UniProtKB - Q7L590 (MCM10_HUMAN)
Protein
Protein MCM10 homolog
Gene
MCM10
Organism
Homo sapiens (Human)
Status
Functioni
Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38 (PubMed:24726359).5 Publications
GO - Molecular functioni
- DNA replication origin binding Source: GO_Central
- double-stranded DNA binding Source: GO_Central
- enzyme binding Source: Ensembl
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- single-stranded DNA binding Source: GO_Central
GO - Biological processi
- cell population proliferation Source: Ensembl
- cellular response to DNA damage stimulus Source: UniProtKB
- DNA replication Source: Reactome
- DNA replication initiation Source: GO_Central
- G1/S transition of mitotic cell cycle Source: Reactome
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA replication |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q7L590 |
Reactomei | R-HSA-176187, Activation of ATR in response to replication stress R-HSA-68962, Activation of the pre-replicative complex |
SIGNORi | Q7L590 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein MCM10 homologShort name: HsMCM10 |
Gene namesi | Name:MCM10 ORF Names:PRO2249 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18043, MCM10 |
MIMi | 609357, gene |
neXtProti | NX_Q7L590 |
VEuPathDBi | HostDB:ENSG00000065328.16 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Note: Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli.
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
- replication fork protection complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 55388 |
OpenTargetsi | ENSG00000065328 |
PharmGKBi | PA30689 |
Miscellaneous databases
Pharosi | Q7L590, Tbio |
Genetic variation databases
BioMutai | MCM10 |
DMDMi | 126215746 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000278320 | 1 – 875 | Protein MCM10 homologAdd BLAST | 875 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 85 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 93 | PhosphoserineCombined sources | 1 | |
Cross-linki | 493 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 627 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 644 | PhosphoserineBy similarity | 1 | |
Cross-linki | 762 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 763 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | CPTAC-1210 CPTAC-1211 |
EPDi | Q7L590 |
jPOSTi | Q7L590 |
MassIVEi | Q7L590 |
MaxQBi | Q7L590 |
PaxDbi | Q7L590 |
PeptideAtlasi | Q7L590 |
PRIDEi | Q7L590 |
ProteomicsDBi | 68795 [Q7L590-1] 68796 [Q7L590-2] |
PTM databases
iPTMneti | Q7L590 |
MetOSitei | Q7L590 |
PhosphoSitePlusi | Q7L590 |
Expressioni
Developmental stagei
Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G1 phase, and starts to accumulate at the onset of S phase.2 Publications
Gene expression databases
Bgeei | ENSG00000065328, Expressed in amniotic fluid and 131 other tissues |
ExpressionAtlasi | Q7L590, baseline and differential |
Genevisiblei | Q7L590, HS |
Organism-specific databases
HPAi | ENSG00000065328, Tissue enhanced (bone marrow, lymphoid tissue) |
Interactioni
Subunit structurei
Self-associates (By similarity).
Interacts with ORC2. May interact with MCM2 and MCM6.
Interacts with the DNA polymerase alpha subunit POLA1.
Interacts with RECQL4; this interaction regulates RECQL4 unwinding activity.
Interacts with WDHD1.
By similarity3 PublicationsBinary interactionsi
Hide detailsQ7L590
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-374912,EBI-374912 |
MCM6 [Q14566] | 2 | EBI-374912,EBI-374900 |
ORC2 [Q13416] | 5 | EBI-374912,EBI-374957 |
Isoform 2 [Q7L590-2]
GO - Molecular functioni
- enzyme binding Source: Ensembl
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 120654, 79 interactors |
IntActi | Q7L590, 52 interactors |
MINTi | Q7L590 |
STRINGi | 9606.ENSP00000418268 |
Miscellaneous databases
RNActi | Q7L590, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 156 | N-terminal domainBy similarityAdd BLAST | 156 | |
