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Protein

Docking protein 3

Gene

DOK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function (By similarity).By similarity

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation
SignaLinkiQ7L591

Names & Taxonomyi

Protein namesi
Recommended name:
Docking protein 3
Alternative name(s):
Downstream of tyrosine kinase 3
Gene namesi
Name:DOK3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000146094.13
HGNCiHGNC:24583 DOK3
MIMi611435 gene
neXtProtiNX_Q7L591

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi79930
OpenTargetsiENSG00000146094
PharmGKBiPA128394725

Polymorphism and mutation databases

BioMutaiDOK3
DMDMi84029599

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872721 – 496Docking protein 3Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei194PhosphoserineCombined sources1
Modified residuei330PhosphoserineCombined sources1
Modified residuei364PhosphoserineBy similarity1
Modified residuei381PhosphotyrosineBy similarity1
Modified residuei425PhosphoserineBy similarity1

Post-translational modificationi

Constitutively tyrosine-phosphorylated.By similarity
On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7L591
MaxQBiQ7L591
PaxDbiQ7L591
PeptideAtlasiQ7L591
PRIDEiQ7L591
ProteomicsDBi68797
68798 [Q7L591-2]
68799 [Q7L591-3]
68800 [Q7L591-4]

PTM databases

iPTMnetiQ7L591
PhosphoSitePlusiQ7L591

Expressioni

Tissue specificityi

Expressed in spleen.1 Publication

Gene expression databases

BgeeiENSG00000146094 Expressed in 138 organ(s), highest expression level in blood
CleanExiHS_DOK3
ExpressionAtlasiQ7L591 baseline and differential
GenevisibleiQ7L591 HS

Organism-specific databases

HPAiHPA077312

Interactioni

Subunit structurei

On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL1 through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP (By similarity).By similarity

Binary interactionsi

Protein-protein interaction databases

BioGridi123005, 27 interactors
IntActiQ7L591, 32 interactors
MINTiQ7L591
STRINGi9606.ENSP00000349727

Structurei

3D structure databases

ProteinModelPortaliQ7L591
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 179PHAdd BLAST117
Domaini213 – 317IRS-type PTBPROSITE-ProRule annotationAdd BLAST105

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi320 – 373Pro-richAdd BLAST54
Compositional biasi391 – 496Pro-richAdd BLAST106

Domaini

PTB domain mediates receptor interaction.By similarity

Sequence similaritiesi

Belongs to the DOK family. Type A subfamily.Curated

Phylogenomic databases

eggNOGiKOG4047 Eukaryota
ENOG410XS2S LUCA
GeneTreeiENSGT00730000110348
HOGENOMiHOG000112245
HOVERGENiHBG051391
InParanoidiQ7L591
OMAiKGPAPCD
OrthoDBiEOG091G075T
PhylomeDBiQ7L591
TreeFamiTF324994

