UniProtKB - Q7SXN5 (DNM1L_DANRE)
Dynamin-1-like protein
dnm1l
Functioni
Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes. While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane (By similarity).
May play a role in the circadian control of mitochondrial ATP production (By similarity).
By similarityCatalytic activityi
- EC:3.6.5.5
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 32 – 40 | GTPBy similarity | 9 | |
Nucleotide bindingi | 214 – 220 | GTPBy similarity | 7 | |
Nucleotide bindingi | 245 – 248 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB-KW
- lipid binding Source: UniProtKB-KW
- microtubule binding Source: GO_Central
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- dynamin family protein polymerization involved in mitochondrial fission Source: UniProtKB
- mitochondrial fission Source: UniProtKB
- mitochondrial fragmentation involved in apoptotic process Source: GO_Central
- peroxisome fission Source: InterPro
- protein complex oligomerization Source: UniProtKB
- rhythmic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Biological rhythms |
Ligand | GTP-binding, Lipid-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DRE-75153, Apoptotic execution phase |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dnm1l ORF Names:zgc:66163 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-040426-1556, dnm1l |
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Cytoplasm and Cytosol
- cytosol By similarity
Golgi apparatus
- Golgi apparatus By similarity
Mitochondrion
- Mitochondrion outer membrane By similarity; Peripheral membrane protein By similarity
Other locations
- Endomembrane system By similarity; Peripheral membrane protein By similarity
- clathrin-coated pit By similarity
- synaptic vesicle membrane By similarity
Cytoskeleton
- microtubule Source: GO_Central
Cytosol
- cytosol Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial outer membrane Source: UniProtKB
- mitochondrion Source: UniProtKB
Peroxisome
- peroxisome Source: UniProtKB
Plasma Membrane
- clathrin-coated pit Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
- membrane Source: GO_Central
- synaptic vesicle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Coated pit, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206569 | 1 – 691 | Dynamin-1-like proteinAdd BLAST | 691 |
Proteomic databases
PaxDbi | Q7SXN5 |
Expressioni
Gene expression databases
Bgeei | ENSDARG00000015006, Expressed in retina and 36 other tissues |
ExpressionAtlasi | Q7SXN5, baseline and differential |
Interactioni
Subunit structurei
Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Oligomerizes in a GTP-dependent manner to form membrane-associated tubules with a spiral pattern.
By similarityGO - Molecular functioni
- microtubule binding Source: GO_Central
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000006315 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 301 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 280 | |
Domaini | 599 – 690 | GEDPROSITE-ProRule annotationAdd BLAST | 92 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 32 – 39 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 58 – 60 | G2 motifPROSITE-ProRule annotation | 3 | |
Regioni | 145 – 148 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 214 – 217 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 244 – 247 | G5 motifPROSITE-ProRule annotation | 4 | |
Regioni | 343 – 488 | Middle domainBy similarityAdd BLAST | 146 | |
Regioni | 522 – 573 | DisorderedSequence analysisAdd BLAST | 52 | |
Regioni | 609 – 623 | Important for homodimerizationBy similarityAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0446, Eukaryota |
GeneTreei | ENSGT00940000155504 |
HOGENOMi | CLU_008964_5_0_1 |
InParanoidi | Q7SXN5 |
OMAi | NIMATQF |
OrthoDBi | 264244at2759 |
PhylomeDBi | Q7SXN5 |