Regioni | 238 – 389 | OB-fold domainBy similarityAdd BLAST | 152 | |
Regioni | 390 – 415 | Zinc finger-like 1Add BLAST | 26 | |
Regioni | 783 – 802 | Zinc finger-like 2By similarityAdd BLAST | 20 | |
Regioni | 816 – 836 | Zinc finger-like 3By similarityAdd BLAST | 21 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 104 – 142 | Sequence analysisAdd BLAST | 39 |
Domaini
Each zinc finger-like domain binds a zinc ion and is involved in both ssDNA and dsDNA binding, as is the OB-fold domain.By similarity
The N-terminal domain mediates homodimerization.By similarity
Sequence similaritiesi
Belongs to the MCM10 family.Curated
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG3056, Eukaryota |
GeneTreei | ENSGT00390000007134 |
HOGENOMi | CLU_014680_0_0_1 |
InParanoidi | Q7L590 |
OMAi | HIERGDL |
OrthoDBi | 758449at2759 |
PhylomeDBi | Q7L590 |
TreeFami | TF313330 |
Family and domain databases
InterProi | View protein in InterPro IPR040184, Mcm10 IPR015411, Rep_factor_Mcm10_C IPR015408, Znf_Mcm10/DnaG |
PANTHERi | PTHR13454, PTHR13454, 1 hit |
Pfami | View protein in Pfam PF09332, Mcm10, 1 hit PF09329, zf-primase, 1 hit |
SMARTi | View protein in SMART SM01280, Mcm10, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q7L590-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDEEEDNLSL LTALLEENES ALDCNSEENN FLTRENGEPD AFDELFDADG
60 70 80 90 100
DGESYTEEAD DGETGETRDE KENLATLFGD MEDLTDEEEV PASQSTENRV
110 120 130 140 150
LPAPAPRREK TNEELQEELR NLQEQMKALQ EQLKVTTIKQ TASPARLQKS
160 170 180 190 200
PVEKSPRPPL KERRVQRIQE STCFSAELDV PALPRTKRVA RTPKASPPDP
210 220 230 240 250
KSSSSRMTSA PSQPLQTISR NKPSGITRGQ IVGTPGSSGE TTQPICVEAF
260 270 280 290 300
SGLRLRRPRV SSTEMNKKMT GRKLIRLSQI KEKMAREKLE EIDWVTFGVI
310 320 330 340 350
LKKVTPQSVN SGKTFSIWKL NDLRDLTQCV SLFLFGEVHK ALWKTEQGTV
360 370 380 390 400
VGILNANPMK PKDGSEEVCL SIDHPQKVLI MGEALDLGTC KAKKKNGEPC
410 420 430 440 450
TQTVNLRDCE YCQYHVQAQY KKLSAKRADL QSTFSGGRIP KKFARRGTSL
460 470 480 490 500
KERLCQDGFY YGGVSSASYA ASIAAAVAPK KKIQTTLSNL VVKGTNLIIQ
510 520 530 540 550
ETRQKLGIPQ KSLSCSEEFK ELMDLPTCGA RNLKQHLAKA TASGIMGSPK
560 570 580 590 600
PAIKSISASA LLKQQKQRML EMRRRKSEEI QKRFLQSSSE VESPAVPSSS
610 620 630 640 650
RQPPAQPPRT GSEFPRLEGA PATMTPKLGR GVLEGDDVLF YDESPPPRPK
660 670 680 690 700
LSALAEAKKL AAITKLRAKG QVLTKTNPNS IKKKQKDPQD ILEVKERVEK
710 720 730 740 750
NTMFSSQAED ELEPARKKRR EQLAYLESEE FQKILKAKSK HTGILKEAEA
760 770 780 790 800
EMQERYFEPL VKKEQMEEKM RNIREVKCRV VTCKTCAYTH FKLLETCVSE
810 820 830 840 850
QHEYHWHDGV KRFFKCPCGN RSISLDRLPN KHCSNCGLYK WERDGMLKEK
860 870
TGPKIGGETL LPRGEEHAKF LNSLK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5T670 | Q5T670_HUMAN | Protein MCM10 homolog | MCM10 hCG_40780 | 855 | Annotation score: | ||
C9J600 | C9J600_HUMAN | Protein MCM10 homolog | MCM10 | 69 | Annotation score: |
Sequence cautioni
The sequence AAF69623 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 303 | K → R in BAB70988 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_030771 | 134 | K → R1 PublicationCorresponds to variant dbSNP:rs17152897Ensembl. | 1 | |
Natural variantiVAR_053836 | 195 | A → P2 PublicationsCorresponds to variant dbSNP:rs34630110Ensembl. | 1 | |
Natural variantiVAR_053837 | 418 | A → V. Corresponds to variant dbSNP:rs35114749Ensembl. | 1 | |
Natural variantiVAR_030772 | 541 | T → S2 PublicationsCorresponds to variant dbSNP:rs7905784Ensembl. | 1 | |
Natural variantiVAR_030773 | 669 | K → R1 PublicationCorresponds to variant dbSNP:rs2274110Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029951 | 152 | Missing in isoform 2. 3 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB042719 mRNA Translation: BAB18723.1 AL136840 mRNA Translation: CAB66774.2 AK055695 mRNA Translation: BAB70988.1 AK292701 mRNA Translation: BAF85390.1 AL355355 Genomic DNA No translation available. AL138764 Genomic DNA No translation available. CH471072 Genomic DNA Translation: EAW86297.1 BC004876 mRNA Translation: AAH04876.2 BC101727 mRNA Translation: AAI01728.1 BC143490 mRNA Translation: AAI43491.1 AF119869 mRNA Translation: AAF69623.1 Different initiation. |
CCDSi | CCDS7095.1 [Q7L590-2] CCDS7096.1 [Q7L590-1] |
RefSeqi | NP_060988.3, NM_018518.4 [Q7L590-2] NP_877428.1, NM_182751.2 [Q7L590-1] XP_011517840.1, XM_011519538.2 [Q7L590-1] |
Genome annotation databases
Ensembli | ENST00000378714; ENSP00000367986; ENSG00000065328 [Q7L590-2] ENST00000484800; ENSP00000418268; ENSG00000065328 [Q7L590-1] |
GeneIDi | 55388 |
KEGGi | hsa:55388 |
UCSCi | uc001ima.4, human [Q7L590-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB042719 mRNA Translation: BAB18723.1 AL136840 mRNA Translation: CAB66774.2 AK055695 mRNA Translation: BAB70988.1 AK292701 mRNA Translation: BAF85390.1 AL355355 Genomic DNA No translation available. AL138764 Genomic DNA No translation available. CH471072 Genomic DNA Translation: EAW86297.1 BC004876 mRNA Translation: AAH04876.2 BC101727 mRNA Translation: AAI01728.1 BC143490 mRNA Translation: AAI43491.1 AF119869 mRNA Translation: AAF69623.1 Different initiation. |
CCDSi | CCDS7095.1 [Q7L590-2] CCDS7096.1 [Q7L590-1] |
RefSeqi | NP_060988.3, NM_018518.4 [Q7L590-2] NP_877428.1, NM_182751.2 [Q7L590-1] XP_011517840.1, XM_011519538.2 [Q7L590-1] |
3D structure databases
SMRi | Q7L590 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 120654, 79 interactors |
IntActi | Q7L590, 52 interactors |
MINTi | Q7L590 |
STRINGi | 9606.ENSP00000418268 |
PTM databases
iPTMneti | Q7L590 |
MetOSitei | Q7L590 |
PhosphoSitePlusi | Q7L590 |
Genetic variation databases
BioMutai | MCM10 |
DMDMi | 126215746 |
Proteomic databases
CPTACi | CPTAC-1210 CPTAC-1211 |
EPDi | Q7L590 |
jPOSTi | Q7L590 |
MassIVEi | Q7L590 |
MaxQBi | Q7L590 |
PaxDbi | Q7L590 |
PeptideAtlasi | Q7L590 |
PRIDEi | Q7L590 |
ProteomicsDBi | 68795 [Q7L590-1] 68796 [Q7L590-2] |
Protocols and materials databases
Antibodypediai | 11355, 141 antibodies |
Genome annotation databases
Ensembli | ENST00000378714; ENSP00000367986; ENSG00000065328 [Q7L590-2] ENST00000484800; ENSP00000418268; ENSG00000065328 [Q7L590-1] |
GeneIDi | 55388 |
KEGGi | hsa:55388 |
UCSCi | uc001ima.4, human [Q7L590-1] |
Organism-specific databases
CTDi | 55388 |
DisGeNETi | 55388 |
GeneCardsi | MCM10 |
HGNCi | HGNC:18043, MCM10 |
HPAi | ENSG00000065328, Tissue enhanced (bone marrow, lymphoid tissue) |
MIMi | 609357, gene |
neXtProti | NX_Q7L590 |
OpenTargetsi | ENSG00000065328 |
PharmGKBi | PA30689 |
VEuPathDBi | HostDB:ENSG00000065328.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3056, Eukaryota |
GeneTreei | ENSGT00390000007134 |
HOGENOMi | CLU_014680_0_0_1 |
InParanoidi | Q7L590 |
OMAi | HIERGDL |
OrthoDBi | 758449at2759 |
PhylomeDBi | Q7L590 |
TreeFami | TF313330 |
Enzyme and pathway databases
PathwayCommonsi | Q7L590 |
Reactomei | R-HSA-176187, Activation of ATR in response to replication stress R-HSA-68962, Activation of the pre-replicative complex |
SIGNORi | Q7L590 |
Miscellaneous databases
BioGRID-ORCSi | 55388, 330 hits in 882 CRISPR screens |
ChiTaRSi | MCM10, human |
GeneWikii | MCM10 |
GenomeRNAii | 55388 |
Pharosi | Q7L590, Tbio |
PROi | PR:Q7L590 |
RNActi | Q7L590, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000065328, Expressed in amniotic fluid and 131 other tissues |
ExpressionAtlasi | Q7L590, baseline and differential |
Genevisiblei | Q7L590, HS |
Family and domain databases
InterProi | View protein in InterPro IPR040184, Mcm10 IPR015411, Rep_factor_Mcm10_C IPR015408, Znf_Mcm10/DnaG |
PANTHERi | PTHR13454, PTHR13454, 1 hit |
Pfami | View protein in Pfam PF09332, Mcm10, 1 hit PF09329, zf-primase, 1 hit |
SMARTi | View protein in SMART SM01280, Mcm10, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM10_HUMAN | |
Accessioni | Q7L590Primary (citable) accession number: Q7L590 Secondary accession number(s): A8K9I6 Q9P177 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | February 20, 2007 | |
Last modified: | February 10, 2021 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families