UniProtKB - Q7G9P4 (ALDO3_ARATH)
Protein
Abscisic-aldehyde oxidase
Gene
AAO3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
In higher plants aldehyde oxidases (AO) appear to be homo- and heterodimeric assemblies of AO subunits with probably different physiological functions. AO-delta seems to be involved in the last step of abscisic acid biosynthesis, at least in leaves and seeds. In vitro, AO-delta oxidizes abscisic aldehyde to abscisic acid (ABA). In vitro, AO-delta also uses indole-3-aldehyde (IAld), benzaldehyde, 1-naphthaldehyde and cinnamaldehyde as substrate; the AAO2-AAO3 dimer also uses abscisic aldehyde as substrate.5 Publications
Miscellaneous
In vitro, cannot discriminate between (+) and (-) enantiomers of abscisic acid and leads respectively to (+) and (-) cis-ABA.
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 2 [2Fe-2S] clusters.By similarity
- FADBy similarity
- Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity
Kineticsi
All these kinetic values were obtained with AO-delta dimer.
- KM=0.51 µM for abscisic aldehyde1 Publication
- KM=34 µM for indole-3-aldehyde1 Publication
- KM=44 µM for benzaldehyde1 Publication
- KM=1.8 µM for 1-naphthaldehyde1 Publication
- KM=700 µM for cinnamaldehyde1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 40 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 45 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 48 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- abscisic aldehyde oxidase activity Source: TAIR
- aldehyde oxidase activity Source: TAIR
- electron transfer activity Source: InterPro
- FAD binding Source: InterPro
- indole-3-acetaldehyde oxidase activity Source: TAIR
- iron ion binding Source: InterPro
- ubiquitin protein ligase binding Source: UniProtKB
GO - Biological processi
- abscisic acid biosynthetic process Source: TAIR
- auxin biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Abscisic acid biosynthesis, Auxin biosynthesis |
Ligand | 2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD |
Enzyme and pathway databases
BioCyci | ARA:AT2G27150-MONOMER MetaCyc:AT2G27150-MONOMER |
BRENDAi | 1.2.3.14, 399 |
SABIO-RKi | Q7G9P4 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:AAO3 Synonyms:AO4 Ordered Locus Names:At2g27150 ORF Names:F20F1.2 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G27150 |
TAIRi | locus:2045149, AT2G27150 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Impaired abscisic acid (ABA) biosynthesis.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 821 – 826 | LQRPVK → WDLDQ in aao3-3; wilty phenotype in rosette leaves, reduced ABA levels, reduced dormancy, abnormal water loss and abnormal response to water deficit. 1 Publication | 6 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166111 | 1 – 1332 | Abscisic-aldehyde oxidaseAdd BLAST | 1332 |
Proteomic databases
PaxDbi | Q7G9P4 |
PRIDEi | Q7G9P4 |
ProteomicsDBi | 244792 |
PTM databases
iPTMneti | Q7G9P4 |
Expressioni
Tissue specificityi
Expressed in vascular tissues of all organs, particularly in phloem companion cells and xylem parenchymatic cells. Highly expressed in roots and rosettes, and to lower extent in seedlings, stems and flowers. Expressed at very low levels in siliques and dry seeds. Also detected in root dividing cells (tips and primordia), in mesophyll cells and inside the guard cells.7 Publications
Inductioni
Transcripts are induced by dehydration, in rosettes but not in roots. Induction by cold, ABA, sodium chloride (NaCl) and polyethylene glycol (PEG) is dependent of the zeaxanthin epoxidase ABA1 protein (ZEP). Induction by glucose requires the short chain alcohol dehydrogenase ABA2 protein. Repressed by mannitol.5 Publications
Gene expression databases
ExpressionAtlasi | Q7G9P4, baseline and differential |
Genevisiblei | Q7G9P4, AT |
Interactioni
Subunit structurei
Aldehyde oxidases (AO) are homodimers and heterodimers of AO subunits. AO-delta is a AAO3 homodimer. AAO3 also forms a dimer with AAO2.
Interacts with PUB44, and this interaction probably results in targeting of this protein to the proteasome.
