UniProtKB - Q7L5Y9 (MAEA_HUMAN)
E3 ubiquitin-protein transferase MAEA
MAEA
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 314 | Essential for ubiquitin ligase activityBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 314 – 381 | RING-Gid-typePROSITE-ProRule annotationAdd BLAST | 68 |
GO - Molecular functioni
- actin binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: InterPro
GO - Biological processi
- cell adhesion Source: UniProtKB
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- erythrocyte maturation Source: UniProtKB-KW
- negative regulation of gluconeogenesis Source: InterPro
- negative regulation of myeloid cell apoptotic process Source: UniProtKB
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- regulation of mitotic cell cycle Source: UniProtKB
Keywordsi
Molecular function | Actin-binding, Transferase |
Biological process | Cell cycle, Cell division, Erythrocyte maturation, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q7L5Y9 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein transferase MAEA (EC:2.3.2.271 Publication)Alternative name(s): Cell proliferation-inducing gene 5 protein1 Publication Erythroblast macrophage protein1 Publication Human lung cancer oncogene 10 protein Short name: HLC-10 Macrophage erythroblast attacher P44EMLP1 Publication |
Gene namesi | Name:MAEA Synonyms:EMP2 Publications ORF Names:HLC10, PIG51 Publication |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:13731, MAEA |
MIMi | 606801, gene |
neXtProti | NX_Q7L5Y9 |
VEuPathDBi | HostDB:ENSG00000090316.15 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytoskeleton
- cytoskeleton 1 Publication
Plasma membrane
- Cell membrane 1 Publication
Nucleus
- nucleoplasm 3 Publications
- Nucleus matrix 1 Publication
Note: Detected in a nuclear, speckled-like pattern (PubMed:16510120). Localized with condensed chromatin at prophase; Detected in nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis (PubMed:16510120). Present in cytoplasm, nuclear matrix and at the cell surface in macrophages; predominantly nuclear in immature macrophages and predominantly detected at the cell surface in mature macrophages. Colocalizes with F-actin in macrophages (By similarity).By similarity1 Publication
Cytoskeleton
- actomyosin contractile ring Source: UniProtKB
- cytoskeleton Source: UniProtKB
- spindle Source: UniProtKB
Nucleus
- nuclear matrix Source: UniProtKB
- nucleoplasm Source: UniProtKB
- nucleus Source: UniProtKB
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- GID complex Source: GO_Central
- ubiquitin ligase complex Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 10296 |
OpenTargetsi | ENSG00000090316 |
PharmGKBi | PA30533 |
Miscellaneous databases
Pharosi | Q7L5Y9, Tbio |
Chemistry databases
DrugBanki | DB05389, Tetrachlorodecaoxide |
Genetic variation databases
BioMutai | MAEA |
DMDMi | 74754297 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000284936 | 1 – 396 | E3 ubiquitin-protein transferase MAEAAdd BLAST | 396 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 28 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q7L5Y9 |
jPOSTi | Q7L5Y9 |
MassIVEi | Q7L5Y9 |
MaxQBi | Q7L5Y9 |
PaxDbi | Q7L5Y9 |
PeptideAtlasi | Q7L5Y9 |
PRIDEi | Q7L5Y9 |
ProteomicsDBi | 68819 [Q7L5Y9-1] 68820 [Q7L5Y9-2] 68821 [Q7L5Y9-3] 68822 [Q7L5Y9-4] 68823 [Q7L5Y9-5] |
PTM databases
iPTMneti | Q7L5Y9 |
PhosphoSitePlusi | Q7L5Y9 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000090316, Expressed in blood and 238 other tissues |
ExpressionAtlasi | Q7L5Y9, baseline and differential |
Genevisiblei | Q7L5Y9, HS |
Organism-specific databases
HPAi | ENSG00000090316, Low tissue specificity |
Interactioni
Subunit structurei
Identified in the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5 (PubMed:17467196, PubMed:29911972). Within this complex, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, WDR26, and RANBP9 and/or RANBP10 form the catalytic core, while GID4, MKLN1, ARMC8 and YPEL5 have ancillary roles (PubMed:29911972).
Interacts with F-actin (PubMed:16510120).
