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Protein

Proteasome subunit beta

Gene

prcB

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The S.coelicolor proteasome is able to cleave oligopeptides after hydrophobic residues, but not after basic or acidic residues, thus displaying chymotrypsin-like activity but not trypsin-like activity.UniRule annotation1 Publication

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.UniRule annotation1 Publication

Activity regulationi

The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity (By similarity). Peptidolytic activity is completely inhibited by lactacystin, and to a lesser extent, by N-acetyl-Leu-Leu-norleucinal (Ac-LLnL) and benzoyloxycarbonyl-Leu-Leu-Leu-vinylsulfone (Z-LLL-VS) in vitro.UniRule annotation1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius.1 Publication

Pathwayi: proteasomal Pup-dependent pathway

This protein is involved in the pathway proteasomal Pup-dependent pathway, which is part of Protein degradation.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway proteasomal Pup-dependent pathway and in Protein degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei54NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

UniPathwayi
UPA00997

Protein family/group databases

MEROPSiT01.005

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit betaUniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome beta subunitUniRule annotation
Proteasome core protein PrcBUniRule annotation
Gene namesi
Name:prcBUniRule annotation
Ordered Locus Names:SCO1644
ORF Names:SCI41.27
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00003834891 – 53Removed in mature form; by autocatalysisUniRule annotation1 PublicationAdd BLAST53
ChainiPRO_500005507254 – 281Proteasome subunit betaAdd BLAST228

Keywords - PTMi

Autocatalytic cleavage, Zymogen

Proteomic databases

PRIDEiQ7AKQ5

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has probably a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is likely capped by the proteasome-associated ATPase, ARC.1 Publication

Protein-protein interaction databases

STRINGi100226.SCO1644

Structurei

3D structure databases

ProteinModelPortaliQ7AKQ5
SMRiQ7AKQ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KPK Bacteria
COG0638 LUCA
HOGENOMiHOG000245308
InParanoidiQ7AKQ5
KOiK03433
OMAiTMCAGVT
OrthoDBiPOG091H08LD
PhylomeDBiQ7AKQ5

Family and domain databases

Gene3Di3.60.20.10, 1 hit
HAMAPiMF_02113_B Proteasome_B_B, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR022483 Pept_T1A_Psome_suB_actinobac
IPR000243 Pept_T1A_subB
IPR001353 Proteasome_sua/b
IPR023333 Proteasome_suB-type
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PRINTSiPR00141 PROTEASOME
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR03690 20S_bact_beta, 1 hit
PROSITEiView protein in PROSITE
PS51476 PROTEASOME_BETA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7AKQ5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEANTRSTGR LPAAFLTPGS SSFMDFLGEH QPEMLPGNRQ LPPVQGVIEA
60 70 80 90 100
PHGTTIVAVT FPGGVVLAGD RRATMGNMIA QRDIEKVFPA DEYSAVGIAG
110 120 130 140 150
TAGLAVEMVK LFQLELEHFE KVEGAQLSLE GKANRLSTMI RSNLGMAMQG
160 170 180 190 200
LAVVPLFAGY DVDRGRGRIF SYDVTGGRSE ERHFATTGSG SVFARGAMKK
210 220 230 240 250
LFRDDLTEEQ ATTLVVQALY DAADDDSATG GPDVARRIYP IITVITEDGF
260 270 280
RRLGEDEAAE LAGSVLQARL EQPDGPRAAL L
Length:281
Mass (Da):30,086
Last modified:July 5, 2004 - v1
Checksum:i860B58A2B45CD8F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086832 Genomic DNA Translation: AAC64284.1
AL939109 Genomic DNA Translation: CAB59497.1
RefSeqiNP_625919.1, NC_003888.3
WP_003977182.1, NC_003888.3

Genome annotation databases

EnsemblBacteriaiCAB59497; CAB59497; CAB59497
GeneIDi1097075
KEGGisco:SCO1644
PATRICifig|100226.15.peg.1659

Similar proteinsi

Entry informationi

Entry nameiPSB_STRCO
AccessioniPrimary (citable) accession number: Q7AKQ5
Secondary accession number(s): O87598
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: September 12, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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