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Protein

Phosphoribosylformylglycinamidine synthase

Gene

purL

Organism
Photorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.UniRule annotation

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei677ATP; via carbonyl oxygenUniRule annotation1
Metal bindingi678MagnesiumUniRule annotation1
Metal bindingi717MagnesiumUniRule annotation1
Metal bindingi721MagnesiumUniRule annotation1
Metal bindingi884MagnesiumUniRule annotation1
Binding sitei886ATPUniRule annotation1
Active sitei1135NucleophileUniRule annotation1
Active sitei1260UniRule annotation1
Active sitei1262UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi306 – 317ATPUniRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPLUM243265:PLU_RS16495-MONOMER
UniPathwayi
UPA00074;UER00128

Protein family/group databases

MEROPSiC56.972

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthaseUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Short name:
FGAMSUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferaseUniRule annotation
Short name:
FGAR amidotransferaseUniRule annotation
Short name:
FGAR-ATUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:plu3317
OrganismiPhotorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)
Taxonomic identifieri243265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesMorganellaceaePhotorhabdus
Proteomesi
  • UP000002514 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002645841 – 1295Phosphoribosylformylglycinamidine synthaseAdd BLAST1295

Proteomic databases

PRIDEiQ7N1Z4

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243265.plu3317

Structurei

3D structure databases

ProteinModelPortaliQ7N1Z4
SMRiQ7N1Z4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1042 – 1295Glutamine amidotransferase type-1UniRule annotationAdd BLAST254

Sequence similaritiesi

In the N-terminal section; belongs to the FGAMS family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4107QIK Bacteria
COG0046 LUCA
COG0047 LUCA
HOGENOMiHOG000261359
KOiK01952
OrthoDBiPOG091H021O

