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UniProtKB - Q7MX71 (MEGL_PORGI)
Protein
L-methionine gamma-lyase
Gene
mgl
Organism
Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
Status
Functioni
Catalyzes the alpha,gamma-elimination of L-methionine to produce methanethiol, 2-oxobutanoate and ammonia; methanethiol (methyl mercaptan) is considered to be one of the main causes of the oral malodor in periodontal disease and may also play a role in the pathogenicity of P.gingivalis in that disease (PubMed:11083813).
Is also able to catalyze the alpha,gamma-elimination of L-homocysteine (By similarity).
By similarity1 PublicationCatalytic activityi
Cofactori
pyridoxal 5'-phosphateBy similarityCurated
Kineticsi
- KM=23.1 mM for L-methionine1 Publication
- Vmax=21.9 µmol/min/mg enzyme1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 114 | SubstrateBy similarity | 1 | |
Binding sitei | 376 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- homocysteine desulfhydrase activity Source: UniProtKB-EC
- methionine gamma-lyase activity Source: UniProtKB-EC
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- transsulfuration Source: InterPro
Keywordsi
Molecular function | Lyase |
Biological process | Virulence |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BRENDAi | 4.4.1.11, 756 |
SABIO-RKi | Q7MX71 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mgl1 Publication Synonyms:megLImported Ordered Locus Names:PG_0343Imported |
Organismi | Porphyromonas gingivalis (strain ATCC BAA-308 / W83) |
Taxonomic identifieri | 242619 [NCBI] |
Taxonomic lineagei | Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Porphyromonas › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene show a marked decrease in the formation of methyl mercaptan from L-methionine and decreased virulence compared with the wild-type strain W83. The hydrogen sulfide content in the culture supernatants of mutant and wild-type strains is similar.1 Publication
Miscellaneous databases
PHI-basei | PHI:7938 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000436014 | 1 – 399 | L-methionine gamma-lyaseAdd BLAST | 399 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 212 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Interactioni
Subunit structurei
Homotetramer; dimer of active dimers.
By similarityProtein-protein interaction databases
STRINGi | 242619.PG_0343 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 59 – 61 | Pyridoxal phosphate binding; shared with dimeric partnerBy similarity | 3 | |
Regioni | 89 – 90 | Pyridoxal phosphate bindingBy similarity | 2 | |
Regioni | 209 – 211 | Pyridoxal phosphate bindingBy similarity | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0626, Bacteria |
HOGENOMi | CLU_018986_2_3_10 |
OrthoDBi | 637281at2 |
Family and domain databases
CDDi | cd00614, CGS_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000277, Cys/Met-Metab_PyrdxlP-dep_enz IPR006237, L-Met_gamma_lys IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR11808, PTHR11808, 1 hit |
Pfami | View protein in Pfam PF01053, Cys_Met_Meta_PP, 1 hit |
PIRSFi | PIRSF001434, CGS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR01328, met_gam_lyase, 1 hit |
PROSITEi | View protein in PROSITE PS00868, CYS_MET_METAB_PP, 1 hit |
i Sequence
Sequence statusi: Complete.
Q7MX71-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKEDLMRSG FATRAIHGGA IENAFGCLAT PIYQTSTFVF DTAEQGGRRF
60 70 80 90 100
AGEEDGYIYT RLGNPNCTQV EEKLAMLEGG EAAASASSGI GAISSAIWVC
110 120 130 140 150
VKAGDHIVAG KTLYGCTFAF LTHGLSRYGV EVTLVDTRHP EEVEAAIRPN
160 170 180 190 200
TKLVYLETPA NPNMYLTDIK AVCDIAHKHE GVRVMVDNTY CTPYICRPLE
210 220 230 240 250
LGADIVVHSA TKYLNGHGDV IAGFVVGKED YIKEVKLVGV KDLTGANMSP
260 270 280 290 300
FDAYLISRGM KTLQIRMEQH CRNAQTVAEF LEKHPAVEAV YFPGLPSFPQ
310 320 330 340 350
YELAKKQMAL PGAMIAFEVK GGCEAGKKLM NNLHLCSLAV SLGDTETLIQ
360 370 380 390
HPASMTHSPY TPEERAASDI SEGLVRLSVG LENVEDIIAD LKHGLDSLI
Sequence cautioni
The sequence AAQ65554 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE015924 Genomic DNA Translation: AAQ65554.1 Different initiation. |
RefSeqi | WP_005873786.1, NC_002950.2 |
Genome annotation databases
EnsemblBacteriai | AAQ65554; AAQ65554; PG_0343 |
GeneIDi | 29256789 |
KEGGi | pgi:PG_0343 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE015924 Genomic DNA Translation: AAQ65554.1 Different initiation. |
RefSeqi | WP_005873786.1, NC_002950.2 |
3D structure databases
AlphaFoldDBi | Q7MX71 |
SMRi | Q7MX71 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 242619.PG_0343 |
Genome annotation databases
EnsemblBacteriai | AAQ65554; AAQ65554; PG_0343 |
GeneIDi | 29256789 |
KEGGi | pgi:PG_0343 |
Phylogenomic databases
eggNOGi | COG0626, Bacteria |
HOGENOMi | CLU_018986_2_3_10 |
OrthoDBi | 637281at2 |
Enzyme and pathway databases
BRENDAi | 4.4.1.11, 756 |
SABIO-RKi | Q7MX71 |
Miscellaneous databases
PHI-basei | PHI:7938 |
Family and domain databases
CDDi | cd00614, CGS_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000277, Cys/Met-Metab_PyrdxlP-dep_enz IPR006237, L-Met_gamma_lys IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR11808, PTHR11808, 1 hit |
Pfami | View protein in Pfam PF01053, Cys_Met_Meta_PP, 1 hit |
PIRSFi | PIRSF001434, CGS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR01328, met_gam_lyase, 1 hit |
PROSITEi | View protein in PROSITE PS00868, CYS_MET_METAB_PP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MEGL_PORGI | |
Accessioni | Q7MX71Primary (citable) accession number: Q7MX71 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2016 |
Last sequence update: | April 13, 2016 | |
Last modified: | May 25, 2022 | |
This is version 103 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families