UniProtKB - Q76I76 (SSH2_HUMAN)
Protein
Protein phosphatase Slingshot homolog 2
Gene
SSH2
Organism
Homo sapiens (Human)
Status
Functioni
Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.1 Publication
Miscellaneous
Tyrosine phosphatase activity has not been demonstrated for this protein to date.
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 392 | Phosphocysteine intermediatePROSITE-ProRule annotation1 Publication | 1 |
GO - Molecular functioni
- actin binding Source: UniProtKB
- phosphoprotein phosphatase activity Source: UniProtKB
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
- protein tyrosine/serine/threonine phosphatase activity Source: InterPro
- protein tyrosine phosphatase activity Source: UniProtKB-EC
GO - Biological processi
- actin cytoskeleton organization Source: UniProtKB
- negative regulation of actin filament polymerization Source: GO_Central
- protein dephosphorylation Source: UniProtKB
Keywordsi
Molecular function | Actin-binding, Hydrolase, Protein phosphatase |
Enzyme and pathway databases
PathwayCommonsi | Q76I76 |
SIGNORi | Q76I76 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:SSH2 Synonyms:KIAA1725, SSH2L |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30580, SSH2 |
MIMi | 606779, gene |
neXtProti | NX_Q76I76 |
VEuPathDBi | HostDB:ENSG00000141298.17 |
Subcellular locationi
Cytoskeleton
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular space Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 392 | C → S: Abrogates phosphatase activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 85464 |
OpenTargetsi | ENSG00000141298 |
PharmGKBi | PA134861867 |
Miscellaneous databases
Pharosi | Q76I76, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3351198 |
Genetic variation databases
BioMutai | SSH2 |
DMDMi | 74749833 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094843 | 1 – 1423 | Protein phosphatase Slingshot homolog 2Add BLAST | 1423 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 17 | PhosphoserineBy similarity | 1 | |
Modified residuei | 25 | PhosphoserineBy similarity | 1 | |
Modified residuei | 36 | PhosphoserineCombined sources | 1 | |
Modified residuei | 461 | PhosphoserineBy similarity | 1 | |
Modified residuei | 487 | PhosphoserineCombined sources | 1 | |
Modified residuei | 534 | PhosphoserineBy similarity | 1 | |
Modified residuei | 631 | PhosphoserineBy similarity | 1 | |
Modified residuei | 633 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1217 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1422 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q76I76 |
jPOSTi | Q76I76 |
MassIVEi | Q76I76 |
MaxQBi | Q76I76 |
PaxDbi | Q76I76 |
PeptideAtlasi | Q76I76 |
PRIDEi | Q76I76 |
ProteomicsDBi | 68671 [Q76I76-1] 68672 [Q76I76-2] 68673 [Q76I76-3] 68674 [Q76I76-4] |
PTM databases
DEPODi | SSH2 |
iPTMneti | Q76I76 |
PhosphoSitePlusi | Q76I76 |
Expressioni
Gene expression databases
Bgeei | ENSG00000141298, Expressed in quadriceps femoris and 210 other tissues |
ExpressionAtlasi | Q76I76, baseline and differential |
Genevisiblei | Q76I76, HS |
Organism-specific databases
HPAi | ENSG00000141298, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Interacts with filamentous actin.
