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Protein

Cobalt-containing nitrile hydratase subunit beta

Gene
N/A
Organism
Pseudonocardia thermophila
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NHase catalyzes the hydration of various nitrile compounds to the corresponding amides.Curated

Catalytic activityi

An aliphatic amide = a nitrile + H2O.Curated1 Publication

Temperature dependencei

Optimum temperature is 60 degrees Celsius.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase

Names & Taxonomyi

Protein namesi
Recommended name:
Cobalt-containing nitrile hydratase subunit beta (EC:4.2.1.84)
Short name:
L-NHase
Short name:
L-nitrilase
OrganismiPseudonocardia thermophilaImported
Taxonomic identifieri1848 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaePseudonocardia

Pathology & Biotechi

Biotechnological usei

Industrial production of acrylamide is now being developed using some of these enzymes.Curated

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001868301 – 233Cobalt-containing nitrile hydratase subunit betaAdd BLAST233

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta chains.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Q7SID25EBI-1032285,EBI-1032292

Protein-protein interaction databases

IntActiQ7SID3, 1 interactor

Structurei

Secondary structure

1233
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ7SID3
SMRiQ7SID3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SID3

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiView protein in InterPro
IPR024690 CN_hydtase_beta_dom
IPR008990 Elect_transpt_acc-like_dom_sf
IPR003168 Nitrile_hydratase_bsu
PfamiView protein in Pfam
PF02211 NHase_beta, 1 hit
PIRSFiPIRSF001427 NHase_beta, 1 hit
SUPFAMiSSF50090 SSF50090, 1 hit
TIGRFAMsiTIGR03888 nitrile_beta, 1 hit

Sequencei

Sequence statusi: Complete.

Q7SID3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNGVYDVGGT DGLGPINRPA DEPVFRAEWE KVAFAMFPAT FRAGFMGLDE
60 70 80 90 100
FRFGIEQMNP AEYLESPYYW HWIRTYIHHG VRTGKIDLEE LERRTQYYRE
110 120 130 140 150
NPDAPLPEHE QKPELIEFVN QAVYGGLPAS REVDRPPKFK EGDVVRFSTA
160 170 180 190 200
SPKGHARRAR YVRGKTGTVV KHHGAYIYPD TAGNGLGECP EHLYTVRFTA
210 220 230
QELWGPEGDP NSSVYYDCWE PYIELVDTKA AAA
Length:233
Mass (Da):26,550
Last modified:March 1, 2004 - v2
Checksum:iECA070F18BCE62F0
GO

Sequence databases

RefSeqiWP_073455623.1, NZ_FRAP01000003.1

Similar proteinsi

Cross-referencesi

Sequence databases

RefSeqiWP_073455623.1, NZ_FRAP01000003.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IREX-ray1.80B1-228[»]
1UGPX-ray1.63B1-226[»]
1UGQX-ray2.00B1-228[»]
1UGRX-ray1.80B1-228[»]
1UGSX-ray2.00B1-228[»]
3VYHX-ray1.63B1-233[»]
4OB0X-ray1.20B1-233[»]
4OB1X-ray1.63B1-233[»]
4OB2X-ray1.52B1-233[»]
4OB3X-ray1.92B1-233[»]
ProteinModelPortaliQ7SID3
SMRiQ7SID3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7SID3, 1 interactor

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SID3

Family and domain databases

InterProiView protein in InterPro
IPR024690 CN_hydtase_beta_dom
IPR008990 Elect_transpt_acc-like_dom_sf
IPR003168 Nitrile_hydratase_bsu
PfamiView protein in Pfam
PF02211 NHase_beta, 1 hit
PIRSFiPIRSF001427 NHase_beta, 1 hit
SUPFAMiSSF50090 SSF50090, 1 hit
TIGRFAMsiTIGR03888 nitrile_beta, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNHAB_PSETH
AccessioniPrimary (citable) accession number: Q7SID3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: November 22, 2017
This is version 68 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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