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Protein

RNA1 polyprotein

Gene

RNA1

Organism
Broad bean wilt virus 1 (strain Spinach/United States/ATCC PV-132/1963) (BBWV-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Picornain 3C-like protease is a thiol protease that probably cleaves the B and M polyproteins.By similarity
VPg plays a role in RNA replication.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei975For picornain 3C-like protease activitySequence analysis1
Active sitei1011For picornain 3C-like protease activitySequence analysis1
Active sitei1100For picornain 3C-like protease activitySequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi487 – 494ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA1 polyprotein
Alternative name(s):
Genome polyprotein B
Cleaved into the following 5 chains:
Alternative name(s):
Membrane-binding protein
NTP-binding protein
Short name:
NTB
Alternative name(s):
VPg
Picornain 3C-like protease (EC:3.4.22.-)
Short name:
3C-like protease
Gene namesi
Name:RNA1
OrganismiBroad bean wilt virus 1 (strain Spinach/United States/ATCC PV-132/1963) (BBWV-1)
Taxonomic identifieri649895 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stagePicornaviralesSecoviridaeComovirinaeFabavirus
Virus hostiCapsicum annuum (Bell pepper) [TaxID: 4072]
Myzus [TaxID: 13163]
Petunia [TaxID: 4101]
Spinacia oleracea (Spinach) [TaxID: 3562]
Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906]
Proteomesi
  • UP000000409 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004027811 – 1842RNA1 polyproteinAdd BLAST1842
ChainiPRO_00004027821 – 316Protease cofactorSequence analysisAdd BLAST316
ChainiPRO_0000402783317 – 908Putative helicaseSequence analysisAdd BLAST592
ChainiPRO_0000402784909 – 934Viral genome-linked proteinSequence analysisAdd BLAST26
ChainiPRO_0000402785935 – 1141Picornain 3C-like proteaseSequence analysisAdd BLAST207
ChainiPRO_00004027861142 – 1842RNA-directed RNA polymeraseSequence analysisAdd BLAST701

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei909O-(5'-phospho-RNA)-serineBy similarity1

Post-translational modificationi

Specific enzymatic cleavages by picornain 3C-like protease in vivo yield mature proteins. Picornain 3C-like protease is autocatalytically processed (By similarity).By similarity
VPg is uridylylated by the polymerase and is covalently linked to the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei316 – 317CleavageSequence analysis2
Sitei909 – 910CleavageSequence analysis2
Sitei934 – 935CleavageSequence analysis2
Sitei1141 – 1142CleavageSequence analysis2

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Proteomic databases

PRIDEiQ76L40

Structurei

3D structure databases

ProteinModelPortaliQ76L40
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini458 – 626SF3 helicasePROSITE-ProRule annotationAdd BLAST169
Domaini967 – 1123Peptidase C3Add BLAST157
Domaini1417 – 1544RdRp catalyticPROSITE-ProRule annotationAdd BLAST128