3 PublicationsGO - Molecular functioni
- actin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 115584, 90 interactors |
CORUMi | Q7L5Y9 |
IntActi | Q7L5Y9, 39 interactors |
STRINGi | 9606.ENSP00000302830 |
Miscellaneous databases
RNActi | Q7L5Y9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 121 – 153 | LisHPROSITE-ProRule annotationAdd BLAST | 33 | |
Domaini | 159 – 216 | CTLHPROSITE-ProRule annotationAdd BLAST | 58 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 124 | Extracellular and involved in cell to cell contact1 PublicationAdd BLAST | 124 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 314 – 381 | RING-Gid-typePROSITE-ProRule annotationAdd BLAST | 68 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0396, Eukaryota |
GeneTreei | ENSGT00940000153203 |
HOGENOMi | CLU_027445_0_1_1 |
InParanoidi | Q7L5Y9 |
OrthoDBi | 1087488at2759 |
PhylomeDBi | Q7L5Y9 |
TreeFami | TF314273 |
Family and domain databases
InterProi | View protein in InterPro IPR013144, CRA_dom IPR024964, CTLH/CRA IPR006595, CTLH_C IPR027714, Fyv10/EMP IPR006594, LisH IPR044063, ZF_RING_GID |
PANTHERi | PTHR12170:SF2, PTHR12170:SF2, 1 hit |
Pfami | View protein in Pfam PF10607, CLTH, 1 hit |
SMARTi | View protein in SMART SM00757, CRA, 1 hit SM00668, CTLH, 1 hit SM00667, LisH, 1 hit |
PROSITEi | View protein in PROSITE PS50897, CTLH, 1 hit PS50896, LISH, 1 hit PS51867, ZF_RING_GID, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAVQESAAQL SMTLKVQEYP TLKVPYETLN KRFRAAQKNI DRETSHVTMV
60 70 80 90 100
VAELEKTLSG CPAVDSVVSL LDGVVEKLSV LKRKAVESIQ AEDESAKLCK
110 120 130 140 150
RRIEHLKEHS SDQPAAASVW KRKRMDRMMV EHLLRCGYYN TAVKLARQSG
160 170 180 190 200
IEDLVNIEMF LTAKEVEESL ERRETATCLA WCHDNKSRLR KMKSCLEFSL
210 220 230 240 250
RIQEFIELIR QNKRLDAVRH ARKHFSQAEG SQLDEVRQAM GMLAFPPDTH
260 270 280 290 300
ISPYKDLLDP ARWRMLIQQF RYDNYRLHQL GNNSVFTLTL QAGLSAIKTP
310 320 330 340 350
QCYKEDGSSK SPDCPVCSRS LNKLAQPLPM AHCANSRLVC KISGDVMNEN
360 370 380 390
NPPMMLPNGY VYGYNSLLSI RQDDKVVCPR TKEVFHFSQA EKVYIM
The sequence of this isoform differs from the canonical sequence as follows:
153-245: DLVNIEMFLT...VRQAMGMLAF → TCKKALQPSR...LQGGRQLQEP
246-396: Missing.
