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Protein

Ice-binding protein K3-B1

Gene

K3-B1

Organism
Typhula ishikariensis (Gray snow mold fungus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the surface of ice crystals. Has low thermal hysteresis (TH) activity, which is the ability to lower the freezing point of an aqueous solution below its melting point (PubMed:22645341). The TH activity of this protein is approximately 0.3 degrees Celsius at 11 mM (PubMed:27613857).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei198Ice-binding1 Publication1

Names & Taxonomyi

Protein namesi
Recommended name:
Ice-binding protein K3-B1Curated
Alternative name(s):
Antifreeze protein 61 Publication
Short name:
AFP6Curated
TAFP-21 Publication
TisAFP61 Publication
Gene namesi
Name:K3-B1Imported
OrganismiTyphula ishikariensis (Gray snow mold fungus)
Taxonomic identifieri69361 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesThelephoralesTyphulaceaeTyphula

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi102T → Y: Has 85% thermal hysteresis (TH) activity of that of the wild-type. 1 Publication1
Mutagenesisi198N → Y: Has 40% thermal hysteresis (TH) activity of that of the wild-type. 1 Publication1
Mutagenesisi232S → A: Slight increase in thermal hysteresis (TH) activity (0.48 degrees Celsius) compared with that of the wild-type (0.32 degrees Celsius) at 0.14 mM. 1 Publication1
Mutagenesisi241V → Y: Has 85% thermal hysteresis (TH) activity of that of the wild-type. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 201 PublicationAdd BLAST20
ChainiPRO_500428714621 – 243Ice-binding protein K3-B1Sequence analysisAdd BLAST223

Expressioni

Inductioni

By low temperatures, 0 degree Celsius.1 Publication

Structurei

Secondary structure

1243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiQ76CE6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ice-binding protein family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR021884 Ice-bd_prot-like
PfamiView protein in Pfam
PF11999 DUF3494, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q76CE6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFSASSLLAV IALAISSVSA AGPSAVPLGT AGNYVILAST GVSTVPQSVI
60 70 80 90 100
TGAVGVSPGT AASLTGFSLI LSGTGTFSTS SQVTGQLTGA DYGTPTPSIL
110 120 130 140 150
TTAIGDMGTA YINAATRSGP DFLEIYTGAL GGTTLLPGLY KWTSSVGASA
160 170 180 190 200
DFTISGTSTD TWIFQIDGTL DVATGKQITL VGGAQAKNII WVVAGAVNIE
210 220 230 240
VGAKFEGTIL AKTAVTFKTG SSLNGRILAQ TSVALQSATI VEK
Length:243
Mass (Da):24,093
Last modified:July 5, 2004 - v1
Checksum:i063D02E6B34AADDD
GO

Mass spectrometryi

Molecular mass is 21978 Da from positions 21 - 243. Determined by MALDI. The measured mass is that of the mature protein.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB109744 mRNA Translation: BAD02893.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB109744 mRNA Translation: BAD02893.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VN3X-ray0.95A/B21-243[»]
SMRiQ76CE6
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiView protein in InterPro
IPR021884 Ice-bd_prot-like
PfamiView protein in Pfam
PF11999 DUF3494, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIBPKB_TYPIS
AccessioniPrimary (citable) accession number: Q76CE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 18, 2018
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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