Family and domain databases

CDDicd01203 PTB_DOK1_DOK2_DOK3, 1 hit
Gene3Di2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR037751 Dok1/2/3_PTB
IPR002404 IRS_PTB
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PfamiView protein in Pfam
PF02174 IRS, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00310 PTBI, 1 hit
PROSITEiView protein in PROSITE
PS51064 IRS_PTB, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7L591-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTRGARLRSD ARAQLNQLSL DGGTGSGQKG KCEEFPSSLS SVSPGLEAAA
60 70 80 90 100
LLLAVTMDPL ETPIKDGILY QQHVKFGKKC WRKVWALLYA GGPSGVARLE
110 120 130 140 150
SWEVRDGGLG AAGDRSAGPG RRGERRVIRL ADCVSVLPAD GESCPRDTGA
160 170 180 190 200
FLLTTTERSH LLAAQHRQAW MGPICQLAFP GTGEASSGST DAQSPKRGLV
210 220 230 240 250
PMEENSIYSS WQEVGEFPVV VQRTEAATRC QLKGPALLVL GPDAIQLREA
260 270 280 290 300
KGTQALYSWP YHFLRKFGSD KGVFSFEAGR RCHSGEGLFA FSTPCAPDLC
310 320 330 340 350
RAVAGAIARQ RERLPELTRP QPCPLPRATS LPSLDTPGEL REMPPGPEPP
360 370 380 390 400
TSRKMHLAEP GPQSLPLLLG PEPNDLASGL YASVCKRASG PPGNEHLYEN
410 420 430 440 450
LCVLEASPTL HGGEPEPHEG PGSRSPTTSP IYHNGQDLSW PGPANDSTLE
460 470 480 490
AQYRRLLELD QVEGTGRPDP QAGFKAKLVT LLSRERRKGP APCDRP
Note: No experimental confirmation available.
Length:496
Mass (Da):53,288
Last modified:May 10, 2005 - v2
Checksum:i7E186B998F9F1E24
GO
Isoform 2 (identifier: Q7L591-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-170: Missing.
     272-386: GVFSFEAGRR...ASGLYASVCK → ILLGTPGVSL...TSAAHTLGTS
     387-496: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:216
Mass (Da):23,255
Checksum:i3276F905C619BC3D
GO
Isoform 3 (identifier: Q7L591-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.
     272-386: GVFSFEAGRR...ASGLYASVCK → ILLGTPGVSL...TSAAHTLGTS
     387-496: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:330
Mass (Da):35,735
Checksum:iE4D842CAE0D6D8C3
GO
Isoform 4 (identifier: Q7L591-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.
     79-180: Missing.
     272-386: GVFSFEAGRR...ASGLYASVCK → ILLGTPGVSL...TSAAHTLGTS
     387-496: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:228
Mass (Da):24,737
Checksum:i09009F00CD8DF2F9
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RAM3D6RAM3_HUMAN
Docking protein 3
DOK3
440Annotation score:
D6RC22D6RC22_HUMAN
Docking protein 3
DOK3
213Annotation score:
D6R977D6R977_HUMAN
Docking protein 3
DOK3
156Annotation score:
D6R951D6R951_HUMAN
Docking protein 3
DOK3
144Annotation score:
D6RAZ9D6RAZ9_HUMAN
Docking protein 3
DOK3
43Annotation score:
D6RF29D6RF29_HUMAN
Docking protein 3
DOK3
13Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti267F → L in BAB15399 (PubMed:14702039).Curated1
Isoform 2 (identifier: Q7L591-2)
Sequence conflicti188R → C in AAH04867 (PubMed:15372022).Curated1
Isoform 4 (identifier: Q7L591-4)
Sequence conflicti200R → C in BAC04986 (PubMed:14702039).Curated1
Isoform 3 (identifier: Q7L591-3)
Sequence conflicti302R → C in AAH04564 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05752512R → P. Corresponds to variant dbSNP:rs3749728Ensembl.1
Natural variantiVAR_06200222G → R. Corresponds to variant dbSNP:rs41275297Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0137131 – 170Missing in isoform 2. 1 PublicationAdd BLAST170
Alternative sequenceiVSP_0137121 – 56Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST56
Alternative sequenceiVSP_01371479 – 180Missing in isoform 4. 1 PublicationAdd BLAST102
Alternative sequenceiVSP_013715272 – 386GVFSF…ASVCK → ILLGTPGVSLLICKGERTDD VSGIILDESLLRAYSVPGAG GHSRVQDSLGPVLREPTFQG ERSFLKTSMLRSLLCSCSWR HPRSQPRTQASCLQGSDCPA PHRNSTSAAHTLGTS in isoform 2, isoform 3 and isoform 4. 2 PublicationsAdd BLAST115
Alternative sequenceiVSP_013716387 – 496Missing in isoform 2, isoform 3 and isoform 4. 2 PublicationsAdd BLAST110

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026223 mRNA Translation: BAB15399.1
AK097258 mRNA Translation: BAC04986.1
AC145098 Genomic DNA No translation available.
BC004564 mRNA Translation: AAH04564.2
BC004867 mRNA Translation: AAH04867.1
CCDSiCCDS4426.1 [Q7L591-1]
CCDS47349.1 [Q7L591-4]
CCDS47350.1 [Q7L591-3]
RefSeqiNP_001138347.1, NM_001144875.1 [Q7L591-3]
NP_001138348.1, NM_001144876.1 [Q7L591-4]
NP_001295164.1, NM_001308235.1
NP_001295165.1, NM_001308236.1
NP_079148.2, NM_024872.2 [Q7L591-1]
XP_005266044.1, XM_005265987.1 [Q7L591-3]
UniGeneiHs.720849

