UniProtKB - Q7M730 (SCN4B_RAT)
Protein
Sodium channel subunit beta-4
Gene
Scn4b
Organism
Rattus norvegicus (Rat)
Status
Functioni
Modulates channel gating kinetics. Causes negative shifts in the voltage dependence of activation of certain alpha sodium channels, but does not affect the voltage dependence of inactivation. Modulates the susceptibility of the sodium channel to inhibition by toxic peptides from spider, scorpion, wasp and sea anemone venom.1 Publication
GO - Molecular functioni
- ion channel binding Source: RGD
- sodium channel regulator activity Source: UniProtKB
- voltage-gated ion channel activity Source: UniProtKB-KW
- voltage-gated sodium channel activity Source: RGD
- voltage-gated sodium channel activity involved in cardiac muscle cell action potential Source: RGD
GO - Biological processi
- AV node cell action potential Source: RGD
- cardiac muscle cell action potential involved in contraction Source: RGD
- cardiac muscle contraction Source: RGD
- membrane depolarization during cardiac muscle cell action potential Source: RGD
- positive regulation of sodium ion transport Source: RGD
- regulation of heart rate by cardiac conduction Source: RGD
- regulation of sodium ion transmembrane transporter activity Source: UniProtKB
- regulation of ventricular cardiac muscle cell membrane repolarization Source: RGD
- sodium ion transmembrane transport Source: RGD
- sodium ion transport Source: RGD
Keywordsi
Molecular function | Ion channel, Sodium channel, Voltage-gated channel |
Biological process | Ion transport, Sodium transport, Transport |
Ligand | Sodium |
Enzyme and pathway databases
Reactomei | R-RNO-5576892, Phase 0 - rapid depolarisation |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium channel subunit beta-4 |
Gene namesi | Name:Scn4b |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 631404, Scn4b |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication
Plasma Membrane
- intrinsic component of plasma membrane Source: UniProtKB
- plasma membrane Source: RGD
- voltage-gated sodium channel complex Source: UniProtKB
Other locations
- intercalated disc Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 31 – 161 | ExtracellularSequence analysisAdd BLAST | 131 | |
Transmembranei | 162 – 182 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 183 – 228 | CytoplasmicSequence analysisAdd BLAST | 46 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 30 | Sequence analysisAdd BLAST | 30 | |
ChainiPRO_0000014939 | 31 – 228 | Sodium channel subunit beta-4Add BLAST | 198 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 45 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 53 ↔ 131 | PROSITE-ProRule annotationBy similarity | ||
Disulfide bondi | 58 | Interchain; with alpha subunitPROSITE-ProRule annotationBy similarity1 Publication | ||
Glycosylationi | 71 | N-linked (GlcNAc...) asparagineCombined sources | 1 | |
Glycosylationi | 113 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 142 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Contains an interchain disulfide bond with SCN2A.
N-glycosylated.
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q7M730 |
PRIDEi | Q7M730 |
PTM databases
GlyGeni | Q7M730, 4 sites, 2 N-linked glycans (1 site) |
iPTMneti | Q7M730 |
PhosphoSitePlusi | Q7M730 |
UniCarbKBi | Q7M730 |
Expressioni
Tissue specificityi
Expressed at a high level in dorsal root ganglia, at a lower level in brain, spinal cord, skeletal muscle and heart.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000026679, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | Q7M730, RN |
Interactioni
Subunit structurei
The voltage-sensitive sodium channel consists of an ion conducting pore forming alpha-subunit (SCN2A) regulated by one or more beta subunits (SCN1B, SCN2B, SCN3B and SCN4B). SCN1B and SCN3B are non-covalently associated with SCN2A. SCN2B and SCN4B are disulfide-linked to SCN2A (PubMed:12930796, PubMed:26894959).