1 PublicationGO - Molecular functioni
- actin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 124548, 35 interactors |
IntActi | Q76I76, 20 interactors |
STRINGi | 9606.ENSP00000444743 |
Chemistry databases
BindingDBi | Q76I76 |
Miscellaneous databases
RNActi | Q76I76, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q76I76 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q76I76 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 307 – 448 | Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST | 142 |
Sequence similaritiesi
Belongs to the protein-tyrosine phosphatase family.Curated
Phylogenomic databases
eggNOGi | KOG1716, Eukaryota |
GeneTreei | ENSGT00940000157430 |
InParanoidi | Q76I76 |
PhylomeDBi | Q76I76 |
TreeFami | TF319444 |
Family and domain databases
CDDi | cd11652, SSH-N, 1 hit |
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR014876, DEK_C IPR043587, Phosphatase_SSH-like IPR029021, Prot-tyrosine_phosphatase-like IPR043588, SSH-N IPR016130, Tyr_Pase_AS IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
PANTHERi | PTHR45864, PTHR45864, 1 hit |
Pfami | View protein in Pfam PF08766, DEK_C, 1 hit |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q76I76-1) [UniParc]FASTAAdd to basket
Also known as: L
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALVTVQRSP TPSTTSSPCA SEADSGEEEC RSQPRSISES FLTVKGAALF
60 70 80 90 100
LPRGNGSSTP RISHRRNKHA GDLQQHLQAM FILLRPEDNI RLAVRLESTY
110 120 130 140 150
QNRTRYMVVV STNGRQDTEE SIVLGMDFSS NDSSTCTMGL VLPLWSDTLI
160 170 180 190 200
HLDGDGGFSV STDNRVHIFK PVSVQAMWSA LQSLHKACEV ARAHNYYPGS
210 220 230 240 250
LFLTWVSYYE SHINSDQSSV NEWNAMQDVQ SHRPDSPALF TDIPTERERT
260 270 280 290 300
ERLIKTKLRE IMMQKDLENI TSKEIRTELE MQMVCNLREF KEFIDNEMIV
310 320 330 340 350
ILGQMDSPTQ IFEHVFLGSE WNASNLEDLQ NRGVRYILNV TREIDNFFPG
360 370 380 390 400
VFEYHNIRVY DEEATDLLAY WNDTYKFISK AKKHGSKCLV HCKMGVSRSA
410 420 430 440 450
STVIAYAMKE YGWNLDRAYD YVKERRTVTK PNPSFMRQLE EYQGILLASK
460 470 480 490 500
QRHNKLWRSH SDSDLSDHHE PICKPGLELN KKDITTSADQ IAEVKTMESH
510 520 530 540 550
PPIPPVFVEH MVPQDANQKG LCTKERMICL EFTSREFHAG QIEDELNLND
560 570 580 590 600
INGCSSGCCL NESKFPLDNC HASKALIQPG HVPEMANKFP DLTVEDLETD
610 620 630 640 650
ALKADMNVHL LPMEELTSPL KDPPMSPDPE SPSPQPSCQT EISDFSTDRI
660 670 680 690 700
DFFSALEKFV ELSQETRSRS FSHSRMEELG GGRNESCRLS VVEVAPSKVT
710 720 730 740 750
ADDQRSSSLS NTPHASEESS MDEEQSKAIS ELVSPDIFMQ SHSENAISVK
760 770 780 790 800
EIVTEIESIS QGVGQIQLKG DILPNPCHTP KKNSIHELLL ERAQTPENKP
810 820 830 840 850
GHMEQDEDSC TAQPELAKDS GMCNPEGCLT THSSIADLEE GEPAEGEQEL
860 870 880 890 900
QGSGMHPGAK WYPGSVRRAT LEFEERLRQE QEHHGAAPTC TSLSTRKNSK
910 920 930 940 950
NDSSVADLAP KGKSDEAPPE HSFVLKEPEM SKGKGKYSGS EAGSLSHSEQ
960 970 980 990 1000
NATVPAPRVL EFDHLPDPQE GPGSDTGTQQ EGVLKDLRTV IPYQESETQA
1010 1020 1030 1040 1050
VPLPLPKRVE IIEYTHIVTS PNHTGPGSEI ATSEKSGEQG LRKVNMEKSV
1060 1070 1080 1090 1100
TVLCTLDENL NRTLDPNQVS LHPQVLPLPH SSSPEHNRPT DHPTSILSSP
1110 1120 1130 1140 1150
EDRGSSLSTA LETAAPFVSH TTHLLSASLD YLHPQTMVHL EGFTEQSSTT
1160 1170 1180 1190 1200
DEPSAEQVSW EESQESPLSS GSEVPYKDSQ LSSADLSLIS KLGDNTGELQ
1210 1220 1230 1240 1250
