Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 89 (11 Dec 2019)
Sequence version 3 (22 Jan 2014)
Previous versions | rss
Help videoAdd a publicationFeedback
Protein

Ergothioneine biosynthesis protein 1

Gene

egt-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the SAM-dependent triple methylation of the alpha-amino group of histidine to form hercynine and subsequent conjugation with cysteine and oxygen to form hercynylcysteine sulfoxide, the first two steps in the biosynthesis pathway of ergothioneine (PubMed:22209968). Ergothioneine is an antioxidant against peroxide in conidia and contributes to conidial longevity (PubMed:22209968, PubMed:25446508).2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Fe2+1 PublicationNote: Binds 1 Fe2+ ion per monomer.1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=436 µM for hercynine1 Publication
  2. KM=603 µM for cysteine1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: ergothioneine biosynthesis

    This protein is involved in the pathway ergothioneine biosynthesis, which is part of Amino-acid biosynthesis.1 Publication
    View all proteins of this organism that are known to be involved in the pathway ergothioneine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei88L-histidineBy similarity1
    Binding sitei119S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
    Binding sitei125S-adenosyl-L-methionineBy similarity1
    Binding sitei146S-adenosyl-L-methionineBy similarity1
    Binding sitei202L-histidineBy similarity1
    Binding sitei242L-histidineBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi413Iron; via tele nitrogenBy similarity1
    Metal bindingi506Iron; via tele nitrogenBy similarity1
    Metal bindingi510Iron; via tele nitrogenBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionMethyltransferase, Oxidoreductase, Transferase
    LigandIron, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MetaCyc:MONOMER-18845

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA01014

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Ergothioneine biosynthesis protein 11 Publication
    Including the following 2 domains:
    L-histidine N(alpha)-methyltransferase1 Publication (EC:2.1.1.441 Publication)
    Hercynylcysteine S-oxide synthase1 Publication (EC:1.14.99.511 Publication1 Publication)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:egt-11 Publication
    ORF Names:NCU04343
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri367110 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000001805 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 4, Linkage Group IV

    Organism-specific databases

    Eukaryotic Pathogen Database Resources

    More...
    EuPathDBi
    FungiDB:NCU04343

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Does not produce ergothioneine in either conidia nor mycelia.1 Publication

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004349871 – 876Ergothioneine biosynthesis protein 1Add BLAST876

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q7RX33

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni36 – 350L-histidine N(alpha)-methyltransferaseCuratedAdd BLAST315
    Regioni315 – 317L-histidine bindingBy similarity3
    Regioni378 – 874Hercynylcysteine S-oxide synthaseCuratedAdd BLAST497

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    In the N-terminal section; belongs to the methyltransferase superfamily. EgtD family.Curated
    In the C-terminal section; belongs to the EgtB family.Curated

    Phylogenomic databases

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000167160

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    Q7RX33

    KEGG Orthology (KO)

    More...
    KOi
    K20246

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.90.1580.10, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR016187 CTDL_fold
    IPR019257 MeTrfase_dom
    IPR017805 SAM_MeTrfase_EasF-type_put
    IPR005532 SUMF_dom
    IPR042095 SUMF_sf