3 PublicationsGO - Molecular functioni
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 3702.AT2G27150.2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 88 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 219 – 400 | FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST | 182 |
Sequence similaritiesi
Belongs to the xanthine dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | KOG0430, Eukaryota |
HOGENOMi | CLU_001681_1_1_1 |
InParanoidi | Q7G9P4 |
OrthoDBi | 48717at2759 |
PhylomeDBi | Q7G9P4 |
Family and domain databases
InterProi | View protein in InterPro IPR002888, 2Fe-2S-bd IPR036884, 2Fe-2S-bd_dom_sf IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR000674, Ald_Oxase/Xan_DH_a/b IPR036856, Ald_Oxase/Xan_DH_a/b_sf IPR016208, Ald_Oxase/xanthine_DH IPR008274, AldOxase/xan_DH_Mopterin-bd IPR037165, AldOxase/xan_DH_Mopterin-bd_sf IPR005107, CO_DH_flav_C IPR036683, CO_DH_flav_C_dom_sf IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR002346, Mopterin_DH_FAD-bd |
Pfami | View protein in Pfam PF01315, Ald_Xan_dh_C, 1 hit PF02738, Ald_Xan_dh_C2, 1 hit PF03450, CO_deh_flav_C, 1 hit PF00941, FAD_binding_5, 1 hit PF00111, Fer2, 1 hit PF01799, Fer2_2, 1 hit |
PIRSFi | PIRSF000127, Xanthine_DH, 1 hit |
SMARTi | View protein in SMART SM01008, Ald_Xan_dh_C, 1 hit SM01092, CO_deh_flav_C, 1 hit |
SUPFAMi | SSF47741, SSF47741, 1 hit SSF54292, SSF54292, 1 hit SSF54665, SSF54665, 1 hit SSF55447, SSF55447, 1 hit SSF56003, SSF56003, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit PS51387, FAD_PCMH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q7G9P4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLEFAVNGE RFKIDSVDPS TTLLEFLRLN TPFKSVKLGC GEGGCGACLV
60 70 80 90 100
VLSKYDPELD QVKECCINSC LTLLCSVNGC SITTSEGLGN TKKGFHPIHK
110 120 130 140 150
RFAGFHASQC GFCTPGMCIS LYSSLANAEN NSSKDFTVSE AEKSVSGNLC
160 170 180 190 200
RCTGYRPIVD ACKSFASDVD IEDLGLNSFW KKGESKEVMF KNLPPYNPKD
210 220 230 240 250
HLVTFPEFLK KKEKVDNGSD HLKYRWTTPF SVAELHNIME AANSGDSLKL
260 270 280 290 300
VVGNTGTGYY KDEERFDRYI DISNIPEMSM IKKDEKGIEI GAAVTISNAI
310 320 330 340 350
DALEKESKSS YVFKKMATHM EKIGNRSIRN SGSIGGNLVM AQSRKFPSDV
360 370 380 390 400
TTLLLAVDAS VYMLNGRKTE KVTLQEFLEL SPVLDSKRVL LKVEIPSWTA
410 420 430 440 450
PSGDDTEFLF ESYRAAPRSI GNALPYLNAA FLALVSRQEA SRKGVTVEKC
460 470 480 490 500
FLAFGSYGGD HSIRAIEVET FLTGKLLSYS VLYEAVGLLK GIIVPGKDTL
510 520 530 540 550
HSEYRKSLAV GYLFEFFYPL IESGHRICSL DSGNKHNNSH VDTVKSLPFL
560 570 580 590 600
SSSQQVLESN EFKPIGEAVI KVGAALQASG EAVFVDDIPT LPDCLHGAFI
610 620 630 640 650
YSTEPLAKIK SLSFRENVTP TGVFAVLTFK DIPQQGQNIG SKTLFGPGPL
660 670 680 690 700
FADELTRCAG QRIALVVADT QKHADMAAKL AVVEYDTKNL EQPILTVEDA
710 720 730 740 750
VKRSSFFEVH PMFYPEPVGD VIKGMEEAER KIISSELRLG SQYFFYMEPQ
760 770 780 790 800
TALALPDEDN CVKVFSSSQA PEYVHSVIAT CLGIQEHNVR VITRRVGGGF
810 820 830 840 850
GGKAVKSMPV ATACALGAYK LQRPVKMFLN RKTDMIMAGG RHPMKINYNV
860 870 880 890 900
GFRSDGKLTA LELTMLIDAG LEPDVSPIMP RNIMGPLRKY DWGALSFDVK
910 920 930 940 950
VCKTNCLSRT AMRAPGEVQG SYIAESIIEN VASSLQMDVD AVRKINLHTY
960 970 980 990 1000
DSLRKFYNHI AGDPDEYTLP LLWEKLEISS KFKERSEMVK EFNLCNVWRK
1010 1020 1030 1040 1050
RGISRVPIVH QVMQRPTPGK VSILSDGSVV VEVGGIEIGQ GLWTKVQQMV
1060 1070 1080 1090 1100
AYGLGMVKCE GNEKLLDRIR VVQSDTLGMI QGGFTAGSTT SESSCEAVRL
1110 1120 1130 1140 1150
CCVILVERLK PIMDQMMMEK SGSVTWNILI QQAYGQYINL SASTLYKPEY
1160 1170 1180 1190 1200
SSMEYLNYGV GVSEVEVDLV TGKTEILRSD IIYDCGKSLN PAVDLGQTEG
1210 1220 1230 1240 1250
AFVQGIGFFM MEEYTTDEKG LVVQQGTWDY KIPTVDTIPK HFNVEIVNTG
1260 1270 1280 1290 1300
HHKNRVLSSK ASGEPPLLLA ASVHCATRSA IREARKHSLS SNFIDGSDSE
1310 1320 1330
