UniProtKB - Q7SIB1 (CCA_GEOSE)
Protein
CCA-adding enzyme
Gene
cca
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Functioni
Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.
Miscellaneous
A single active site specifically recognizes both ATP and CTP and is responsible for their addition.
Catalytic activityi
- EC:2.7.7.72
Cofactori
Mg2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 27 | ATP or CTP; via amide nitrogen | 1 | |
Binding sitei | 30 | ATP or CTP | 1 | |
Metal bindingi | 40 | MagnesiumBy similarity | 1 | |
Metal bindingi | 42 | MagnesiumBy similarity | 1 | |
Binding sitei | 111 | ATP or CTP | 1 | |
Sitei | 112 | May assist in discriminating ATP from CTP | 1 | |
Sitei | 153 | Involved in nucleotide selectionSequence analysis | 1 | |
Binding sitei | 154 | ATP or CTP | 1 | |
Binding sitei | 157 | ATP or CTP | 1 | |
Binding sitei | 160 | ATP or CTP | 1 | |
Binding sitei | 163 | ATP or CTP | 1 |
GO - Molecular functioni
- ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-UniRule
- CTP:3'-cytidine-tRNA cytidylyltransferase activity Source: UniProtKB-EC
- CTP:tRNA cytidylyltransferase activity Source: UniProtKB-EC
- magnesium ion binding Source: UniProtKB-UniRule
- tRNA binding Source: UniProtKB-UniRule
- tRNA cytidylyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- RNA repair Source: UniProtKB-KW
- tRNA 3'-terminal CCA addition Source: UniProtKB-UniRule
Keywordsi
Molecular function | Nucleotidyltransferase, RNA-binding, Transferase |
Biological process | RNA repair, tRNA processing |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: CCA-adding enzyme (EC:2.7.7.72)Alternative name(s): CCA tRNA nucleotidyltransferase tRNA CCA-pyrophosphorylase tRNA adenylyl-/cytidylyl- transferase tRNA nucleotidyltransferase tRNA-NT |
Gene namesi | Name:cca |
Organismi | Geobacillus stearothermophilus (Bacillus stearothermophilus) |
Taxonomic identifieri | 1422 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000139033 | 1 – 404 | CCA-adding enzymeAdd BLAST | 404 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q7SIB1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q7SIB1 |
Family & Domainsi
Domaini
The crystal structure reveals a seahorse-shaped subunit consisting of four domains: head, neck, body, and tail. The head domain contains the nucleotidyltransferase activity. The neck domain provides a specific template for the incoming ATP or CTP, whereas the body and tail domains may be involved in tRNA binding.
Sequence similaritiesi
Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.Curated
Family and domain databases
CDDi | cd05398, NT_ClassII-CCAase, 1 hit |
Gene3Di | 3.30.460.10, 1 hit |
HAMAPi | MF_01263, CCA_bact_type3, 1 hit |
InterProi | View protein in InterPro IPR032810, CCA-adding_enz_C IPR023068, CCA-adding_enz_firmicutes IPR043519, NT_sf IPR002646, PolA_pol_head_dom IPR032828, PolyA_RNA-bd |
Pfami | View protein in Pfam PF01743, PolyA_pol, 1 hit PF12627, PolyA_pol_RNAbd, 1 hit PF13735, tRNA_NucTran2_2, 1 hit |
SUPFAMi | SSF81301, SSF81301, 1 hit |
i Sequence
Sequence statusi: Complete.
Q7SIB1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKPPFQEALG IIQQLKQHGY DAYFVGGAVR DLLLGRPIGD VDIATSALPE
60 70 80 90 100
DVMAIFPKTI DVGSKHGTVV VVHKGKAYEV TTFKTDGDYE DYRRPESVTF
110 120 130 140 150
VRSLEEDLKR RDFTMNAIAM DEYGTIIDPF GGREAIRRRI IRTVGEAEKR
160 170 180 190 200
FREDALRMMR AVRFVSELGF ALAPDTEQAI VQNAPLLAHI SVERMTMEME
210 220 230 240 250
KLLGGPFAAR ALPLLAETGL NAYLPGLAGK EKQLRLAAAY RWPWLAAREE
260 270 280 290 300
RWALLCHALG VQESRPFLRA WKLPNKVVDE AGAILTALAD IPRPEAWTNE
310 320 330 340 350
QLFSAGLERA LSVETVRAAF TGAPPGPWHE KLRRRFASLP IKTKGELAVN
360 370 380 390 400
GKDVIEWVGK PAGPWVKEAL DAIWRAVVNG EVENEKERIY AWLMERNRTR
EKNC
Similar proteinsi
Cross-referencesi
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MIV | X-ray | 3.50 | A/B | 1-404 | [»] | |
1MIW | X-ray | 3.00 | A/B | 1-404 | [»] | |
1MIY | X-ray | 3.52 | A/B | 1-404 | [»] | |
SMRi | Q7SIB1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
DrugBanki | DB02431, Cytidine-5'-Triphosphate |
Miscellaneous databases
EvolutionaryTracei | Q7SIB1 |
Family and domain databases
CDDi | cd05398, NT_ClassII-CCAase, 1 hit |
Gene3Di | 3.30.460.10, 1 hit |
HAMAPi | MF_01263, CCA_bact_type3, 1 hit |
InterProi | View protein in InterPro IPR032810, CCA-adding_enz_C IPR023068, CCA-adding_enz_firmicutes IPR043519, NT_sf IPR002646, PolA_pol_head_dom IPR032828, PolyA_RNA-bd |
Pfami | View protein in Pfam PF01743, PolyA_pol, 1 hit PF12627, PolyA_pol_RNAbd, 1 hit PF13735, tRNA_NucTran2_2, 1 hit |
SUPFAMi | SSF81301, SSF81301, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CCA_GEOSE | |
Accessioni | Q7SIB1Primary (citable) accession number: Q7SIB1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2005 |
Last sequence update: | December 15, 2003 | |
Last modified: | April 7, 2021 | |
This is version 66 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families