UniProtKB - Q7RTS1 (BHA15_HUMAN)
Protein
Class A basic helix-loop-helix protein 15
Gene
BHLHA15
Organism
Homo sapiens (Human)
Status
Functioni
Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3' (By similarity).By similarity
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: Ensembl
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- identical protein binding Source: Ensembl
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
GO - Biological processi
- calcium-mediated signaling Source: Ensembl
- cell-cell signaling Source: Ensembl
- cell maturation Source: Ensembl
- cellular response to glucose starvation Source: UniProtKB
- endoplasmic reticulum unfolded protein response Source: UniProtKB
- glucose homeostasis Source: Ensembl
- Golgi organization Source: Ensembl
- G protein-coupled receptor signaling pathway Source: Ensembl
- intracellular distribution of mitochondria Source: Ensembl
- mitochondrial calcium ion transmembrane transport Source: Ensembl
- negative regulation of myotube differentiation Source: UniProtKB
- neuron differentiation Source: GO_Central
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q7RTS1 |
Names & Taxonomyi
Protein namesi | Recommended name: Class A basic helix-loop-helix protein 15Short name: bHLHa15 Alternative name(s): Class B basic helix-loop-helix protein 8 Short name: bHLHb8 Muscle, intestine and stomach expression 1 Short name: MIST-1 |
Gene namesi | Name:BHLHA15 Synonyms:BHLHB8, MIST1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:22265, BHLHA15 |
MIMi | 608606, gene |
neXtProti | NX_Q7RTS1 |
VEuPathDBi | HostDB:ENSG00000180535.3 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 168620 |
OpenTargetsi | ENSG00000180535 |
PharmGKBi | PA164716601 |
Miscellaneous databases
Pharosi | Q7RTS1, Tbio |
Genetic variation databases
BioMutai | BHLHA15 |
DMDMi | 50400944 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127150 | 1 – 189 | Class A basic helix-loop-helix protein 15Add BLAST | 189 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 25 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q7RTS1 |
MassIVEi | Q7RTS1 |
MaxQBi | Q7RTS1 |
PaxDbi | Q7RTS1 |
PeptideAtlasi | Q7RTS1 |
PRIDEi | Q7RTS1 |
ProteomicsDBi | 68891 |
PTM databases
iPTMneti | Q7RTS1 |
PhosphoSitePlusi | Q7RTS1 |
Expressioni
Tissue specificityi
Expressed in brain, liver, spleen and skeletal muscle.1 Publication
Gene expression databases
Bgeei | ENSG00000180535, Expressed in body of pancreas and 100 other tissues |
Organism-specific databases
HPAi | ENSG00000180535, Group enriched (pancreas, salivary gland) |
Interactioni
Subunit structurei
Forms homodimers or heterodimers with TCF3 gene products E12 and E47. These dimers bind to the E-box site, however, heterodimer with MYOD1 does not bind target DNA (By similarity).