FELPVPATMP VVKSLCGLYS VEKYLQGKIK GQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8AZK7 | A0A1P8AZK7_ARATH | Abscisic aldehyde oxidase 3 | AAO3 ABSCISIC ALDEHYDE OXIDASE 3, abscisic aldehyde oxidase 3, Aldehyde oxidase delta, AOdelta, At-AO3 | 1,216 | Annotation score: | ||
A0A1P8AZL3 | A0A1P8AZL3_ARATH | Abscisic aldehyde oxidase 3 | AAO3 ABSCISIC ALDEHYDE OXIDASE 3, abscisic aldehyde oxidase 3, Aldehyde oxidase delta, AOdelta, At-AO3 | 1,060 | Annotation score: | ||
A0A1P8AZL6 | A0A1P8AZL6_ARATH | Abscisic aldehyde oxidase 3 | AAO3 ABSCISIC ALDEHYDE OXIDASE 3, abscisic aldehyde oxidase 3, Aldehyde oxidase delta, AOdelta, At-AO3 | 1,176 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB016622 mRNA Translation: BAA82672.1 AC007154 Genomic DNA Translation: AAD22498.1 CP002685 Genomic DNA Translation: AEC07944.1 CP002685 Genomic DNA Translation: AEC07945.1 AB010080 mRNA Translation: BAA28630.1 |
PIRi | D84669 T52176 |
RefSeqi | NP_001077966.1, NM_001084497.1 NP_180283.1, NM_128273.3 |
Genome annotation databases
EnsemblPlantsi | AT2G27150.1; AT2G27150.1; AT2G27150 AT2G27150.2; AT2G27150.2; AT2G27150 |
GeneIDi | 817257 |
Gramenei | AT2G27150.1; AT2G27150.1; AT2G27150 AT2G27150.2; AT2G27150.2; AT2G27150 |
KEGGi | ath:AT2G27150 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB016622 mRNA Translation: BAA82672.1 AC007154 Genomic DNA Translation: AAD22498.1 CP002685 Genomic DNA Translation: AEC07944.1 CP002685 Genomic DNA Translation: AEC07945.1 AB010080 mRNA Translation: BAA28630.1 |
PIRi | D84669 T52176 |
RefSeqi | NP_001077966.1, NM_001084497.1 NP_180283.1, NM_128273.3 |
3D structure databases
SMRi | Q7G9P4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT2G27150.2 |
PTM databases
iPTMneti | Q7G9P4 |
Proteomic databases
PaxDbi | Q7G9P4 |
PRIDEi | Q7G9P4 |
ProteomicsDBi | 244792 |
Genome annotation databases
EnsemblPlantsi | AT2G27150.1; AT2G27150.1; AT2G27150 AT2G27150.2; AT2G27150.2; AT2G27150 |
GeneIDi | 817257 |
Gramenei | AT2G27150.1; AT2G27150.1; AT2G27150 AT2G27150.2; AT2G27150.2; AT2G27150 |
KEGGi | ath:AT2G27150 |
Organism-specific databases
Araporti | AT2G27150 |
TAIRi | locus:2045149, AT2G27150 |
Phylogenomic databases
eggNOGi | KOG0430, Eukaryota |
HOGENOMi | CLU_001681_1_1_1 |
InParanoidi | Q7G9P4 |
OrthoDBi | 48717at2759 |
PhylomeDBi | Q7G9P4 |
Enzyme and pathway databases
BioCyci | ARA:AT2G27150-MONOMER MetaCyc:AT2G27150-MONOMER |
BRENDAi | 1.2.3.14, 399 |
SABIO-RKi | Q7G9P4 |
Miscellaneous databases
PROi | PR:Q7G9P4 |
Gene expression databases
ExpressionAtlasi | Q7G9P4, baseline and differential |
Genevisiblei | Q7G9P4, AT |
Family and domain databases
InterProi | View protein in InterPro IPR002888, 2Fe-2S-bd IPR036884, 2Fe-2S-bd_dom_sf IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR000674, Ald_Oxase/Xan_DH_a/b IPR036856, Ald_Oxase/Xan_DH_a/b_sf IPR016208, Ald_Oxase/xanthine_DH IPR008274, AldOxase/xan_DH_Mopterin-bd IPR037165, AldOxase/xan_DH_Mopterin-bd_sf IPR005107, CO_DH_flav_C IPR036683, CO_DH_flav_C_dom_sf IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR002346, Mopterin_DH_FAD-bd |
Pfami | View protein in Pfam PF01315, Ald_Xan_dh_C, 1 hit PF02738, Ald_Xan_dh_C2, 1 hit PF03450, CO_deh_flav_C, 1 hit PF00941, FAD_binding_5, 1 hit PF00111, Fer2, 1 hit PF01799, Fer2_2, 1 hit |
PIRSFi | PIRSF000127, Xanthine_DH, 1 hit |
SMARTi | View protein in SMART SM01008, Ald_Xan_dh_C, 1 hit SM01092, CO_deh_flav_C, 1 hit |
SUPFAMi | SSF47741, SSF47741, 1 hit SSF54292, SSF54292, 1 hit SSF54665, SSF54665, 1 hit SSF55447, SSF55447, 1 hit SSF56003, SSF56003, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit PS51387, FAD_PCMH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALDO3_ARATH | |
Accessioni | Q7G9P4Primary (citable) accession number: Q7G9P4 Secondary accession number(s): O64429 Q9SLZ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families