Genome annotation databases

EnsembliENST00000312943; ENSP00000325174; ENSG00000146094 [Q7L591-3]
ENST00000357198; ENSP00000349727; ENSG00000146094 [Q7L591-1]
ENST00000377112; ENSP00000366316; ENSG00000146094 [Q7L591-4]
GeneIDi79930
KEGGihsa:79930
UCSCiuc003mhi.5 human [Q7L591-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026223 mRNA Translation: BAB15399.1
AK097258 mRNA Translation: BAC04986.1
AC145098 Genomic DNA No translation available.
BC004564 mRNA Translation: AAH04564.2
BC004867 mRNA Translation: AAH04867.1
CCDSiCCDS4426.1 [Q7L591-1]
CCDS47349.1 [Q7L591-4]
CCDS47350.1 [Q7L591-3]
RefSeqiNP_001138347.1, NM_001144875.1 [Q7L591-3]
NP_001138348.1, NM_001144876.1 [Q7L591-4]
NP_001295164.1, NM_001308235.1
NP_001295165.1, NM_001308236.1
NP_079148.2, NM_024872.2 [Q7L591-1]
XP_005266044.1, XM_005265987.1 [Q7L591-3]
UniGeneiHs.720849

3D structure databases

ProteinModelPortaliQ7L591
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123005, 27 interactors
IntActiQ7L591, 32 interactors
MINTiQ7L591
STRINGi9606.ENSP00000349727

PTM databases

iPTMnetiQ7L591
PhosphoSitePlusiQ7L591

Polymorphism and mutation databases

BioMutaiDOK3
DMDMi84029599

Proteomic databases

EPDiQ7L591
MaxQBiQ7L591
PaxDbiQ7L591
PeptideAtlasiQ7L591
PRIDEiQ7L591
ProteomicsDBi68797
68798 [Q7L591-2]
68799 [Q7L591-3]
68800 [Q7L591-4]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312943; ENSP00000325174; ENSG00000146094 [Q7L591-3]
ENST00000357198; ENSP00000349727; ENSG00000146094 [Q7L591-1]
ENST00000377112; ENSP00000366316; ENSG00000146094 [Q7L591-4]
GeneIDi79930
KEGGihsa:79930
UCSCiuc003mhi.5 human [Q7L591-1]

Organism-specific databases

CTDi79930
DisGeNETi79930
EuPathDBiHostDB:ENSG00000146094.13
GeneCardsiDOK3
H-InvDBiHIX0200730
HGNCiHGNC:24583 DOK3
HPAiHPA077312
MIMi611435 gene
neXtProtiNX_Q7L591
OpenTargetsiENSG00000146094
PharmGKBiPA128394725
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4047 Eukaryota
ENOG410XS2S LUCA
GeneTreeiENSGT00730000110348
HOGENOMiHOG000112245
HOVERGENiHBG051391
InParanoidiQ7L591
OMAiKGPAPCD
OrthoDBiEOG091G075T
PhylomeDBiQ7L591
TreeFamiTF324994

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation
SignaLinkiQ7L591

Miscellaneous databases

ChiTaRSiDOK3 human
GeneWikiiDOK3
GenomeRNAii79930
PROiPR:Q7L591
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000146094 Expressed in 138 organ(s), highest expression level in blood
CleanExiHS_DOK3
ExpressionAtlasiQ7L591 baseline and differential
GenevisibleiQ7L591 HS

Family and domain databases

CDDicd01203 PTB_DOK1_DOK2_DOK3, 1 hit
Gene3Di2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR037751 Dok1/2/3_PTB
IPR002404 IRS_PTB
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PfamiView protein in Pfam
PF02174 IRS, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00310 PTBI, 1 hit
PROSITEiView protein in PROSITE
PS51064 IRS_PTB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDOK3_HUMAN
AccessioniPrimary (citable) accession number: Q7L591
Secondary accession number(s): E9PAT0
, H7BXS0, Q8N864, Q9BQB3, Q9H666
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: September 12, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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