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.40.50.880, 1 hit
3.90.650.10, 2 hits
HAMAPiMF_00419 PurL_1, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR010073 PRibForGlyAmidine_synth
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR036921 PurM-like_N_sf
IPR036604 PurS-like_sf
PANTHERiPTHR10099:SF5 PTHR10099:SF5, 1 hit
PfamiView protein in Pfam
PF02769 AIRS_C, 2 hits
SUPFAMiSSF52317 SSF52317, 1 hit
SSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
SSF82697 SSF82697, 1 hit
TIGRFAMsiTIGR01735 FGAM_synt, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q7N1Z4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKILRGSPAL SAFRITKLLS VCQEQQLPVN DIYAEYVHFA EINASLSDVD
60 70 80 90 100
SAKLQQLLKY GPSLAEHEPQ GTLLLVTPRP GTISPWSSKA TDIAHNCGLS
110 120 130 140 150
QVVRLERGVA YYIQSGEMSD TQWQILSSLL HDRMMETVFT QLEQAEKLFS
160 170 180 190 200
RQQPVPLKRI DILQAGRSAL ETANIELGLA LASDEIDYLM DAFQKLGRNP
210 220 230 240 250
TDVELYMFAQ ANSEHCRHKI FNADWIIDSQ AQPKSLFKMI KNTYEQTPDY
260 270 280 290 300
VLSAYKDNAA VMEGSAVGRF FASAENGSYD YHQEQAHILM KVETHNHPTA
310 320 330 340 350
ISPWPGASTG SGGEIRDEGA TGRGAKPKAG LVGFSVSNLR IPGFEQPWEE
360 370 380 390 400
DFGKPERIVS ALDIMMEGPL GGAAFNNEFG RPALLGYFRT YEEKVNSHNG
410 420 430 440 450
SELRGYHKPI MLAGGIGNIR DEHVKKGEIS VGAKLIVLGG PSMNIGLGGG
460 470 480 490 500
AASSMASGQS DADLDFASVQ RDNPEMERRC QEVIDRCWQL GENNPILFIH
510 520 530 540 550
DVGAGGLSNA MPELVSDGGR GGRFELRKIL NDEPGMSPLE VWCNESQERY
560 570 580 590 600
VLAVAPEQLP LFEEICRRER APYAIIGEAT EERHLLLNDE HFDNQPIDMP
610 620 630 640 650
LDVLLGKTPK MLRDVTTLKA SGESLERRDI DLAEAVKRIM HLPAVAEKTF
660 670 680 690 700
LITIGDRSVT GMVSRDQMVG PWQIPVADCA VTTASLDSYY GEAMSMGERA
710 720 730 740 750
PVALLDFAAS ARMAVGEALT NIASAYIQDL KRIKLSANWM SAAGHPGEDA
760 770 780 790 800
GLYAAVKAVG EELCPALGLT IPVGKDSMSM KTRWHDQGEE REMTAPLSLV
810 820 830 840 850
ITAFARVEDV RRTVTPELST DEDNALLLID LGQGKNTLGG TALAQVYRLL
860 870 880 890 900
GNKTADVRSA EQLAGFFNAI QQLIAEQKLL AYHDRSDGGL LVTLAEMAFA
910 920 930 940 950
GHCGIEADIS VFDEDILAGL FTEELGAVVQ IRASDRGFVE SILAEHGLAD
960 970 980 990 1000
CVHYLGKAQA GDDFVIFSGN TEVYRQNRST LRLWWAETTW QMQRLRDNPA
1010 1020 1030 1040 1050
CADQEHQAKQ DNQDPGLNVK LTFDISEDIA APYILQQVRP KVAVLREQGV
1060 1070 1080 1090 1100
NSHVEMAAAF HRAGFEAIDV HMSDLLSGRI GLSQFQTLVA CGGFSYGDVL
1110 1120 1130 1140 1150
GAGEGWAKSI LFNERVRDQF AVFFARPDTL ALGVCNGCQM MSNLRELIPG
1160 1170 1180 1190 1200
AEHWPRFVRN RSERFEARFS LVEITDSPSL FLQDMVGSRI PIAVSHGEGQ
1210 1220 1230 1240 1250
VEFRNRQHLE MLESNQLVAL RYVNNYGQVT ENYPANPNGS VNGITAVTSL
1260 1270 1280 1290
DGRATVMMPH PERVSRTVNN SWHPDEWRED GPWMRIFRNA RKQLG
Length:1,295
Mass (Da):142,784
Last modified:December 12, 2006 - v2
Checksum:iD768C7E94B790CC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571870 Genomic DNA Translation: CAE15691.1
RefSeqiWP_041381011.1, NC_005126.1

Genome annotation databases

EnsemblBacteriaiCAE15691; CAE15691; plu3317
KEGGiplu:plu3317

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571870 Genomic DNA Translation: CAE15691.1
RefSeqiWP_041381011.1, NC_005126.1

3D structure databases

ProteinModelPortaliQ7N1Z4
SMRiQ7N1Z4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243265.plu3317

Protein family/group databases

MEROPSiC56.972

Proteomic databases

PRIDEiQ7N1Z4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE15691; CAE15691; plu3317
KEGGiplu:plu3317

Phylogenomic databases

eggNOGiENOG4107QIK Bacteria
COG0046 LUCA
COG0047 LUCA
HOGENOMiHOG000261359
KOiK01952
OrthoDBiPOG091H021O

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00128

BioCyciPLUM243265:PLU_RS16495-MONOMER

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.40.50.880, 1 hit
3.90.650.10, 2 hits
HAMAPiMF_00419 PurL_1, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR010073 PRibForGlyAmidine_synth
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR036921 PurM-like_N_sf
IPR036604 PurS-like_sf
PANTHERiPTHR10099:SF5 PTHR10099:SF5, 1 hit
PfamiView protein in Pfam
PF02769 AIRS_C, 2 hits
SUPFAMiSSF52317 SSF52317, 1 hit
SSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
SSF82697 SSF82697, 1 hit
TIGRFAMsiTIGR01735 FGAM_synt, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPUR4_PHOLL
AccessioniPrimary (citable) accession number: Q7N1Z4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: October 10, 2018
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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