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Protein

Golgi to ER traffic protein 4 homolog

Gene

GET4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20676083, PubMed:28104892, PubMed:25535373). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome (PubMed:28104892). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303).5 Publications

GO - Molecular functioni

  • chaperone binding Source: BHF-UCL

GO - Biological processi

  • cytoplasmic sequestering of protein Source: ParkinsonsUK-UCL
  • maintenance of unfolded protein involved in ERAD pathway Source: ParkinsonsUK-UCL
  • protein insertion into ER membrane Source: GO_Central
  • tail-anchored membrane protein insertion into ER membrane Source: UniProtKB

Keywordsi

Biological processTransport

Protein family/group databases

MoonDBiQ7L5D6 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi to ER traffic protein 4 homologCurated
Alternative name(s):
Conserved edge-expressed protein
Transmembrane domain recognition complex 35 kDa subunit
Short name:
TRC35
Gene namesi
Name:GET4Imported
Synonyms:C7orf20, CEE, TRC35
ORF Names:CGI-20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000239857.6
HGNCiHGNC:21690 GET4
MIMi612056 gene
neXtProtiNX_Q7L5D6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi84D → K: Reduces tail-anchored protein delivery. 1 Publication1
Mutagenesisi182Y → A: No effect on interaction with BAG6. 1 Publication1
Mutagenesisi188F → A: No effect on interaction with BAG6. 1 Publication1
Mutagenesisi195F → A: No effect on interaction with BAG6. 1 Publication1
Mutagenesisi241W → A: No effect on interaction with BAG6. 1 Publication1
Mutagenesisi242F → A: No effect on interaction with BAG6. 1 Publication1
Mutagenesisi257V → A: No effect on interaction with BAG6. 1 Publication1
Mutagenesisi258L → A: No effect on interaction with BAG6. 1 Publication1
Mutagenesisi259C → A: No effect on interaction with BAG6. 1 Publication1
Mutagenesisi262Y → A: Inhibits interaction with BAG6. 1 Publication1
Mutagenesisi266L → A: No effect on interaction with BAG6. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000239857
PharmGKBiPA165618100

Polymorphism and mutation databases

BioMutaiGET4
DMDMi74738593

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002281042 – 327Golgi to ER traffic protein 4 homologAdd BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei12PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated by RNF12, leading to proteasomal degradation. When unassembled from BAG6; ubiquitinylation is modulated by BAG6 quality control role and effectuated by RNF126.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ7L5D6
MaxQBiQ7L5D6
PaxDbiQ7L5D6
PeptideAtlasiQ7L5D6
PRIDEiQ7L5D6
ProteomicsDBi68807
68808 [Q7L5D6-2]

PTM databases

iPTMnetiQ7L5D6
PhosphoSitePlusiQ7L5D6

Expressioni

Gene expression databases

BgeeiENSG00000239857 Expressed in 88 organ(s), highest expression level in right adrenal gland
CleanExiHS_C7orf20
ExpressionAtlasiQ7L5D6 baseline and differential
GenevisibleiQ7L5D6 HS

Organism-specific databases

HPAiHPA019765

Interactioni

Subunit structurei

Component of the BAG6/BAT3 complex, at least composed of BAG6, UBL4A and GET4/TRC35 (PubMed:20676083, PubMed:25535373). Interacts with BAG6; the interaction is direct and localizes BAG6 to the cytosol (PubMed:21636303, PubMed:29042515, PubMed:25535373).4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119636, 64 interactors
ComplexPortaliCPX-132 BAT3 complex
IntActiQ7L5D6, 46 interactors
MINTiQ7L5D6
STRINGi9606.ENSP00000265857

Structurei

Secondary structure

1327
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ7L5D6
SMRiQ7L5D6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 271Interacts with BAG62 PublicationsAdd BLAST77

Sequence similaritiesi

Belongs to the GET4 family.Curated

Phylogenomic databases

eggNOGiKOG3024 Eukaryota
ENOG410XRWE LUCA
GeneTreeiENSGT00390000015750
HOGENOMiHOG000264232
HOVERGENiHBG059877
InParanoidiQ7L5D6
OMAiRYMSQGK
OrthoDBiEOG091G0DTD
PhylomeDBiQ7L5D6
TreeFamiTF315163

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR011990 TPR-like_helical_dom_sf
IPR007317 UPF0363
PANTHERiPTHR12875 PTHR12875, 1 hit
PfamiView protein in Pfam
PF04190 DUF410, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7L5D6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAAAAMAEQ ESARNGGRNR GGVQRVEGKL RASVEKGDYY EAHQMYRTLF
60 70 80 90 100
FRYMSQSKHT EARELMYSGA LLFFSHGQQN SAADLSMLVL ESLEKAEVEV
110 120 130 140 150
ADELLENLAK VFSLMDPNSP ERVTFVSRAL KWSSGGSGKL GHPRLHQLLA
160 170 180 190 200
LTLWKEQNYC ESRYHFLHSA DGEGCANMLV EYSTSRGFRS EVDMFVAQAV
210 220 230 240 250
LQFLCLKNKS SASVVFTTYT QKHPSIEDGP PFVEPLLNFI WFLLLAVDGG
260 270 280 290 300
KLTVFTVLCE QYQPSLRRDP MYNEYLDRIG QLFFGVPPKQ TSSYGGLLGN
310 320
LLTSLMGSSE QEDGEESPSD GSPIELD
Note: No experimental confirmation available.
Length:327
Mass (Da):36,504
Last modified:July 5, 2004 - v1
Checksum:i4D6C4233181B0512
GO
Isoform 2 (identifier: Q7L5D6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Show »
Length:274
Mass (Da):30,523
Checksum:iCDCA760B2B2E1473
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JPA8C9JPA8_HUMAN
Golgi to ER traffic protein 4 homol...
GET4
148Annotation score:
C9JHN8C9JHN8_HUMAN
Golgi to ER traffic protein 4 homol...
GET4
167Annotation score:
C9JY46C9JY46_HUMAN
Golgi to ER traffic protein 4 homol...
GET4
158Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9 – 22EQESA…RNRGG → DRRAPATALQPRA in AAD27729 (PubMed:10810093).CuratedAdd BLAST14
Sequence conflicti177N → D in AAD27729 (PubMed:10810093).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0176521 – 53Missing in isoform 2. 2 PublicationsAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024305 mRNA Translation: BAG51289.1
AK097899 mRNA Translation: BAG53546.1
AK125863 mRNA Translation: BAG54258.1
AL133014 mRNA Translation: CAB61355.1
CH236965 Genomic DNA Translation: EAL23708.1
BC003550 mRNA Translation: AAH03550.2
AF132954 mRNA Translation: AAD27729.1
CCDSiCCDS5317.1 [Q7L5D6-1]
PIRiT42648
RefSeqiNP_057033.2, NM_015949.2 [Q7L5D6-1]
UniGeneiHs.107387
Hs.743756

Genome annotation databases

EnsembliENST00000265857; ENSP00000265857; ENSG00000239857 [Q7L5D6-1]
ENST00000407192; ENSP00000385646; ENSG00000239857 [Q7L5D6-2]
GeneIDi51608
KEGGihsa:51608
UCSCiuc003sjl.2 human [Q7L5D6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024305 mRNA Translation: BAG51289.1
AK097899 mRNA Translation: BAG53546.1
AK125863 mRNA Translation: BAG54258.1
AL133014 mRNA Translation: CAB61355.1
CH236965 Genomic DNA Translation: EAL23708.1
BC003550 mRNA Translation: AAH03550.2
AF132954 mRNA Translation: AAD27729.1
CCDSiCCDS5317.1 [Q7L5D6-1]
PIRiT42648
RefSeqiNP_057033.2, NM_015949.2 [Q7L5D6-1]
UniGeneiHs.107387
Hs.743756

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6AU8X-ray1.80A23-305[»]
ProteinModelPortaliQ7L5D6
SMRiQ7L5D6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119636, 64 interactors
ComplexPortaliCPX-132 BAT3 complex
IntActiQ7L5D6, 46 interactors
MINTiQ7L5D6
STRINGi9606.ENSP00000265857

Protein family/group databases

MoonDBiQ7L5D6 Predicted

PTM databases

iPTMnetiQ7L5D6
PhosphoSitePlusiQ7L5D6

Polymorphism and mutation databases

BioMutaiGET4
DMDMi74738593

Proteomic databases

EPDiQ7L5D6
MaxQBiQ7L5D6
PaxDbiQ7L5D6
PeptideAtlasiQ7L5D6
PRIDEiQ7L5D6
ProteomicsDBi68807
68808 [Q7L5D6-2]

Protocols and materials databases

DNASUi51608
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265857; ENSP00000265857; ENSG00000239857 [Q7L5D6-1]
ENST00000407192; ENSP00000385646; ENSG00000239857 [Q7L5D6-2]
GeneIDi51608
KEGGihsa:51608
UCSCiuc003sjl.2 human [Q7L5D6-1]

Organism-specific databases

CTDi51608
EuPathDBiHostDB:ENSG00000239857.6
GeneCardsiGET4
H-InvDBiHIX0033841
HIX0201122
HGNCiHGNC:21690 GET4
HPAiHPA019765
MIMi612056 gene
neXtProtiNX_Q7L5D6
OpenTargetsiENSG00000239857
PharmGKBiPA165618100
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3024 Eukaryota
ENOG410XRWE LUCA
GeneTreeiENSGT00390000015750
HOGENOMiHOG000264232
HOVERGENiHBG059877
InParanoidiQ7L5D6
OMAiRYMSQGK
OrthoDBiEOG091G0DTD
PhylomeDBiQ7L5D6
TreeFamiTF315163

Miscellaneous databases

GeneWikiiC7orf20
GenomeRNAii51608
PROiPR:Q7L5D6
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000239857 Expressed in 88 organ(s), highest expression level in right adrenal gland
CleanExiHS_C7orf20
ExpressionAtlasiQ7L5D6 baseline and differential
GenevisibleiQ7L5D6 HS

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR011990 TPR-like_helical_dom_sf
IPR007317 UPF0363
PANTHERiPTHR12875 PTHR12875, 1 hit
PfamiView protein in Pfam
PF04190 DUF410, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGET4_HUMAN
AccessioniPrimary (citable) accession number: Q7L5D6
Secondary accession number(s): A4D2Q1
, B3KNC7, Q9UFC9, Q9Y309
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
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