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Protein

Transmembrane emp24 domain-containing protein 3

Gene

Tmed3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network (By similarity).By similarity

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: GO_Central
  • Golgi organization Source: GO_Central
  • intracellular protein transport Source: GO_Central

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 3
Alternative name(s):
p24 family protein gamma-4
Short name:
p24gamma4
Gene namesi
Name:Tmed3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1913361 Tmed3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 184LumenalSequence analysisAdd BLAST157
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
Topological domaini206 – 221CytoplasmicSequence analysisAdd BLAST16

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000001038526 – 221Transmembrane emp24 domain-containing protein 3Add BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei103Dimethylated arginine; alternateBy similarity1
Modified residuei103Omega-N-methylated arginine; alternateBy similarity1

Keywords - PTMi

Methylation

Proteomic databases

EPDiQ78IS1
MaxQBiQ78IS1
PaxDbiQ78IS1
PeptideAtlasiQ78IS1
PRIDEiQ78IS1

PTM databases

PhosphoSitePlusiQ78IS1

Expressioni

Gene expression databases

BgeeiENSMUSG00000032353 Expressed in 306 organ(s), highest expression level in membranous labyrinth
CleanExiMM_TMED3
GenevisibleiQ78IS1 MM

Interactioni

Subunit structurei

Monomer in endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED3; however, there are conflicting reports on the interaction. Interacts with GORASP1 and GORASP2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058723

Structurei

3D structure databases

ProteinModelPortaliQ78IS1
SMRiQ78IS1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 124GOLDPROSITE-ProRule annotationAdd BLAST83

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi208 – 221COPI vesicle coat-bindingSequence analysisAdd BLAST14
Motifi208 – 209COPII vesicle coat-bindingSequence analysis2

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1693 Eukaryota
ENOG4111JPD LUCA
GeneTreeiENSGT00670000097656
HOGENOMiHOG000160229
HOVERGENiHBG000271
InParanoidiQ78IS1
KOiK20350
OMAiNYDVDCF
OrthoDBiEOG091G0LX8
PhylomeDBiQ78IS1
TreeFamiTF313000

Family and domain databases

InterProiView protein in InterPro
IPR009038 GOLD_dom
IPR036598 GOLD_dom_sf
IPR015720 TMP21-related
PANTHERiPTHR22811 PTHR22811, 1 hit
PfamiView protein in Pfam
PF01105 EMP24_GP25L, 1 hit
SMARTiView protein in SMART
SM01190 EMP24_GP25L, 1 hit
SUPFAMiSSF101576 SSF101576, 1 hit
PROSITEiView protein in PROSITE
PS50866 GOLD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q78IS1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVHEAPHASS FQMLLQLLLL LLLRAEPLRS AELTFELPDN AKQCFHEEVE
60 70 80 90 100
QGVKFSLDYQ VITGGHYDVD CYVEDPRGNV IYRETKKQYD SFTYKTEAKG
110 120 130 140 150
VYRFCFSNEF STFSHKTVYF DFQVGDEPPI LPDMGNRVTA LTQMESACVT
160 170 180 190 200
IHEALKTVID SQTHYRLREA QDRARAEDLN SRVSYWSVGE TIALFVVSFS
210 220
QVLLLKSFFT EKRPVNRAVH S
Length:221
Mass (Da):25,465
Last modified:July 5, 2004 - v1
Checksum:iA1DA79631DAFB8DC
GO

Sequence cautioni

The sequence BAB22292 differs from that shown. Reason: Frameshift at positions 13 and 30.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18L → A in BAB23361 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002698 mRNA Translation: BAB22292.1 Frameshift.
AK004545 mRNA Translation: BAB23361.1
BC023338 mRNA Translation: AAH23338.1
CCDSiCCDS23396.1
RefSeqiNP_079636.2, NM_025360.2
UniGeneiMm.27606

Genome annotation databases

EnsembliENSMUST00000058488; ENSMUSP00000058723; ENSMUSG00000032353
GeneIDi66111
KEGGimmu:66111
UCSCiuc009qzo.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002698 mRNA Translation: BAB22292.1 Frameshift.
AK004545 mRNA Translation: BAB23361.1
BC023338 mRNA Translation: AAH23338.1
CCDSiCCDS23396.1
RefSeqiNP_079636.2, NM_025360.2
UniGeneiMm.27606

3D structure databases

ProteinModelPortaliQ78IS1
SMRiQ78IS1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058723

PTM databases

PhosphoSitePlusiQ78IS1

Proteomic databases

EPDiQ78IS1
MaxQBiQ78IS1
PaxDbiQ78IS1
PeptideAtlasiQ78IS1
PRIDEiQ78IS1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058488; ENSMUSP00000058723; ENSMUSG00000032353
GeneIDi66111
KEGGimmu:66111
UCSCiuc009qzo.2 mouse

Organism-specific databases

CTDi23423
MGIiMGI:1913361 Tmed3

Phylogenomic databases

eggNOGiKOG1693 Eukaryota
ENOG4111JPD LUCA
GeneTreeiENSGT00670000097656
HOGENOMiHOG000160229
HOVERGENiHBG000271
InParanoidiQ78IS1
KOiK20350
OMAiNYDVDCF
OrthoDBiEOG091G0LX8
PhylomeDBiQ78IS1
TreeFamiTF313000

Enzyme and pathway databases

ReactomeiR-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Miscellaneous databases

PROiPR:Q78IS1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032353 Expressed in 306 organ(s), highest expression level in membranous labyrinth
CleanExiMM_TMED3
GenevisibleiQ78IS1 MM

Family and domain databases

InterProiView protein in InterPro
IPR009038 GOLD_dom
IPR036598 GOLD_dom_sf
IPR015720 TMP21-related
PANTHERiPTHR22811 PTHR22811, 1 hit
PfamiView protein in Pfam
PF01105 EMP24_GP25L, 1 hit
SMARTiView protein in SMART
SM01190 EMP24_GP25L, 1 hit
SUPFAMiSSF101576 SSF101576, 1 hit
PROSITEiView protein in PROSITE
PS50866 GOLD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTMED3_MOUSE
AccessioniPrimary (citable) accession number: Q78IS1
Secondary accession number(s): Q9DC62, Q9DCK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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