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE7ESC7 | E7ESC7_HUMAN | E3 ubiquitin-protein transferase MA... | MAEA | 434 | Annotation score: | ||
B4DVN3 | B4DVN3_HUMAN | E3 ubiquitin-protein transferase MA... | MAEA | 395 | Annotation score: | ||
B4DQT1 | B4DQT1_HUMAN | E3 ubiquitin-protein transferase MA... | MAEA | 348 | Annotation score: | ||
D6RIB6 | D6RIB6_HUMAN | E3 ubiquitin-protein transferase MA... | MAEA | 328 | Annotation score: | ||
D6REW7 | D6REW7_HUMAN | E3 ubiquitin-protein transferase MA... | MAEA | 274 | Annotation score: | ||
D6RID6 | D6RID6_HUMAN | E3 ubiquitin-protein transferase MA... | MAEA | 115 | Annotation score: | ||
D6RDW4 | D6RDW4_HUMAN | E3 ubiquitin-protein transferase MA... | MAEA | 245 | Annotation score: | ||
D6RE80 | D6RE80_HUMAN | E3 ubiquitin-protein transferase MA... | MAEA | 183 | Annotation score: | ||
D6R8Z3 | D6R8Z3_HUMAN | E3 ubiquitin-protein transferase MA... | MAEA | 67 | Annotation score: | ||
D6RA86 | D6RA86_HUMAN | E3 ubiquitin-protein transferase MA... | MAEA | 107 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 32 | R → C in AAP74806 (Ref. 1) Curated | 1 | |
Sequence conflicti | 56 | K → R in AAC67543 (PubMed:9763581).Curated | 1 | |
Sequence conflicti | 101 | R → L in AAC67543 (PubMed:9763581).Curated | 1 | |
Sequence conflicti | 123 | K → R in BAA91499 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 313 | D → V in AAC67543 (PubMed:9763581).Curated | 1 | |
Sequence conflicti | 327 | P → R in AAC67543 (PubMed:9763581).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051150 | 34 | R → C. Corresponds to variant dbSNP:rs34082974Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024784 | 152 – 232 | EDLVN…AEGSQ → GTCKKALQPSRREPAGRGAP GHGHAGLPARHAHLPVQGPS GPCTVADADPAVPVRQLPTT PAGKQFCVHPHPAGWPLSHQ D in isoform 4. 1 PublicationAdd BLAST | 81 | |
Alternative sequenceiVSP_024785 | 153 – 245 | DLVNI…GMLAF → TCKKALQPSRREPAGRGAPG HGHAGLPARHAHLPVQGPSG PCTVADADPAVPVRQLPTTP AGKQFCVHPHPAGRPLSHQD TTVLQGGRQLQEP in isoform 5. 1 PublicationAdd BLAST | 93 | |
Alternative sequenceiVSP_024786 | 153 – 193 | Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST | 41 | |
Alternative sequenceiVSP_024788 | 233 – 300 | Missing in isoform 4. 1 PublicationAdd BLAST | 68 | |
Alternative sequenceiVSP_024789 | 246 – 396 | Missing in isoform 5. 1 PublicationAdd BLAST | 151 | |
Alternative sequenceiVSP_024790 | 265 | M → TCTVAD in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY236486 mRNA Translation: AAP74806.1 AK001088 mRNA Translation: BAA91499.1 AK128302 mRNA No translation available. AK022515 mRNA Translation: BAB14072.1 AK022586 mRNA Translation: BAB14113.1 BC001225 mRNA Translation: AAH01225.2 BC006470 mRNA Translation: AAH06470.2 AF084928 mRNA Translation: AAC67543.1 Sequence problems. AY189687 mRNA Translation: AAO85220.1 Different initiation. BT006957 mRNA Translation: AAP35603.1 |
CCDSi | CCDS33936.1 [Q7L5Y9-1] CCDS33937.1 [Q7L5Y9-3] CCDS77887.1 [Q7L5Y9-4] |
RefSeqi | NP_001017405.1, NM_001017405.2 [Q7L5Y9-1] NP_001284360.1, NM_001297431.1 NP_005873.2, NM_005882.4 [Q7L5Y9-3] |
Genome annotation databases
Ensembli | ENST00000264750; ENSP00000264750; ENSG00000090316 [Q7L5Y9-3] ENST00000303400; ENSP00000302830; ENSG00000090316 [Q7L5Y9-1] |
GeneIDi | 10296 |
KEGGi | hsa:10296 |