EKMDPLPVAC RLPHSSSSEN IKSLSHSPGV VKERAKEIES RVVFQAGLTK
1260 1270 1280 1290 1300
PSQMRRSASL AKLGYLDLCK DCLPEREPAS CESPHLKLLQ PFLRTDSGMH
1310 1320 1330 1340 1350
AMEDQESLEN PGAPHNPEPT KSFVEQLTTT ECIVQSKPVE RPLVQYAKEF
1360 1370 1380 1390 1400
GSSQQYLLPR AGLELTSSEG GLPVLQTQGL QCACPAPGLA VAPRQQHGRT
1410 1420
HPLRRLKKAN DKKRTTNPFY NTM
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5H527 | F5H527_HUMAN | Protein phosphatase Slingshot homol... | SSH2 | 1,450 | Annotation score: | ||
A0A3B3IS79 | A0A3B3IS79_HUMAN | Protein phosphatase Slingshot homol... | SSH2 | 1,429 | Annotation score: | ||
J3KSQ9 | J3KSQ9_HUMAN | Protein phosphatase Slingshot homol... | SSH2 | 112 | Annotation score: | ||
K7EKN7 | K7EKN7_HUMAN | Protein phosphatase Slingshot homol... | SSH2 | 116 | Annotation score: | ||
K7EP00 | K7EP00_HUMAN | Protein phosphatase Slingshot homol... | SSH2 | 120 | Annotation score: | ||
A0A3B3ITS1 | A0A3B3ITS1_HUMAN | Protein phosphatase Slingshot homol... | SSH2 hCG_2003225 | 202 | Annotation score: | ||
J3KSP5 | J3KSP5_HUMAN | Protein phosphatase Slingshot homol... | SSH2 | 48 | Annotation score: | ||
K7EQZ0 | K7EQZ0_HUMAN | Protein phosphatase Slingshot homol... | SSH2 | 42 | Annotation score: |
Sequence cautioni
The sequence AAH11636 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAL92027 differs from that shown. Reason: Erroneous initiation.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051758 | 743 | S → L. Corresponds to variant dbSNP:rs2289629Ensembl. | 1 | |
Natural variantiVAR_051759 | 763 | V → A. Corresponds to variant dbSNP:rs6505140Ensembl. | 1 | |
Natural variantiVAR_051760 | 1300 | H → Q. Corresponds to variant dbSNP:rs8080046Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016321 | 1 – 36 | MALVT…SQPRS → MTLSTLARKRKAPLACTCSL GGPDMIPYFSANAVISQNAI NQL in isoform 2. 1 PublicationAdd BLAST | 36 | |
Alternative sequenceiVSP_016322 | 179 – 195 | SALQS…ARAHN → VDRDSRNKHCYVLLVEE in isoform 2 and isoform 3. 2 PublicationsAdd BLAST | 17 | |
Alternative sequenceiVSP_016323 | 196 – 1423 | Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST | 1228 | |
Alternative sequenceiVSP_016324 | 449 | S → R in isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_016325 | 450 – 1423 | Missing in isoform 4. 1 PublicationAdd BLAST | 974 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB072358 mRNA Translation: BAB84117.1 AB072359 mRNA Translation: BAB84118.1 AB099290 mRNA Translation: BAC97813.1 BC008941 mRNA Translation: AAH08941.1 BC011636 mRNA Translation: AAH11636.2 Different initiation. BC068223 mRNA Translation: AAH68223.1 AF484838 mRNA Translation: AAL92027.1 Different initiation. AB051512 mRNA Translation: BAB21816.1 |
CCDSi | CCDS11253.1 [Q76I76-1] |
RefSeqi | NP_001269058.1, NM_001282129.1 NP_001269059.1, NM_001282130.1 NP_001269060.1, NM_001282131.1 NP_203747.2, NM_033389.3 [Q76I76-1] |
Genome annotation databases
Ensembli | ENST00000269033; ENSP00000269033; ENSG00000141298 [Q76I76-1] |
GeneIDi | 85464 |
KEGGi | hsa:85464 |
