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UniProtKB - Q7SIB3 (CO4A2_BOVIN)
Protein
Collagen alpha-2(IV) chain
Gene
COL4A2
Organism
Bos taurus (Bovine)
Status
Functioni
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Potently inhibits angiogenesis and tumor growth (By similarity).
By similarityGO - Molecular functioni
- extracellular matrix structural constituent Source: InterPro
Names & Taxonomyi
Protein namesi | Recommended name: Collagen alpha-2(IV) chain |
Gene namesi | Name:COL4A2 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-KW
Other locations
- basement membrane Source: UniProtKB-SubCell
- collagen trimer Source: UniProtKB-KW
Keywords - Cellular componenti
Basement membrane, Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059411 | ‹1 – 227 | Collagen alpha-2(IV) chainAdd BLAST | ›227 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 19 ↔ 108 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 52 ↔ 105 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 64 ↔ 70 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 127 ↔ 223 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 161 ↔ 220 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 173 ↔ 180 | PROSITE-ProRule annotation1 Publication |
Post-translational modificationi
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.By similarity
Keywords - PTMi
Disulfide bond, HydroxylationProteomic databases
PaxDbi | Q7SIB3 |
PTM databases
GlyConnecti | 109, 20 N-Linked glycans |
Interactioni
Subunit structurei
There are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network.
Interacts with EFEMP2 (By similarity).
By similarityProtein-protein interaction databases
ComplexPortali | CPX-3107, Collagen type IV trimer variant 1 |
STRINGi | 9913.ENSBTAP00000005916 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q7SIB3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q7SIB3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 227 | Collagen IV NC1PROSITE-ProRule annotationAdd BLAST | 224 |
Domaini
Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
Sequence similaritiesi
Belongs to the type IV collagen family.PROSITE-ProRule annotation
Keywords - Domaini
Collagen, RepeatPhylogenomic databases
eggNOGi | KOG3544, Eukaryota |
InParanoidi | Q7SIB3 |
OrthoDBi | 63831at2759 |
Family and domain databases
Gene3Di | 2.170.240.10, 1 hit |
InterProi | View protein in InterPro IPR001442, Collagen_IV_NC IPR036954, Collagen_IV_NC_sf IPR016187, CTDL_fold |
Pfami | View protein in Pfam PF01413, C4, 2 hits |
SMARTi | View protein in SMART SM00111, C4, 2 hits |
SUPFAMi | SSF56436, SSF56436, 2 hits |
PROSITEi | View protein in PROSITE PS51403, NC1_IV, 1 hit |
i Sequence
Sequence statusi: Fragment.
Q7SIB3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
ISIGYLLVKH SQTDQEPMCP VGMNKLWSGY SLLYFEGQEK AHNQDLGLAG
60 70 80 90 100
SCLARFSTMP FLYCNPGDVC YYASRNDKSY WLSTTAPLPM MPVAEEDIRP
110 120 130 140 150
YISRCSVCEA PAVAIAVHSQ DVSIPHCPAG WRSLWIGYSF LMHTAAGDEG
160 170 180 190 200
GGQSLVSPGS CLEDFRATPF IECNGARGTC HYYANKYSFW LTTIPEQSFQ
210 220
GTPSADTLKA GLIRTHISRC QVCMKNL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Similar proteinsi
Cross-referencesi
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1M3D | X-ray | 2.00 | C/F/I/L | 1-227 | [»] | |
1T60 | X-ray | 1.50 | C/F/I/L/O/R/U/X | 1-227 | [»] | |
1T61 | X-ray | 1.50 | C/F | 1-227 | [»] | |
SMRi | Q7SIB3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-3107, Collagen type IV trimer variant 1 |
STRINGi | 9913.ENSBTAP00000005916 |
PTM databases
GlyConnecti | 109, 20 N-Linked glycans |
Proteomic databases
PaxDbi | Q7SIB3 |
Phylogenomic databases
eggNOGi | KOG3544, Eukaryota |
InParanoidi | Q7SIB3 |
OrthoDBi | 63831at2759 |
Miscellaneous databases
EvolutionaryTracei | Q7SIB3 |
Family and domain databases
Gene3Di | 2.170.240.10, 1 hit |
InterProi | View protein in InterPro IPR001442, Collagen_IV_NC IPR036954, Collagen_IV_NC_sf IPR016187, CTDL_fold |
Pfami | View protein in Pfam PF01413, C4, 2 hits |
SMARTi | View protein in SMART SM00111, C4, 2 hits |
SUPFAMi | SSF56436, SSF56436, 2 hits |
PROSITEi | View protein in PROSITE PS51403, NC1_IV, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CO4A2_BOVIN | |
Accessioni | Q7SIB3Primary (citable) accession number: Q7SIB3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2005 |
Last sequence update: | December 15, 2003 | |
Last modified: | February 23, 2022 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families