By similarityBinary interactionsi
Q7RTS1
With | #Exp. | IntAct |
---|---|---|
MYOG [P15173] | 3 | EBI-8844218,EBI-3906629 |
GO - Molecular functioni
- identical protein binding Source: Ensembl
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 127970, 46 interactors |
IntActi | Q7RTS1, 43 interactors |
MINTi | Q7RTS1 |
STRINGi | 9606.ENSP00000476312 |
Miscellaneous databases
RNActi | Q7RTS1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 75 – 127 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Domaini
Lacks a classic transcription activation domain and instead possesses an N-terminal region capable of inhibiting heterologous activators.By similarity
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000161824 |
HOGENOMi | CLU_097977_2_0_1 |
InParanoidi | Q7RTS1 |
OMAi | HRYSTQI |
OrthoDBi | 1528350at2759 |
PhylomeDBi | Q7RTS1 |
TreeFami | TF315153 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
Q7RTS1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTKNRPPRR RAPVQDTEAT PGEGTPDGSL PNPGPEPAKG LRSRPARAAA
60 70 80 90 100
RAPGEGRRRR PGPSGPGGRR DSSIQRRLES NERERQRMHK LNNAFQALRE
110 120 130 140 150
VIPHVRADKK LSKIETLTLA KNYIKSLTAT ILTMSSSRLP GLEGPGPKLY
160 170 180
QHYQQQQQVA GGALGATEAQ PQGHLQRYST QIHSFREGT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC025605 Genomic DNA No translation available. CH236956 Genomic DNA Translation: EAL23893.1 BC113394 mRNA Translation: AAI13395.1 BC113396 mRNA Translation: AAI13397.1 BK000276 mRNA Translation: DAA01056.1 |
CCDSi | CCDS5655.1 |
RefSeqi | NP_803238.1, NM_177455.3 |
Genome annotation databases
Ensembli | ENST00000314018; ENSP00000326391; ENSG00000180535 ENST00000609256; ENSP00000476312; ENSG00000180535 |
GeneIDi | 168620 |
KEGGi | hsa:168620 |
UCSCi | uc003upe.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC025605 Genomic DNA No translation available. CH236956 Genomic DNA Translation: EAL23893.1 BC113394 mRNA Translation: AAI13395.1 BC113396 mRNA Translation: AAI13397.1 BK000276 mRNA Translation: DAA01056.1 |
CCDSi | CCDS5655.1 |
RefSeqi | NP_803238.1, NM_177455.3 |
3D structure databases
SMRi | Q7RTS1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 127970, 46 interactors |
IntActi | Q7RTS1, 43 interactors |
MINTi | Q7RTS1 |
STRINGi | 9606.ENSP00000476312 |
PTM databases
iPTMneti | Q7RTS1 |
PhosphoSitePlusi | Q7RTS1 |
Genetic variation databases
BioMutai | BHLHA15 |
DMDMi | 50400944 |
Proteomic databases
jPOSTi | Q7RTS1 |
MassIVEi | Q7RTS1 |
MaxQBi | Q7RTS1 |
PaxDbi | Q7RTS1 |
PeptideAtlasi | Q7RTS1 |
PRIDEi | Q7RTS1 |
ProteomicsDBi | 68891 |
Protocols and materials databases
Antibodypediai | 30223, 116 antibodies |
Genome annotation databases
Ensembli | ENST00000314018; ENSP00000326391; ENSG00000180535 ENST00000609256; ENSP00000476312; ENSG00000180535 |
GeneIDi | 168620 |
KEGGi | hsa:168620 |
UCSCi | uc003upe.2, human |
Organism-specific databases
CTDi | 168620 |
DisGeNETi | 168620 |
GeneCardsi | BHLHA15 |
HGNCi | HGNC:22265, BHLHA15 |
HPAi | ENSG00000180535, Group enriched (pancreas, salivary gland) |
MIMi | 608606, gene |
neXtProti | NX_Q7RTS1 |
OpenTargetsi | ENSG00000180535 |
PharmGKBi | PA164716601 |
VEuPathDBi | HostDB:ENSG00000180535.3 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000161824 |
HOGENOMi | CLU_097977_2_0_1 |
InParanoidi | Q7RTS1 |
OMAi | HRYSTQI |
OrthoDBi | 1528350at2759 |
PhylomeDBi | Q7RTS1 |
TreeFami | TF315153 |
Enzyme and pathway databases
PathwayCommonsi | Q7RTS1 |
Miscellaneous databases
BioGRID-ORCSi | 168620, 43 hits in 1005 CRISPR screens |
GeneWikii | BHLHB8 |
GenomeRNAii | 168620 |
Pharosi | Q7RTS1, Tbio |
PROi | PR:Q7RTS1 |
RNActi | Q7RTS1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000180535, Expressed in body of pancreas and 100 other tissues |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BHA15_HUMAN | |
Accessioni | Q7RTS1Primary (citable) accession number: Q7RTS1 Secondary accession number(s): A4D271, Q14DE4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | December 15, 2003 | |
Last modified: | April 7, 2021 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot