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Protein

Phosphomethylpyrimidine synthase

Gene

thiC

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.UniRule annotation

Catalytic activityi

5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphomethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei80SubstrateUniRule annotation1
Binding sitei109SubstrateUniRule annotation1
Binding sitei138SubstrateUniRule annotation1
Binding sitei174SubstrateUniRule annotation1
Binding sitei274SubstrateUniRule annotation1
Metal bindingi278ZincUniRule annotation1
Binding sitei301SubstrateUniRule annotation1
Metal bindingi342ZincUniRule annotation1
Metal bindingi422Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi425Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi430Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

BioCyciWSUC273121:G1GTD-1834-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomethylpyrimidine synthaseUniRule annotation (EC:4.1.99.17UniRule annotation)
Alternative name(s):
Hydroxymethylpyrimidine phosphate synthaseUniRule annotation
Short name:
HMP-P synthaseUniRule annotation
Short name:
HMP-phosphate synthaseUniRule annotation
Short name:
HMPP synthaseUniRule annotation
Thiamine biosynthesis protein ThiCUniRule annotation
Gene namesi
Name:thiCUniRule annotation
Ordered Locus Names:WS1941
OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Taxonomic identifieri273121 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
Proteomesi
  • UP000000422 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001528501 – 449Phosphomethylpyrimidine synthaseAdd BLAST449

Proteomic databases

PRIDEiQ7M818

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273121.WS1941

Structurei

3D structure databases

ProteinModelPortaliQ7M818
SMRiQ7M818
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 196Substrate bindingUniRule annotation3
Regioni235 – 238Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the ThiC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CBF Bacteria
COG0422 LUCA
HOGENOMiHOG000224483
KOiK03147
OMAiFMAIHTG
OrthoDBiPOG091H02FB

Family and domain databases

Gene3Di3.20.20.540, 1 hit
HAMAPiMF_00089 ThiC, 1 hit
InterProiView protein in InterPro
IPR037509 ThiC
IPR038521 ThiC/Bza_sf
IPR002817 ThiC/BzaA/B
PANTHERiPTHR30557 PTHR30557, 1 hit
PfamiView protein in Pfam
PF01964 ThiC_Rad_SAM, 1 hit
SFLDiSFLDF00407 phosphomethylpyrimidine_syntha, 1 hit
TIGRFAMsiTIGR00190 thiC, 1 hit

Sequencei

Sequence statusi: Complete.

Q7M818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQSWIEKRK NDSVRTQLHY ARAGIITEEM QAVANVEGLS EELIRSEVAR
60 70 80 90 100
GRLVIPANIH HLSLSPMGIG VAARTKINAN IGSSSLASCI EEEIEKTLVS
110 120 130 140 150
IKYGADTIMD LSTGGDLDAI RCAVIEHSSV PIGTVPIYQI LHDVQGDIKN
160 170 180 190 200
LTIDSMLEVM ERQARQGVSY FTIHCGFLLE HLPKVAKRKM GIVSRGGSLM
210 220 230 240 250
ASYMMHYHKQ NPFYEAFDEI LAICREYDVT LSLGDSLRPG CLADASDEAQ
260 270 280 290 300
LAELKVLGEL TLRAWEKDVQ VMIEGPGHVP MNQIERNVKL QKELCHEAPF
310 320 330 340 350
YVLGPLVTDI AAGYDHIASA IGAAIAAWKG VAMLCYVTPK EHLGLPNAKD
360 370 380 390 400
VREGILAYKI AAHAADIARG RIGARDRDDA MSDARYAFDW NRQFELALDP
410 420 430 440
DRAKEYHDES LPQEVFKEAK FCSMCGPKFC SYKISQEIVS KHASGESLT
Length:449
Mass (Da):49,657
Last modified:December 15, 2003 - v1
Checksum:iDB137D4EFE4DB6A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571662 Genomic DNA Translation: CAE10947.1
RefSeqiWP_011139730.1, NC_005090.1

Genome annotation databases

EnsemblBacteriaiCAE10947; CAE10947; WS1941
KEGGiwsu:WS1941

Similar proteinsi

Entry informationi

Entry nameiTHIC_WOLSU
AccessioniPrimary (citable) accession number: Q7M818
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: December 15, 2003
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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