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Protein

Iron-regulated surface determinant protein A

Gene

isdA

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transfers its hemin to hemin-free IsdC (apo-IsdC) directly probably through the activated holo-IsdA-apo-IsdC complex and driven by the higher affinity of apo-IsdC for the cofactor. The reaction is reversible. Binds transferrin, lactoferrin, heme, hemoglobin, hemin, fetuin, asialofetuin and protein A. Also binds fibronectin and chains B-beta and gamma of fibrinogen. Could play a role in the removal of heme from hemoglobin. The IsdA-mediated iron-acquisition system from transferrin could play only an ancillary role in the iron uptake whereas the siderophore-mediated iron-acquisition system from transferrin seems to play an essential or dominant role. May function as a reservoir for heme. Involved in adherence of S.aureus to human desquamated nasal epithelial cells and is required for nasal colonization. Protects S.aureus against the bactericidal protease activity of apolactoferrin in vitro and confers resistance to bovine lactoferricin (By similarity). Also IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75HemeBy similarity1
Binding sitei82HemeBy similarity1
Metal bindingi166Iron (heme axial ligand)By similarity1

GO - Molecular functioni

Keywordsi

LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-regulated surface determinant protein A
Alternative name(s):
Fur-regulated protein A
Staphylococcal transferrin-binding protein A
Gene namesi
Name:isdA
Synonyms:frpA, stbA
Ordered Locus Names:MW1012
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000418 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Biotechnological usei

A combined vaccine containing IsdA, IsdB, SdrD and SdrE afforded significant protection in mice against a lethal challenge with S.aureus Newman or any of the clinical isolates NRS252, N315, NRS248, USA100 and USA400. The immune response elicited by the combined vaccine is greater than the one elicited by its individual components.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 46By similarityAdd BLAST46
ChainiPRO_000004609447 – 316Iron-regulated surface determinant protein AAdd BLAST270
PropeptideiPRO_0000046095317 – 350Removed by sortase APROSITE-ProRule annotationAdd BLAST34

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei316Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation1

Keywords - PTMi

Peptidoglycan-anchor

Expressioni

Inductioni

Repressed by fur in the presence of iron (By similarity). Transcriptionally up-regulated by hydrogen peroxide.By similarity1 Publication

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with IsdC.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ7A152
SMRiQ7A152
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 184NEATPROSITE-ProRule annotationAdd BLAST123

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi313 – 317LPXTG sorting signalPROSITE-ProRule annotation5

Domaini

The NEAT domain is responsible for binding Fe3+ and Fe2+ heme and fibrinogen. The NEAT domain is an inhibitor of apolactoferrin activity, while the C-domain confers resistance to bovine lactoferricin (By similarity).By similarity

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000107381
KOiK14193
OMAiYYFQAVL

Family and domain databases

CDDicd06920 NEAT, 1 hit
InterProiView protein in InterPro
IPR019948 Gram-positive_anchor
IPR006635 NEAT_dom
IPR037250 NEAT_dom_sf
PfamiView protein in Pfam
PF00746 Gram_pos_anchor, 1 hit
PF05031 NEAT, 1 hit
SMARTiView protein in SMART
SM00725 NEAT, 1 hit
SUPFAMiSSF158911 SSF158911, 1 hit
PROSITEiView protein in PROSITE
PS50847 GRAM_POS_ANCHORING, 1 hit
PS50978 NEAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7A152-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTKHYLNSKY QSEQRSSAMK KITMGTASII LGSLVYIGAD SQQVNAATEA
60 70 80 90 100
TNATNNQSTQ VSQATSQPIN FQVQKDGSSE KSHMDDYMQH PGKVIKQNNK
110 120 130 140 150
YYFQTVLNNA SFWKEYKFYN ANNQELATTV VNDNKKADTR TINVAVEPGY
160 170 180 190 200
KSLTTKVHIV VPQINYNHRY TTHLEFEKAI PTLADAAKPN NVKPVQPKPA
210 220 230 240 250
QPKTPTEQTK PVQPKVEKVK PTVTTTSKVE DNHSTKVVST DTTKDQTKTQ
260 270 280 290 300
TAHTVKTAQT AQEQNKVQTP VKDVATAKSE SNNQAVSDNK SQQTNKVTKH
310 320 330 340 350
NETPKQASKA KELPKTGLTS VDNFISTVAF ATLALLGSLS LLLFKRKESK
Length:350
Mass (Da):38,746
Last modified:July 5, 2004 - v1
Checksum:i14882D25C0EA3CA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA Translation: BAB94877.1
RefSeqiWP_000160859.1, NC_003923.1

Genome annotation databases

EnsemblBacteriaiBAB94877; BAB94877; BAB94877
KEGGisam:MW1012

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA Translation: BAB94877.1
RefSeqiWP_000160859.1, NC_003923.1

3D structure databases

ProteinModelPortaliQ7A152
SMRiQ7A152
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB94877; BAB94877; BAB94877
KEGGisam:MW1012

Phylogenomic databases

HOGENOMiHOG000107381
KOiK14193
OMAiYYFQAVL

Miscellaneous databases

PROiPR:Q7A152

Family and domain databases

CDDicd06920 NEAT, 1 hit
InterProiView protein in InterPro
IPR019948 Gram-positive_anchor
IPR006635 NEAT_dom
IPR037250 NEAT_dom_sf
PfamiView protein in Pfam
PF00746 Gram_pos_anchor, 1 hit
PF05031 NEAT, 1 hit
SMARTiView protein in SMART
SM00725 NEAT, 1 hit
SUPFAMiSSF158911 SSF158911, 1 hit
PROSITEiView protein in PROSITE
PS50847 GRAM_POS_ANCHORING, 1 hit
PS50978 NEAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiISDA_STAAW
AccessioniPrimary (citable) accession number: Q7A152
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 5, 2004
Last modified: November 22, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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