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Protein

Phosphatidylinositol-binding clathrin assembly protein

Gene

Picalm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. Plays a crucial role in fetal and adult hematopoiesis, and normal prenatal and postnatal growth and viability.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processEndocytosis

Enzyme and pathway databases

ReactomeiR-MMU-432722 Golgi Associated Vesicle Biogenesis
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-binding clathrin assembly protein
Alternative name(s):
Clathrin assembly lymphoid myeloid leukemia
Short name:
CALM
Gene namesi
Name:Picalm
Synonyms:Calm, Fit1
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2385902 Picalm

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001870632 – 660Phosphatidylinositol-binding clathrin assembly proteinAdd BLAST659

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei16PhosphoserineBy similarity1
Modified residuei20PhosphoserineBy similarity1
Cross-linki238Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei303PhosphoserineBy similarity1
Modified residuei315PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ7M6Y3
PeptideAtlasiQ7M6Y3
PRIDEiQ7M6Y3

PTM databases

iPTMnetiQ7M6Y3
PhosphoSitePlusiQ7M6Y3
SwissPalmiQ7M6Y3

Expressioni

Tissue specificityi

Skins and livers of 1-week-old mice.1 Publication

Gene expression databases

BgeeiENSMUSG00000039361 Expressed in 271 organ(s), highest expression level in humerus cartilage element
ExpressionAtlasiQ7M6Y3 baseline and differential
GenevisibleiQ7M6Y3 MM

Interactioni

Subunit structurei

Binds clathrin; involves primarily the C-terminal sequences, but the full-length protein is required for full binding capacity. Binds phosphatidylinositol 4,5- bisphosphate. Interacts with PIMREG; this interaction may change the subcellular location into the nucleus.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231417, 4 interactors
ELMiQ7M6Y3
IntActiQ7M6Y3, 5 interactors
MINTiQ7M6Y3
STRINGi10090.ENSMUSP00000051092

Structurei

3D structure databases

ProteinModelPortaliQ7M6Y3
SMRiQ7M6Y3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 145ENTHPROSITE-ProRule annotationCuratedAdd BLAST132

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 294Interaction with PIMREGBy similarityAdd BLAST74

Sequence similaritiesi

Belongs to the PICALM/SNAP91 family.Curated

Phylogenomic databases

eggNOGiKOG0251 Eukaryota
ENOG410XQ90 LUCA
GeneTreeiENSGT00390000008805
HOVERGENiHBG049391
InParanoidiQ7M6Y3
KOiK20044
OMAiPTGMMAY
OrthoDBiEOG091G0810
PhylomeDBiQ7M6Y3
TreeFamiTF314861

Family and domain databases

Gene3Di1.20.58.150, 1 hit
1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR011417 ANTH_dom
IPR014712 Clathrin_AP_dom2
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR030412 PICALM
PANTHERiPTHR22951:SF16 PTHR22951:SF16, 1 hit
PfamiView protein in Pfam
PF07651 ANTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit

Sequences (6+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 6 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1Curated (identifier: Q7M6Y3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN
60 70 80 90 100
EMNVNIPQLA DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR
110 120 130 140 150
NTLFNLSNFL DKSGLQGYDM STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR
160 170 180 190 200
GADGVMRTMN TEKLLKTVPI IQNQMDALLD FNVNSNELTN GVINAAFMLL
210 220 230 240 250
FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF LTRMTRISEF
260 270 280 290 300
LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT
310 320 330 340 350
TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP
360 370 380 390 400
HTSLTTAASP VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT
410 420 430 440 450
FHPSVHAMSA APQGASTWGD PFSATLDAVE DAIPSLNPFL TKSSGDVHLP
460 470 480 490 500
IASDVSTFTT RTPTHEMFVG FSPSPVAQPH SSAGLNVDFE SVFGNKSTNV
510 520 530 540 550
AVDSGGFDEL GGLLKPTVAS QNQSLPVAKL PPNKLVSDDL DSSLANLVGN
560 570 580 590 600
LGIGNGTTKN DVSWSQPGEK KLTGGSNWQP KVAPTTAWSA ATMNGMHFPQ
610 620 630 640 650
YAPPVMAYPA TTPTGMIGYG IPPQMGSVPV MTQPTLIYSQ PVMRPPNPFG
660
PVSGAQIQFM
Length:660
Mass (Da):71,543
Last modified:December 15, 2003 - v1
Checksum:i7FB206508281BCDF
GO
Isoform 2Curated (identifier: Q7M6Y3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     420-469: Missing.
     506-510: Missing.
     594-601: Missing.

