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UniProtKB - Q7L590 (MCM10_HUMAN)

Protein

Protein MCM10 homolog

Gene

MCM10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38 (PubMed:24726359).5 Publications

GO - Molecular functioni

  • DNA replication origin binding Source: GO_Central
  • double-stranded DNA binding Source: GO_Central
  • enzyme binding Source: Ensembl
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • single-stranded DNA binding Source: GO_Central
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA replication
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-176187 Activation of ATR in response to replication stress
R-HSA-68962 Activation of the pre-replicative complex
SIGNORiQ7L590

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MCM10 homolog
Short name:
HsMCM10
Gene namesi
Name:MCM10
ORF Names:PRO2249
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000065328.16
HGNCiHGNC:18043 MCM10
MIMi609357 gene
neXtProtiNX_Q7L590

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55388
OpenTargetsiENSG00000065328
PharmGKBiPA30689

Polymorphism and mutation databases

BioMutaiMCM10
DMDMi126215746

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002783201 – 875Protein MCM10 homologAdd BLAST875

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei85PhosphothreonineCombined sources1
Modified residuei93PhosphoserineCombined sources1
Cross-linki493Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki627Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei644PhosphoserineBy similarity1
Cross-linki762Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki763Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ7L590
MaxQBiQ7L590
PaxDbiQ7L590
PeptideAtlasiQ7L590
PRIDEiQ7L590
ProteomicsDBi68795
68796 [Q7L590-2]

PTM databases

iPTMnetiQ7L590
PhosphoSitePlusiQ7L590

Expressioni

Developmental stagei

Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G1 phase, and starts to accumulate at the onset of S phase.2 Publications

Gene expression databases

BgeeiENSG00000065328 Expressed in 110 organ(s), highest expression level in amniotic fluid
CleanExiHS_MCM10
ExpressionAtlasiQ7L590 baseline and differential
GenevisibleiQ7L590 HS

Organism-specific databases

HPAiHPA045899
HPA071952

Interactioni

Subunit structurei

Self-associates (By similarity). Interacts with ORC2. May interact with MCM2 and MCM6. Interacts with the DNA polymerase alpha subunit POLA1. Interacts with RECQL4; this interaction regulates RECQL4 unwinding activity. Interacts with WDHD1.By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi120654, 67 interactors
IntActiQ7L590, 40 interactors
MINTiQ7L590
STRINGi9606.ENSP00000354945

Structurei

3D structure databases

ProteinModelPortaliQ7L590
SMRiQ7L590
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 156N-terminal domainBy similarityAdd BLAST156
Regioni238 – 389OB-fold domainBy similarityAdd BLAST152
Regioni390 – 415Zinc finger-like 1Add BLAST26
Regioni783 – 802Zinc finger-like 2By similarityAdd BLAST20
Regioni816 – 836Zinc finger-like 3By similarityAdd BLAST21

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili104 – 142Sequence analysisAdd BLAST39

Domaini

Each zinc finger-like domain binds a zinc ion and is involved in both ssDNA and dsDNA binding, as is the OB-fold domain.By similarity
The N-terminal domain mediates homodimerization.By similarity

Sequence similaritiesi

Belongs to the MCM10 family.Curated

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG3056 Eukaryota
ENOG41100ZT LUCA
GeneTreeiENSGT00390000007134
HOVERGENiHBG055312
InParanoidiQ7L590
KOiK10736
OMAiVACLSID
OrthoDBiEOG091G053R
PhylomeDBiQ7L590
TreeFamiTF313330

Family and domain databases

InterProiView protein in InterPro
IPR015411 Rep_factor_Mcm10_C
IPR015408 Znf_Mcm10/DnaG
PfamiView protein in Pfam
PF09332 Mcm10, 1 hit
PF09329 zf-primase, 1 hit
SMARTiView protein in SMART
SM01280 Mcm10, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7L590-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDEEEDNLSL LTALLEENES ALDCNSEENN FLTRENGEPD AFDELFDADG 
        60         70         80         90        100
DGESYTEEAD DGETGETRDE KENLATLFGD MEDLTDEEEV PASQSTENRV 
       110        120        130        140        150
LPAPAPRREK TNEELQEELR NLQEQMKALQ EQLKVTTIKQ TASPARLQKS 
       160        170        180        190        200
PVEKSPRPPL KERRVQRIQE STCFSAELDV PALPRTKRVA RTPKASPPDP 
       210        220        230        240        250
KSSSSRMTSA PSQPLQTISR NKPSGITRGQ IVGTPGSSGE TTQPICVEAF 
       260        270        280        290        300
SGLRLRRPRV SSTEMNKKMT GRKLIRLSQI KEKMAREKLE EIDWVTFGVI 
       310        320        330        340        350
LKKVTPQSVN SGKTFSIWKL NDLRDLTQCV SLFLFGEVHK ALWKTEQGTV 
       360        370        380        390        400
VGILNANPMK PKDGSEEVCL SIDHPQKVLI MGEALDLGTC KAKKKNGEPC 
       410        420        430        440        450
TQTVNLRDCE YCQYHVQAQY KKLSAKRADL QSTFSGGRIP KKFARRGTSL 
       460        470        480        490        500
KERLCQDGFY YGGVSSASYA ASIAAAVAPK KKIQTTLSNL VVKGTNLIIQ 
       510        520        530        540        550
ETRQKLGIPQ KSLSCSEEFK ELMDLPTCGA RNLKQHLAKA TASGIMGSPK 
       560        570        580        590        600
PAIKSISASA LLKQQKQRML EMRRRKSEEI QKRFLQSSSE VESPAVPSSS 
       610        620        630        640        650
RQPPAQPPRT GSEFPRLEGA PATMTPKLGR GVLEGDDVLF YDESPPPRPK 
       660        670        680        690        700
LSALAEAKKL AAITKLRAKG QVLTKTNPNS IKKKQKDPQD ILEVKERVEK 
       710        720        730        740        750
NTMFSSQAED ELEPARKKRR EQLAYLESEE FQKILKAKSK HTGILKEAEA 
       760        770        780        790        800
EMQERYFEPL VKKEQMEEKM RNIREVKCRV VTCKTCAYTH FKLLETCVSE 
       810        820        830        840        850
QHEYHWHDGV KRFFKCPCGN RSISLDRLPN KHCSNCGLYK WERDGMLKEK 
       860        870 
TGPKIGGETL LPRGEEHAKF LNSLK
Length:875
Mass (Da):98,183
Last modified:February 20, 2007 - v2
Checksum:iDD87191FFF3729D4
GO
Isoform 2 (identifier: Q7L590-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     152-152: Missing.

Show »
Length:874
Mass (Da):98,084
Checksum:i50A89BAF82253B43
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5T670Q5T670_HUMAN
MCM10 minichromosome maintenance de...
MCM10 hCG_40780
855Annotation score:
C9J600C9J600_HUMAN
Protein MCM10 homolog
MCM10
69Annotation score:

Sequence cautioni

The sequence AAF69623 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti303K → R in BAB70988 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_030771134K → R1 PublicationCorresponds to variant dbSNP:rs17152897Ensembl.1
Natural variantiVAR_053836195A → P2 PublicationsCorresponds to variant dbSNP:rs34630110Ensembl.1
Natural variantiVAR_053837418A → V. Corresponds to variant dbSNP:rs35114749Ensembl.1
Natural variantiVAR_030772541T → S2 PublicationsCorresponds to variant dbSNP:rs7905784Ensembl.1
Natural variantiVAR_030773669K → R1 PublicationCorresponds to variant dbSNP:rs2274110Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_029951152Missing in isoform 2. 3 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042719 mRNA Translation: BAB18723.1
AL136840 mRNA Translation: CAB66774.2
AK055695 mRNA Translation: BAB70988.1
AK292701 mRNA Translation: BAF85390.1
AL355355 Genomic DNA No translation available.
AL138764 Genomic DNA No translation available.
CH471072 Genomic DNA Translation: EAW86297.1
BC004876 mRNA Translation: AAH04876.2
BC101727 mRNA Translation: AAI01728.1
BC143490 mRNA Translation: AAI43491.1
AF119869 mRNA Translation: AAF69623.1 Different initiation.
CCDSiCCDS7095.1 [Q7L590-2]
CCDS7096.1 [Q7L590-1]
RefSeqiNP_060988.3, NM_018518.4 [Q7L590-2]
NP_877428.1, NM_182751.2 [Q7L590-1]
XP_011517840.1, XM_011519538.2 [Q7L590-1]
UniGeneiHs.198363

