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Protein

7SK snRNA methylphosphate capping enzyme

Gene

MEPCE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei581S-adenosyl-L-methionine; via carbonyl oxygen1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
7SK snRNA methylphosphate capping enzyme (EC:2.1.1.-)
Short name:
MePCE
Alternative name(s):
Bicoid-interacting protein 3 homolog
Short name:
Bin3 homolog
Gene namesi
Name:MEPCE
Synonyms:BCDIN3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000146834.13
HGNCiHGNC:20247 MEPCE
MIMi611478 gene
neXtProtiNX_Q7L2J0

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000146834
PharmGKBiPA162395768

Polymorphism and mutation databases

BioMutaiMEPCE
DMDMi74758999

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002892621 – 6897SK snRNA methylphosphate capping enzymeAdd BLAST689

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei57PhosphoserineCombined sources1
Modified residuei60PhosphoserineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei101PhosphoserineCombined sources1
Modified residuei117Omega-N-methylarginineBy similarity1
Modified residuei152PhosphoserineCombined sources1
Modified residuei175PhosphoserineCombined sources1
Modified residuei179PhosphoserineCombined sources1
Modified residuei213PhosphothreonineCombined sources1
Modified residuei216PhosphoserineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Modified residuei254PhosphoserineCombined sources1
Modified residuei291PhosphothreonineCombined sources1
Modified residuei330PhosphoserineCombined sources1
Modified residuei344PhosphoserineCombined sources1
Modified residuei390PhosphoserineCombined sources1
Cross-linki643Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ7L2J0
MaxQBiQ7L2J0
PaxDbiQ7L2J0
PeptideAtlasiQ7L2J0
PRIDEiQ7L2J0
ProteomicsDBi68762

PTM databases

iPTMnetiQ7L2J0
PhosphoSitePlusiQ7L2J0

Expressioni

Tissue specificityi

Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues.1 Publication

Gene expression databases

BgeeiENSG00000146834 Expressed in 229 organ(s), highest expression level in right testis
CleanExiHS_MEPCE
GenevisibleiQ7L2J0 HS

Organism-specific databases

HPAiCAB026384
HPA051587

Interactioni

Subunit structurei

Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, MEPCE/BCDIN3, SART3 proteins and 7SK and U6 snRNAs (PubMed:17643375, Ref. 23). Interacts with METTL16 (PubMed:29051200).3 Publications

Protein-protein interaction databases

BioGridi121122, 194 interactors
IntActiQ7L2J0, 23 interactors
STRINGi9606.ENSP00000308546

Structurei

Secondary structure

1689
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ7L2J0
SMRiQ7L2J0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7L2J0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini431 – 686Bin3-type SAMPROSITE-ProRule annotationAdd BLAST256

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni451 – 453S-adenosyl-L-methionine binding3
Regioni474 – 475S-adenosyl-L-methionine binding2
Regioni559 – 560S-adenosyl-L-methionine binding2

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 161Gly-richAdd BLAST137

Sequence similaritiesi

Belongs to the methyltransferase superfamily.Curated

Phylogenomic databases

eggNOGiKOG2899 Eukaryota
ENOG410XZ7T LUCA
GeneTreeiENSGT00390000015611
HOGENOMiHOG000242946
HOVERGENiHBG099856
InParanoidiQ7L2J0
KOiK15190
OMAiSFFPMSM
OrthoDBiEOG091G08F2
PhylomeDBiQ7L2J0
TreeFamiTF324061

