UniProtKB - Q76LT8 (UBP48_RAT)
Protein
Ubiquitin carboxyl-terminal hydrolase 48
Gene
Usp48
Organism
Rattus norvegicus (Rat)
Status
Functioni
Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites.1 Publication
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 98 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 353 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: GO_Central
- thiol-dependent ubiquitin-specific protease activity Source: GO_Central
GO - Biological processi
- protein deubiquitination Source: GO_Central
- ubiquitin-dependent protein catabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-RNO-5689880, Ub-specific processing proteases |
Protein family/group databases
MEROPSi | C19.068 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin carboxyl-terminal hydrolase 48 (EC:3.4.19.12)Alternative name(s): Deubiquitinating enzyme 48 Synaptic ubiquitin-specific protease Short name: synUSP Ubiquitin thioesterase 48 Ubiquitin-specific-processing protease 48 |
Gene namesi | Name:Usp48 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 735213, Usp48 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- postsynaptic membrane Source: RGD
Other locations
- membrane raft Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249525 | 1 – 1036 | Ubiquitin carboxyl-terminal hydrolase 48Add BLAST | 1036 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 887 | PhosphoserineCombined sources | 1 | |
Modified residuei | 888 | PhosphoserineCombined sources | 1 | |
Modified residuei | 889 | PhosphoserineCombined sources | 1 | |
Modified residuei | 957 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q76LT8 |
PTM databases
iPTMneti | Q76LT8 |
PhosphoSitePlusi | Q76LT8 |
Expressioni
Tissue specificityi
Present in the brain, in particular in the postsynaptic density and the dendritic lipid raft fractions (at protein level).1 Publication
Interactioni
Subunit structurei
Interacts with TRAF2 and RELA.
By similarityProtein-protein interaction databases
BioGRIDi | 263601, 1 interactor |
STRINGi | 10116.ENSRNOP00000030008 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 89 – 421 | USPAdd BLAST | 333 | |
Domaini | 460 – 554 | DUSP 1PROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 569 – 692 | DUSP 2PROSITE-ProRule annotationAdd BLAST | 124 | |
Domaini | 712 – 825 | DUSP 3PROSITE-ProRule annotationAdd BLAST | 114 | |
Domaini | 930 – 1010 | Ubiquitin-likePROSITE-ProRule annotationAdd BLAST | 81 |
Sequence similaritiesi
Belongs to the peptidase C19 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1863, Eukaryota |
InParanoidi | Q76LT8 |
OrthoDBi | 113272at2759 |
PhylomeDBi | Q76LT8 |
Family and domain databases
CDDi | cd02668, Peptidase_C19L, 1 hit |
InterProi | View protein in InterPro IPR035927, DUSP-like_sf IPR038765, Papain-like_cys_pep_sf IPR006615, Pept_C19_DUSP IPR001394, Peptidase_C19_UCH IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR033841, USP48 IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF00443, UCH, 1 hit |
SUPFAMi | SSF143791, SSF143791, 1 hit SSF54001, SSF54001, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS51283, DUSP, 3 hits PS50053, UBIQUITIN_2, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q76LT8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPRLQLEKA AWRWAETVRP EEVSQEHIET AYRIWLEPCI RGVCRRNCRG
60 70 80 90 100
NPNCLVGIGE HIWLGEIDEN SFHNIDDPNC ERRKKNSFVG LTNLGASCYV
