBiEOG091G0GB5
PhylomeDBiQ7TMB3
TreeFamiTF105537

Enzyme and pathway databases

ReactomeiR-MMU-3322077 Glycogen synthesis

Miscellaneous databases

PROiPR:Q7TMB3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000067279 Expressed in 270 organ(s), highest expression level in muscle of leg
GenevisibleiQ7TMB3 MM

Family and domain databases

Gene3Di2.60.40.2440, 1 hit
InterProiView protein in InterPro
IPR005036 CBM21_dom
IPR038175 CBM21_dom_sf
IPR017434 Pase-1_reg-su_3B/C/D_met
IPR030683 PP1_3C
PANTHERiPTHR12307:SF15 PTHR12307:SF15, 1 hit
PfamiView protein in Pfam
PF03370 CBM_21, 1 hit
PIRSFiPIRSF038207 PP1_GT_animal, 1 hit
PIRSF500813 PP1_PTG, 1 hit
PROSITEiView protein in PROSITE
PS51159 CBM21, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPR3C_MOUSE
AccessioniPrimary (citable) accession number: Q7TMB3
Secondary accession number(s): O08541, Q8BJW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2003
Last modified: November 7, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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