Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 137 (11 Dec 2019)
Sequence version 1 (03 Oct 2006)
Previous versions | rss
Help videoAdd a publicationFeedback
Protein
Submitted name:

Apaf-1 related killer DARK

Gene

Dark

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Submitted name:
Apaf-1 related killer DARKImported
Submitted name:
Apaf-1/CED-4-related caspase activator Dapaf-1LImported
Submitted name:
Cell death protein HAC-1Imported
Submitted name:
Death-associated APAF1-related killer, isoform BImported
Submitted name:
FI21208p1Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:DarkImported
Synonyms:anon-53FaImported, ApafImported, APAF-1Imported, Apaf-1Imported, apaf-1Imported, Apaf-IImported, APAF1Imported, Apaf1Imported, apaf1Imported, arcImported, ARKImported, ArkImported, arkImported, Ark-RBImported, D-Apaf-1Imported, dapafImported, Dapaf-1Imported, dAPAF-1Imported, dApaf-1Imported, dapaf-1Imported, dapaf-1LImported, dapaf-1SImported, dApaf1Imported, DARKImported, dArkImported, darkImported, Dmel\CG6829Imported, HAC-1Imported, Hac-1Imported, hac-1Imported, HAC1Imported, Hac1Imported, hac1Imported, l(2)SH0173Imported, T1Imported
ORF Names:CG6829Imported, Dmel_CG6829Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2R

Organism-specific databases

Drosophila genome database

More...
FlyBasei
FBgn0263864 Dark

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
FBgn0263864 Expressed in 22 organ(s), highest expression level in head mesoderm (Drosophila)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
Q7KLI1, 2 interactors

STRING: functional protein association networks

More...
STRINGi
7227.FBpp0086122

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini135 – 303NB-ARCInterPro annotationAdd BLAST169

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1269 – 1289DisorderedSequence analysisAdd BLAST21

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG4658 Eukaryota
COG4886 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000157710

KEGG Orthology (KO)

More...
KOi
K02084

Identification of Orthologs from Complete Genome Data

More...
OMAi
NLGCKIL

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.130.10.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002182 NB-ARC
IPR027417 P-loop_NTPase
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR036322 WD40_repeat_dom_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00931 NB-ARC, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00320 WD40, 5 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50978 SSF50978, 2 hits
SSF52540 SSF52540, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q7KLI1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDFETGEHQY QYKDILSVFE DAFVDNFDCK DVQDMPKSIL SKEEIDHIIM
60 70 80 90 100
SKDAVSGTLR LFWTLLSKQE EMVQKFVEEV LRINYKFLMS PIKTEQRQPS
110 120 130 140 150
MMTRMYIEQR DRLYNDNQVF AKYNVSRLQP YLKLRQALLE LRPAKNVLID
160 170 180 190 200
GVLGSGKTWV ALDVCLSYKV QCKMDFKIFW LNLKNCNSPE TVLEMLQKLL
210 220 230 240 250
YQIDPNWTSR SDHSSNIKLR IHSIQAELRR LLKSKPYENC LLVLLNVQNA
260 270 280 290 300
KAWNAFNLSC KILLTTRFKQ VTDFLSAATT THISLDHHSM TLTPDEVKSL
310 320 330 340 350
LLKYLDCRPQ DLPREVLTTN PRRLSIIAES IRDGLATWDN WKHVNCDKLT
360 370 380 390 400
TIIESSLNVL EPAEYRKMFD RLSVFPPSAH IPTILLSLIW FDVIKSDVMV
410 420 430 440 450
VVNKLHKYSL VEKQPKESTI SIPSIYLELK VKLENEYALH RSIVDHYNIP
460 470 480 490 500
KTFDSDDLIP PYLDQYFYSH IGHHLKNIEH PERMTLFRMV FLDFRFLEQK
510 520 530 540 550
IRHDSTAWNA SGSILNTLQQ LKFYKPYICD NDPKYERLVN AILDFLPKIE
560 570 580 590 600
ENLICSKYTD LLRIALMAED EAIFEEAHKQ VQRFDDRVWF TNHGRFHQHR
610 620 630 640 650
QIINLGDNEG RHAVYLHNDF CLIALASGQI LLTDVSLEGE DTYLLRDESD
660 670 680 690 700
SSDILRMAVF NQQKHLITLH CNGSVKLWSL WPDCPGRRHS GGSKQQLVNS
710 720 730 740 750
VVKRFIGSYA NLKIVAFYLN EDAGLPEANI QLHVAFINGD VSILNWDEQD
760 770 780 790 800
QEFKLSHVPV LKTMQSGIRC FVQVLKRYYV VCTSNCTLTV WDLTNGSSNT
810 820 830 840 850
LELHVFNVEN DTPLALDVFD ERSKTATVLL IFKYSVWRLN FLPGLSVSLQ
860 870 880 890 900
SEAVQLPEGS FITCGKRSTD GRYLLLGTSE GLIVYDLKIS DPVLRSNVSE
910 920 930 940 950
HIECVDIYEL FDPVYKYIVL CGAKGKQVVH VHTLRSVSGS NSHQNREIAW
960 970 980 990 1000
VHSADEISVM TKACLEPNVY LRSLMDMTRE RTQLLAVDSK ERIHLIKPAI
1010 1020 1030 1040 1050
SRISEWSTIT PTHAASNCKI NAISAFNDEQ IFVGYVDGVI IDVIHDTALP
1060 1070 1080 1090 1100
QQFIEEPIDY LKQVSPNILV ASAHSAQKTV IFQLEKIDPL QPNDQWPLMM
1110 1120 1130 1140 1150
DVSTKYASLQ EGQYIILFSD HGVCHLDIAN PSAFVKPKDS EEYIVGFDLK
1160 1170 1180 1190 1200
NSLLFLAYEN NIIDVFRLIF SCNQLRYEQI CEEEIAQKAK ISYLVATDDG
1210 1220 1230 1240 1250
TMLAMGFENG TLELFAVENR KVQLIYSIEE VHEHCIRQLL FSPCKLLLIS
1260 1270 1280 1290 1300
CAEQLCFWNV THMRNNQLER EQKRRRSRRH KQHSVTQEDA VDAAPIAADI
1310 1320 1330 1340 1350
DVDVTFVADE FHPVNRGTAE LWRNKRGNAI RPELLACVKF VGNEARQFFT
1360 1370 1380 1390 1400
DAHFSHFYAI DDEGVYYHLQ LLELSRLQPP PDPVTLDIAN QYEDLKNLRI
1410 1420 1430 1440
LDSPLMQDSD SEGADVVGNL VLEKNGGVAR ATPILEEASS
Length:1,440
Mass (Da):165,619
Last modified:October 3, 2006 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i74EFEAD06E54FA74
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q9U8R4Q9U8R4_DROME
Apaf-1/CED-4-related caspase activa...
Dark anon-53Fa, Apaf, APAF-1, Apaf-1, apaf-1
531Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF162659 mRNA Translation: AAD45988.1
AF196306 mRNA Translation: AAF07207.2
AE013599 Genomic DNA Translation: AAF57916.1
BT150397 mRNA Translation: AHA95701.1
AB027531 mRNA Translation: BAA86939.1