110 120 130 140 150
NTFLQVWFLN LELRQALYLC PSTCSDYTKG DGIRGGKDYE PQTICEHLQY
160 170 180 190 200
LFALLQNSNR RYIDPSGFVK ALGLDTGQQQ DAQESSKLFM SLLEDTLSKQ
210 220 230 240 250
KNPDVRNVVQ QQFCGEYAYV TVCSQCGRES KLVSKFYELE LNIQGHKQLT
260 270 280 290 300
DCISEFLKEE RLEGDNRYFC ENCQSKQNAT RKIRLLSLPC TLNLQLMRFV
310 320 330 340 350
FDRQTGHKKK LNAYIGFSES LDMEPYVEHK GGSFVYELSA VLIHRGVSAY
360 370 380 390 400
SGHYIAHVKD PQSGDWYKFN DEDIEKMEGK KLQLGIEEDL TEPSKSQTRK
410 420 430 440 450
PKCGKGTHCS RNAYMLVYRL QTQEKNHTMV QVPAFLQELV DRDNSKFEEW
460 470 480 490 500
CVEMAEMRRQ SVDKGRAKHE EVKELYQRLP AGAEPYEFVS LEWLQKWLDE
510 520 530 540 550
STPTKPIDNN ACLCSHDKLH PDKISIMKRI SEYAADIFYS RYGGGPRLTV
560 570 580 590 600
KALCKDCVVE RCRILRLKNQ LNEDYKTVNN LLKATMKGSD GFWVGKSSLR
610 620 630 640 650
SWRQLALEQL DEQDGEAEQS NGKINGSPFS KDESKEEKKE EEEELNFNED
660 670 680 690 700
ILCPHGELSI SENERRLVSQ EAWSKLQQYF PKAPEFPSYK ECCSQCKILE
710 720 730 740 750
REGEENEALH KMIAKEQKTS LPNLFQDKNR PCLSNWPEDT DALYIVSHFF
760 770 780 790 800
LDEWRKFVRK PARSTPVSSV GNAALLCPHG GLMFTFPSLT KEDSKLIALI
810 820 830 840 850
WPSEWQMIQK LFVVDKVIKI TRIEVGDVNP SQTQYISEPN LCPDCREGLL
860 870 880 890 900
CQQQKDLREY TQATIYVHKV VDNKKVMKDS APELNVSSSE TEEDKEEAKP
910 920 930 940 950
DGEKDPDFNQ SNGGTKRQKT SQQGYVAYQK QVIRRSTRHR KVRGEKALLV
960 970 980 990 1000
SANQTLKELK IQIMHAFSVA PFDQNLSIDG KILNDDCATL GTLGVIPESV
1010 1020 1030
ILLKADEPIA DYAAMDDVMQ VCMPEEGFKG TGLLGH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JUQ8 | A0A0G2JUQ8_RAT | Ubiquitinyl hydrolase 1 | Usp48 | 1,063 | Annotation score: | ||
F1M9B3 | F1M9B3_RAT | Ubiquitinyl hydrolase 1 | Usp48 | 1,036 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB073880 mRNA Translation: BAD00009.1 |
RefSeqi | NP_942080.1, NM_198785.1 |
Genome annotation databases
GeneIDi | 362636 |
KEGGi | rno:362636 |
UCSCi | RGD:735213, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB073880 mRNA Translation: BAD00009.1 |
RefSeqi | NP_942080.1, NM_198785.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 263601, 1 interactor |
STRINGi | 10116.ENSRNOP00000030008 |
Protein family/group databases
MEROPSi | C19.068 |
PTM databases
iPTMneti | Q76LT8 |
PhosphoSitePlusi | Q76LT8 |
Proteomic databases
PaxDbi | Q76LT8 |
Genome annotation databases
GeneIDi | 362636 |
KEGGi | rno:362636 |
UCSCi | RGD:735213, rat |
Organism-specific databases
CTDi | 84196 |
RGDi | 735213, Usp48 |
Phylogenomic databases
eggNOGi | KOG1863, Eukaryota |
InParanoidi | Q76LT8 |
OrthoDBi | 113272at2759 |
PhylomeDBi | Q76LT8 |
Enzyme and pathway databases
Reactomei | R-RNO-5689880, Ub-specific processing proteases |
Miscellaneous databases
PROi | PR:Q76LT8 |
Family and domain databases
CDDi | cd02668, Peptidase_C19L, 1 hit |
InterProi | View protein in InterPro IPR035927, DUSP-like_sf IPR038765, Papain-like_cys_pep_sf IPR006615, Pept_C19_DUSP IPR001394, Peptidase_C19_UCH IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR033841, USP48 IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF00443, UCH, 1 hit |
SUPFAMi | SSF143791, SSF143791, 1 hit SSF54001, SSF54001, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS51283, DUSP, 3 hits PS50053, UBIQUITIN_2, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBP48_RAT | |
Accessioni | Q76LT8Primary (citable) accession number: Q76LT8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2006 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries