Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 78 (07 Oct 2020)
Sequence version 1 (05 Jul 2004)
Previous versions | rss
Help videoAdd a publicationFeedback
Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Hirame rhabdovirus (strain Korea/CA 9703/1997) (HIRRV)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.By similarity
RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, Multifunctional enzyme, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological processmRNA capping, mRNA processing, Viral RNA replication
LigandATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 4 domains:
RNA-directed RNA polymerase (EC:2.7.7.48By similarity)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56By similarity)
GDP polyribonucleotidyltransferase (EC:2.7.7.88By similarity)
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 (EC:2.1.1.296By similarity)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:L
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHirame rhabdovirus (strain Korea/CA 9703/1997) (HIRRV)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri453457 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaOrthornaviraeNegarnaviricotaHaploviricotinaMonjiviricetesMononegaviralesRhabdoviridaeNovirhabdovirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiAcanthopagrus schlegelii (Black porgy) [TaxID: 72011]
Paralichthys olivaceus (Bastard halibut) (Hippoglossus olivaceus) [TaxID: 8255]
Plecoglossus altivelis (Ayu) [TaxID: 61084]
Sebastes inermis (Dark-banded rockfish) [TaxID: 160818]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000008118 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Virion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002978361 – 1986RNA-directed RNA polymerase LAdd BLAST1986

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q77SJ8

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

May form homodimer.

Interacts with the P protein.

By similarity

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini560 – 757RdRp catalyticPROSITE-ProRule annotationAdd BLAST198
Domaini1592 – 1788Mononegavirus-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd BLAST197

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the rhabdoviridae protein L family.Curated

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR026890, Mononeg_mRNAcap
IPR014023, Mononeg_RNA_pol_cat
IPR025786, Mononega_L_MeTrfase

