UniProtKB - Q79PF6 (KAIA_SYNE7)
Protein
Circadian clock protein KaiA
Gene
kaiA
Organism
Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans R2)
Status
Functioni
Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it enhances the phosphorylation status of KaiC. In contrast, the presence of KaiB in the complex decreases the phosphorylation status of KaiC, suggesting that KaiB acts by antagonizing the interaction between KaiA and KaiC. A KaiA dimer is sufficient to enhance KaiC hexamer phosphorylation.4 Publications
Miscellaneous
'Kai' means 'cycle' in Japanese.
Caution
PubMed:15007067 structure is derived from this sequence and not from the one of S.elongatus.Curated
GO - Molecular functioni
- identical protein binding Source: IntAct
GO - Biological processi
- circadian rhythm Source: InterPro
- entrainment of circadian clock Source: UniProtKB
- negative regulation of protein dephosphorylation Source: CACAO
- positive regulation of circadian rhythm Source: UniProtKB
- protein phosphorylation Source: InterPro
Keywordsi
Biological process | Biological rhythms |
Enzyme and pathway databases
BioCyci | SYNEL:SYNPCC7942_1218-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Circadian clock protein KaiA |
Gene namesi | Name:kaiA Ordered Locus Names:Synpcc7942_1218 ORF Names:see0009 |
Organismi | Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans R2) |
Taxonomic identifieri | 1140 [NCBI] |
Taxonomic lineagei | Bacteria › Cyanobacteria › Synechococcales › Synechococcaceae › Synechococcus › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 9 | I → T: Extends the period of the circadian rhythm to 29 hours. 1 Publication | 1 | |
Mutagenesisi | 16 | I → F: Extends the period of the circadian rhythm to 27 hours. 1 Publication | 1 | |
Mutagenesisi | 31 | L → P: Extends the period of the circadian rhythm to 27 hours. 1 Publication | 1 | |
Mutagenesisi | 36 | S → P: Extends the period of the circadian rhythm to 29 hours. 1 Publication | 1 | |
Mutagenesisi | 53 | C → S: Induces an arrhythmic phenotype. 1 Publication | 1 | |
Mutagenesisi | 76 | V → G: Extends the period of the circadian rhythm to 28 hours. 1 Publication | 1 | |
Mutagenesisi | 103 | E → K in kaiA1; extends the period of the circadian rhythm to 33 hours and increases the interaction with KaiB. | 1 | |
Mutagenesisi | 113 | Q → R: Extends the period of the circadian rhythm to 33 hours. 1 Publication | 1 | |
Mutagenesisi | 117 | Q → L: Extends the period of the circadian rhythm to 26 hours. 1 Publication | 1 | |
Mutagenesisi | 119 | D → E: Extends the period of the circadian rhythm to 30 hours. 1 Publication | 1 | |
Mutagenesisi | 119 | D → G: Extends the period of the circadian rhythm to 26 hours. 1 Publication | 1 | |
Mutagenesisi | 131 | V → A: Extends the period of the circadian rhythm to 28 hours. 1 Publication | 1 | |
Mutagenesisi | 136 | D → V: Extends the period of the circadian rhythm to 30 hours. 1 Publication | 1 | |
Mutagenesisi | 136 | D → Y: Extends the period of the circadian rhythm to 29 hours. 1 Publication | 1 | |
Mutagenesisi | 166 | Y → C in kaiA3; induces an arrhythmic phenotype and reduces the interaction with KaiC. | 1 | |
Mutagenesisi | 178 | F → S or I: Induces an arrhythmic phenotype. 1 Publication | 1 | |
Mutagenesisi | 180 | R → H: Extends the period of the circadian rhythm to 26 hours. 1 Publication | 1 | |
Mutagenesisi | 194 | M → T: Extends the period of the circadian rhythm to 26 hours. 1 Publication | 1 | |
Mutagenesisi | 224 | F → S: Induces an arrhythmic phenotype. 1 Publication | 1 | |
Mutagenesisi | 225 | F → S: Induces an arrhythmic phenotype. 1 Publication | 1 | |
Mutagenesisi | 239 | E → G: Extends the period of the circadian rhythm to 28 hours. 1 Publication | 1 | |
Mutagenesisi | 241 | M → T: Extends the period of the circadian rhythm to 38 hours. 1 Publication | 1 | |
Mutagenesisi | 242 | D → V or G: Extends the period of the circadian rhythm to 27 hours. 1 Publication | 1 | |
Mutagenesisi | 243 | E → A: Extends the period of the circadian rhythm to 34 hours. 1 Publication | 1 | |
Mutagenesisi | 244 | F → V: Extends the period of the circadian rhythm to 26 hours. 1 Publication | 1 | |
Mutagenesisi | 245 | A → D: Extends the period of the circadian rhythm to 26 hours. 1 Publication | 1 | |
Mutagenesisi | 249 | R → H in kaiA1; extends the period of the circadian rhythm to 30 hours. | 1 | |
Mutagenesisi | 266 | I → V: Extends the period of the circadian rhythm to 27 hours. 1 Publication | 1 | |
Mutagenesisi | 273 | C → Y: Extends the period of the circadian rhythm to 30 hours. 1 Publication | 1 | |
Mutagenesisi | 274 | E → K in kaiA3; induces an arrhythmic phenotype. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000217871 | 1 – 284 | Circadian clock protein KaiAAdd BLAST | 284 |
Proteomic databases
PRIDEi | Q79PF6 |
Expressioni
Developmental stagei
Expressed constantly throughout the circadian cycle.1 Publication
Interactioni
Subunit structurei
Homodimer.
Component of the KaiABC complex, at least composed of a KaiC homohexamer, a KaiB dimer and two KaiA dimers. The KaiABC complex also interacts with SasA.
