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UniProtKB - Q76L36 (CPRC2_CANPA)
Protein
NADPH-dependent conjugated polyketone reductase C2
Gene
cpr-c2
Organism
Candida parapsilosis (Yeast)
Status
Functioni
NADPH-dependent conjugated polyketone reductase with broad substrate specificity and strict stereospecificity. Reduces ketopantoyl lactone and isatin. Does not act on menadione, p-nitrobenzaldehyde and pyridine-3-aldehyde.
2 PublicationsCatalytic activityi
Activity regulationi
Inhibited by quercetin.1 Publication
Kineticsi
- KM=333 µM for ketopantoyl lactone2 Publications
- KM=14 µM for isatin2 Publications
- Vmax=481 µmol/min/mg enzyme with ketopantoyl lactone as substrate2 Publications
- Vmax=306 µmol/min/mg enzyme with isatin as substrate2 Publications
pH dependencei
Optimum pH is 7.2 Publications
Temperature dependencei
Optimum temperature is 40 degrees Celsius.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 63 | Proton donorBy similarity | 1 | |
Sitei | 88 | Lowers pKa of active site TyrBy similarity | 1 | |
Binding sitei | 125 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 215 – 271 | NADPBy similarityAdd BLAST | 57 |
GO - Molecular functioni
- 2-dehydropantolactone reductase (A-specific) activity Source: UniProtKB
- aldo-keto reductase (NADP) activity Source: CGD
- oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor Source: InterPro
GO - Biological processi
- cellular ketone metabolic process Source: CGD
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.214, 1133 1.1.1.358, 1133 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:cpr-c2 |
Organismi | Candida parapsilosis (Yeast) |
Taxonomic identifieri | 5480 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Candida/Lodderomyces clade › Candida |
Organism-specific databases
CGDi | CAL0000156263, CPR-C2 |
VEuPathDBi | FungiDB:CPAR2_703660 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418423 | 1 – 307 | NADPH-dependent conjugated polyketone reductase C2Add BLAST | 307 |
Post-translational modificationi
The N-terminus is blocked.
Interactioni
Subunit structurei
Monomer.
2 PublicationsStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q76L36 |
SMRi | Q76L36 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the aldo/keto reductase family.Curated
Family and domain databases
CDDi | cd19120, AKR_AKR3C2-3, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR020471, AKR IPR044494, AKR3C2/3 IPR018170, Aldo/ket_reductase_CS IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
Q76L36-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTQSNLLPKT FRTKSGKEIS IALGTGTKWK QAQTINDVST ELVDNILLGL
60 70 80 90 100
KLGFRHIDTA EAYNTQKEVG EALKRTDVPR EDIWVTTKYS PGWGSIKAYS
110 120 130 140 150
KSPSDSIDKA LAQLGVDYVD LFLIHSPFFT TEQTHGYTLE QAWEALVEAK
160 170 180 190 200
KAGKVREIGI SNAAIPHLEK LFAASPSPEY YPVVNQIEFH PFLQNQSKNI
210 220 230 240 250
VRFCQEHGIL VEAFSPLAPL ARVETNALAE TLKRLAEKYK KTEAQVLLRY
260 270 280 290 300
TLQRGILPVT TSSKESRLKE SLNLFDFELT DEEVNEINKI GDANPYRAFF
HEQFKDL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB084516 Genomic DNA Translation: BAD01653.1 JX512918 Genomic DNA Translation: AFQ61054.1 |
Genome annotation databases
KEGGi | ag:BAD01653 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB084516 Genomic DNA Translation: BAD01653.1 JX512918 Genomic DNA Translation: AFQ61054.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3VXG | X-ray | 1.70 | A | 1-307 | [»] | |
4H8N | X-ray | 1.80 | A/B | 1-307 | [»] | |
AlphaFoldDBi | Q76L36 | |||||
SMRi | Q76L36 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
KEGGi | ag:BAD01653 |
Organism-specific databases
CGDi | CAL0000156263, CPR-C2 |
VEuPathDBi | FungiDB:CPAR2_703660 |
Enzyme and pathway databases
BRENDAi | 1.1.1.214, 1133 1.1.1.358, 1133 |
Family and domain databases
CDDi | cd19120, AKR_AKR3C2-3, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR020471, AKR IPR044494, AKR3C2/3 IPR018170, Aldo/ket_reductase_CS IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CPRC2_CANPA | |
Accessioni | Q76L36Primary (citable) accession number: Q76L36 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2013 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families