UniProtKB - Q7GB25 (AB5C_ARATH)
Protein
ABC transporter C family member 5
Gene
ABCC5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Pump for glutathione S-conjugates. Involved in regulation of K+ and Na+ cell content. Mediates resistance to NaCl and Li+, confers sensitivity to sulfonylurea drugs such as glibenclamide (inducer of stomatal opening), and required for stomatal opening regulation by auxin, abscisic acid (ABA) and external Ca2+. Transports oestradiol-17-(beta-D-glucuronide) (E217G). Involved in the root auxin content regulation that controls the transition from primary root elongation to lateral root formation. Plays a role in ABA-mediated germination inhibition (PubMed:11296221, PubMed:11855639, PubMed:12943546, PubMed:14684837). High-affinity inositol hexakisphosphate transporter that plays a role in guard cell signaling and phytic acid storage. Required for phytic acid accumulation in developing seeds. Phytic acid is the primary storage form of phosphorus in cereal grains and other plant seeds (PubMed:19797057).5 Publications
Catalytic activityi
- ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2). EC:7.6.2.2
Activity regulationi
(E217G) transport activity in negatively regulated by organic anions such as oestradiol-3-sulfate, luteolin-7-O-diglucuronide-4'-O-glucuronide, glycocholate, vanadate and the sulfonylurea glibenclamide, and, to a lower extent, by bafilomycin A1, NH4Cl, GSH, GSSG and DNB-GS.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 657 – 664 | ATP 1PROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 1302 – 1309 | ATP 2PROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATPase-coupled transmembrane transporter activity Source: TAIR
- ATPase-coupled xenobiotic transmembrane transporter activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- sulfonylurea receptor activity Source: TAIR
GO - Biological processi
- abscisic acid-activated signaling pathway involved in stomatal movement Source: TAIR
- cellular potassium ion homeostasis Source: TAIR
- response to salt stress Source: TAIR
- transmembrane transport Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT1G04120-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.208.21, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: ABC transporter C family member 5 (EC:7.6.2.2)Short name: ABC transporter ABCC.5 Short name: AtABCC5 Alternative name(s): ATP-energized glutathione S-conjugate pump 5 Glutathione S-conjugate-transporting ATPase 5 Multidrug resistance-associated protein 5 |
Gene namesi | Name:ABCC5 Synonyms:MRP5 Ordered Locus Names:At1g04120 ORF Names:F20D22.11 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G04120 |
TAIRi | locus:2020235, AT1G04120 |
Subcellular locationi
Other locations
- Membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation
Vacuole
- plant-type vacuole Source: TAIR
- vacuolar membrane Source: TAIR
- vacuole Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 16 – 36 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 76 – 96 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 111 – 131 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 142 – 162 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 177 – 197 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 312 – 332 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 334 – 354 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 421 – 441 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 446 – 466 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 533 – 553 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 946 – 966 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 986 – 1006 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1078 – 1098 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1117 – 1137 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1155 – 1175 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1180 – 1200 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000226076 | 1 – 1514 | ABC transporter C family member 5Add BLAST | 1514 |