2 PublicationsBinary interactionsi
Hide detailsQ79PF6
With | #Exp. | IntAct |
---|---|---|
itself | 5 | EBI-592281,EBI-592281 |
kaiB [Q79PF5] | 11 | EBI-592281,EBI-619150 |
kaiC [Q79PF4] | 22 | EBI-592281,EBI-592287 |
ldpA [Q8GLI4] | 2 | EBI-592281,EBI-626836 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
DIPi | DIP-33329N |
IntActi | Q79PF6, 4 interactors |
STRINGi | 1140.Synpcc7942_1218 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q79PF6 |
SMRi | Q79PF6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q79PF6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 164 | KaiA N-terminalPROSITE-ProRule annotationAdd BLAST | 164 | |
Domaini | 174 – 282 | KaiA C-terminalPROSITE-ProRule annotationAdd BLAST | 109 |
Domaini
The N-terminal part (1-102), which is also called pseudo-receiver domain, does not enhance KaiC autophosphorylation. It may have an amplitude-amplifier function and may be involved in transducing input signals to the KaiABC complex. This domain is not conserved in other species.1 Publication
The KaiA domain mediates the interaction with KaiC, the homodimerization, and is responsible for the clock oscillation function.1 Publication
Phylogenomic databases
eggNOGi | ENOG502Z8HQ, Bacteria |
HOGENOMi | CLU_911234_0_0_3 |
OMAi | VMAHLCE |
OrthoDBi | 1326064at2 |
Family and domain databases
Gene3Di | 1.10.1240.30, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR011648, Circadian_clock_KaiA IPR020844, Circadian_clock_KaiA_N IPR020856, Circadian_clock_protein_KaiA_C IPR017944, KaiA/RbsU_helical_domain_sf |
Pfami | View protein in Pfam PF07688, KaiA, 1 hit |
SMARTi | View protein in SMART SM01247, KaiA, 1 hit |
SUPFAMi | SSF101215, SSF101215, 1 hit SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS51431, KAIA_C, 1 hit PS51430, KAIA_N, 1 hit |
i Sequence
Sequence statusi: Complete.
Q79PF6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSQIAICIW VESTAILQDC QRALSADRYQ LQVCESGEML LEYAQTHRDQ
60 70 80 90 100
IDCLILVAAN PSFRAVVQQL CFEGVVVPAI VVGDRDSEDP DEPAKEQLYH
110 120 130 140 150
SAELHLGIHQ LEQLPYQVDA ALAEFLRLAP VETMADHIML MGANHDPELS
160 170 180 190 200
SQQRDLAQRL QERLGYLGVY YKRDPDRFLR NLPAYESQKL HQAMQTSYRE
210 220 230 240 250
IVLSYFSPNS NLNQSIDNFV NMAFFADVPV TKVVEIHMEL MDEFAKKLRV
260 270 280
EGRSEDILLD YRLTLIDVIA HLCEMYRRSI PRET
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB010691 Genomic DNA Translation: BAA37101.1 AY120853 Genomic DNA Translation: AAM82684.1 CP000100 Genomic DNA Translation: ABB57248.1 |
PIRi | T44267 |
RefSeqi | WP_011377921.1, NC_007604.1 |
Genome annotation databases
EnsemblBacteriai | ABB57248; ABB57248; Synpcc7942_1218 |
KEGGi | syf:Synpcc7942_1218 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB010691 Genomic DNA Translation: BAA37101.1 AY120853 Genomic DNA Translation: AAM82684.1 CP000100 Genomic DNA Translation: ABB57248.1 |
PIRi | T44267 |
RefSeqi | WP_011377921.1, NC_007604.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1M2E | NMR | - | A | 1-135 | [»] | |
1M2F | NMR | - | A | 1-135 | [»] | |
1R8J | X-ray | 2.03 | A/B | 2-284 | [»] | |
4G86 | X-ray | 2.39 | A/B | 2-284 | [»] | |
5C5E | X-ray | 2.82 | A/B | 1-284 | [»] | |
5N8Y | electron microscopy | 4.70 | M/N/O/P/Q/R/S/T/U/V/W/X | 1-284 | [»] | |
BMRBi | Q79PF6 | |||||
SMRi | Q79PF6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-33329N |
IntActi | Q79PF6, 4 interactors |
STRINGi | 1140.Synpcc7942_1218 |
Proteomic databases
PRIDEi | Q79PF6 |
Genome annotation databases
EnsemblBacteriai | ABB57248; ABB57248; Synpcc7942_1218 |
KEGGi | syf:Synpcc7942_1218 |
Phylogenomic databases
eggNOGi | ENOG502Z8HQ, Bacteria |
HOGENOMi | CLU_911234_0_0_3 |
OMAi | VMAHLCE |
OrthoDBi | 1326064at2 |
Enzyme and pathway databases
BioCyci | SYNEL:SYNPCC7942_1218-MONOMER |
Miscellaneous databases
EvolutionaryTracei | Q79PF6 |
Family and domain databases
Gene3Di | 1.10.1240.30, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR011648, Circadian_clock_KaiA IPR020844, Circadian_clock_KaiA_N IPR020856, Circadian_clock_protein_KaiA_C IPR017944, KaiA/RbsU_helical_domain_sf |
Pfami | View protein in Pfam PF07688, KaiA, 1 hit |
SMARTi | View protein in SMART SM01247, KaiA, 1 hit |
SUPFAMi | SSF101215, SSF101215, 1 hit SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS51431, KAIA_C, 1 hit PS51430, KAIA_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KAIA_SYNE7 | |
Accessioni | Q79PF6Primary (citable) accession number: Q79PF6 Secondary accession number(s): Q31NX1, Q9Z3H4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | July 5, 2004 | |
Last modified: | April 7, 2021 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references