Show »
Length:597
Mass (Da):64,648
Checksum:i825D7F7B7154DD8A
GO
Isoform 3Curated (identifier: Q7M6Y3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     420-469: Missing.
     506-510: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:605
Mass (Da):65,623
Checksum:iC365144C975C2B50
GO
Isoform 4Curated (identifier: Q7M6Y3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     420-469: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:610
Mass (Da):66,184
Checksum:i86E8189B6C05F005
GO
Isoform 5Curated (identifier: Q7M6Y3-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     506-510: Missing.

Show »
Length:655
Mass (Da):70,982
Checksum:iF729CEF5D3D82781
GO
Isoform 6Curated (identifier: Q7M6Y3-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     594-601: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:652
Mass (Da):70,568
Checksum:i0CEEEEA9225CC446
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A140LHQ8A0A140LHQ8_MOUSE
Phosphatidylinositol-binding clathr...
Picalm
531Annotation score:
A0A140LHG9A0A140LHG9_MOUSE
Phosphatidylinositol-binding clathr...
Picalm
85Annotation score:
A0A1L1SUR7A0A1L1SUR7_MOUSE
Phosphatidylinositol-binding clathr...
Picalm
404Annotation score:

Sequence cautioni

The sequence BAC39454 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti522N → S in AAH21491 (PubMed:15489334).Curated1
Sequence conflicti522N → S in AAH23843 (PubMed:15489334).Curated1
Sequence conflicti522N → S in AAH25566 (PubMed:15489334).Curated1
Sequence conflicti522N → S in AAH57683 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050684420 – 469Missing in isoform 2, isoform 3 and isoform 4. 2 PublicationsAdd BLAST50
Alternative sequenceiVSP_050685506 – 510Missing in isoform 2, isoform 3 and isoform 5. 2 Publications5
Alternative sequenceiVSP_050686594 – 601Missing in isoform 2 and isoform 6. 2 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY206701 mRNA Translation: AAO17153.1
BK001028 mRNA Translation: DAA01470.1
BC011470 mRNA Translation: AAH11470.1
BC021491 mRNA Translation: AAH21491.1
BC023843 mRNA Translation: AAH23843.1
BC025566 mRNA Translation: AAH25566.1
BC027116 mRNA Translation: AAH27116.1
BC057683 mRNA Translation: AAH57683.1
AK085472 mRNA Translation: BAC39454.2 Different initiation.
AK162360 mRNA Translation: BAE36872.1
CCDSiCCDS52307.1 [Q7M6Y3-1]
CCDS85334.1 [Q7M6Y3-6]
CCDS85335.1 [Q7M6Y3-5]
CCDS85336.1 [Q7M6Y3-4]
CCDS85337.1 [Q7M6Y3-3]
CCDS85338.1 [Q7M6Y3-2]
RefSeqiNP_001239449.1, NM_001252520.1 [Q7M6Y3-5]
NP_001239450.1, NM_001252521.1 [Q7M6Y3-6]
NP_001239451.1, NM_001252522.1 [Q7M6Y3-4]
NP_001239452.1, NM_001252523.1 [Q7M6Y3-3]
NP_001239453.1, NM_001252524.1 [Q7M6Y3-2]
NP_666306.2, NM_146194.4 [Q7M6Y3-1]
UniGeneiMm.235175
Mm.393563

