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Protein

Tubulinyl-Tyr carboxypeptidase 1

Gene

VASH1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tyrosine carboxypeptidase that removes the C-terminal tyrosine residue of alpha-tubulin, thereby regulating microtubule dynamics and function (PubMed:29146869). Acts as an angiogenesis inhibitor: inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis (PubMed:15467828, PubMed:16488400, PubMed:16707096, PubMed:19204325). This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts (PubMed:15467828, PubMed:16488400, PubMed:16707096).5 Publications

Catalytic activityi

Cleavage of the -Glu-|-Tyr bond to release the C-terminal tyrosine residue from the native tyrosinated tubulin. Inactive on Z-Glu-Tyr.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1691 Publication1 Publication1
Active sitei2041 Publication1
Active sitei2211 Publication1

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • metallocarboxypeptidase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease
Biological processCell cycle, Growth arrest

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulinyl-Tyr carboxypeptidase 1Curated (EC:3.4.17.171 Publication)
Alternative name(s):
Tubulin carboxypeptidase 11 Publication
Tyrosine carboxypeptidase 1Curated
Short name:
TTCP 1Curated
Vasohibin-11 Publication
Gene namesi
Name:VASH1Imported
Synonyms:KIAA10361 Publication, VASH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000071246.10
HGNCiHGNC:19964 VASH1
MIMi609011 gene
neXtProtiNX_Q7L8A9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi29R → A: Disappearance of 42 kDa processed form. 1 Publication1
Mutagenesisi76R → A: Disappearance of 36, 32 and 27 kDa processed forms. 1 Publication1
Mutagenesisi169C → A: Abolished tyrosine carboxypeptidase activity. 1 Publication1

Organism-specific databases

DisGeNETi22846
OpenTargetsiENSG00000071246
PharmGKBiPA134941450

Polymorphism and mutation databases

BioMutaiVASH1
DMDMi62511163

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001899801 – 365Tubulinyl-Tyr carboxypeptidase 1Add BLAST365

Post-translational modificationi

2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic cleavage (PubMed:16488400). The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems to accumulate within the cells or pericellular milieu (PubMed:16488400). Polypeptide consisting of Met-77 to Arg-318 may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the 36 kDa form (PubMed:16488400).1 Publication
Ubiquitinated in vitro.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei29 – 30Cleavage1 Publication2
Sitei76 – 77Cleavage1 Publication2

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ7L8A9
PeptideAtlasiQ7L8A9
PRIDEiQ7L8A9
ProteomicsDBi68834
68835 [Q7L8A9-2]

PTM databases

iPTMnetiQ7L8A9
PhosphoSitePlusiQ7L8A9

Expressioni

Tissue specificityi

Preferentially expressed in endothelial cells (PubMed:15467828, PubMed:16707096). Highly expressed in fetal organs (PubMed:15467828). Expressed in brain and placenta, and at lower level in heart and kidney (PubMed:15467828). Highly detected in microvessels endothelial cells of atherosclerotic lesions (PubMed:16707096).2 Publications

Inductioni

By VEGF.2 Publications

Gene expression databases

BgeeiENSG00000071246 Expressed in 136 organ(s), highest expression level in substantia nigra
CleanExiHS_VASH1
ExpressionAtlasiQ7L8A9 baseline and differential
GenevisibleiQ7L8A9 HS

Organism-specific databases

HPAiHPA000653

Interactioni

Subunit structurei

Interacts with SVBP; interaction enhances VASH1 tyrosine carboxypeptidase activity.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DHPSP493663EBI-10256546,EBI-741925

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116518, 11 interactors
IntActiQ7L8A9, 1 interactor
STRINGi9606.ENSP00000167106

Structurei

3D structure databases

ProteinModelPortaliQ7L8A9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni319 – 365Involved in heparin-binding and antiangiogenic activity1 PublicationAdd BLAST47

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFRP Eukaryota
ENOG410ZG80 LUCA
GeneTreeiENSGT00390000012703
HOGENOMiHOG000007477
HOVERGENiHBG079337
InParanoidiQ7L8A9
OMAiIEWKHSI
OrthoDBiEOG091G0CSJ
PhylomeDBiQ7L8A9
TreeFamiTF329370

Family and domain databases

InterProiView protein in InterPro
IPR028131 Vasohibin
IPR028132 Vasohibin-1
PANTHERiPTHR15750 PTHR15750, 1 hit
PTHR15750:SF2 PTHR15750:SF2, 1 hit
PfamiView protein in Pfam
PF14822 Vasohibin, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7L8A9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPGGKKVAGG GSSGATPTSA AATAPSGVRR LETSEGTSAQ RDEEPEEEGE
60 70 80 90 100
EDLRDGGVPF FVNRGGLPVD EATWERMWKH VAKIHPDGEK VAQRIRGATD
110 120 130 140 150
LPKIPIPSVP TFQPSTPVPE RLEAVQRYIR ELQYNHTGTQ FFEIKKSRPL
160 170 180 190 200
TGLMDLAKEM TKEALPIKCL EAVILGIYLT NSMPTLERFP ISFKTYFSGN
210 220 230 240 250
YFRHIVLGVN FAGRYGALGM SRREDLMYKP PAFRTLSELV LDFEAAYGRC
260 270 280 290 300
WHVLKKVKLG QSVSHDPHSV EQIEWKHSVL DVERLGRDDF RKELERHARD
310 320 330 340 350
MRLKIGKGTG PPSPTKDRKK DVSSPQRAQS SPHRRNSRSE RRPSGDKKTS
360
EPKAMPDLNG YQIRV
Length:365
Mass (Da):40,957
Last modified:July 5, 2004 - v1
Checksum:iCEE39875FA9DA70B
GO
Isoform 2 (identifier: Q7L8A9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     177-204: IYLTNSMPTLERFPISFKTYFSGNYFRH → MYPSSPEGEGSGLLWASASCSESEGGVG
     205-365: Missing.