Family and domain databases

InterProiView protein in InterPro
IPR010675 Bin3_C
IPR024160 BIN3_SAM-bd_dom
IPR025714 Methyltranfer_dom
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF06859 Bin3, 1 hit
PF13847 Methyltransf_31, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51515 BIN3_SAM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q7L2J0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MIEMAAEKEP FLVPAPPPPL KDESGGGGGP TVPPHQEAAS GELRGGTERG
60 70 80 90 100
PGRCAPSAGS PAAAVGRESP GAAATSSSGP QAQQHRGGGP QAQSHGEARL
110 120 130 140 150
SDPPGRAAPP DVGEERRGGG GTELGPPAPP RPRNGYQPHR PPGGGGGKRR
160 170 180 190 200
NSCNVGGGGG GFKHPAFKRR RRVNSDCDSV LPSNFLLGGN IFDPLNLNSL
210 220 230 240 250
LDEEVSRTLN AETPKSSPLP AKGRDPVEIL IPKDITDPLS LNTCTDEGHV
260 270 280 290 300
VLASPLKTGR KRHRHRGQHH QQQQAAGGSE SHPVPPTAPL TPLLHGEGAS
310 320 330 340 350
QQPRHRGQNR DAPQPYELNT AINCRDEVVS PLPSALQGPS GSLSAPPAAS
360 370 380 390 400
VISAPPSSSS RHRKRRRTSS KSEAGARGGG QGSKEKGRGS WGGRHHHHHP
410 420 430 440 450
LPAAGFKKQQ RKFQYGNYCK YYGYRNPSCE DGRLRVLKPE WFRGRDVLDL
460 470 480 490 500
GCNVGHLTLS IACKWGPSRM VGLDIDSRLI HSARQNIRHY LSEELRLPPQ
510 520 530 540 550
TLEGDPGAEG EEGTTTVRKR SCFPASLTAS RGPIAAPQVP LDGADTSVFP
560 570 580 590 600
NNVVFVTGNY VLDRDDLVEA QTPEYDVVLC LSLTKWVHLN WGDEGLKRMF
610 620 630 640 650
RRIYRHLRPG GILVLEPQPW SSYGKRKTLT ETIYKNYYRI QLKPEQFSSY
660 670 680
LTSPDVGFSS YELVATPHNT SKGFQRPVYL FHKARSPSH
Length:689
Mass (Da):74,355
Last modified:July 5, 2004 - v1
Checksum:iCBB1D6BF144C923A
GO
Isoform 2 (identifier: Q7L2J0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-469: Missing.

Note: No experimental confirmation available.
Show »
Length:220
Mass (Da):24,950
Checksum:i799B7EB8BDF56524
GO

Sequence cautioni

The sequence AAF74767 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH16396 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA91040 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti661Y → N in BAG51598 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0445121 – 469Missing in isoform 2. 1 PublicationAdd BLAST469

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK055964 mRNA Translation: BAG51598.1
AC092849 Genomic DNA No translation available.
CH471091 Genomic DNA Translation: EAW76534.1
CH471091 Genomic DNA Translation: EAW76535.1
CH471091 Genomic DNA Translation: EAW76536.1
CH236956 Genomic DNA Translation: EAL23834.1
BC000556 mRNA Translation: AAH00556.2
BC016396 mRNA Translation: AAH16396.1 Different initiation.
BC018935 mRNA Translation: AAH18935.2
AF264752 Genomic DNA Translation: AAF74767.1 Different initiation.
AK000264 mRNA Translation: BAA91040.1 Different initiation.
CCDSiCCDS55136.1 [Q7L2J0-2]
CCDS5693.1 [Q7L2J0-1]
RefSeqiNP_001181919.1, NM_001194990.1 [Q7L2J0-2]
NP_001181920.1, NM_001194991.1 [Q7L2J0-2]
NP_001181921.1, NM_001194992.1 [Q7L2J0-2]
NP_062552.2, NM_019606.5 [Q7L2J0-1]
XP_011514712.1, XM_011516410.2 [Q7L2J0-2]
UniGeneiHs.178011