Genome annotation databases

EnsembliENST00000378714; ENSP00000367986; ENSG00000065328 [Q7L590-2]
ENST00000484800; ENSP00000418268; ENSG00000065328 [Q7L590-1]
GeneIDi55388
KEGGihsa:55388
UCSCiuc001ima.4 human [Q7L590-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042719 mRNA Translation: BAB18723.1
AL136840 mRNA Translation: CAB66774.2
AK055695 mRNA Translation: BAB70988.1
AK292701 mRNA Translation: BAF85390.1
AL355355 Genomic DNA No translation available.
AL138764 Genomic DNA No translation available.
CH471072 Genomic DNA Translation: EAW86297.1
BC004876 mRNA Translation: AAH04876.2
BC101727 mRNA Translation: AAI01728.1
BC143490 mRNA Translation: AAI43491.1
AF119869 mRNA Translation: AAF69623.1 Different initiation.
CCDSiCCDS7095.1 [Q7L590-2]
CCDS7096.1 [Q7L590-1]
RefSeqiNP_060988.3, NM_018518.4 [Q7L590-2]
NP_877428.1, NM_182751.2 [Q7L590-1]
XP_011517840.1, XM_011519538.2 [Q7L590-1]
UniGeneiHs.198363

3D structure databases

ProteinModelPortaliQ7L590
SMRiQ7L590
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120654, 67 interactors
IntActiQ7L590, 40 interactors
MINTiQ7L590
STRINGi9606.ENSP00000354945

PTM databases

iPTMnetiQ7L590
PhosphoSitePlusiQ7L590

Polymorphism and mutation databases

BioMutaiMCM10
DMDMi126215746

Proteomic databases

EPDiQ7L590
MaxQBiQ7L590
PaxDbiQ7L590
PeptideAtlasiQ7L590
PRIDEiQ7L590
ProteomicsDBi68795
68796 [Q7L590-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378714; ENSP00000367986; ENSG00000065328 [Q7L590-2]
ENST00000484800; ENSP00000418268; ENSG00000065328 [Q7L590-1]
GeneIDi55388
KEGGihsa:55388
UCSCiuc001ima.4 human [Q7L590-1]

Organism-specific databases

CTDi55388
DisGeNETi55388
EuPathDBiHostDB:ENSG00000065328.16
GeneCardsiMCM10
HGNCiHGNC:18043 MCM10
HPAiHPA045899
HPA071952
MIMi609357 gene
neXtProtiNX_Q7L590
OpenTargetsiENSG00000065328
PharmGKBiPA30689
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3056 Eukaryota
ENOG41100ZT LUCA
GeneTreeiENSGT00390000007134
HOVERGENiHBG055312
InParanoidiQ7L590
KOiK10736
OMAiVACLSID
OrthoDBiEOG091G053R
PhylomeDBiQ7L590
TreeFamiTF313330

Enzyme and pathway databases

ReactomeiR-HSA-176187 Activation of ATR in response to replication stress
R-HSA-68962 Activation of the pre-replicative complex
SIGNORiQ7L590

Miscellaneous databases

ChiTaRSiMCM10 human
GeneWikiiMCM10
GenomeRNAii55388
PROiPR:Q7L590
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000065328 Expressed in 110 organ(s), highest expression level in amniotic fluid
CleanExiHS_MCM10
ExpressionAtlasiQ7L590 baseline and differential
GenevisibleiQ7L590 HS

Family and domain databases

InterProiView protein in InterPro
IPR015411 Rep_factor_Mcm10_C
IPR015408 Znf_Mcm10/DnaG
PfamiView protein in Pfam
PF09332 Mcm10, 1 hit
PF09329 zf-primase, 1 hit
SMARTiView protein in SMART
SM01280 Mcm10, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMCM10_HUMAN
AccessioniPrimary (citable) accession number: Q7L590
Secondary accession number(s): A8K9I6
, B7ZKZ8, Q3MIR3, Q7LD55, Q96GX4, Q96NB6, Q9H0D7, Q9H3P9, Q9P177
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
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