UniProtKB - Q77M43 (NCAP_MEASM)
Nucleoprotein
N
Functioni
Encapsidates the genome in a ratio of 1 N per 6 ribonucleotides, protecting it from nucleases. The nucleocapsid (NC) has a helical structure with either 12.35 or 11.64 N per turn, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest.
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
- structural molecule activity Source: InterPro
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding, Viral nucleoprotein |
Biological process | Host-virus interaction |
Names & Taxonomyi
Protein namesi | Recommended name: NucleoproteinAlternative name(s): Nucleocapsid protein Short name: NP Short name: Protein N |
Gene namesi | Name:N Synonyms:NP |
Organismi | Measles virus (strain Edmonston-Moraten vaccine) (MeV) (Subacute sclerose panencephalitis virus) |
Taxonomic identifieri | 132484 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Haploviricotina › Monjiviricetes › Mononegavirales › Paramyxoviridae › Orthoparamyxovirinae › Morbillivirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion Curated
- Host cytoplasm By similarity
Keywords - Cellular componenti
Capsid protein, Helical capsid protein, Host cytoplasm, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000142658 | 1 – 525 | NucleoproteinAdd BLAST | 525 |
Interactioni
Subunit structurei
Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. In nucleocapsid, binds the P protein and thereby positions the polymerase on the template.
Interacts with human FCGR2B protein (By similarity).
By similarityBinary interactionsi
Q77M43
With | #Exp. | IntAct |
---|---|---|
P [Q77M42] | 2 | EBI-21943629,EBI-21943616 |
Protein-protein interaction databases
IntActi | Q77M43, 1 interactor |
Structurei
Secondary structure
3D structure databases
BMRBi | Q77M43 |
SMRi | Q77M43 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q77M43 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 400 | NcoreBy similarityAdd BLAST | 400 | |
Regioni | 1 – 375 | HomomultimerizationAdd BLAST | 375 | |
Regioni | 401 – 525 | NtailBy similarityAdd BLAST | 125 | |
Regioni | 418 – 525 | DisorderedSequence analysisAdd BLAST | 108 | |
Regioni | 477 – 505 | P protein-bindingAdd BLAST | 29 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 428 – 456 | Basic and acidic residuesSequence analysisAdd BLAST | 29 |
Domaini
Sequence similaritiesi
Family and domain databases
InterProi | View protein in InterPro IPR002021, Paramyx_ncap |
Pfami | View protein in Pfam PF00973, Paramyxo_ncap, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MATLLRSLAL FKRNKDKPPI TSGSGGAIRG IKHIIIVPIP GDSSITTRSR
60 70 80 90 100
LLDRLVRLIG NPDVSGPKLT GALIGILSLF VESPGQLIQR ITDDPDVSIR
110 120 130 140 150
LLEVVQSDQS QSGLTFASRG TNMEDEADQY FSHDDPISSD QSRFGWFGNK
160 170 180 190 200
EISDIEVQDP EGFNMILGTI LAQIWVLLAK AVTAPDTAAD SELRRWIKYT
210 220 230 240 250
QQRRVVGEFR LERKWLDVVR NRIAEDLSLR RFMVALILDI KRTPGNKPRI
260 270 280 290 300
AEMICDIDTY IVEAGLASFI LTIKFGIETM YPALGLHEFA GELSTLESLM
310 320 330 340 350
NLYQQMGETA PYMVILENSI QNKFSAGSYP LLWSYAMGVG VELENSMGGL
360 370 380 390 400
NFGRSYFDPA YFRLGQEMVR RSAGKVSSTL ASELGITAED ARLVSEIAMH
410 420 430 440 450
TTEDKISRAV GPRQAQVSFL HGDQSENELP RLGGKEDRRV KQSRGEARES
460 470 480 490 500
YRETGPSRAS DARAAHLPTG TPLDIDTATE SSQDPQDSRR SADALLRLQA
510 520
MAGISEEQGS DTDTPIVYND RNLLD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U01999 mRNA Translation: AAA18998.1 AF266287 Genomic RNA Translation: AAF85667.1 |
PIRi | PQ0386 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U01999 mRNA Translation: AAA18998.1 AF266287 Genomic RNA Translation: AAF85667.1 |
PIRi | PQ0386 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1T6O | X-ray | 2.00 | B | 486-505 | [»] | |
6H5S | electron microscopy | 3.30 | C | 1-405 | [»] | |
BMRBi | Q77M43 | |||||
SMRi | Q77M43 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q77M43, 1 interactor |
Miscellaneous databases
EvolutionaryTracei | Q77M43 |
Family and domain databases
InterProi | View protein in InterPro IPR002021, Paramyx_ncap |
Pfami | View protein in Pfam PF00973, Paramyxo_ncap, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NCAP_MEASM | |
Accessioni | Q77M43Primary (citable) accession number: Q77M43 Secondary accession number(s): Q89794 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | September 29, 2021 | |
This is version 71 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families