Genome annotation databases

EnsembliENST00000310512; ENSP00000308546; ENSG00000146834 [Q7L2J0-1]
ENST00000414441; ENSP00000400875; ENSG00000146834 [Q7L2J0-2]
GeneIDi56257
KEGGihsa:56257
UCSCiuc003uuv.4 human [Q7L2J0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK055964 mRNA Translation: BAG51598.1
AC092849 Genomic DNA No translation available.
CH471091 Genomic DNA Translation: EAW76534.1
CH471091 Genomic DNA Translation: EAW76535.1
CH471091 Genomic DNA Translation: EAW76536.1
CH236956 Genomic DNA Translation: EAL23834.1
BC000556 mRNA Translation: AAH00556.2
BC016396 mRNA Translation: AAH16396.1 Different initiation.
BC018935 mRNA Translation: AAH18935.2
AF264752 Genomic DNA Translation: AAF74767.1 Different initiation.
AK000264 mRNA Translation: BAA91040.1 Different initiation.
CCDSiCCDS55136.1 [Q7L2J0-2]
CCDS5693.1 [Q7L2J0-1]
RefSeqiNP_001181919.1, NM_001194990.1 [Q7L2J0-2]
NP_001181920.1, NM_001194991.1 [Q7L2J0-2]
NP_001181921.1, NM_001194992.1 [Q7L2J0-2]
NP_062552.2, NM_019606.5 [Q7L2J0-1]
XP_011514712.1, XM_011516410.2 [Q7L2J0-2]
UniGeneiHs.178011

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5UNAX-ray2.55A/B/C/D/E/F400-689[»]
ProteinModelPortaliQ7L2J0
SMRiQ7L2J0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121122, 194 interactors
IntActiQ7L2J0, 23 interactors
STRINGi9606.ENSP00000308546

PTM databases

iPTMnetiQ7L2J0
PhosphoSitePlusiQ7L2J0

Polymorphism and mutation databases

BioMutaiMEPCE
DMDMi74758999

Proteomic databases

EPDiQ7L2J0
MaxQBiQ7L2J0
PaxDbiQ7L2J0
PeptideAtlasiQ7L2J0
PRIDEiQ7L2J0
ProteomicsDBi68762

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310512; ENSP00000308546; ENSG00000146834 [Q7L2J0-1]
ENST00000414441; ENSP00000400875; ENSG00000146834 [Q7L2J0-2]
GeneIDi56257
KEGGihsa:56257
UCSCiuc003uuv.4 human [Q7L2J0-1]

Organism-specific databases

CTDi56257
EuPathDBiHostDB:ENSG00000146834.13
GeneCardsiMEPCE
HGNCiHGNC:20247 MEPCE
HPAiCAB026384
HPA051587
MIMi611478 gene
neXtProtiNX_Q7L2J0
OpenTargetsiENSG00000146834
PharmGKBiPA162395768
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2899 Eukaryota
ENOG410XZ7T LUCA
GeneTreeiENSGT00390000015611
HOGENOMiHOG000242946
HOVERGENiHBG099856
InParanoidiQ7L2J0
KOiK15190
OMAiSFFPMSM
OrthoDBiEOG091G08F2
PhylomeDBiQ7L2J0
TreeFamiTF324061

Miscellaneous databases

ChiTaRSiMEPCE human
EvolutionaryTraceiQ7L2J0
GenomeRNAii56257
PROiPR:Q7L2J0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000146834 Expressed in 229 organ(s), highest expression level in right testis
CleanExiHS_MEPCE
GenevisibleiQ7L2J0 HS

Family and domain databases

InterProiView protein in InterPro
IPR010675 Bin3_C
IPR024160 BIN3_SAM-bd_dom
IPR025714 Methyltranfer_dom
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF06859 Bin3, 1 hit
PF13847 Methyltransf_31, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51515 BIN3_SAM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMEPCE_HUMAN
AccessioniPrimary (citable) accession number: Q7L2J0
Secondary accession number(s): B3KP86, D6W5V7, Q9NPD4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 5, 2004
Last modified: October 10, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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