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UniProtKB - Q75K78 (CKX9_ORYSJ)
Protein
Cytokinin dehydrogenase 9
Gene
CKX9
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Catalyzes the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group.
By similarityCatalytic activityi
- EC:1.5.99.12
Cofactori
FADBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 105 | FAD; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 161 | FAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 166 | FADBy similarity | 1 | |
Binding sitei | 227 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 469 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 95 – 99 | FADBy similarity | 5 | |
Nucleotide bindingi | 100 – 101 | FADBy similarity | 2 | |
Nucleotide bindingi | 172 – 176 | FADBy similarity | 5 | |
Nucleotide bindingi | 504 – 507 | FADBy similarity | 4 |
GO - Molecular functioni
- cytokinin dehydrogenase activity Source: UniProtKB-EC
- FAD binding Source: InterPro
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- cytokinin metabolic process Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Names & Taxonomyi
Protein namesi | Recommended name: Cytokinin dehydrogenase 9 (EC:1.5.99.12)Alternative name(s): Cytokinin oxidase 9 Short name: OsCKX9 |
Gene namesi | Name:CKX9 Ordered Locus Names:Os05g0374200, LOC_Os05g31040 ORF Names:OJ1005_E12.3, OsJ_18319, OSJNBa0025P09.18 |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- extracellular space By similarity
Extracellular region or secreted
- extracellular space Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000394213 | 23 – 521 | Cytokinin dehydrogenase 9Add BLAST | 499 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 57 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 100 | Pros-8alpha-FAD histidineBy similarity | 1 | |
Glycosylationi | 278 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 412 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 418 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 472 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q75K78 |
Expressioni
Tissue specificityi
Expressed in inflorescence meristems.1 Publication
Gene expression databases
Genevisiblei | Q75K78, OS |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 4530.OS05T0374200-00 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 59 – 237 | FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST | 179 |
Sequence similaritiesi
Belongs to the oxygen-dependent FAD-linked oxidoreductase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1231, Eukaryota |
HOGENOMi | CLU_024955_1_0_1 |
InParanoidi | Q75K78 |
OMAi | PQDPVWA |
OrthoDBi | 733611at2759 |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.40.462.10, 1 hit |
InterProi | View protein in InterPro IPR016170, Cytok_DH_C_sf IPR015345, Cytokinin_DH_FAD/cytokin-bd IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR006094, Oxid_FAD_bind_N IPR006093, Oxy_OxRdtase_FAD_BS |
Pfami | View protein in Pfam PF09265, Cytokin-bind, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit PS00862, OX2_COVAL_FAD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q75K78-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRPSLLQYLK LLLLLALGGV TTMHVPKQDV PSSLEELTLD GHFSFHDVSA
60 70 80 90 100
AAQDFGNLSS FPPVAVLHPG SVADIATTIR HVFLMGEHST LTVAARGHGH
110 120 130 140 150
SLYGQSQAAE GIIISMESLQ SNTMRVNPGV SPYVDASGGE LWINVLHETL
160 170 180 190 200
KYGLAPKSWT DYLHLTVGGT LSNAGVSGQT FRHGPQISNV NELEIVTGRG
210 220 230 240 250
DVITCSPEQN SDLFHAALGG LGQFGVITRA RIPLEPAPKM VRWLRVLYLD
260 270 280 290 300
FTSFTEDQEM LISAEKTFDY IEGFVIINRT GILNNWRSSF NPQDPVRSSQ
310 320 330 340 350
FESDGKVLFC LEMTKNFNPD EADVMEQEVN TLLSQLRYMP SSLFHTDVTY
360 370 380 390 400
IEFLDRVHSS EMKLRAKGMW EVPHPWLNII IPRSMIHKFA KEVFGKILKD
410 420 430 440 450
SNNGPILLYP VNKSRWDNRT SVVIPDEEVF YLVAFLSSAL GPHNIKHTLD
460 470 480 490 500
LNYRIIEFSD KAGIGVKQYL PNYTTEQEWQ SHFGARWDTF QQRKKAYDPL
510 520
AILAPGQRIF QKASASLPLP S
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC108874 Genomic DNA Translation: AAT01339.1 AC119289 Genomic DNA Translation: AAT58842.1 AP008211 Genomic DNA Translation: BAF17285.2 AP014961 Genomic DNA Translation: BAS93715.1 CM000142 Genomic DNA Translation: EEE63503.1 |
RefSeqi | XP_015639136.1, XM_015783650.1 |
Genome annotation databases
EnsemblPlantsi | Os05t0374200-00; Os05t0374200-00; Os05g0374200 |
GeneIDi | 4338605 |
Gramenei | Os05t0374200-00; Os05t0374200-00; Os05g0374200 |
KEGGi | osa:4338605 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC108874 Genomic DNA Translation: AAT01339.1 AC119289 Genomic DNA Translation: AAT58842.1 AP008211 Genomic DNA Translation: BAF17285.2 AP014961 Genomic DNA Translation: BAS93715.1 CM000142 Genomic DNA Translation: EEE63503.1 |
RefSeqi | XP_015639136.1, XM_015783650.1 |
3D structure databases
SMRi | Q75K78 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4530.OS05T0374200-00 |
Proteomic databases
PaxDbi | Q75K78 |
Genome annotation databases
EnsemblPlantsi | Os05t0374200-00; Os05t0374200-00; Os05g0374200 |
GeneIDi | 4338605 |
Gramenei | Os05t0374200-00; Os05t0374200-00; Os05g0374200 |
KEGGi | osa:4338605 |
Phylogenomic databases
eggNOGi | KOG1231, Eukaryota |
HOGENOMi | CLU_024955_1_0_1 |
InParanoidi | Q75K78 |
OMAi | PQDPVWA |
OrthoDBi | 733611at2759 |
Gene expression databases
Genevisiblei | Q75K78, OS |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.40.462.10, 1 hit |
InterProi | View protein in InterPro IPR016170, Cytok_DH_C_sf IPR015345, Cytokinin_DH_FAD/cytokin-bd IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR006094, Oxid_FAD_bind_N IPR006093, Oxy_OxRdtase_FAD_BS |
Pfami | View protein in Pfam PF09265, Cytokin-bind, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit PS00862, OX2_COVAL_FAD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CKX9_ORYSJ | |
Accessioni | Q75K78Primary (citable) accession number: Q75K78 Secondary accession number(s): A0A0P0WLN0, Q0DIN8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 18, 2010 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 23, 2022 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - SIMILARITY comments
Index of protein domains and families