Genome annotation databases

EnsembliENSMUST00000049537; ENSMUSP00000051092; ENSMUSG00000039361 [Q7M6Y3-5]
ENSMUST00000207225; ENSMUSP00000146659; ENSMUSG00000039361 [Q7M6Y3-2]
ENSMUST00000207484; ENSMUSP00000146501; ENSMUSG00000039361 [Q7M6Y3-1]
ENSMUST00000208730; ENSMUSP00000146541; ENSMUSG00000039361 [Q7M6Y3-3]
ENSMUST00000208742; ENSMUSP00000147016; ENSMUSG00000039361 [Q7M6Y3-6]
ENSMUST00000209068; ENSMUSP00000146386; ENSMUSG00000039361 [Q7M6Y3-4]
GeneIDi233489
KEGGimmu:233489
UCSCiuc009igq.2 mouse [Q7M6Y3-1]
uc009igr.2 mouse [Q7M6Y3-5]
uc009igs.2 mouse [Q7M6Y3-6]
uc009igt.2 mouse [Q7M6Y3-4]
uc009igu.2 mouse [Q7M6Y3-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY206701 mRNA Translation: AAO17153.1
BK001028 mRNA Translation: DAA01470.1
BC011470 mRNA Translation: AAH11470.1
BC021491 mRNA Translation: AAH21491.1
BC023843 mRNA Translation: AAH23843.1
BC025566 mRNA Translation: AAH25566.1
BC027116 mRNA Translation: AAH27116.1
BC057683 mRNA Translation: AAH57683.1
AK085472 mRNA Translation: BAC39454.2 Different initiation.
AK162360 mRNA Translation: BAE36872.1
CCDSiCCDS52307.1 [Q7M6Y3-1]
CCDS85334.1 [Q7M6Y3-6]
CCDS85335.1 [Q7M6Y3-5]
CCDS85336.1 [Q7M6Y3-4]
CCDS85337.1 [Q7M6Y3-3]
CCDS85338.1 [Q7M6Y3-2]
RefSeqiNP_001239449.1, NM_001252520.1 [Q7M6Y3-5]
NP_001239450.1, NM_001252521.1 [Q7M6Y3-6]
NP_001239451.1, NM_001252522.1 [Q7M6Y3-4]
NP_001239452.1, NM_001252523.1 [Q7M6Y3-3]
NP_001239453.1, NM_001252524.1 [Q7M6Y3-2]
NP_666306.2, NM_146194.4 [Q7M6Y3-1]
UniGeneiMm.235175
Mm.393563

3D structure databases

ProteinModelPortaliQ7M6Y3
SMRiQ7M6Y3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231417, 4 interactors
ELMiQ7M6Y3
IntActiQ7M6Y3, 5 interactors
MINTiQ7M6Y3
STRINGi10090.ENSMUSP00000051092

PTM databases

iPTMnetiQ7M6Y3
PhosphoSitePlusiQ7M6Y3
SwissPalmiQ7M6Y3

Proteomic databases

PaxDbiQ7M6Y3
PeptideAtlasiQ7M6Y3
PRIDEiQ7M6Y3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049537; ENSMUSP00000051092; ENSMUSG00000039361 [Q7M6Y3-5]
ENSMUST00000207225; ENSMUSP00000146659; ENSMUSG00000039361 [Q7M6Y3-2]
ENSMUST00000207484; ENSMUSP00000146501; ENSMUSG00000039361 [Q7M6Y3-1]
ENSMUST00000208730; ENSMUSP00000146541; ENSMUSG00000039361 [Q7M6Y3-3]
ENSMUST00000208742; ENSMUSP00000147016; ENSMUSG00000039361 [Q7M6Y3-6]
ENSMUST00000209068; ENSMUSP00000146386; ENSMUSG00000039361 [Q7M6Y3-4]
GeneIDi233489
KEGGimmu:233489
UCSCiuc009igq.2 mouse [Q7M6Y3-1]
uc009igr.2 mouse [Q7M6Y3-5]
uc009igs.2 mouse [Q7M6Y3-6]
uc009igt.2 mouse [Q7M6Y3-4]
uc009igu.2 mouse [Q7M6Y3-3]

Organism-specific databases

CTDi8301
MGIiMGI:2385902 Picalm

Phylogenomic databases

eggNOGiKOG0251 Eukaryota
ENOG410XQ90 LUCA
GeneTreeiENSGT00390000008805
HOVERGENiHBG049391
InParanoidiQ7M6Y3
KOiK20044
OMAiPTGMMAY
OrthoDBiEOG091G0810
PhylomeDBiQ7M6Y3
TreeFamiTF314861

Enzyme and pathway databases

ReactomeiR-MMU-432722 Golgi Associated Vesicle Biogenesis
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

ChiTaRSiPicalm mouse
PROiPR:Q7M6Y3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039361 Expressed in 271 organ(s), highest expression level in humerus cartilage element
ExpressionAtlasiQ7M6Y3 baseline and differential
GenevisibleiQ7M6Y3 MM

Family and domain databases

Gene3Di1.20.58.150, 1 hit
1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR011417 ANTH_dom
IPR014712 Clathrin_AP_dom2
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR030412 PICALM
PANTHERiPTHR22951:SF16 PTHR22951:SF16, 1 hit
PfamiView protein in Pfam
PF07651 ANTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPICAL_MOUSE
AccessioniPrimary (citable) accession number: Q7M6Y3
Secondary accession number(s): Q3TS04
, Q811P1, Q8BUF6, Q8CIH8, Q8R0A9, Q8R3E1, Q8VDN5, Q921L0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: December 15, 2003
Last modified: November 7, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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