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UniProtKB - Q76EQ0 (SRR_RAT)
Protein
Serine racemase
Gene
Srr
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
2 PublicationsCatalytic activityi
Cofactori
pyridoxal 5'-phosphate1 Publication
Activity regulationi
Allosterically activated by magnesium, and possibly also other divalent metal cations. Allosterically activated by ATP, ADP or GTP (By similarity). Competitively inhibited by malonate.By similarity1 Publication
Kineticsi
- KM=3.7 mM for L-serine1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 51 | ATPBy similarity | 1 | |
Active sitei | 56 | Proton acceptorBy similarity | 1 | |
Active sitei | 84 | Proton acceptorBy similarity | 1 | |
Binding sitei | 121 | ATPBy similarity | 1 | |
Binding sitei | 135 | SubstrateCurated | 1 | |
Metal bindingi | 210 | MagnesiumBy similarity | 1 | |
Metal bindingi | 214 | Magnesium; via carbonyl oxygen | 1 | |
Metal bindingi | 216 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- calcium ion binding Source: RGD
- D-serine ammonia-lyase activity Source: UniProtKB-EC
- glycine binding Source: RGD
- identical protein binding Source: RGD
- L-serine ammonia-lyase activity Source: RGD
- magnesium ion binding Source: UniProtKB
- PDZ domain binding Source: RGD
- protein homodimerization activity Source: UniProtKB
- pyridoxal phosphate binding Source: UniProtKB
- serine racemase activity Source: UniProtKB
- threonine racemase activity Source: RGD
GO - Biological processi
- aging Source: RGD
- brain development Source: RGD
- D-serine biosynthetic process Source: RGD
- D-serine metabolic process Source: UniProtKB
- L-serine metabolic process Source: UniProtKB
- pyruvate biosynthetic process Source: RGD
- response to lipopolysaccharide Source: RGD
- response to morphine Source: RGD
- response to organic cyclic compound Source: RGD
- response to xenobiotic stimulus Source: RGD
- serine family amino acid metabolic process Source: RGD
Keywordsi
Molecular function | Allosteric enzyme, Isomerase, Lyase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Pyridoxal phosphate |
Enzyme and pathway databases
BRENDAi | 5.1.1.18, 5301 |
Reactomei | R-RNO-977347, Serine biosynthesis |
SABIO-RKi | Q76EQ0 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Srr |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 735094, Srr |
Subcellular locationi
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- apical part of cell Source: RGD
- cytoplasm Source: RGD
- neuronal cell body Source: RGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000286173 | 1 – 333 | Serine racemaseAdd BLAST | 333 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 56 | N6-(pyridoxal phosphate)lysine | 1 | |
Modified residuei | 71 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 113 | S-nitrosocysteineBy similarity | 1 |
Post-translational modificationi
S-nitrosylated, leading to decrease the enzyme activity.By similarity
Keywords - PTMi
Phosphoprotein, S-nitrosylationProteomic databases
PaxDbi | Q76EQ0 |
PTM databases
iPTMneti | Q76EQ0 |
PhosphoSitePlusi | Q76EQ0 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000002991, Expressed in liver and 21 other tissues |
ExpressionAtlasi | Q76EQ0, baseline and differential |
Genevisiblei | Q76EQ0, RN |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: RGD
- PDZ domain binding Source: RGD
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 257487, 2 interactors |
STRINGi | 10116.ENSRNOP00000050774 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q76EQ0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q76EQ0 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 238 – 239 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the serine/threonine dehydratase family.Curated
Phylogenomic databases
eggNOGi | KOG1251, Eukaryota |
GeneTreei | ENSGT00550000075026 |
HOGENOMi | CLU_021152_4_2_1 |
InParanoidi | Q76EQ0 |
OMAi | LIHPFDH |
OrthoDBi | 943371at2759 |
PhylomeDBi | Q76EQ0 |
TreeFami | TF313346 |
Family and domain databases
Gene3Di | 3.40.50.1100, 2 hits |
InterProi | View protein in InterPro IPR001926, PLP-dep IPR000634, Ser/Thr_deHydtase_PyrdxlP-BS IPR036052, Trypto_synt_PLP_dependent |
Pfami | View protein in Pfam PF00291, PALP, 1 hit |
SUPFAMi | SSF53686, SSF53686, 1 hit |
PROSITEi | View protein in PROSITE PS00165, DEHYDRATASE_SER_THR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q76EQ0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MCAQYCISFA DVEKAHLNIQ DSVHLTPVLT SSILNQIAGR NLFFKCELFQ
60 70 80 90 100
KTGSFKIRGA LNAIRGLIPD TLEGKPKAVV THSSGNHGQA LTYAAKLEGI
110 120 130 140 150
PAYIVVPQTA PNCKKLAIQA YGASIVYSEP SDESRENVAQ RIIQETEGIL
160 170 180 190 200
VHPNQEPAVI AGQGTIALEV LNQVPLVDAL VVPVGGGGMV AGIAITIKTL
210 220 230 240 250
KPSVKVYAAE PSNADDCYQS KLKGELTPNL HPPETIADGV KSSIGLNTWP
260 270 280 290 300
IIRDLVDDVF TVTEDEIKYA TQLVWERMKL LIEPTAGVGL AAVLSQHFQT
310 320 330
VSPEVKNICI VLSGGNVDLT SLSWVKQAER PAP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0U1RRW0 | A0A0U1RRW0_RAT | Serine racemase | Srr | 168 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB106282 mRNA Translation: BAC84968.1 BC082014 mRNA Translation: AAH82014.1 |
RefSeqi | NP_942052.1, NM_198757.2 XP_008766237.1, XM_008768015.2 |
Genome annotation databases
Ensembli | ENSRNOT00000046110; ENSRNOP00000050774; ENSRNOG00000002991 ENSRNOT00000085394; ENSRNOP00000070927; ENSRNOG00000002991 |
GeneIDi | 303306 |
KEGGi | rno:303306 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB106282 mRNA Translation: BAC84968.1 BC082014 mRNA Translation: AAH82014.1 |
RefSeqi | NP_942052.1, NM_198757.2 XP_008766237.1, XM_008768015.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3HMK | X-ray | 2.10 | A/B | 1-333 | [»] | |
3L6C | X-ray | 2.20 | A/B | 1-333 | [»] | |
SMRi | Q76EQ0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 257487, 2 interactors |
STRINGi | 10116.ENSRNOP00000050774 |
PTM databases
iPTMneti | Q76EQ0 |
PhosphoSitePlusi | Q76EQ0 |
Proteomic databases
PaxDbi | Q76EQ0 |
Genome annotation databases
Ensembli | ENSRNOT00000046110; ENSRNOP00000050774; ENSRNOG00000002991 ENSRNOT00000085394; ENSRNOP00000070927; ENSRNOG00000002991 |
GeneIDi | 303306 |
KEGGi | rno:303306 |
Organism-specific databases
CTDi | 63826 |
RGDi | 735094, Srr |
Phylogenomic databases
eggNOGi | KOG1251, Eukaryota |
GeneTreei | ENSGT00550000075026 |
HOGENOMi | CLU_021152_4_2_1 |
InParanoidi | Q76EQ0 |
OMAi | LIHPFDH |
OrthoDBi | 943371at2759 |
PhylomeDBi | Q76EQ0 |
TreeFami | TF313346 |
Enzyme and pathway databases
BRENDAi | 5.1.1.18, 5301 |
Reactomei | R-RNO-977347, Serine biosynthesis |
SABIO-RKi | Q76EQ0 |
Miscellaneous databases
EvolutionaryTracei | Q76EQ0 |
PROi | PR:Q76EQ0 |
Gene expression databases
Bgeei | ENSRNOG00000002991, Expressed in liver and 21 other tissues |
ExpressionAtlasi | Q76EQ0, baseline and differential |
Genevisiblei | Q76EQ0, RN |
Family and domain databases
Gene3Di | 3.40.50.1100, 2 hits |
InterProi | View protein in InterPro IPR001926, PLP-dep IPR000634, Ser/Thr_deHydtase_PyrdxlP-BS IPR036052, Trypto_synt_PLP_dependent |
Pfami | View protein in Pfam PF00291, PALP, 1 hit |
SUPFAMi | SSF53686, SSF53686, 1 hit |
PROSITEi | View protein in PROSITE PS00165, DEHYDRATASE_SER_THR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SRR_RAT | |
Accessioni | Q76EQ0Primary (citable) accession number: Q76EQ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 23, 2022 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families