Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Carbohydrate sulfotransferase 15

Gene

CHST15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.2 Publications

Catalytic activityi

3-phospho-5-adenylyl sulfate + [dermatan]-4-O-sulfo-N-acetylgalactosamine = adenosine 3',5'-bisphosphate + [dermatan]-4,6-di-O-sulfo-N-acetyl-D-galactosamine.1 Publication
3-phospho-5-adenylyl sulfate + [chondroitin]-4-O-sulfo-N-acetylgalactosamine = adenosine 3',5'-bisphosphate + [chondroitin]-4,6-di-O-sulfo-N-acetyl-D-galactosamine.1 Publication

Cofactori

Protein has several cofactor binding sites:

Activity regulationi

Inhibited by phenyl beta-GalNAc(4,6-SO4).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei392PAPSBy similarity1
Binding sitei400PAPSBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi263 – 267PAPSBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BioCyciMetaCyc:HS11694-MONOMER
BRENDAi2.8.2.33 2681
ReactomeiR-HSA-2022870 Chondroitin sulfate biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 15 (EC:2.8.2.33)
Alternative name(s):
B-cell RAG-associated gene protein
Short name:
hBRAG
N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase
Short name:
GalNAc4S-6ST
Gene namesi
Name:CHST15
Synonyms:BRAG, GALNAC4S6ST, KIAA0598
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000182022.17
HGNCiHGNC:18137 CHST15
MIMi608277 gene
neXtProtiNX_Q7LFX5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 80CytoplasmicSequence analysisAdd BLAST80
Transmembranei81 – 101Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini102 – 561LumenalSequence analysisAdd BLAST460

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi51363
OpenTargetsiENSG00000182022
PharmGKBiPA165548385

Polymorphism and mutation databases

DMDMi74749920

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002256231 – 561Carbohydrate sulfotransferase 15Add BLAST561

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi364N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ7LFX5
PeptideAtlasiQ7LFX5
PRIDEiQ7LFX5
ProteomicsDBi68858
68859 [Q7LFX5-2]

PTM databases

iPTMnetiQ7LFX5
PhosphoSitePlusiQ7LFX5

Expressioni

Tissue specificityi

Expressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1.1 Publication

Gene expression databases

BgeeiENSG00000182022 Expressed in 220 organ(s), highest expression level in blood
ExpressionAtlasiQ7LFX5 baseline and differential
GenevisibleiQ7LFX5 HS

Organism-specific databases

HPAiHPA017584

Interactioni

Subunit structurei

Homodimer; disulfide-linked (Potential). The relevance of homodimerization is however unsure. May interact with phosphorylated proteins in resting B-cells, including HCK.Curated1 Publication

Protein-protein interaction databases

BioGridi119498, 25 interactors
IntActiQ7LFX5, 20 interactors
STRINGi9606.ENSP00000333947

Structurei

3D structure databases

ProteinModelPortaliQ7LFX5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IISS Eukaryota
ENOG410XSMU LUCA
GeneTreeiENSGT00390000004719
HOGENOMiHOG000154435
InParanoidiQ7LFX5
KOiK08106
OMAiHASNVKY
OrthoDBiEOG091G039B
PhylomeDBiQ7LFX5
TreeFamiTF333516

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
SUPFAMiSSF52540 SSF52540, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7LFX5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRHCINCCIQ LLPDGAHKQQ VNCQGGPHHG HQACPTCKGE NKILFRVDSK
60 70 80 90 100
QMNLLAVLEV RTEGNENWGG FLRFKKGKRC SLVFGLIIMT LVMASYILSG
110 120 130 140 150
AHQELLISSP FHYGGFPSNP SLMDSENPSD TKEHHHQSSV NNISYMKDYP
160 170 180 190 200
SIKLIINSIT TRIEFTTRQL PDLEDLKKQE LHMFSVIPNK FLPNSKSPCW
210 220 230 240 250
YEEFSGQNTT DPYLTNSYVL YSKRFRSTFD ALRKAFWGHL AHAHGKHFRL
260 270 280 290 300
RCLPHFYIIG QPKCGTTDLY DRLRLHPEVK FSAIKEPHWW TRKRFGIVRL
310 320 330 340 350
RDGLRDRYPV EDYLDLFDLA AHQIHQGLQA SSAKEQSKMN TIIIGEASAS
360 370 380 390 400
TMWDNNAWTF FYDNSTDGEP PFLTQDFIHA FQPNARLIVM LRDPVERLYS
410 420 430 440 450
DYLYFASSNK SADDFHEKVT EALQLFENCM LDYSLRACVY NNTLNNAMPV
460 470 480 490 500
RLQVGLYAVY LLDWLSVFDK QQFLILRLED HASNVKYTMH KVFQFLNLGP
510 520 530 540 550
LSEKQEALMT KSPASNARRP EDRNLGPMWP ITQKILRDFY RPFNARLAQV
560
LADEAFAWKT T
Length:561
Mass (Da):64,926
Last modified:July 5, 2004 - v1
Checksum:i74643A7CFF7F242D
GO
Isoform 2 (identifier: Q7LFX5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     451-561: RLQVGLYAVY...ADEAFAWKTT → CTPPPRTPRA...PLFMDVKAEN

Note: No experimental confirmation available.
Show »
Length:506
Mass (Da):58,109
Checksum:iFD022040B4DB2920
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0D9SFZ3A0A0D9SFZ3_HUMAN
Carbohydrate sulfotransferase 15
CHST15
66Annotation score:

Sequence cautioni

The sequence AAC71691 differs from that shown. Reason: Frameshift at positions 180, 223, 489 and 537.Curated
The sequence AAH50540 differs from that shown. Reason: Frameshift at position 511.Curated
The sequence BAA25524 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017387451 – 561RLQVG…AWKTT → CTPPPRTPRAGPWQKELVCC YYASGIVGLRFSIGTERSVL MCKCCSPLFMDVKAEN in isoform 2. 1 PublicationAdd BLAST111

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026477 mRNA Translation: AAC71691.1 Frameshift.
AB025341 Genomic DNA Translation: BAA83686.1
AB062423 mRNA Translation: BAB72145.1
AB011170 mRNA Translation: BAA25524.2 Different initiation.
CR749804 mRNA Translation: CAH18664.1
AL683842 Genomic DNA No translation available.
BC027908 mRNA Translation: AAH27908.1
BC050540 mRNA Translation: AAH50540.1 Frameshift.
BC075813 mRNA Translation: AAH75813.1
CCDSiCCDS55731.1 [Q7LFX5-2]
CCDS7638.1 [Q7LFX5-1]
RefSeqiNP_001257693.1, NM_001270764.1 [Q7LFX5-1]
NP_001257694.1, NM_001270765.1 [Q7LFX5-2]
NP_056976.2, NM_015892.4 [Q7LFX5-1]
XP_005269948.1, XM_005269891.3 [Q7LFX5-1]
XP_006717954.1, XM_006717891.3 [Q7LFX5-1]
XP_016871808.1, XM_017016319.1 [Q7LFX5-1]
UniGeneiHs.287537
Hs.745222

Genome annotation databases

EnsembliENST00000346248; ENSP00000333947; ENSG00000182022 [Q7LFX5-1]
ENST00000435907; ENSP00000402394; ENSG00000182022 [Q7LFX5-1]
ENST00000628426; ENSP00000485905; ENSG00000182022 [Q7LFX5-2]
GeneIDi51363
KEGGihsa:51363
UCSCiuc001lhm.5 human [Q7LFX5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026477 mRNA Translation: AAC71691.1 Frameshift.
AB025341 Genomic DNA Translation: BAA83686.1
AB062423 mRNA Translation: BAB72145.1
AB011170 mRNA Translation: BAA25524.2 Different initiation.
CR749804 mRNA Translation: CAH18664.1
AL683842 Genomic DNA No translation available.
BC027908 mRNA Translation: AAH27908.1
BC050540 mRNA Translation: AAH50540.1 Frameshift.
BC075813 mRNA Translation: AAH75813.1
CCDSiCCDS55731.1 [Q7LFX5-2]
CCDS7638.1 [Q7LFX5-1]
RefSeqiNP_001257693.1, NM_001270764.1 [Q7LFX5-1]
NP_001257694.1, NM_001270765.1 [Q7LFX5-2]
NP_056976.2, NM_015892.4 [Q7LFX5-1]
XP_005269948.1, XM_005269891.3 [Q7LFX5-1]
XP_006717954.1, XM_006717891.3 [Q7LFX5-1]
XP_016871808.1, XM_017016319.1 [Q7LFX5-1]
UniGeneiHs.287537
Hs.745222

3D structure databases

ProteinModelPortaliQ7LFX5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119498, 25 interactors
IntActiQ7LFX5, 20 interactors
STRINGi9606.ENSP00000333947

PTM databases

iPTMnetiQ7LFX5
PhosphoSitePlusiQ7LFX5

Polymorphism and mutation databases

DMDMi74749920

Proteomic databases

PaxDbiQ7LFX5
PeptideAtlasiQ7LFX5
PRIDEiQ7LFX5
ProteomicsDBi68858
68859 [Q7LFX5-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000346248; ENSP00000333947; ENSG00000182022 [Q7LFX5-1]
ENST00000435907; ENSP00000402394; ENSG00000182022 [Q7LFX5-1]
ENST00000628426; ENSP00000485905; ENSG00000182022 [Q7LFX5-2]
GeneIDi51363
KEGGihsa:51363
UCSCiuc001lhm.5 human [Q7LFX5-1]

Organism-specific databases

CTDi51363
DisGeNETi51363
EuPathDBiHostDB:ENSG00000182022.17
GeneCardsiCHST15
HGNCiHGNC:18137 CHST15
HPAiHPA017584
MIMi608277 gene
neXtProtiNX_Q7LFX5
OpenTargetsiENSG00000182022
PharmGKBiPA165548385
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IISS Eukaryota
ENOG410XSMU LUCA
GeneTreeiENSGT00390000004719
HOGENOMiHOG000154435
InParanoidiQ7LFX5
KOiK08106
OMAiHASNVKY
OrthoDBiEOG091G039B
PhylomeDBiQ7LFX5
TreeFamiTF333516

Enzyme and pathway databases

BioCyciMetaCyc:HS11694-MONOMER
BRENDAi2.8.2.33 2681
ReactomeiR-HSA-2022870 Chondroitin sulfate biosynthesis

Miscellaneous databases

ChiTaRSiCHST15 human
GeneWikiiGALNAC4S-6ST
GenomeRNAii51363
PROiPR:Q7LFX5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000182022 Expressed in 220 organ(s), highest expression level in blood
ExpressionAtlasiQ7LFX5 baseline and differential
GenevisibleiQ7LFX5 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHSTF_HUMAN
AccessioniPrimary (citable) accession number: Q7LFX5
Secondary accession number(s): O60338, O60474, Q86VM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 5, 2004
Last modified: September 12, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again