UCSCi | uc003gda.4, human [Q7L5Y9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY236486 mRNA Translation: AAP74806.1 AK001088 mRNA Translation: BAA91499.1 AK128302 mRNA No translation available. AK022515 mRNA Translation: BAB14072.1 AK022586 mRNA Translation: BAB14113.1 BC001225 mRNA Translation: AAH01225.2 BC006470 mRNA Translation: AAH06470.2 AF084928 mRNA Translation: AAC67543.1 Sequence problems. AY189687 mRNA Translation: AAO85220.1 Different initiation. BT006957 mRNA Translation: AAP35603.1 |
CCDSi | CCDS33936.1 [Q7L5Y9-1] CCDS33937.1 [Q7L5Y9-3] CCDS77887.1 [Q7L5Y9-4] |
RefSeqi | NP_001017405.1, NM_001017405.2 [Q7L5Y9-1] NP_001284360.1, NM_001297431.1 NP_005873.2, NM_005882.4 [Q7L5Y9-3] |
3D structure databases
SMRi | Q7L5Y9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 115584, 90 interactors |
CORUMi | Q7L5Y9 |
IntActi | Q7L5Y9, 39 interactors |
STRINGi | 9606.ENSP00000302830 |
Chemistry databases
DrugBanki | DB05389, Tetrachlorodecaoxide |
PTM databases
iPTMneti | Q7L5Y9 |
PhosphoSitePlusi | Q7L5Y9 |
Genetic variation databases
BioMutai | MAEA |
DMDMi | 74754297 |
Proteomic databases
EPDi | Q7L5Y9 |
jPOSTi | Q7L5Y9 |
MassIVEi | Q7L5Y9 |
MaxQBi | Q7L5Y9 |
PaxDbi | Q7L5Y9 |
PeptideAtlasi | Q7L5Y9 |
PRIDEi | Q7L5Y9 |
ProteomicsDBi | 68819 [Q7L5Y9-1] 68820 [Q7L5Y9-2] 68821 [Q7L5Y9-3] 68822 [Q7L5Y9-4] 68823 [Q7L5Y9-5] |
Protocols and materials databases
Antibodypediai | 8374, 270 antibodies |
DNASUi | 10296 |
Genome annotation databases
Ensembli | ENST00000264750; ENSP00000264750; ENSG00000090316 [Q7L5Y9-3] ENST00000303400; ENSP00000302830; ENSG00000090316 [Q7L5Y9-1] |
GeneIDi | 10296 |
KEGGi | hsa:10296 |
UCSCi | uc003gda.4, human [Q7L5Y9-1] |
Organism-specific databases
CTDi | 10296 |
DisGeNETi | 10296 |
GeneCardsi | MAEA |
HGNCi | HGNC:13731, MAEA |
HPAi | ENSG00000090316, Low tissue specificity |
MIMi | 606801, gene |
neXtProti | NX_Q7L5Y9 |
OpenTargetsi | ENSG00000090316 |
PharmGKBi | PA30533 |
VEuPathDBi | HostDB:ENSG00000090316.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0396, Eukaryota |
GeneTreei | ENSGT00940000153203 |
HOGENOMi | CLU_027445_0_1_1 |
InParanoidi | Q7L5Y9 |
OrthoDBi | 1087488at2759 |
PhylomeDBi | Q7L5Y9 |
TreeFami | TF314273 |
Enzyme and pathway databases
PathwayCommonsi | Q7L5Y9 |
Miscellaneous databases
BioGRID-ORCSi | 10296, 205 hits in 1008 CRISPR screens |
ChiTaRSi | MAEA, human |
GenomeRNAii | 10296 |
Pharosi | Q7L5Y9, Tbio |
PROi | PR:Q7L5Y9 |
RNActi | Q7L5Y9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000090316, Expressed in blood and 238 other tissues |
ExpressionAtlasi | Q7L5Y9, baseline and differential |
Genevisiblei | Q7L5Y9, HS |
Family and domain databases
InterProi | View protein in InterPro IPR013144, CRA_dom IPR024964, CTLH/CRA IPR006595, CTLH_C IPR027714, Fyv10/EMP IPR006594, LisH IPR044063, ZF_RING_GID |
PANTHERi | PTHR12170:SF2, PTHR12170:SF2, 1 hit |
Pfami | View protein in Pfam PF10607, CLTH, 1 hit |
SMARTi | View protein in SMART SM00757, CRA, 1 hit SM00668, CTLH, 1 hit SM00667, LisH, 1 hit |
PROSITEi | View protein in PROSITE PS50897, CTLH, 1 hit PS50896, LISH, 1 hit PS51867, ZF_RING_GID, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAEA_HUMAN | |
Accessioni | Q7L5Y9Primary (citable) accession number: Q7L5Y9 Secondary accession number(s): O95285 Q9NW84 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | July 5, 2005 | |
Last modified: | April 7, 2021 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot