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Protein

Circadian clock protein kinase KaiC

Gene

kaiC

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction.2 Publications

Miscellaneous

'Kai' means 'cycle' in Japanese.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+1 Publication

Activity regulationi

The interaction with KaiA enhances its phosphorylation status, while the interaction with KaiB decreases it. A KaiA dimer is sufficient to enhance KaiC phosphorylation.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi295Magnesium1
Metal bindingi318Magnesium1
Metal bindingi319Magnesium1
Metal bindingi378Magnesium1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 53ATP8
Nucleotide bindingi288 – 295ATP8

GO - Molecular functioni

GO - Biological processi

  • circadian rhythm Source: UniProtKB-UniRule
  • entrainment of circadian clock Source: UniProtKB
  • negative regulation of circadian rhythm Source: CACAO
  • regulation of phosphorelay signal transduction system Source: CACAO
  • regulation of transcription, DNA-templated Source: InterPro

Keywordsi

Molecular functionDNA-binding, Kinase, Repressor, Serine/threonine-protein kinase, Transferase
Biological processBiological rhythms, Transcription, Transcription regulation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_1216-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Circadian clock protein kinase KaiC (EC:2.7.11.1)
Gene namesi
Name:kaiC
Ordered Locus Names:Synpcc7942_1216
ORF Names:see0011
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000002717 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52K → A: Induces an arrhythmic phenotype. 1 Publication1
Mutagenesisi71G → A: Lowers the amplitude and distords the waveform of the circadian rhythm. 1 Publication1
Mutagenesisi87A → V in kaiC1; shortens the period of the circadian rhythm to 22 hours. 1
Mutagenesisi114G → A: Extends the period of the circadian rhythm to 27 hours. 1 Publication1
Mutagenesisi115Q → A: Abolishes the circadian rhythm. 1 Publication1
Mutagenesisi157S → C in kaiC2; extends the period of the circadian rhythm to 29 hours. 1
Mutagenesisi215R → C in kaiC3; shortens the period of the circadian rhythm to 16 hours and decreases the interaction with KaiA. 1
Mutagenesisi236P → S in kaiC4; extends the period of the circadian rhythm to 28 hours. 1
Mutagenesisi248P → L in kaiC6; induces a low amplitude phenotype and decreases the interaction with KaiA. 1
Mutagenesisi248P → S in kaiC5; induces an arrhythmic phenotype. 1
Mutagenesisi253R → H in kaiC7; extends the period of the circadian rhythm to 40 hours. 1
Mutagenesisi273M → I in kaiC8; extends the period of the circadian rhythm to 37 hours. 1
Mutagenesisi294K → A: Induces a long period phenotype. 1 Publication1
Mutagenesisi321R → Q in kaiC9; shortens the period of the circadian rhythm to 21 hours. 1
Mutagenesisi409T → A in kaiC10; extends the period of the circadian rhythm to 27 hours. 1
Mutagenesisi421G → R in kaiC11; extends the period of the circadian rhythm to 44 hours and increases the interaction with KaiA. 1
Mutagenesisi422A → T in kaiC15; able to suppress the kaiA2 extended phenotype. 1
Mutagenesisi432T → A: Loss of function. 1 Publication1
Mutagenesisi442Y → H in kaiC12; extends the period of the circadian rhythm to 60 hours and increases the interaction with KaiA. 1
Mutagenesisi460G → E in kaiC13; induces an arrhythmic phenotype. 1
Mutagenesisi495T → A in kaiC14; induces an arrhythmic phenotype. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002177821 – 519Circadian clock protein kinase KaiCAdd BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei432Phosphothreonine; by autocatalysis1 Publication1

Post-translational modificationi

Phosphorylated on serine/threonine residues by autocatalysis. Both phosphorylated and unphosphorylated forms exist. Can probably autophosphorylate and autodephosphorylate. Phosphorylated form correlates with clock speed.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ79PF4

PTM databases

iPTMnetiQ79PF4

Expressioni

Developmental stagei

Accumulates in a circadian fashion, peaking at circadian time (CT) 15-18.1 Publication

Inductioni

Down-regulated by itself.1 Publication

Interactioni

Subunit structurei

Homohexamer; hexamerization is dependent on ATP-binding. Core component of the KaiABC complex, at least composed of a KaiC homohexamer, a KaiB dimer and two KaiA dimers. Interacts directly with SasA.5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-33330N
IntActiQ79PF4, 3 interactors
MINTiQ79PF4
STRINGi1140.Synpcc7942_1216

Structurei

Secondary structure

1519
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ79PF4
SMRiQ79PF4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ79PF4

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 260KaiC 1Add BLAST242
Domaini261 – 493KaiC 2Add BLAST233

Domaini

The KaiC domains mediate the interaction with KaiA.

Sequence similaritiesi

Belongs to the KaiC family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105EHU Bacteria
COG0467 LUCA
HOGENOMiHOG000230416
KOiK08482
OrthoDBiPOG091H0SAA

Family and domain databases

HAMAPiMF_01836 KaiC, 1 hit
InterProiView protein in InterPro
IPR013503 Circadian_KaiC_bact
IPR030665 KaiC
IPR014774 KaiC-like_dom
IPR010624 KaiC_dom
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF06745 ATPase, 2 hits
PIRSFiPIRSF039117 KaiC, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR02655 circ_KaiC, 1 hit
PROSITEiView protein in PROSITE
PS51146 KAIC, 2 hits

Sequencei

Sequence statusi: Complete.

Q79PF4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSAEMTSPN NNSEHQAIAK MRTMIEGFDD ISHGGLPIGR STLVSGTSGT
60 70 80 90 100
GKTLFSIQFL YNGIIEFDEP GVFVTFEETP QDIIKNARSF GWDLAKLVDE
110 120 130 140 150
GKLFILDASP DPEGQEVVGG FDLSALIERI NYAIQKYRAR RVSIDSVTSV
160 170 180 190 200
FQQYDASSVV RRELFRLVAR LKQIGATTVM TTERIEEYGP IARYGVEEFV
210 220 230 240 250
SDNVVILRNV LEGERRRRTL EILKLRGTSH MKGEYPFTIT DHGINIFPLG
260 270 280 290 300
AMRLTQRSSN VRVSSGVVRL DEMCGGGFFK DSIILATGAT GTGKTLLVSR
310 320 330 340 350
FVENACANKE RAILFAYEES RAQLLRNAYS WGMDFEEMER QNLLKIVCAY
360 370 380 390 400
PESAGLEDHL QIIKSEINDF KPARIAIDSL SALARGVSNN AFRQFVIGVT
410 420 430 440 450
GYAKQEEITG LFTNTSDQFM GAHSITDSHI STITDTIILL QYVEIRGEMS
460 470 480 490 500
RAINVFKMRG SWHDKAIREF MISDKGPDIK DSFRNFERII SGSPTRITVD
510
EKSELSRIVR GVQEKGPES
Length:519
Mass (Da):58,003
Last modified:July 5, 2004 - v1
Checksum:i855DFE131EFFF3CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010691 Genomic DNA Translation: BAA37103.1
AY120853 Genomic DNA Translation: AAM82686.1
CP000100 Genomic DNA Translation: ABB57246.1
PIRiT44269
RefSeqiWP_011242648.1, NC_007604.1

Genome annotation databases

EnsemblBacteriaiABB57246; ABB57246; Synpcc7942_1216
KEGGisyf:Synpcc7942_1216

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010691 Genomic DNA Translation: BAA37103.1
AY120853 Genomic DNA Translation: AAM82686.1
CP000100 Genomic DNA Translation: ABB57246.1
PIRiT44269
RefSeqiWP_011242648.1, NC_007604.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TF7X-ray2.80A/B/C/D/E/F1-519[»]
1U9IX-ray2.80A/B/C/D/E/F1-519[»]
2GBLX-ray2.80A/B/C/D/E/F1-519[»]
3DVLX-ray2.80A/B/C/D/E/F1-519[»]
3JZMX-ray2.90A/B/C/D/E/F1-519[»]
3K09X-ray3.20A/B/C/D/E/F1-519[»]
3K0AX-ray3.00A/B/C/D/E/F1-519[»]
3K0CX-ray3.30A/B/C/D/E/F1-519[»]
3K0EX-ray3.20A/B/C/D/E/F1-519[»]
3K0FX-ray3.00A/B/C/D/E/F1-519[»]
3S1AX-ray3.00A/B/C/D/E/F1-519[»]
4DUGX-ray3.30A/B/C/D/E/F1-519[»]
4IJMX-ray3.35A/B/C/D/E/F14-519[»]
4TL6X-ray1.76A/B/C1-253[»]
4TL7X-ray1.94A/B/C/D/E/F1-253[»]
4TL8X-ray1.86A/B/C/D/E/F1-253[»]
4TL9X-ray1.82A/B/C/D/E/F1-253[»]
4TLAX-ray1.80A/B/C/D/E/F1-253[»]
4TLBX-ray1.98A/B/C/D/E/F1-253[»]
4TLCX-ray2.09A/B/C/D/E/F1-253[»]
4TLDX-ray1.95A/B/C/D/E/F1-253[»]
4TLEX-ray1.94A/B/C/D/E/F1-253[»]
5C5EX-ray2.82G/H500-519[»]
5N8Yelectron microscopy4.70A/B/C/D/E/F1-519[»]
ProteinModelPortaliQ79PF4
SMRiQ79PF4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-33330N
IntActiQ79PF4, 3 interactors
MINTiQ79PF4
STRINGi1140.Synpcc7942_1216

PTM databases

iPTMnetiQ79PF4

Proteomic databases

PRIDEiQ79PF4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB57246; ABB57246; Synpcc7942_1216
KEGGisyf:Synpcc7942_1216

Phylogenomic databases

eggNOGiENOG4105EHU Bacteria
COG0467 LUCA
HOGENOMiHOG000230416
KOiK08482
OrthoDBiPOG091H0SAA

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_1216-MONOMER

Miscellaneous databases

EvolutionaryTraceiQ79PF4

Family and domain databases

HAMAPiMF_01836 KaiC, 1 hit
InterProiView protein in InterPro
IPR013503 Circadian_KaiC_bact
IPR030665 KaiC
IPR014774 KaiC-like_dom
IPR010624 KaiC_dom
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF06745 ATPase, 2 hits
PIRSFiPIRSF039117 KaiC, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR02655 circ_KaiC, 1 hit
PROSITEiView protein in PROSITE
PS51146 KAIC, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiKAIC_SYNE7
AccessioniPrimary (citable) accession number: Q79PF4
Secondary accession number(s): Q31NX3, Q9Z3H2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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