UCSCi | uc002heo.3, human [Q76I76-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB072358 mRNA Translation: BAB84117.1 AB072359 mRNA Translation: BAB84118.1 AB099290 mRNA Translation: BAC97813.1 BC008941 mRNA Translation: AAH08941.1 BC011636 mRNA Translation: AAH11636.2 Different initiation. BC068223 mRNA Translation: AAH68223.1 AF484838 mRNA Translation: AAL92027.1 Different initiation. AB051512 mRNA Translation: BAB21816.1 |
CCDSi | CCDS11253.1 [Q76I76-1] |
RefSeqi | NP_001269058.1, NM_001282129.1 NP_001269059.1, NM_001282130.1 NP_001269060.1, NM_001282131.1 NP_203747.2, NM_033389.3 [Q76I76-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2NT2 | X-ray | 2.10 | A/B/C | 305-449 | [»] | |
SMRi | Q76I76 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124548, 35 interactors |
IntActi | Q76I76, 20 interactors |
STRINGi | 9606.ENSP00000444743 |
Chemistry databases
BindingDBi | Q76I76 |
ChEMBLi | CHEMBL3351198 |
PTM databases
DEPODi | SSH2 |
iPTMneti | Q76I76 |
PhosphoSitePlusi | Q76I76 |
Genetic variation databases
BioMutai | SSH2 |
DMDMi | 74749833 |
Proteomic databases
EPDi | Q76I76 |
jPOSTi | Q76I76 |
MassIVEi | Q76I76 |
MaxQBi | Q76I76 |
PaxDbi | Q76I76 |
PeptideAtlasi | Q76I76 |
PRIDEi | Q76I76 |
ProteomicsDBi | 68671 [Q76I76-1] 68672 [Q76I76-2] 68673 [Q76I76-3] 68674 [Q76I76-4] |
Protocols and materials databases
Antibodypediai | 26804, 52 antibodies |
DNASUi | 85464 |
Genome annotation databases
Ensembli | ENST00000269033; ENSP00000269033; ENSG00000141298 [Q76I76-1] |
GeneIDi | 85464 |
KEGGi | hsa:85464 |
UCSCi | uc002heo.3, human [Q76I76-1] |
Organism-specific databases
CTDi | 85464 |
DisGeNETi | 85464 |
GeneCardsi | SSH2 |
HGNCi | HGNC:30580, SSH2 |
HPAi | ENSG00000141298, Tissue enhanced (skeletal) |
MIMi | 606779, gene |
neXtProti | NX_Q76I76 |
OpenTargetsi | ENSG00000141298 |
PharmGKBi | PA134861867 |
VEuPathDBi | HostDB:ENSG00000141298.17 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1716, Eukaryota |
GeneTreei | ENSGT00940000157430 |
InParanoidi | Q76I76 |
PhylomeDBi | Q76I76 |
TreeFami | TF319444 |
Enzyme and pathway databases
PathwayCommonsi | Q76I76 |
SIGNORi | Q76I76 |
Miscellaneous databases
BioGRID-ORCSi | 85464, 17 hits in 991 CRISPR screens |
ChiTaRSi | SSH2, human |
EvolutionaryTracei | Q76I76 |
GeneWikii | SSH2 |
GenomeRNAii | 85464 |
Pharosi | Q76I76, Tbio |
PROi | PR:Q76I76 |
RNActi | Q76I76, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000141298, Expressed in quadriceps femoris and 210 other tissues |
ExpressionAtlasi | Q76I76, baseline and differential |
Genevisiblei | Q76I76, HS |
Family and domain databases
CDDi | cd11652, SSH-N, 1 hit |
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR014876, DEK_C IPR043587, Phosphatase_SSH-like IPR029021, Prot-tyrosine_phosphatase-like IPR043588, SSH-N IPR016130, Tyr_Pase_AS IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
PANTHERi | PTHR45864, PTHR45864, 1 hit |
Pfami | View protein in Pfam PF08766, DEK_C, 1 hit |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SSH2_HUMAN | |
Accessioni | Q76I76Primary (citable) accession number: Q76I76 Secondary accession number(s): Q8TDB5 Q9C0D8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | April 7, 2021 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families