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF03781 FGE-sulfatase, 2 hits
    PF10017 Methyltransf_33, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF56436 SSF56436, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR03439 methyl_EasF, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    Q7RX33-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MPSAESMTPS SALGQLKATG QHVLSKLQQQ TSNADIIDIR RVAVEINLKT
    60 70 80 90 100
    EITSMFRPKD GPRQLPTLLL YNERGLQLFE RITYLEEYYL TNDEIKILTK
    110 120 130 140 150
    HATEMASFIP SGAMIIELGS GNLRKVNLLL EALDNAGKAI DYYALDLSRE
    160 170 180 190 200
    ELERTLAQVP SYKHVKCHGL LGTYDDGRDW LKAPENINKQ KCILHLGSSI
    210 220 230 240 250
    GNFNRSDAAT FLKGFTDVLG PNDKMLIGVD ACNDPARVYH AYNDKVGITH
    260 270 280 290 300
    EFILNGLRNA NEIIGETAFI EGDWRVIGEY VYDEEGGRHQ AFYAPTRDTM
    310 320 330 340 350
    VMGELIRSHD RIQIEQSLKY SKEESERLWS TAGLEQVSEW TYGNEYGLHL
    360 370 380 390 400
    LAKSRMSFSL IPSVYARSAL PTLDDWEALW ATWDVVTRQM LPQEELLEKP
    410 420 430 440 450
    IKLRNACIFY LGHIPTFLDI QLTKTTKQAP SEPAHFCKIF ERGIDPDVDN
    460 470 480 490 500
    PELCHAHSEI PDEWPPVEEI LTYQETVRSR LRGLYAHGIA NIPRNVGRAI
    510 520 530 540 550
    WVGFEHELMH IETLLYMMLQ SDKTLIPTHI PRPDFDKLAR KAESERVPNQ
    560 570 580 590 600
    WFKIPAQEIT IGLDDPEDGS DINKHYGWDN EKPPRRVQVA AFQAQGRPIT
    610 620 630 640 650
    NEEYAQYLLE KNIDKLPASW ARLDNENISN GTTNSVSGHH SNRTSKQQLP
    660 670 680 690 700
    SSFLEKTAVR TVYGLVPLKH ALDWPVFASY DELAGCAAYM GGRIPTFEET
    710 720 730 740 750
    RSIYAYADAL KKKKEAERQL GRTVPAVNAH LTNNGVEITP PSSPSSETPA
    760 770 780 790 800
    ESSSPSDSNT TLITTEDLFS DLDGANVGFH NWHPMPITSK GNTLVGQGEL
    810 820 830 840 850
    GGVWEWTSSV LRKWEGFEPM ELYPGYTADF FDEKHNIVLG GSWATHPRIA
    860 870
    GRKSFVNWYQ RNYPYAWVGA RVVRDL
    Length:876
    Mass (Da):99,025
    Last modified:January 22, 2014 - v3
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i694FEB44522B96BD
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    CM002239 Genomic DNA Translation: EAA27088.3

    NCBI Reference Sequences

    More...
    RefSeqi
    XP_956324.3, XM_951231.3

    Genome annotation databases

    Ensembl fungal genome annotation project

    More...
    EnsemblFungii
    EAA27088; EAA27088; NCU04343

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    3872471

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ncr:NCU04343

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CM002239 Genomic DNA Translation: EAA27088.3
    RefSeqiXP_956324.3, XM_951231.3

    3D structure databases

    SMRiQ7RX33
    ModBaseiSearch...

    Genome annotation databases

    EnsemblFungiiEAA27088; EAA27088; NCU04343
    GeneIDi3872471
    KEGGincr:NCU04343

    Organism-specific databases

    EuPathDBiFungiDB:NCU04343

    Phylogenomic databases

    HOGENOMiHOG000167160
    InParanoidiQ7RX33
    KOiK20246

    Enzyme and pathway databases

    UniPathwayiUPA01014
    BioCyciMetaCyc:MONOMER-18845

    Family and domain databases

    Gene3Di3.90.1580.10, 1 hit
    InterProiView protein in InterPro
    IPR016187 CTDL_fold
    IPR019257 MeTrfase_dom
    IPR017805 SAM_MeTrfase_EasF-type_put
    IPR005532 SUMF_dom
    IPR042095 SUMF_sf
    PfamiView protein in Pfam
    PF03781 FGE-sulfatase, 2 hits
    PF10017 Methyltransf_33, 1 hit
    SUPFAMiSSF56436 SSF56436, 1 hit
    TIGRFAMsiTIGR03439 methyl_EasF, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiEGT1_NEUCR
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q7RX33
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 9, 2015
    Last sequence update: January 22, 2014
    Last modified: December 11, 2019
    This is version 89 of the entry and version 3 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again