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Protein

Carbohydrate sulfotransferase 9

Gene

CHST9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Participates in biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Has a higher activity toward carbonic anhydrase VI than toward lutropin. Only active against terminal GalNAcbeta1,GalNAcbeta. Isoform 2, but not isoform 1, is active toward chondroitin.

Caution

It is uncertain whether Met-1 or Met-8 is the initiator.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi220 – 226PAPSBy similarity7
Nucleotide bindingi280 – 288PAPSBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-HSA-2022870 Chondroitin sulfate biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 9 (EC:2.8.2.-)
Alternative name(s):
GalNAc-4-O-sulfotransferase 2
Short name:
GalNAc-4-ST2
Short name:
GalNAc4ST-2
N-acetylgalactosamine-4-O-sulfotransferase 2
Gene namesi
Name:CHST9
ORF Names:UNQ2549/PRO6175
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000154080.12
HGNCiHGNC:19898 CHST9
MIMi610191 gene
neXtProtiNX_Q7L1S5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 33Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini34 – 443LumenalSequence analysisAdd BLAST410

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi83539
OpenTargetsiENSG00000154080
PharmGKBiPA134888782

Polymorphism and mutation databases

BioMutaiCHST9
DMDMi229462829

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001896551 – 443Carbohydrate sulfotransferase 9Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi159N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi243N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi324N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi437N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ7L1S5
PeptideAtlasiQ7L1S5
PRIDEiQ7L1S5
ProteomicsDBi68748
68749 [Q7L1S5-2]

PTM databases

iPTMnetiQ7L1S5
PhosphoSitePlusiQ7L1S5

Expressioni

Tissue specificityi

Highly expressed in trachea. Also expressed in fetal lung, adult pancreas, testis and salivary gland. Expressed at low level in pituitary gland, apex of the heart, adult lung, prostate and mammary gland. Weakly or not expressed in heart, liver and spinal cord.2 Publications

Gene expression databases

BgeeiENSG00000154080
CleanExiHS_CHST9
ExpressionAtlasiQ7L1S5 baseline and differential
GenevisibleiQ7L1S5 HS

Organism-specific databases

HPAiHPA011393

Interactioni

Protein-protein interaction databases

BioGridi123672, 4 interactors
STRINGi9606.ENSP00000284224

Structurei

3D structure databases

ProteinModelPortaliQ7L1S5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4651 Eukaryota
ENOG4111GJR LUCA
GeneTreeiENSGT00760000119214
HOGENOMiHOG000231801
HOVERGENiHBG050950
InParanoidiQ7L1S5
KOiK09673
OMAiNAQVVRQ
OrthoDBiEOG091G0FZO
PhylomeDBiQ7L1S5
TreeFamiTF325581

Family and domain databases

InterProiView protein in InterPro
IPR018011 Carb_sulfotrans_8-10
IPR005331 Sulfotransferase
PANTHERiPTHR12137 PTHR12137, 1 hit
PfamiView protein in Pfam
PF03567 Sulfotransfer_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7L1S5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQPSEMVMNP KQVFLSVLIF GVAGLLLFMY LQVWIEEQHT GRVEKRREQK
60 70 80 90 100
VTSGWGPVKY LRPVPRIMST EKIQEHITNQ NPKFHMPEDV REKKENLLLN
110 120 130 140 150
SERSTRLLTK TSHSQGGDQA LSKSTGSPTE KLIEKRQGAK TVFNKFSNMN
160 170 180 190 200
WPVDIHPLNK SLVKDNKWKK TEETQEKRRS FLQEFCKKYG GVSHHQSHLF
210 220 230 240 250
HTVSRIYVED KHKILYCEVP KAGCSNWKRI LMVLNGLASS AYNISHNAVH
260 270 280 290 300
YGKHLKKLDS FDLKGIYTRL NTYTKAVFVR DPMERLVSAF RDKFEHPNSY
310 320 330 340 350
YHPVFGKAII KKYRPNACEE ALINGSGVKF KEFIHYLLDS HRPVGMDIHW
360 370 380 390 400
EKVSKLCYPC LINYDFVGKF ETLEEDANYF LQMIGAPKEL KFPNFKDRHS
410 420 430 440
SDERTNAQVV RQYLKDLTRT ERQLIYDFYY LDYLMFNYTT PFL
Length:443
Mass (Da):52,055
Last modified:May 5, 2009 - v2
Checksum:i5455E67D20C9F8D6
GO
Isoform 2 (identifier: Q7L1S5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     68-74: MSTEKIQ → KPQVSHA
     75-443: Missing.

Show »
Length:74
Mass (Da):8,591
Checksum:i1F1A2CBAD277FA16
GO

Sequence cautioni

The sequence AAH25764 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAK01862 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAK30370 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136R → H in AAK01862 (PubMed:11445554).Curated1
Sequence conflicti150N → D in AAK01862 (PubMed:11445554).Curated1
Sequence conflicti194 – 195HH → PP in AAK30370 (PubMed:11139592).Curated2
Sequence conflicti442F → L in AAQ88852 (PubMed:12975309).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055150122S → N. Corresponds to variant dbSNP:rs17694469Ensembl.1
Natural variantiVAR_079095378N → S1 PublicationCorresponds to variant dbSNP:rs758130927Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04386568 – 74MSTEKIQ → KPQVSHA in isoform 2. 1 Publication7
Alternative sequenceiVSP_04386675 – 443Missing in isoform 2. 1 PublicationAdd BLAST369

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239821 mRNA Translation: AAK01862.1 Different initiation.
AF332472 mRNA Translation: AAK30369.1
AF332473 mRNA Translation: AAK30370.1 Sequence problems.
AY358488 mRNA Translation: AAQ88852.1
AK093349 mRNA Translation: BAC04141.1
AC010854 Genomic DNA No translation available.
AC023575 Genomic DNA No translation available.
AC116017 Genomic DNA No translation available.
BC025764 mRNA Translation: AAH25764.2 Different initiation.
CCDSiCCDS42422.1 [Q7L1S5-1]
CCDS58618.1 [Q7L1S5-2]
RefSeqiNP_001243245.1, NM_001256316.1 [Q7L1S5-2]
NP_113610.2, NM_031422.5 [Q7L1S5-1]
XP_016881522.1, XM_017026033.1 [Q7L1S5-1]
UniGeneiHs.44584
Hs.706552

Genome annotation databases

EnsembliENST00000580774; ENSP00000464655; ENSG00000154080 [Q7L1S5-2]
ENST00000581714; ENSP00000462852; ENSG00000154080 [Q7L1S5-1]
ENST00000618847; ENSP00000480991; ENSG00000154080 [Q7L1S5-1]
GeneIDi83539
KEGGihsa:83539
UCSCiuc002kwd.5 human [Q7L1S5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCHST9_HUMAN
AccessioniPrimary (citable) accession number: Q7L1S5
Secondary accession number(s): Q6UX69
, Q9BXH3, Q9BXH4, Q9BZW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: May 5, 2009
Last modified: June 20, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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