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF14318, Mononeg_mRNAcap, 1 hit
PF00946, Mononeg_RNA_pol, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50526, RDRP_SSRNA_NEG_NONSEG, 1 hit
PS51590, SAM_MT_MNV_L, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q77SJ8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDFFDLDIEI KQERLPAECS LNSPLNISLS SQLTDRMTPQ NENIRRQRER
60 70 80 90 100
IRTHTKTHSR IKHLSKLDND STRLHARLTE DLIKLQHLEV DSPVFDNWAL
110 120 130 140 150
LTSYYAALDY TLPERASFDW GQAAPYWNLY TQLRTILLQS QKIRKKDRGV
160 170 180 190 200
REIYSCGPLR LEFVEGTVLY FTDKQSGGEF TKSGELPSIT PYADFLAWVK
210 220 230 240 250
IISQRAQAVL MAVILRVTDK GLSPLPESLL SVYQTVDDIL KRAGQPAIDL
260 270 280 290 300
LKLWEPLVIT KLGELLGDRF GLEEDFRLTI RGEATRLAKK LAITNGLNRL
310 320 330 340 350
MTVLDSQTEA QPLFQFFGLF KHFAYPRVFS RDTIQAIQEV SDRPSSISAA
360 370 380 390 400
EFLHDQCEIR KEFYIRYLKA YHRAPGLDLS ALSPSSFLRE SLEHGKIPNE
410 420 430 440 450
KSPHYSNKEW YFIKFTKSIE WPISDTLSTF LSDKAITRDR AAWIEEGHSG
460 470 480 490 500
RDMSEKRLLL KFIKENFSSV AEIVAAADAI YNNEGDRLIA LKVKEMELKI
510 520 530 540 550
KGRGFGLMTF MPRLLQVLRE SIAKKTSKLF PEITMTSSDL DMKKRKFMLS
560 570 580 590 600
KRSDDRRGFI HINKSLDINK FCTSQRQFNS SAVFSSLDEM MGTFPLFSRV
610 620 630 640 650
HEIFEKTWIV DGSASDPPDL SHFTRILEEC RLHGIEAPHV WADGVFSGLK
660 670 680 690 700
GGIEGLCQYV WTICLLLRVE RVMQKTKLTH YILAQGDNVI ITIIVPVEIH
710 720 730 740 750
RDGIISDQES RRLLTLSRNI DLSLESELEK SGLTLKIEET LTSENISIYG
760 770 780 790 800
KDLHCPQHLT LALKKAASAA IISSEQYQDI PTFLSGLGTS LESLSECVNS
810 820 830 840 850
KTGAHLFGVL MGVAGWKDLA THQTWRGWRY PYHKAPLSGR VRASDMKIGK
860 870 880 890 900
GEAVELTIPV MSPRQQGKET LRELLANSLL GSALGMLAFP TPIDLEKRGV
910 920 930 940 950
GDYITHRLAI ARKALLSEKL DPHIEKRVRS ACNLPLSSRV DLSKLFDSPF
960 970 980 990 1000
SLNLATEEDA TAVIKRQAKK TLRLQEIGND KLRAQIGNMD KGIAALDADL
1010 1020 1030 1040 1050
AGAETINPRL NHMIRDITDE KESEMFVTKF ASARTMRTLA MGDSSEVPIV
1060 1070 1080 1090 1100
VLLEKKSQQK ELYTIWRARR PHATMWKCST VLAKGLRDIS WGKTIVGVTS
1110 1120 1130 1140 1150
PSPIEAMETT HIDPTDWEDS RSRETLSINY YLSRAGIDEQ TAKLTRGSLV
1160 1170 1180 1190 1200
PYYGTQTKPL IAKAYLELKG NPRTNKALLL LSVRESLVKT GSNLDELIMQ
1210 1220 1230 1240 1250
LCSHALDIDA ASLPALRAQE EATAGEGLRG GIKESMSPVG PDNFYTNITH
1260 1270 1280 1290 1300
KVFNRKWVTP YHVNIADFII QGLIETRKHL ILNEKMDGLL PLSSVKCTAC
1310 1320 1330 1340 1350
FRKKEREFFD IPEGPTWKND STTSDPAYTY FTTWCDLPRV STLPTMDQQS
1360 1370 1380 1390 1400
ATRLLGRGLS LNRPTAGEII TKFYSMSMES QRLLHPVDLL LGYGEGVVFG
1410 1420 1430 1440 1450
YIRSQHIHHG ALFQTKRETL TNKLRKFILD TKTQHAKQIG YLFQDEDSLH
1460 1470 1480 1490 1500
ELMAQGLCPY VPRSIPLTIT ELTNACAITT IRATEVILSA GSRVALMPVQ
1510 1520 1530 1540 1550
AIDETDVDNS RLAANTMQTI LGDSRPMNPV YLDCDLTTNM TAWESSIELD
1560 1570 1580 1590 1600
VLKSENFHID GLLMDLTARE LPISDTPWKQ RDWTCSNDPR IIAKGIKTKS
1610 1620 1630 1640 1650
LFIHQGVAEA LNMTPDLLVV IGGGLGGCAV PYLQEWADVP LIFATLFDER
1660 1670 1680 1690 1700
ERISEDGDLV VPPEILVRGM APRMIERELL EAELCDVTND GNRRLLTRLV
1710 1720 1730 1740 1750
KKNRGKGTVV LIDEIENRGA PESLLQSSLQ DLVRRLDKVC TLTSIHTVRE
1760 1770 1780 1790 1800
STVEQFAQRT NSIKRDRKTV TLHWNRYNRR DQFEALVIVK GEETRSDYHV
1810 1820 1830 1840 1850
STATAAQAFR KIDEQLEVEG RLSATRWSLP TLPAREKEIL FGYVSSVFLK
1860 1870 1880 1890 1900
TNLVLSADDM DRETLLETIE DTAPGLISWK EKLEHRDHAF RSDIDEKGIT
1910 1920 1930 1940 1950
QDKVFNLICL AWVITGLRYG IWETDAQSII TKTVYITRGP KLCPLGEKPK
1960 1970 1980
RVFASFKLQS DKRVEDAKGF LSALLHLEGF FPLGRQ
Length:1,986
Mass (Da):224,461
Last modified:July 5, 2004 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3A6254B6704D0C59
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF104985 Genomic RNA Translation: AAQ73462.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104985 Genomic RNA Translation: AAQ73462.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Proteomic databases

PRIDEiQ77SJ8

Family and domain databases

InterProiView protein in InterPro
IPR026890, Mononeg_mRNAcap
IPR014023, Mononeg_RNA_pol_cat
IPR025786, Mononega_L_MeTrfase
PfamiView protein in Pfam
PF14318, Mononeg_mRNAcap, 1 hit
PF00946, Mononeg_RNA_pol, 1 hit
PROSITEiView protein in PROSITE
PS50526, RDRP_SSRNA_NEG_NONSEG, 1 hit
PS51590, SAM_MT_MNV_L, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiL_HIRRV
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q77SJ8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: July 5, 2004
Last modified: October 7, 2020
This is version 78 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again