NCBI Reference Sequences

More...
RefSeqi
NP_725637.1, NM_166207.3

Genome annotation databases

Ensembl metazoan genome annotation project

More...
EnsemblMetazoai
FBtr0086968; FBpp0086122; FBgn0263864

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
36914

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
dme:Dmel_CG6829

UCSC genome browser

More...
UCSCi
CG6829-RB d. melanogaster

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF162659 mRNA Translation: AAD45988.1
AF196306 mRNA Translation: AAF07207.2
AE013599 Genomic DNA Translation: AAF57916.1
BT150397 mRNA Translation: AHA95701.1
AB027531 mRNA Translation: BAA86939.1
RefSeqiNP_725637.1, NM_166207.3

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J9Kelectron microscopy4.10A/C/E/G/I/K/M/O/Q/S/U/W/Y/a/c/e1-583[»]
3J9Lelectron microscopy4.00A/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q1-583[»]
4V4Lelectron microscopy6.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-583[»]
5JULelectron microscopy4.40A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-1440[»]
ModBaseiSearch...
SWISS-MODEL-WorkspaceiSubmit a new modelling project...
PDBe-KBiSearch...

Protein-protein interaction databases

IntActiQ7KLI1, 2 interactors
STRINGi7227.FBpp0086122

Genome annotation databases

EnsemblMetazoaiFBtr0086968; FBpp0086122; FBgn0263864
GeneIDi36914
KEGGidme:Dmel_CG6829
UCSCiCG6829-RB d. melanogaster

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
36914
FlyBaseiFBgn0263864 Dark

Phylogenomic databases

eggNOGiKOG4658 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00940000157710
KOiK02084
OMAiNLGCKIL

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Dark fly

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
36914

Gene expression databases

BgeeiFBgn0263864 Expressed in 22 organ(s), highest expression level in head mesoderm (Drosophila)

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR002182 NB-ARC
IPR027417 P-loop_NTPase
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00931 NB-ARC, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 2 hits
SSF52540 SSF52540, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiQ7KLI1_DROME
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q7KLI1
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: October 3, 2006
Last sequence update: October 3, 2006
Last modified: December 11, 2019
This is version 137 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywords - Technical termi

3D-structureCombined sources, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again