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi333 – 339Poly-Ser7

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG0900006M

Family and domain databases

InterProiView protein in InterPro
IPR004004 Helic/Pol/Pept_Calicivir-typ
IPR000605 Helicase_SF3_ssDNA/RNA_vir
IPR014759 Helicase_SF3_ssRNA_vir
IPR000199 Peptidase_C3A/C3B_picornavir
IPR009003 Peptidase_S1_PA
IPR001205 RNA-dir_pol_C
IPR007094 RNA-dir_pol_PSvirus
PfamiView protein in Pfam
PF00548 Peptidase_C3, 1 hit
PF00680 RdRP_1, 1 hit
PF00910 RNA_helicase, 1 hit
PRINTSiPR00918 CALICVIRUSNS
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50507 RDRP_SSRNA_POS, 1 hit
PS51218 SF3_HELICASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q76L40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSETIDMCV KFLKISFGLQ SLKNLVKELF GGSELEKLAY VHAAFFHANE
60 70 80 90 100
MAIHWNADLP WEEVMSSKRI KERFGYVKSH FLRNVVYNAD ASGQMIRYNT
110 120 130 140 150
TTCEQWFCCN FNLASYSASY NSLVPEEGGS MPINEEAEIK IGQSLLTCAQ
160 170 180 190 200
SVTKAIYAKL STLTTRSIQG FLECLRDAIC GAFSSWLPCI RGAFAWFGNI
210 220 230 240 250
IEVLKHWAGA AHEKLHNFLE GIEECLYMGL GLVASTCIVA LIEKFLVTMS
260 270 280 290 300
VISGPCGRPT LFLTSAMAII SSTYLLSKAV EKSSAFTMLL GFVTQSCQTV
310 320 330 340 350
LGSLFGKSAK GSEEAQGQFG PSAMLESLAT LVSSWSSSSV TEIGRTFGAI
360 370 380 390 400
SQIKNGIIAL KDMALFVFSK LCEMASKVLG FESQILADLS IILGENVADW
410 420 430 440 450
LDECDCMLAY LLEFNSNARD IFDRLSQLIE KGKAIRMGIL RTTHRGPSQV
460 470 480 490 500
LSLVTKALDK LTELHNSVIM SGANSTRKTP FMLFFTGKSG VGKTSVVQRM
510 520 530 540 550
AANWLQQEQL GSNEVYSRNG LDPFWSGYKR QAVVTYDDFG AVPGSVSNEA
560 570 580 590 600
EIINVVSSNP HSVMMADLKE KGMYFDSRLI IASSNFLAAN PESGVHDSEA
610 620 630 640 650
YERRRHVVVQ VSLKEDMAYD PGNPCANQRY TLLESKAPFA EKAVFESYEE
660 670 680 690 700
LWSHVYNAFK AHEEKEKLFL SSLPIPERSE KEALQALIGI CVMTTSYAPK
710 720 730 740 750
AVIQYGIDHL VGYHYLISSA EHVYFWHEKG EVEIVPMHLM KLDKMDKATM
760 770 780 790 800
ASTSLKSALM CQDMAKNFPT LNPLAVLYAK NIVIRGWVDA NLQASKKCED
810 820 830 840 850
SYMREQIESL PKWQRAYLHV LSGHIASNET RGWFLNCLEV TKSNSRSSYI
860 870 880 890 900
WEYKSWPMPL KLALGSFLAI LAGSAIFCSL QSLWSISGNA SFVAGAASIF
910 920 930 940 950
TIGSATAQSA PPNKDGSEYT YRNKKIKIRN WEGQGPCFGD SALWIAENCM
960 970 980 990 1000
ATLVVMKDRV QVCMAPGRSF LGVNHFLRMI PNGVMVKLET GMTETYFVWE
1010 1020 1030 1040 1050
KSKLKLFENS EIALYTSSNL PKAPDSLVDR FHFDLETLPK TFPAQFFTYK
1060 1070 1080 1090 1100
FDKDMQQYVP ELGELLCKKA ERALCVVSGE YRRVISHHLT YRNPTVAGDC
1110 1120 1130 1140 1150
GGLVLAIIEG KCKLVGLHVA SDGEEGAASP VPWDPDFKVA QGQSDFLLSY
1160 1170 1180 1190 1200
DEWAVPKVLG PGCKAVGIIS PEHTVGSGGK TSFLETPIEW QLNRPCGKIP
1210 1220 1230 1240 1250
SILVKGDVRL AGTENADYDP FAVGMTKYAK EAGPFEPNGL DRVCESIAET
1260 1270 1280 1290 1300
WHDASDGFEF GPVDLEAALN GIENMEYFDA LVLSTSEGYP YRLDRKPGEK
1310 1320 1330 1340 1350
GKARYVEGEP GNLEITDERI LADIHWFEEI SKTQVPDLYC IECVKDERLP
1360 1370 1380 1390 1400
VRKVIKEPKS RLFTVLPMSY NLAIRKKFLN FVRFIMKRRD VLPCQVGINP
1410 1420 1430 1440 1450
YSRQWGKVAD RLLEKGNSIL CCDYSRFDGF LPKCIMVKIA EMFSNIVGET
1460 1470 1480 1490 1500
GAEREQTKNL MLACCSRYAI CGRVLYRVEN GIPSGFPLTV IVNSILNEIL
1510 1520 1530 1540 1550
IKYAYWKCFE TESLIRDHFD TYVAMVVYGD DNLISVSEAI SSKFNGNFLV
1560 1570 1580 1590 1600
NFMCNLGIKV TDGVDKTKVG IEFRTIEDCD FLKRKFKENA DGTWSGVMAE
1610 1620 1630 1640 1650
EHLWPQLHFV KAKKVEMSEA YISNCNNILR ELWLGSPEKA AAFRREVISK
1660 1670 1680 1690 1700
LKWVEPQRLL TISQVALFHN EQMNGEHPFV EACHQLENLE LMAPLEPGML
1710 1720 1730 1740 1750
PIKTQEIMPG LFVASEKNFT GNFDDYFTIS ITTNRKFEDG KGFQIIFPYG
1760 1770 1780 1790 1800
AGRGGLPSKA FMEQNVIRKG CAIQKAFKQG LEKGNKMLFI SQSSVIPAYV
1810 1820 1830 1840
FAIMLYRSVD RLPRALSNKA LTSALGICKK LSYLPKDFPD LF
Length:1,842
Mass (Da):205,786
Last modified:July 5, 2004 - v1
Checksum:i1784AEFEAFA8DEB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB084450 Genomic RNA Translation: BAD00183.1
RefSeqiNP_945134.1, NC_005289.1

Genome annotation databases

GeneIDi2658942
KEGGivg:2658942

Similar proteinsi

Entry informationi

Entry nameiPOL1_BBWVS
AccessioniPrimary (citable) accession number: Q76L40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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