Proteomic databases
PaxDbi | Q7GB25 |
PRIDEi | Q7GB25 |
ProteomicsDBi | 245093 [Q7GB25-1] |
PTM databases
iPTMneti | Q7GB25 |
Expressioni
Tissue specificityi
Ubiquitous, mostly in vascular tissues and epidermis, including guard cells.3 Publications
Developmental stagei
In flowers, present in anthers central vascular strand of the filament and in connecting tissues of the pollen sacs. Also present at the silique attachment site of the pedicel.1 Publication
Gene expression databases
ExpressionAtlasi | Q7GB25, baseline and differential |
Genevisiblei | Q7GB25, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 24512, 7 interactors |
IntActi | Q7GB25, 7 interactors |
STRINGi | 3702.AT1G04120.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 307 – 588 | ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST | 282 | |
Domaini | 622 – 845 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 224 | |
Domaini | 949 – 1231 | ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST | 283 | |
Domaini | 1268 – 1502 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 235 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 899 – 927 | Sequence analysisAdd BLAST | 29 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification]Curated
Keywords - Domaini
Coiled coil, Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0054, Eukaryota |
HOGENOMi | CLU_000604_27_3_1 |
InParanoidi | Q7GB25 |
PhylomeDBi | Q7GB25 |
Family and domain databases
Gene3Di | 1.20.1560.10, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q7GB25-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDFIEISLIF REHLPLLELC SVIINLLLFL VFLFAVSARQ ILVCVRRGRD
60 70 80 90 100
RLSKDDTVSA SNLSLEREVN HVSVGFGFNL SLLCCLYVLG VQVLVLVYDG
110 120 130 140 150
VKVRREVSDW FVLCFPASQS LAWFVLSFLV LHLKYKSSEK LPFLVRIWWF
160 170 180 190 200
LAFSICLCTM YVDGRRLAIE GWSRCSSHVV ANLAVTPALG FLCFLAWRGV
210 220 230 240 250
SGIQVTRSSS DLQEPLLVEE EAACLKVTPY STAGLVSLIT LSWLDPLLSA
260 270 280 290 300
GSKRPLELKD IPLLAPRDRA KSSYKVLKSN WKRCKSENPS KPPSLARAIM
310 320 330 340 350
KSFWKEAACN AVFAGLNTLV SYVGPYLISY FVDYLGGKEI FPHEGYVLAG
360 370 380 390 400
IFFTSKLIET VTTRQWYMGV DILGMHVRSA LTAMVYRKGL KLSSIAKQNH
410 420 430 440 450
TSGEIVNYMA VDVQRIGDYS WYLHDIWMLP MQIVLALAIL YKSVGIAAVA
460 470 480 490 500
TLVATIISIL VTIPLAKVQE DYQDKLMTAK DERMRKTSEC LRNMRVLKLQ
510 520 530 540 550
AWEDRYRVRL EEMREEEYGW LRKALYSQAF VTFIFWSSPI FVAAVTFATS
560 570 580 590 600
IFLGTQLTAG GVLSALATFR ILQEPLRNFP DLVSMMAQTK VSLDRISGFL
610 620 630 640 650
QEEELQEDAT VVIPRGLSNI AIEIKDGVFC WDPFSSRPTL SGIQMKVEKG
660 670 680 690 700
MRVAVCGTVG SGKSSFISCI LGEIPKISGE VRICGTTGYV SQSAWIQSGN
710 720 730 740 750
IEENILFGSP MEKTKYKNVI QACSLKKDIE LFSHGDQTII GERGINLSGG
760 770 780 790 800
QKQRVQLARA LYQDADIYLL DDPFSALDAH TGSDLFRDYI LSALAEKTVV
810 820 830 840 850
FVTHQVEFLP AADLILVLKE GRIIQSGKYD DLLQAGTDFK ALVSAHHEAI
860 870 880 890 900
EAMDIPSPSS EDSDENPIRD SLVLHNPKSD VFENDIETLA KEVQEGGSAS
910 920 930 940 950
DLKAIKEKKK KAKRSRKKQL VQEEERVKGK VSMKVYLSYM GAAYKGALIP
960 970 980 990 1000
LIILAQAAFQ FLQIASNWWM AWANPQTEGD ESKVDPTLLL IVYTALAFGS
1010 1020 1030 1040 1050
SVFIFVRAAL VATFGLAAAQ KLFLNMLRSV FRAPMSFFDS TPAGRILNRV