TreeFami | TF352031 |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR030556, DNM1L IPR022812, Dynamin IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR045063, Dynamin_N IPR000375, Dynamin_stalk IPR030381, G_DYNAMIN_dom IPR003130, GED IPR020850, GED_dom IPR027417, P-loop_NTPase |
PANTHERi | PTHR11566, PTHR11566, 2 hits PTHR11566:SF39, PTHR11566:SF39, 2 hits |
Pfami | View protein in Pfam PF01031, Dynamin_M, 1 hit PF00350, Dynamin_N, 1 hit PF02212, GED, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit SM00302, GED, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MEALIPVINK LQDVFNTVGA DIIQLPQIAV VGTQSSGKSS VLESLVGRDL
60 70 80 90 100
LPRGTGIVTR RPLILQLVHV DPEDRRKTSE ENGVDGEEWG KFLHTKNKIY
110 120 130 140 150
TDFDEIRQEI ENETERVSGN NKGISDEPIH LKIFSPHVVN LTLVDLPGIT
160 170 180 190 200
KVPVGDQPKD IELQIRELIL KYISNPNSII LAVTAANTDM ATSEALKVAR
210 220 230 240 250
EVDPDGRRTL AVVTKLDLMD AGTDAMDVLM GRVIPVKLGL IGVVNRSQLD
260 270 280 290 300
INNKKSVADS IRDEHGFLQK KYPSLANRNG TKYLARTLNR LLMHHIRDCL
310 320 330 340 350
PELKTRINVL SAQYQSLLSS YGEPVEDMSA TLLQLITKFA TEYCNTIEGT
360 370 380 390 400
AKYIETAELC GGARICYIFH ETFGRTLESV DPLGGLTTID VLTAIRNATG
410 420 430 440 450
PRPALFVPEV SFELLVKRQV KRLEEPSLRC VELVHEEMQR IIQHCSNYST
460 470 480 490 500
QELLRFPKLH DAIVEVVTSL LRKRLPVTNE MVHNLVAIEL AYINTKHPDF
510 520 530 540 550
ADACGLMNNN IEEQRRNRMR ELPTSVPRDK MAGGAQAEQE GGTGTWRGML
560 570 580 590 600
KKGDEGQGEE KTKLQSSIPA SPQKGHAVNL LDVPVPVARK LSAREQRDCE
610 620 630 640 650
VIERLIKSYF LIVRKNIQDS VPKAVMHFLV NHVKDSLQSE LVGQLYKPAL
660 670 680 690
LDDLLTESED MAQRRNEAAD MLKALQKASQ VIAEIRETHL W
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A140LH30 | A0A140LH30_DANRE | Dynamin-1-like protein | dnm1l | 715 | Annotation score: | ||
F1QB59 | F1QB59_DANRE | Dynamin GTPase | dnm1l | 94 | Annotation score: | ||
F1Q917 | F1Q917_DANRE | Dynamin GTPase | dnm1l | 228 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC055521 mRNA Translation: AAH55521.1 |
RefSeqi | NP_957216.1, NM_200922.1 |
Genome annotation databases
Ensembli | ENSDART00000006840; ENSDARP00000006315; ENSDARG00000015006 |
GeneIDi | 393896 |
KEGGi | dre:393896 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC055521 mRNA Translation: AAH55521.1 |
RefSeqi | NP_957216.1, NM_200922.1 |
3D structure databases
SMRi | Q7SXN5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000006315 |
Proteomic databases
PaxDbi | Q7SXN5 |
Genome annotation databases
Ensembli | ENSDART00000006840; ENSDARP00000006315; ENSDARG00000015006 |
GeneIDi | 393896 |
KEGGi | dre:393896 |
Organism-specific databases
CTDi | 10059 |
ZFINi | ZDB-GENE-040426-1556, dnm1l |
Phylogenomic databases
eggNOGi | KOG0446, Eukaryota |
GeneTreei | ENSGT00940000155504 |
HOGENOMi | CLU_008964_5_0_1 |
InParanoidi | Q7SXN5 |
OMAi | NIMATQF |
OrthoDBi | 264244at2759 |
PhylomeDBi | Q7SXN5 |
TreeFami | TF352031 |
Enzyme and pathway databases
Reactomei | R-DRE-75153, Apoptotic execution phase |
Miscellaneous databases
PROi | PR:Q7SXN5 |
Gene expression databases
Bgeei | ENSDARG00000015006, Expressed in retina and 36 other tissues |
ExpressionAtlasi | Q7SXN5, baseline and differential |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR030556, DNM1L IPR022812, Dynamin IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR045063, Dynamin_N IPR000375, Dynamin_stalk IPR030381, G_DYNAMIN_dom IPR003130, GED IPR020850, GED_dom IPR027417, P-loop_NTPase |
PANTHERi | PTHR11566, PTHR11566, 2 hits PTHR11566:SF39, PTHR11566:SF39, 2 hits |
Pfami | View protein in Pfam PF01031, Dynamin_M, 1 hit PF00350, Dynamin_N, 1 hit PF02212, GED, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit SM00302, GED, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DNM1L_DANRE | |
Accessioni | Q7SXN5Primary (citable) accession number: Q7SXN5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | October 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families