Show »
Length:204
Mass (Da):21,845
Checksum:iCE4652CF401BBA85
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V4N9G3V4N9_HUMAN
Tubulinyl-Tyr carboxypeptidase 1
VASH1
91Annotation score:

Sequence cautioni

The sequence AAD44361 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAA82988 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013324177 – 204IYLTN…NYFRH → MYPSSPEGEGSGLLWASASC SESEGGVG in isoform 2. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_013325205 – 365Missing in isoform 2. 1 PublicationAdd BLAST161

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028959 mRNA Translation: BAA82988.2 Different initiation.
AL832588 mRNA Translation: CAD89941.1
AC007376 Genomic DNA Translation: AAF02829.1
AF111169 Genomic DNA Translation: AAD44361.1 Sequence problems.
BC009031 mRNA Translation: AAH09031.1
BC051896 mRNA Translation: AAH51896.1
CCDSiCCDS9851.1 [Q7L8A9-1]
RefSeqiNP_055724.1, NM_014909.4 [Q7L8A9-1]
UniGeneiHs.525479

Genome annotation databases

EnsembliENST00000167106; ENSP00000167106; ENSG00000071246 [Q7L8A9-1]
ENST00000554237; ENSP00000451613; ENSG00000071246 [Q7L8A9-2]
GeneIDi22846
KEGGihsa:22846
UCSCiuc001xss.4 human [Q7L8A9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028959 mRNA Translation: BAA82988.2 Different initiation.
AL832588 mRNA Translation: CAD89941.1
AC007376 Genomic DNA Translation: AAF02829.1
AF111169 Genomic DNA Translation: AAD44361.1 Sequence problems.
BC009031 mRNA Translation: AAH09031.1
BC051896 mRNA Translation: AAH51896.1
CCDSiCCDS9851.1 [Q7L8A9-1]
RefSeqiNP_055724.1, NM_014909.4 [Q7L8A9-1]
UniGeneiHs.525479

3D structure databases

ProteinModelPortaliQ7L8A9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116518, 11 interactors
IntActiQ7L8A9, 1 interactor
STRINGi9606.ENSP00000167106

PTM databases

iPTMnetiQ7L8A9
PhosphoSitePlusiQ7L8A9

Polymorphism and mutation databases

BioMutaiVASH1
DMDMi62511163

Proteomic databases

PaxDbiQ7L8A9
PeptideAtlasiQ7L8A9
PRIDEiQ7L8A9
ProteomicsDBi68834
68835 [Q7L8A9-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000167106; ENSP00000167106; ENSG00000071246 [Q7L8A9-1]
ENST00000554237; ENSP00000451613; ENSG00000071246 [Q7L8A9-2]
GeneIDi22846
KEGGihsa:22846
UCSCiuc001xss.4 human [Q7L8A9-1]

Organism-specific databases

CTDi22846
DisGeNETi22846
EuPathDBiHostDB:ENSG00000071246.10
GeneCardsiVASH1
HGNCiHGNC:19964 VASH1
HPAiHPA000653
MIMi609011 gene
neXtProtiNX_Q7L8A9
OpenTargetsiENSG00000071246
PharmGKBiPA134941450
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFRP Eukaryota
ENOG410ZG80 LUCA
GeneTreeiENSGT00390000012703
HOGENOMiHOG000007477
HOVERGENiHBG079337
InParanoidiQ7L8A9
OMAiIEWKHSI
OrthoDBiEOG091G0CSJ
PhylomeDBiQ7L8A9
TreeFamiTF329370

Miscellaneous databases

ChiTaRSiVASH1 human
GeneWikiiVASH1
GenomeRNAii22846
PROiPR:Q7L8A9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000071246 Expressed in 136 organ(s), highest expression level in substantia nigra
CleanExiHS_VASH1
ExpressionAtlasiQ7L8A9 baseline and differential
GenevisibleiQ7L8A9 HS

Family and domain databases

InterProiView protein in InterPro
IPR028131 Vasohibin
IPR028132 Vasohibin-1
PANTHERiPTHR15750 PTHR15750, 1 hit
PTHR15750:SF2 PTHR15750:SF2, 1 hit
PfamiView protein in Pfam
PF14822 Vasohibin, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVASH1_HUMAN
AccessioniPrimary (citable) accession number: Q7L8A9
Secondary accession number(s): Q96H02, Q9UBF4, Q9Y629
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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