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Protein

Circadian clock protein KaiA

Gene

kaiA

Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it enhances the phosphorylation status of KaiC. In contrast, the presence of KaiB in the complex decreases the phosphorylation status of KaiC, suggesting that KaiB acts by antagonizing the interaction between KaiA and KaiC. A KaiA dimer is sufficient to enhance KaiC hexamer phosphorylation.

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • circadian rhythm Source: UniProtKB
  • protein phosphorylation Source: InterPro

Keywordsi

Biological processBiological rhythms

Enzyme and pathway databases

BioCyciTELO197221:G1G3I-495-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Circadian clock protein KaiA
Gene namesi
Name:kaiA
Ordered Locus Names:tlr0481
OrganismiThermosynechococcus elongatus (strain BP-1)
Taxonomic identifieri197221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeThermosynechococcus
Proteomesi
  • UP000000440 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi204Y → F: Induces a change in the structure diue to the absence of the hydrogen bond between D-266 and Y-204. 1 Publication1
Mutagenesisi266D → N: No effect. 1 Publication1
Mutagenesisi270H → A: Induces a decrease in activity and ability to enhance KaiC phosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002178691 – 283Circadian clock protein KaiAAdd BLAST283

Interactioni

Subunit structurei

Homodimer. Component of the KaiABC complex, at least composed of a KaiC homohexamer, a KaiB dimer and two KaiA dimers. The KaiABC complex also interacts with SasA (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-29355N
IntActiQ79V62, 1 interactor
MINTiQ79V62
STRINGi197221.tlr0481

Structurei

Secondary structure

1283
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi151 – 162Combined sources12
Beta strandi166 – 171Combined sources6
Helixi174 – 181Combined sources8
Helixi187 – 203Combined sources17
Helixi211 – 225Combined sources15
Helixi229 – 246Combined sources18
Helixi251 – 259Combined sources9
Helixi260 – 276Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q6ANMR-A/B180-283[»]
1Q6BNMR-A/B180-283[»]
1SUYNMR-A/B180-283[»]
1SV1NMR-A/B180-283[»]
1V2ZX-ray1.80A174-283[»]
5JWRX-ray2.61E/F/G/H147-283[»]
ProteinModelPortaliQ79V62
SMRiQ79V62
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ79V62

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 163KaiA N-terminalPROSITE-ProRule annotationAdd BLAST161
Domaini173 – 281KaiA C-terminalPROSITE-ProRule annotationAdd BLAST109

Domaini

The KaiA domain mediates the interaction with KaiC, the homodimerization, and is responsible for the clock oscillation function.

Phylogenomic databases

eggNOGiENOG4108WXF Bacteria
ENOG410YS5X LUCA
HOGENOMiHOG000276784
KOiK08480
OMAiHMELMDE
OrthoDBiPOG091H10AH

Family and domain databases

Gene3Di1.10.1240.30, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR011648 Circadian_clock_KaiA
IPR020844 Circadian_clock_KaiA_N
IPR020856 Circadian_clock_protein_KaiA_C
IPR017944 KaiA/RbsU_helical_domain_sf
PfamiView protein in Pfam
PF07688 KaiA, 1 hit
SMARTiView protein in SMART
SM01247 KaiA, 1 hit
SUPFAMiSSF101215 SSF101215, 1 hit
SSF52172 SSF52172, 1 hit
PROSITEiView protein in PROSITE
PS51431 KAIA_C, 1 hit
PS51430 KAIA_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q79V62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQSTALTIC GLVYSPAIGQ ELVRLHTSDI DELVYFSSER EFCNYLEARR
60 70 80 90 100
NSIACLILEW GEGTPQIITY LHHSATLLPA ILIFPAAPAP PPAGPHYHIA
110 120 130 140 150
EVILTTDQLD QLNRQIEEAI TGFVKLCPGC AVPPHVLFRL PALKESSNVD
160 170 180 190 200
PQHRLSQKLK ERLGYLGVYY KRDTAFFFRR MSPADKRKLL DELRSIYRTI
210 220 230 240 250
VLEYFNTDAK VNERIDEFVS KAFFADISVS QVLEIHVELM DTFSKQLKLE
260 270 280
GRSEDILLDY RLTLIDVIAH LCEMYRRSIP REV
Length:283
Mass (Da):32,368
Last modified:July 5, 2004 - v1
Checksum:iD3B0DCBE8A580B4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071375 Genomic DNA Translation: BAB85983.1
BA000039 Genomic DNA Translation: BAC08033.1
RefSeqiNP_681271.1, NC_004113.1
WP_011056332.1, NC_004113.1

Genome annotation databases

EnsemblBacteriaiBAC08033; BAC08033; BAC08033
GeneIDi1012550
KEGGitel:tlr0481
PATRICifig|197221.4.peg.506

Similar proteinsi

Entry informationi

Entry nameiKAIA_THEEB
AccessioniPrimary (citable) accession number: Q79V62
Secondary accession number(s): Q8RR35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: February 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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