2 PublicationsGO - Molecular functioni
- ion channel binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000033284 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 31 – 148 | Ig-like C2-typeAdd BLAST | 118 |
Sequence similaritiesi
Belongs to the sodium channel auxiliary subunit SCN4B (TC 8.A.17) family. [View classification]Curated
Keywords - Domaini
Immunoglobulin domain, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QTZ6, Eukaryota |
GeneTreei | ENSGT01000000214451 |
HOGENOMi | CLU_104235_0_0_1 |
InParanoidi | Q7M730 |
OMAi | TWYFNST |
OrthoDBi | 1219179at2759 |
PhylomeDBi | Q7M730 |
TreeFami | TF331728 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set IPR000920, Myelin_P0-rel |
PANTHERi | PTHR13869, PTHR13869, 1 hit |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q7M730-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRAGNRGNT QARWLGIGLL GLFLLPMYLS LEVSVGKATT IYAINGSAIL
60 70 80 90 100
LPCTFSSCYG FENLYFRWSY NNSETSRILI DGIVKNDKSD PKVRVKDDDR
110 120 130 140 150
ITLEGSTKEK MNNISILLSD LEFSDTGRYT CFVRNPKEKD LNNSATIFLQ
160 170 180 190 200
VVDKLEEVDN TVTLIILAVV GGVIGLLVCI LLLKKLITFI LKKTREKKKE
210 220
CLVSSSGNDN TENGLPGSKA EEKPPTKV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC129680 Genomic DNA No translation available. AC129457 Genomic DNA No translation available. AC136555 Genomic DNA No translation available. AF544988 mRNA Translation: AAO62631.1 BK001030 mRNA Translation: DAA01204.1 |
RefSeqi | NP_001008880.1, NM_001008880.1 |
Genome annotation databases
Ensembli | ENSRNOT00000030152; ENSRNOP00000033284; ENSRNOG00000026679 |
GeneIDi | 315611 |
KEGGi | rno:315611 |
UCSCi | RGD:631404, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC129680 Genomic DNA No translation available. AC129457 Genomic DNA No translation available. AC136555 Genomic DNA No translation available. AF544988 mRNA Translation: AAO62631.1 BK001030 mRNA Translation: DAA01204.1 |
RefSeqi | NP_001008880.1, NM_001008880.1 |
3D structure databases
SMRi | Q7M730 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000033284 |
PTM databases
GlyGeni | Q7M730, 4 sites, 2 N-linked glycans (1 site) |
iPTMneti | Q7M730 |
PhosphoSitePlusi | Q7M730 |
UniCarbKBi | Q7M730 |
Proteomic databases
PaxDbi | Q7M730 |
PRIDEi | Q7M730 |
Protocols and materials databases
ABCDi | Q7M730, 1 sequenced antibody |
Genome annotation databases
Ensembli | ENSRNOT00000030152; ENSRNOP00000033284; ENSRNOG00000026679 |
GeneIDi | 315611 |
KEGGi | rno:315611 |
UCSCi | RGD:631404, rat |
Organism-specific databases
CTDi | 6330 |
RGDi | 631404, Scn4b |
Phylogenomic databases
eggNOGi | ENOG502QTZ6, Eukaryota |
GeneTreei | ENSGT01000000214451 |
HOGENOMi | CLU_104235_0_0_1 |
InParanoidi | Q7M730 |
OMAi | TWYFNST |
OrthoDBi | 1219179at2759 |
PhylomeDBi | Q7M730 |
TreeFami | TF331728 |
Enzyme and pathway databases
Reactomei | R-RNO-5576892, Phase 0 - rapid depolarisation |
Miscellaneous databases
PROi | PR:Q7M730 |
Gene expression databases
Bgeei | ENSRNOG00000026679, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | Q7M730, RN |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set IPR000920, Myelin_P0-rel |
PANTHERi | PTHR13869, PTHR13869, 1 hit |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SCN4B_RAT | |
Accessioni | Q7M730Primary (citable) accession number: Q7M730 Secondary accession number(s): Q80Z84 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | December 15, 2003 | |
Last modified: | December 2, 2020 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families