1060 1070 1080 1090 1100
SIDQSVVDLD IPFRLGGFAS TTIQLCGIVA VMTNVTWQVF LLVVPVAVAC
1110 1120 1130 1140 1150
FWMQKYYMAS SRELVRIVSI QKSPIIHLFG ESIAGAATIR GFGQEKRFIK
1160 1170 1180 1190 1200
RNLYLLDCFV RPFFCSIAAI EWLCLRMELL STLVFAFCMV LLVSFPHGTI
1210 1220 1230 1240 1250
DPSMAGLAVT YGLNLNGRLS RWILSFCKLE NKIISIERIY QYSQIVGEAP
1260 1270 1280 1290 1300
AIIEDFRPPS SWPATGTIEL VDVKVRYAEN LPTVLHGVSC VFPGGKKIGI
1310 1320 1330 1340 1350
VGRTGSGKST LIQALFRLIE PTAGKITIDN IDISQIGLHD LRSRLGIIPQ
1360 1370 1380 1390 1400
DPTLFEGTIR ANLDPLEEHS DDKIWEALDK SQLGDVVRGK DLKLDSPVLE
1410 1420 1430 1440 1450
NGDNWSVGQR QLVSLGRALL KQAKILVLDE ATASVDTATD NLIQKIIRTE
1460 1470 1480 1490 1500
FEDCTVCTIA HRIPTVIDSD LVLVLSDGRV AEFDTPARLL EDKSSMFLKL
1510
VTEYSSRSTG IPEL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4I454 | F4I454_ARATH | Multidrug resistance-associated pro... | ABCC5 ATABCC5, ATMRP5, ATP-binding cassette C5, MULTIDRUG RESISTANCE PROTEIN 5, At1g04120 | 1,509 | Annotation score: | ||
A0A1P8AT08 | A0A1P8AT08_ARATH | Multidrug resistance-associated pro... | ABCC5 ATABCC5, ATMRP5, ATP-binding cassette C5, MULTIDRUG RESISTANCE PROTEIN 5, At1g04120 | 1,479 | Annotation score: |
Sequence cautioni
The sequence AAC16754 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y11250 mRNA Translation: CAA72120.1 AF225908 mRNA Translation: AAG14965.1 AC002411 Genomic DNA Translation: AAC16754.1 Sequence problems. CP002684 Genomic DNA Translation: AEE27658.1 AK220840 mRNA Translation: BAD94167.1 |
PIRi | T00961 T52080 |
RefSeqi | NP_171908.1, NM_100293.3 [Q7GB25-1] |
Genome annotation databases
EnsemblPlantsi | AT1G04120.1; AT1G04120.1; AT1G04120 [Q7GB25-1] |
GeneIDi | 839277 |
Gramenei | AT1G04120.1; AT1G04120.1; AT1G04120 [Q7GB25-1] |
KEGGi | ath:AT1G04120 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y11250 mRNA Translation: CAA72120.1 AF225908 mRNA Translation: AAG14965.1 AC002411 Genomic DNA Translation: AAC16754.1 Sequence problems. CP002684 Genomic DNA Translation: AEE27658.1 AK220840 mRNA Translation: BAD94167.1 |
PIRi | T00961 T52080 |
RefSeqi | NP_171908.1, NM_100293.3 [Q7GB25-1] |
3D structure databases
SMRi | Q7GB25 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 24512, 7 interactors |
IntActi | Q7GB25, 7 interactors |
STRINGi | 3702.AT1G04120.1 |
Protein family/group databases
TCDBi | 3.A.1.208.21, the atp-binding cassette (abc) superfamily |
PTM databases
iPTMneti | Q7GB25 |
Proteomic databases
PaxDbi | Q7GB25 |
PRIDEi | Q7GB25 |
ProteomicsDBi | 245093 [Q7GB25-1] |
Genome annotation databases
EnsemblPlantsi | AT1G04120.1; AT1G04120.1; AT1G04120 [Q7GB25-1] |
GeneIDi | 839277 |
Gramenei | AT1G04120.1; AT1G04120.1; AT1G04120 [Q7GB25-1] |
KEGGi | ath:AT1G04120 |
Organism-specific databases
Araporti | AT1G04120 |
TAIRi | locus:2020235, AT1G04120 |
Phylogenomic databases
eggNOGi | KOG0054, Eukaryota |
HOGENOMi | CLU_000604_27_3_1 |
InParanoidi | Q7GB25 |
PhylomeDBi | Q7GB25 |
Enzyme and pathway databases
BioCyci | ARA:AT1G04120-MONOMER |
Miscellaneous databases
PROi | PR:Q7GB25 |
Gene expression databases
ExpressionAtlasi | Q7GB25, baseline and differential |
Genevisiblei | Q7GB25, AT |
Family and domain databases
Gene3Di | 1.20.1560.10, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AB5C_ARATH | |
Accessioni | Q7GB25Primary (citable) accession number: Q7GB25 Secondary accession number(s): O65619, Q56ZX0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 7, 2006 |
Last sequence update: | March 7, 2006 | |
Last modified: | August 12, 2020 | |
This is version 132 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families