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Protein

Choline transporter-like protein 4

Gene

slc44a4

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Choline transporter that seems to play a role in the choline-acetylcholine system and is required to the efferent innervation of hair cells in the olivocochlear bundle for the maintenance of physiological function of outer hair cells and the protection of hair cells from acoustic injury.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Choline transporter-like protein 4
Alternative name(s):
Solute carrier family 44 member 4
Gene namesi
Name:slc44a4
Synonyms:ctl4
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040426-1371 slc44a4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36CytoplasmicSequence analysisAdd BLAST36
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21
Topological domaini58 – 245ExtracellularSequence analysisAdd BLAST188
Transmembranei246 – 266HelicalSequence analysisAdd BLAST21
Topological domaini267 – 269CytoplasmicSequence analysis3
Transmembranei270 – 290HelicalSequence analysisAdd BLAST21
Topological domaini291 – 325ExtracellularSequence analysisAdd BLAST35
Transmembranei326 – 346HelicalSequence analysisAdd BLAST21
Topological domaini347 – 374CytoplasmicSequence analysisAdd BLAST28
Transmembranei375 – 395HelicalSequence analysisAdd BLAST21
Topological domaini396 – 464ExtracellularSequence analysisAdd BLAST69
Transmembranei465 – 485HelicalSequence analysisAdd BLAST21
Topological domaini486 – 516CytoplasmicSequence analysisAdd BLAST31
Transmembranei517 – 537HelicalSequence analysisAdd BLAST21
Topological domaini538 – 578ExtracellularSequence analysisAdd BLAST41
Transmembranei579 – 599HelicalSequence analysisAdd BLAST21
Topological domaini600 – 615CytoplasmicSequence analysisAdd BLAST16
Transmembranei616 – 636HelicalSequence analysisAdd BLAST21
Topological domaini637 – 655ExtracellularSequence analysisAdd BLAST19
Transmembranei656 – 676HelicalSequence analysisAdd BLAST21
Topological domaini677 – 723CytoplasmicSequence analysisAdd BLAST47

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Morpholino knockdown of the protein causes abnormal numbers of small, fused, and misplaced otoliths, and a smaller inner ear. Neuromasts of the morphants are smaller, and the number of lateral line neuromasts in the morphants is fewer than in the wild-type. Fewer hair cells per neuromast are found in both the anterior and posterior line of morpholino knockdown larvae (120 hpf) than in the wild-type. Knockdown larvae have a defective balance system, they show an abnormal swimming behavior by remaining stationary and resting in abnormal positions, they swim up and down and in circles. They also show some hearing loss.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003597221 – 723Choline transporter-like protein 4Add BLAST723

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi202N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi211N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi219N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi303N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi409N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi421N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi430N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Expressioni

Developmental stagei

Gene expression gradually increases from 36 to 96 h post-fertilization (hpf). At 12 hpf, expressed broadly, but at 26 hpf, the expression clusters around the otic vesicle, pectoral fin, and midbrain.1 Publication

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG062155
InParanoidiQ7T2B0
KOiK15377
PhylomeDBiQ7T2B0

Family and domain databases

InterProiView protein in InterPro
IPR007603 Choline_transptr-like
PANTHERiPTHR12385 PTHR12385, 1 hit
PfamiView protein in Pfam
PF04515 Choline_transpo, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q7T2B0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKKKQEEEQ NSSEYGAPAQ YDPTFNGPIH KRSCTDIICC VLFMLVITGY
60 70 80 90 100
MVVGILAWLY GDPRHVLYPR NSTGMFCGIG QNQNKPSVLY FDILKCATAT
110 120 130 140 150
NIMAAALQGL QCPTTQVCVS SCPSGFWALS PLAYLPNAKP ADYFQQELCV
160 170 180 190 200
PSLQLKDTTY TVMEIINKEL CPYYYTPTTS VLDRCLPSLG GSAYNPSNIP
210 220 230 240 250
ANFSLPGLSI NQTLSTIANA TSDLTNSFNM KDVGLRIFED FAKTWQWIVA
260 270 280 290 300
GLVIAMVVSV LFLLLLRFTA PVLIWILIFG VLAVGAFGIW YCYNDYMSLA
310 320 330 340 350
SSNLTFSNVG FTTNVQVYLQ VRDTWLAFLI ILCIVEAVLI LALIFLRTRI
360 370 380 390 400
LIAIALIQET SKALGHMMST LLYPVVTFVL LLVCVSYWGI TALYLATSGA
410 420 430 440 450
PIYKVVALNT TQGDCSNIQA NQTCDPQTFN SSRYSSCPSA RCVFINYNTE
460 470 480 490 500
GLFQRNLFNL QIYNVVAFLW CVNFVIALGH CTLAGAFASY YWAFSKPADI
510 520 530 540 550
PTFPLTQSFM RALRYHVGSL AFGALILTLV QIVRIILEYL DHKFKAAQNP
560 570 580 590 600
CARFLMCCLK CCFWCLEKFI KFINRNAYIM IAIYGKNFCV SAKNAFFLLM
610 620 630 640 650
RNIVRVVVLD KVTDLLLFFG KLLVVGGIGV LAFFFFSGRI QLPGNTFQTA
660 670 680 690 700
ALNYYWMPII TVVFGAYLIA HGFFSVYNMG VDTLFLCFLE DLERNDGSAE
710 720
KPYFMSKNLM KILNKKNKQP KTG
Length:723
Mass (Da):80,873
Last modified:October 1, 2003 - v1
Checksum:iFD835A6547F73455
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2KUI9A0A0G2KUI9_DANRE
Choline transporter-like protein 4
slc44a4
703Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC054620 mRNA Translation: AAH54620.1
RefSeqiNP_956707.1, NM_200413.1
UniGeneiDr.151382

Genome annotation databases

GeneIDi393385
KEGGidre:393385

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC054620 mRNA Translation: AAH54620.1
RefSeqiNP_956707.1, NM_200413.1
UniGeneiDr.151382

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi393385
KEGGidre:393385

Organism-specific databases

CTDi80736
ZFINiZDB-GENE-040426-1371 slc44a4

Phylogenomic databases

HOVERGENiHBG062155
InParanoidiQ7T2B0
KOiK15377
PhylomeDBiQ7T2B0

Miscellaneous databases

PROiPR:Q7T2B0

Family and domain databases

InterProiView protein in InterPro
IPR007603 Choline_transptr-like
PANTHERiPTHR12385 PTHR12385, 1 hit
PfamiView protein in Pfam
PF04515 Choline_transpo, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCTL4_DANRE
AccessioniPrimary (citable) accession number: Q7T2B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: October 1, 2003
Last modified: November 22, 2017
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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