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UniProtKB - Q7SIG7 (DPOL_DESST)
Protein
DNA polymerase
Gene
pol
Organism
Desulfurococcus sp. (strain Tok)
Status
Functioni
Thermostable DNA polymerase. In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.
1 PublicationCaution
There are conflicts between the sequence shown here and that from PDB 1D5A.Curated
Catalytic activityi
- EC:2.7.7.71 Publication
Cofactori
Mg2+1 PublicationNote: Binds 2 magnesium ions.1 Publication
Activity regulationi
DNA polymerase activity strongly inhibited by uracil-containing oligonucleotides.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 141 | Magnesium 11 Publication | 1 | |
Metal bindingi | 141 | Magnesium 21 Publication | 1 | |
Metal bindingi | 143 | Magnesium 21 Publication | 1 | |
Metal bindingi | 315 | Magnesium 11 Publication | 1 | |
Metal bindingi | 315 | Magnesium 21 Publication | 1 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
- exonuclease activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- nucleotide binding Source: InterPro
GO - Biological processi
- DNA replication Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Multifunctional enzyme, Nuclease, Nucleotidyltransferase, Transferase |
Biological process | DNA replication |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 1918 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pol |
Organismi | Desulfurococcus sp. (strain Tok) |
Taxonomic identifieri | 108142 [NCBI] |
Taxonomic lineagei | Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Desulfurococcus › unclassified Desulfurococcus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000278146 | 1 – 773 | DNA polymeraseAdd BLAST | 773 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 428 ↔ 442 | 1 Publication | ||
Disulfide bondi | 506 ↔ 509 | 1 Publication |
Keywords - PTMi
Disulfide bondStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q7SIG7 |
SMRi | Q7SIG7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q7SIG7 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 131 | N-terminal domain1 PublicationAdd BLAST | 131 | |
Regioni | 133 – 385 | Exonuclease domain1 PublicationAdd BLAST | 253 | |
Regioni | 390 – 773 | Polymerase domain1 PublicationAdd BLAST | 384 |
Sequence similaritiesi
Belongs to the DNA polymerase type-B family.Sequence analysis
Family and domain databases
Gene3Di | 1.10.132.60, 1 hit 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR042087, DNA_pol_B_thumb IPR023211, DNA_pol_palm_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 2 hits |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
i Sequence
Sequence statusi: Complete.
Q7SIG7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MILDADYITE DGKPVIRVFK KEKGEFKIDY DRDFEPYIYA LLKDDSAIED
60 70 80 90 100
IKKITAERHG TTVRVTRAER VKKKFLGRPV EVWKLYFTHP QDVPAIRDKI
110 120 130 140 150
REHPAVVDIY EYDIPFAKRY LIDRGLIPME GDEELRMLAF DIETLYHEGE
160 170 180 190 200
EFGEGPILMI SYADEEGARV ITWKNIDLPY VESVSTEKEM IKRFLKVIQE
210 220 230 240 250
KDPDVLITYN GDNFDFAYLK KRSEMLGVKF ILGRDGSEPK IQRMGDRFAV
260 270 280 290 300
EVKGRIHFDL YPVIRRTINL PTYTLETVYE PVFGQPKEKV YAEEIARAWE
310 320 330 340 350
SGEGLERVAR YSMEDAKATY ELGKEFFPME AQLSRLVGQS LWDVSRSSTG
360 370 380 390 400
NLVEWFLLRK AYERNDVAPN KPDERELARR TESYAGGYVK EPEKGLWENI
410 420 430 440 450
VYLDYKSLYP SIIITHNVSP DTLNREGCRE YDVAPQVGHR FCKDFPGFIP
460 470 480 490 500
SLLGDLLEER QKVKKKMKAT VDPIERKLLD YRQRAIKILA NSYYGYYAYA
510 520 530 540 550
NARWYCRECA ESVTAWGRQY IETTMREIEE KFGFKVLYAD TDGFFATIPG
560 570 580 590 600
ADAETVKNKA KEFLNYINPR LPGLLELEYE GFYRRGFFVT KKKYAVIDEE
610 620 630 640 650
DKITTRGLEI VRRDWSEIAK ETQARVLEAI LKHGDVEEAV RIVKEVTEKL
660 670 680 690 700
SRHEVPPEKL VIYEQITRDL RSYRATGPHV AVAKRLAARG IKIRPGTVIS
710 720 730 740 750
YIVLKGPGRV GDRAIPFDEF DPAKHRYDAE YYIENQVLPA VERILRAFGY
760 770
RKEDLRYQKT KQAGLGAWLK PKT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 146 – 154 | YHEGEEFGE → AHAGAAAGA (PubMed:10545321).Curated | 9 | |
Sequence conflicti | 287 | K → A (PubMed:10545321).Curated | 1 | |
Sequence conflicti | 297 | R → E (PubMed:10545321).Curated | 1 | |
Sequence conflicti | 300 | E → A (PubMed:10545321).Curated | 1 | |
Sequence conflicti | 665 – 676 | QITRD…SYRAT → A (PubMed:10545321).CuratedAdd BLAST | 12 | |
Sequence conflicti | 682 – 696 | VAKRL…KIRPG → AAA (PubMed:10545321).CuratedAdd BLAST | 15 |
Similar proteinsi
Cross-referencesi
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1D5A | X-ray | 2.40 | A | 1-756 | [»] | |
1QQC | X-ray | 2.60 | A | 1-773 | [»] | |
AlphaFoldDBi | Q7SIG7 | |||||
SMRi | Q7SIG7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 1918 |
Miscellaneous databases
EvolutionaryTracei | Q7SIG7 |
Family and domain databases
Gene3Di | 1.10.132.60, 1 hit 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR042087, DNA_pol_B_thumb IPR023211, DNA_pol_palm_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 2 hits |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOL_DESST | |
Accessioni | Q7SIG7Primary (citable) accession number: Q7SIG7 Secondary accession number(s): Q7SIG8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | December 15, 2003 | |
Last modified: | May 25, 2022 | |
This is version 86 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families