We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q7FAS1 (GLO3_ORYSJ)
Protein
Glycolate oxidase 3
Gene
GLO3
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Catalyzes the oxidation of glycolate to glyoxylate, with a reduction of O2 to H2O2. Is a key enzyme in photorespiration in green plants.
By similarityCatalytic activityi
Cofactori
FMNBy similarityNote: Binds 1 FMN per subunit.By similarity
: photorespiration Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes glycine from 2-phosphoglycolate.By similarity This subpathway is part of the pathway photorespiration, which is itself part of Photosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from 2-phosphoglycolate, the pathway photorespiration and in Photosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 25 | GlyoxylateBy similarity | 1 | |
Binding sitei | 107 | FMNBy similarity | 1 | |
Sitei | 109 | Involved in determining the substrate specificity of glycolate oxidaseBy similarity | 1 | |
Binding sitei | 130 | GlyoxylateBy similarity | 1 | |
Binding sitei | 156 | FMNBy similarity | 1 | |
Binding sitei | 165 | GlyoxylateBy similarity | 1 | |
Binding sitei | 231 | FMNBy similarity | 1 | |
Binding sitei | 253 | FMNBy similarity | 1 | |
Active sitei | 255 | Proton acceptorBy similarity | 1 | |
Binding sitei | 255 | GlyoxylateBy similarity | 1 | |
Binding sitei | 258 | GlyoxylateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 78 – 80 | FMNBy similarity | 3 | |
Nucleotide bindingi | 128 – 130 | FMNBy similarity | 3 | |
Nucleotide bindingi | 286 – 290 | FMNBy similarity | 5 | |
Nucleotide bindingi | 309 – 310 | FMNBy similarity | 2 |
GO - Molecular functioni
- (S)-2-hydroxy-acid oxidase activity Source: UniProtKB
- FMN binding Source: InterPro
GO - Biological processi
- cellular response to virus Source: UniProtKB
- oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
- photorespiration Source: UniProtKB
- regulation of photosynthesis Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Glycolate pathway, Photorespiration |
Ligand | Flavoprotein, FMN |
Enzyme and pathway databases
PlantReactomei | R-OSA-1119312, Photorespiration R-OSA-1119596, Glutamate biosynthesis I |
UniPathwayi | UPA00951;UER00912 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycolate oxidase 3 (EC:1.1.3.15By similarity)Short name: GOX 3 Short name: OsGLO3 Alternative name(s): Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 Short chain alpha-hydroxy acid oxidase GLO3 |
Gene namesi | Name:GLO3 Ordered Locus Names:Os04g0623500, LOC_Os04g53210 ORF Names:OsJ_16217, OSJNBa0053K19.8 |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Peroxisome
- peroxisome Source: UniProtKB
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000403413 | 1 – 367 | Glycolate oxidase 3Add BLAST | 367 |
Proteomic databases
PaxDbi | Q7FAS1 |
PRIDEi | Q7FAS1 |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 4530.OS04T0623500-02 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 360 | FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST | 360 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 365 – 367 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
HOGENOMi | CLU_020639_0_0_1 |
InParanoidi | Q7FAS1 |
OMAi | TFFQMYW |
OrthoDBi | 879633at2759 |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q7FAS1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELITNVSEY EQLAKQKLPK MIYDYYASGA EDQWTLKENR EAFSRILFRP
60 70 80 90 100
RILIDVSRIN MATNVLGFNI SMPIMIAPSA MQKMAHPEGE LATARAASAA
110 120 130 140 150
GTIMTLSSWS TSSVEEVNSA APGIRFFQLY VYKDRNIVRQ LVRRAELAGF
160 170 180 190 200
KAIALTVDTP RLGRREADIK NRFNLPPHLV LKNFEALDLG KMDKTNDSGL
210 220 230 240 250
ASYVASQVDR SLSWTDVKWL QTITSLPILV KGVMTAEDTR LAVESGAAGI
260 270 280 290 300
IVSNHGARQL DYVPATISCL EEVVREAKGR LPVFLDGGVR RGTDVFKALA
310 320 330 340 350
LGASGVFIGR PVLFSLAVDG EAGVRKVLQM LRDELELTMA LSGCTSLAEI
360
TRNHVITDSD RIRRSRL
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040390 | 306 – 307 | Missing in isoform 2. 1 Publication | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL606645 Genomic DNA Translation: CAE03500.2 AP008210 Genomic DNA Translation: BAF15839.1 AP014960 Genomic DNA Translation: BAS91083.1 AP014960 Genomic DNA Translation: BAS91084.1 CM000141 Genomic DNA Translation: EEE61716.1 AK060221 mRNA Translation: BAG87371.1 AK068638 mRNA Translation: BAG91005.1 |
RefSeqi | XP_015635502.1, XM_015780016.1 [Q7FAS1-1] XP_015635503.1, XM_015780017.1 [Q7FAS1-2] |
Genome annotation databases
EnsemblPlantsi | Os04t0623500-01; Os04t0623500-01; Os04g0623500 [Q7FAS1-2] Os04t0623500-02; Os04t0623500-02; Os04g0623500 [Q7FAS1-1] |
GeneIDi | 4337048 |
Gramenei | Os04t0623500-01; Os04t0623500-01; Os04g0623500 [Q7FAS1-2] Os04t0623500-02; Os04t0623500-02; Os04g0623500 [Q7FAS1-1] |
KEGGi | osa:4337048 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL606645 Genomic DNA Translation: CAE03500.2 AP008210 Genomic DNA Translation: BAF15839.1 AP014960 Genomic DNA Translation: BAS91083.1 AP014960 Genomic DNA Translation: BAS91084.1 CM000141 Genomic DNA Translation: EEE61716.1 AK060221 mRNA Translation: BAG87371.1 AK068638 mRNA Translation: BAG91005.1 |
RefSeqi | XP_015635502.1, XM_015780016.1 [Q7FAS1-1] XP_015635503.1, XM_015780017.1 [Q7FAS1-2] |
3D structure databases
SMRi | Q7FAS1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4530.OS04T0623500-02 |
Proteomic databases
PaxDbi | Q7FAS1 |
PRIDEi | Q7FAS1 |
Genome annotation databases
EnsemblPlantsi | Os04t0623500-01; Os04t0623500-01; Os04g0623500 [Q7FAS1-2] Os04t0623500-02; Os04t0623500-02; Os04g0623500 [Q7FAS1-1] |
GeneIDi | 4337048 |
Gramenei | Os04t0623500-01; Os04t0623500-01; Os04g0623500 [Q7FAS1-2] Os04t0623500-02; Os04t0623500-02; Os04g0623500 [Q7FAS1-1] |
KEGGi | osa:4337048 |
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
HOGENOMi | CLU_020639_0_0_1 |
InParanoidi | Q7FAS1 |
OMAi | TFFQMYW |
OrthoDBi | 879633at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00951;UER00912 |
PlantReactomei | R-OSA-1119312, Photorespiration R-OSA-1119596, Glutamate biosynthesis I |
Gene expression databases
Genevisiblei | Q7FAS1, OS |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GLO3_ORYSJ | |
Accessioni | Q7FAS1Primary (citable) accession number: Q7FAS1 Secondary accession number(s): A0A0P0WEZ5, B7E4S4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2011 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 23, 2022 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families